FastQCFastQC Report
Thu 26 May 2016
SRR937946_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937946_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1548816
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59760.3858431214553569No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52400.3383229512091817No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34390.22204057809320152No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA16430.10608103222074151No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA15580.10059296908089793No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA28400.056.2071151
GTATCAA51400.042.1475261
ATCAACG64950.032.909373
TATCAAC65550.032.6869352
TCAACGC65900.032.578064
CAACGCA66950.032.0671275
AACGCAG69500.031.0272466
TGGTATC12550.028.391472
ACGCAGA79700.026.9967867
CGCAGAG80350.026.7192788
GGACCGA5050.026.3353376
GTGGTAT15300.024.840991
AGGACCG6200.024.514925
GCAGAGT88150.024.139489
TATACCG1252.2571477E-422.7988765
ACCGAGT5650.022.6979968
GAGTACT57300.022.6712212-13
GTACTTT58950.021.79493914-15
CAGAGTA84000.021.54358110-11
AGAGTAC79050.021.15012710-11
Produced by FastQC (version 0.11.5)