##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937946_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1548816 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.409500547515 33.0 31.0 34.0 31.0 34.0 2 32.655376106651794 34.0 31.0 34.0 31.0 34.0 3 32.624143862150184 34.0 31.0 34.0 31.0 34.0 4 36.16667764279295 37.0 35.0 37.0 35.0 37.0 5 36.11818705385275 37.0 35.0 37.0 35.0 37.0 6 36.1820397000031 37.0 36.0 37.0 35.0 37.0 7 36.14213502443157 37.0 36.0 37.0 35.0 37.0 8 36.13231784795612 37.0 36.0 37.0 35.0 37.0 9 37.82275234759972 39.0 38.0 39.0 35.0 39.0 10-11 37.779959336680406 39.0 38.0 39.0 35.0 39.0 12-13 37.719247153955024 39.0 38.0 39.0 35.0 39.0 14-15 38.97278501771676 40.0 38.0 41.0 35.5 41.0 16-17 38.895111814444064 40.0 38.0 41.0 35.0 41.0 18-19 38.882187425749734 40.0 38.0 41.0 35.0 41.0 20-21 38.877687859629546 40.0 38.0 41.0 35.0 41.0 22-23 38.91119409923451 40.0 38.0 41.0 35.0 41.0 24-25 38.85882926054483 40.0 38.0 41.0 35.0 41.0 26-27 38.680011053604815 40.0 38.0 41.0 34.0 41.0 28-29 38.5939243267115 40.0 38.0 41.0 34.0 41.0 30-31 38.37117933957294 40.0 38.0 41.0 34.0 41.0 32-33 38.16950399530997 40.0 38.0 41.0 34.0 41.0 34-35 37.953752091920535 40.0 37.0 41.0 33.0 41.0 36-37 37.87761231805456 40.0 37.0 41.0 33.0 41.0 38-39 37.82757861489034 40.0 37.0 41.0 33.0 41.0 40-41 37.700527047757774 40.0 37.0 41.0 33.0 41.0 42-43 37.503748992779 40.0 37.0 41.0 33.0 41.0 44-45 37.244784403053686 40.0 37.0 41.0 32.0 41.0 46-47 36.87617735095712 40.0 36.0 41.0 31.0 41.0 48-49 36.48466667441452 39.0 36.0 41.0 30.0 41.0 50-51 36.16023756211196 39.0 35.0 41.0 29.5 41.0 52-53 35.66400043646243 39.0 35.0 40.0 28.0 41.0 54-55 35.31704605324325 38.0 34.5 40.0 27.5 41.0 56-57 35.014345797047554 38.0 34.0 40.0 27.0 41.0 58-59 34.59536316773587 38.0 34.0 40.0 26.0 41.0 60-61 34.14243589942252 37.0 33.5 40.0 25.5 41.0 62-63 33.66716478910342 36.5 33.0 39.0 23.5 41.0 64-65 33.19138619435749 36.0 32.5 39.0 22.5 40.0 66-67 32.640664869164574 35.5 31.5 39.0 21.5 40.0 68-69 32.07506314500883 35.0 31.0 38.0 19.5 40.0 70-71 31.44307942324976 35.0 30.5 37.5 18.0 39.5 72-73 30.940905504591896 34.0 30.0 37.0 15.5 39.0 74-75 30.35536435574013 34.0 29.0 36.0 10.0 39.0 76-77 29.20759664156362 32.5 28.5 35.0 10.5 37.0 78-79 29.902366388260454 34.0 29.5 35.0 7.5 37.0 80-81 29.691104043346662 34.0 29.0 35.0 6.5 37.0 82-83 29.196847785663373 34.0 29.0 35.0 2.0 36.5 84-85 28.639547241247506 34.0 28.5 35.0 2.0 36.0 86-87 28.04801119048357 33.0 27.0 35.0 2.0 35.5 88-89 27.36376884019793 33.0 26.0 35.0 2.0 35.0 90-91 26.769687296618837 32.5 25.0 35.0 2.0 35.0 92-93 26.05611641408663 32.0 24.0 34.0 2.0 35.0 94-95 25.22843707709631 31.5 20.5 34.0 2.0 35.0 96-97 24.31258361225607 31.0 12.5 34.0 2.0 35.0 98-99 23.1804552638919 31.0 2.0 34.0 2.0 35.0 100-101 19.714975503868764 26.5 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.06882988715076976 1101 2 0.1470833226928292 1101 3 0.28407056911340334 1101 4 0.1395888505833014 1101 5 0.12106295198334038 1101 6 0.12559423763088517 1101 7 0.10066835160489518 1101 8 0.1587788324331143 1101 9 0.14506681808654776 1101 10-11 0.18267924188721452 1101 12-13 0.217145926903612 1101 14-15 0.43680683815711774 1101 16-17 0.31256088257173076 1101 18-19 0.323366873801227 1101 20-21 0.32738755142519693 1101 22-23 0.2558725708986742 1101 24-25 0.35998391644067596 1101 26-27 0.2902516056463327 1101 28-29 0.32296748322927016 1101 30-31 0.3848564428030059 1101 32-33 0.36335799728733775 1101 34-35 0.27418731744285907 1101 36-37 0.28533779058474096 1101 38-39 0.448532556703924 1101 40-41 0.271807964517528 1101 42-43 0.3870168780543324 1101 44-45 0.3147741855839499 1101 46-47 0.4517159138370843 1101 48-49 0.4881409639952139 1101 50-51 0.5747515252209823 1101 52-53 0.4215534882762384 1101 54-55 0.4304383281129134 1101 56-57 0.5546934364306395 1101 58-59 0.6440568960371778 1101 60-61 0.6224224465034354 1101 62-63 0.6348919662851031 1101 64-65 0.6462341902867834 1101 66-67 0.7150022915977914 1101 68-69 0.9230111532047403 1101 70-71 1.0683320373019747 1101 72-73 1.0908096238140317 1101 74-75 1.090830437319834 1101 76-77 1.0757613703920725 1101 78-79 1.0390996818380493 1101 80-81 1.417241192929751 1101 82-83 1.5516012070405054 1101 84-85 1.774020301822258 1101 86-87 1.8130556761732066 1101 88-89 2.2290915195494954 1101 90-91 2.288467962201679 1101 92-93 2.4240675386474315 1101 94-95 2.6103621904436274 1101 96-97 2.74818704298956 1101 98-99 2.931943231224217 1101 100-101 3.110802551142946 1102 1 0.146677859068312 1102 2 0.14001634235829385 1102 3 0.22475817157989297 1102 4 0.08884605013422942 1102 5 0.006702473414755161 1102 6 0.005672546561328318 1102 7 0.08339771223201353 1102 8 0.11698105817854554 1102 9 0.19003638311996696 1102 10-11 0.21075944867033058 1102 12-13 0.16810291101045038 1102 14-15 0.2498787035716319 1102 16-17 0.23890452715110655 1102 18-19 0.23840780637794268 1102 20-21 0.1489797219726512 1102 22-23 0.20426659860480356 1102 24-25 0.06382279162985327 1102 26-27 0.09579598235748676 1102 28-29 0.17799895355191353 1102 30-31 0.23613227156051408 1102 32-33 0.1204864237532064 1102 34-35 0.186618477518806 1102 36-37 0.45617446631752756 1102 38-39 0.3680351986624899 1102 40-41 0.40170733691169147 1102 42-43 0.36397390357540615 1102 44-45 0.5441568702892141 1102 46-47 0.4129551089963073 1102 48-49 0.4635692637875124 1102 50-51 0.5907128893340996 1102 52-53 0.7957239655033561 1102 54-55 0.6571290870649165 1102 56-57 0.7043275956179826 1102 58-59 0.7690284237455529 1102 60-61 0.8650468850478532 1102 62-63 0.7888658429012736 1102 64-65 0.7444669725406001 1102 66-67 0.8243410682030614 1102 68-69 0.8212365529197001 1102 70-71 0.9960914187532559 1102 72-73 1.1363979266313677 1102 74-75 1.2870700241137314 1102 76-77 1.2846122692161792 1102 78-79 1.3202949598941736 1102 80-81 1.5404611682792755 1102 82-83 1.5279694300724032 1102 84-85 1.6852893872607417 1102 86-87 1.856194011446707 1102 88-89 1.7656551187712672 1102 90-91 2.056615953295818 1102 92-93 2.231776484753997 1102 94-95 2.0661234517413867 1102 96-97 2.3954479860278717 1102 98-99 2.562110895884093 1102 100-101 2.7710909743316847 1103 1 0.34041498449651186 1103 2 0.28561594175283034 1103 3 0.4048417069230794 1103 4 0.2241539586778245 1103 5 0.1488502649633503 1103 6 0.18088280752391483 1103 7 0.1885395743645546 1103 8 0.2648397537070153 1103 9 0.3157400033641977 1103 10-11 0.3638004083025024 1103 12-13 0.3146095631096486 1103 14-15 0.4891186912803249 1103 16-17 0.4723431332261896 1103 18-19 0.45040263947797143 1103 20-21 0.5391409884321803 1103 22-23 0.5643363631352898 1103 24-25 0.5233696067026372 1103 26-27 0.5507255918809761 1103 28-29 0.4070627659046764 1103 30-31 0.5021758374043443 1103 32-33 0.6455419053333173 1103 34-35 0.6171555392143233 1103 36-37 0.6224661344067997 1103 38-39 0.6131283508129073 1103 40-41 0.6543186699491272 1103 42-43 0.8136114239186512 1103 44-45 0.722833165143939 1103 46-47 0.6221576537514437 1103 48-49 0.6708302377595388 1103 50-51 0.781553591759355 1103 52-53 0.8207265261916703 1103 54-55 0.9605660998228274 1103 56-57 0.8478592969392977 1103 58-59 0.9994624888667047 1103 60-61 1.1586406119305295 1103 62-63 1.0811741042508132 1103 64-65 0.9867171916217146 1103 66-67 1.1005989352065981 1103 68-69 1.0695152013314981 1103 70-71 1.0771269611070373 1103 72-73 1.1560139371927889 1103 74-75 1.1636351737148338 1103 76-77 0.9250939062357659 1103 78-79 1.361757056016721 1103 80-81 1.5811539198242208 1103 82-83 1.6490717779390671 1103 84-85 1.7272761399665342 1103 86-87 1.7597937701729656 1103 88-89 1.9689902730385107 1103 90-91 1.72641633341577 1103 92-93 1.774578105385178 1103 94-95 1.7635764204327025 1103 96-97 2.063276067104951 1103 98-99 2.499830403776702 1103 100-101 2.649143599321725 1104 1 0.06432740166735584 1104 2 0.058726476661121296 1104 3 0.14064310901661514 1104 4 0.08366610695401278 1104 5 0.0050492470050258476 1104 6 0.03565088588749887 1104 7 -0.03214610699528464 1104 8 -0.014916320431083818 1104 9 0.10880636272269584 1104 10-11 -0.005761677551717526 1104 12-13 0.038089828162945594 1104 14-15 0.17667751198521842 1104 16-17 0.09630356729898182 1104 18-19 0.031968950819567965 1104 20-21 0.08264910328860964 1104 22-23 0.04834673478610796 1104 24-25 0.08968094829487683 1104 26-27 0.09536210393321198 1104 28-29 0.04855897256941688 1104 30-31 0.05018793766364382 1104 32-33 0.07512992031459476 1104 34-35 -0.010809504481052556 1104 36-37 -0.007342758323439114 1104 38-39 -0.020733168441871896 1104 40-41 0.03340730915012813 1104 42-43 0.18265012554162752 1104 44-45 0.24297625551174917 1104 46-47 0.2697856187951402 1104 48-49 0.36516904501969094 1104 50-51 0.4385304476126137 1104 52-53 0.42633009864917426 1104 54-55 0.39445642921877777 1104 56-57 0.5972302755237635 1104 58-59 0.27796532186391687 1104 60-61 0.322685555750752 1104 62-63 0.33078996903521585 1104 64-65 0.541965439004791 1104 66-67 0.6277717000198137 1104 68-69 0.6848998167161184 1104 70-71 0.8447347590519136 1104 72-73 0.7649738910965631 1104 74-75 0.9458209030915761 1104 76-77 0.8618268362271237 1104 78-79 1.0138649980123056 1104 80-81 0.9471195397224257 1104 82-83 0.9510644793699718 1104 84-85 1.1349636459686465 1104 86-87 1.1921235502555554 1104 88-89 1.4929459054211094 1104 90-91 1.5797823182424295 1104 92-93 1.690270028942269 1104 94-95 1.8069933031754317 1104 96-97 2.171703033397087 1104 98-99 2.497295111353772 1104 100-101 2.5906686161276404 1105 1 0.18161747838585285 1105 2 0.14998246535345316 1105 3 0.14672838383137332 1105 4 0.0926947377806755 1105 5 0.10863656335371985 1105 6 0.09881621582174915 1105 7 0.1777779456288684 1105 8 0.09808017329703489 1105 9 0.013697484768250945 1105 10-11 0.15001565143559503 1105 12-13 0.10481772156980895 1105 14-15 0.11750753529609881 1105 16-17 0.2290294912714117 1105 18-19 0.23251120074033338 1105 20-21 0.2605293626000531 1105 22-23 0.19847794045166722 1105 24-25 0.269515798031037 1105 26-27 0.335713687307603 1105 28-29 0.25174846933034445 1105 30-31 0.36919409812242776 1105 32-33 0.25444657900019507 1105 34-35 0.41523048259501394 1105 36-37 0.5140225979830291 1105 38-39 0.47262191811993404 1105 40-41 0.5662258829063731 1105 42-43 0.5911558615001553 1105 44-45 0.479397837860553 1105 46-47 0.42019828239738644 1105 48-49 0.5323459987811674 1105 50-51 0.6061411550436446 1105 52-53 0.5350848670478499 1105 54-55 0.57800748761629 1105 56-57 0.4983938149228564 1105 58-59 0.7309869077598634 1105 60-61 0.876257796749691 1105 62-63 0.8118430304107846 1105 64-65 0.819848859898535 1105 66-67 0.755546855997629 1105 68-69 0.835164040173666 1105 70-71 1.0633435240376237 1105 72-73 1.1064072368448876 1105 74-75 1.0118434166420407 1105 76-77 1.0866421342756567 1105 78-79 1.1726427457164945 1105 80-81 1.3957490323501567 1105 82-83 1.3971905385964334 1105 84-85 1.6047844150967983 1105 86-87 1.8050026413237745 1105 88-89 2.036248208980819 1105 90-91 1.9449832445089328 1105 92-93 2.3246781460349375 1105 94-95 2.3883915168043153 1105 96-97 2.3979716954077475 1105 98-99 2.7687898226434733 1105 100-101 2.70400150992392 1106 1 0.022927217008742673 1106 2 0.02755613369302523 1106 3 0.07116607324744706 1106 4 0.05252183704570257 1106 5 0.03360844972153387 1106 6 -0.06183858519747787 1106 7 -0.045206409381428614 1106 8 -0.1059166670494065 1106 9 -0.10276731514311876 1106 10-11 0.025051694553788195 1106 12-13 0.024986198486281808 1106 14-15 -0.058750331588690585 1106 16-17 0.04213985302290979 1106 18-19 -0.03234216826683678 1106 20-21 -0.09563924634805687 1106 22-23 0.09156551536444368 1106 24-25 0.1044218127548433 1106 26-27 0.05792387407925048 1106 28-29 -0.08343052458861422 1106 30-31 -0.05301536778685545 1106 32-33 -0.05257439278297227 1106 34-35 -0.14492472286593738 1106 36-37 -0.01643535449469624 1106 38-39 0.06738610507066056 1106 40-41 -0.08371709308664066 1106 42-43 -0.029604519297286913 1106 44-45 0.11591418322495173 1106 46-47 -0.2265660699722858 1106 48-49 0.08832798025728295 1106 50-51 0.017602752808521416 1106 52-53 -0.013747108281968679 1106 54-55 -0.004412690155959353 1106 56-57 0.2630265621065675 1106 58-59 0.3083523227565408 1106 60-61 0.19821200150192197 1106 62-63 0.35642400450070966 1106 64-65 0.28841451831903697 1106 66-67 0.4388378734455429 1106 68-69 0.2888787956950978 1106 70-71 0.2717877057677818 1106 72-73 0.3462463024251541 1106 74-75 0.5128880979677604 1106 76-77 0.5168844730263338 1106 78-79 0.8677488870085526 1106 80-81 0.8752019888722913 1106 82-83 0.8990602279275173 1106 84-85 0.8162086038989997 1106 86-87 1.0543699897491834 1106 88-89 0.9866323906806294 1106 90-91 0.9031588101582457 1106 92-93 0.9118799176869921 1106 94-95 1.1281877100439957 1106 96-97 1.3449236237525106 1106 98-99 1.7509733299196313 1106 100-101 2.0481817983599235 1107 1 0.06927956957763826 1107 2 0.07302931494847797 1107 3 0.1405840662228428 1107 4 0.1340132711546218 1107 5 0.13315651327911127 1107 6 -0.014532518111515458 1107 7 -0.03030909629850953 1107 8 0.043045136136072415 1107 9 0.059867684791107934 1107 10-11 0.049390233243897796 1107 12-13 0.06113508465004003 1107 14-15 0.10202309799794307 1107 16-17 0.14416647810129035 1107 18-19 0.12297060624582201 1107 20-21 0.13218415057112054 1107 22-23 0.08499968421502757 1107 24-25 0.09089021155221388 1107 26-27 0.2285359103218667 1107 28-29 0.19026873304877512 1107 30-31 0.1120631145135178 1107 32-33 0.09300373361014636 1107 34-35 0.15160923740984344 1107 36-37 0.20307647521939742 1107 38-39 0.2785322872162439 1107 40-41 0.3857021057347012 1107 42-43 0.37577019184779914 1107 44-45 0.4407246827082716 1107 46-47 0.5658254440878849 1107 48-49 0.40950888345781067 1107 50-51 0.34427902482982375 1107 52-53 0.3903691381900174 1107 54-55 0.3898150714870354 1107 56-57 0.5651841493623024 1107 58-59 0.4326496434399374 1107 60-61 0.7248776401544674 1107 62-63 0.6116860604022705 1107 64-65 0.6097320840595657 1107 66-67 0.72799461441668 1107 68-69 0.9008757569633303 1107 70-71 1.0285112104091958 1107 72-73 0.9700397256683075 1107 74-75 1.082201401591 1107 76-77 0.9776752960271509 1107 78-79 1.2116230800052925 1107 80-81 1.1785875292447408 1107 82-83 1.3349735356433214 1107 84-85 1.5596599469190515 1107 86-87 1.5177822487444281 1107 88-89 1.4541527475525626 1107 90-91 1.5985233921974284 1107 92-93 1.7599215873378213 1107 94-95 1.9489750689782888 1107 96-97 1.9542900138297874 1107 98-99 2.092054710635157 1107 100-101 2.329038299769664 1108 1 -0.03052061585085397 1108 2 -0.04846239820786025 1108 3 0.05469773324969651 1108 4 -0.02856325874527954 1108 5 -0.16127359810169395 1108 6 -0.08306435642324317 1108 7 -0.049474046002004 1108 8 -0.08848078196646014 1108 9 -0.07904037965148092 1108 10-11 -0.07667904688283755 1108 12-13 -0.09874113847635613 1108 14-15 -0.013056910895272722 1108 16-17 -0.027615731438558555 1108 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-0.7113532955919766 2316 52-53 -0.6222979390550236 2316 54-55 -0.5775065271386808 2316 56-57 -0.5348929302573779 2316 58-59 -0.6704126976751539 2316 60-61 -0.7820413398625377 2316 62-63 -0.9134896681907421 2316 64-65 -1.0964329915284665 2316 66-67 -0.8131305451895443 2316 68-69 -0.798345333195698 2316 70-71 -1.3240126400325636 2316 72-73 -1.341822177309993 2316 74-75 -1.3438649393408326 2316 76-77 -1.339503841695315 2316 78-79 -1.3637483028219712 2316 80-81 -1.5969969416599739 2316 82-83 -1.7375605198043402 2316 84-85 -1.830823168132774 2316 86-87 -1.878807957317651 2316 88-89 -1.7505774020847191 2316 90-91 -1.8707391859619733 2316 92-93 -2.4605731630994256 2316 94-95 -2.4141538739642563 2316 96-97 -2.727523462583463 2316 98-99 -3.305867601921303 2316 100-101 -3.968825468647344 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 6.0 4 16.0 5 30.0 6 60.0 7 147.0 8 370.0 9 714.0 10 1164.0 11 1934.0 12 2938.0 13 4393.0 14 6086.0 15 8444.0 16 10754.0 17 14712.0 18 14931.0 19 12194.0 20 11243.0 21 11250.0 22 12137.0 23 13504.0 24 15089.0 25 17396.0 26 20201.0 27 24345.0 28 29530.0 29 35981.0 30 43270.0 31 52820.0 32 66167.0 33 85228.0 34 116853.0 35 167975.0 36 247021.0 37 316791.0 38 174009.0 39 9107.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.02454261836138 18.672263199760334 15.128846809433789 23.174347372444497 2 15.959629224257336 19.14893342244349 40.23239670808997 24.6590406452092 3 20.24796056599217 22.354030505309566 29.224827824892134 28.173181103806126 4 13.34574281257425 16.11566512742637 35.6536218634105 34.884970196588874 5 14.221185439908988 36.615425142803275 33.935753214243974 15.227636203043756 6 34.04041427961744 35.20808569681607 16.168100779373056 14.583399244193442 7 29.935770291629222 32.01748948874495 19.113309779857644 18.933430439768184 8 26.70280181945552 35.48700135264703 18.41272198422665 19.397474843670807 9 25.90055887852398 16.9291252156486 19.73513961632628 37.43517628950114 10-11 25.7012453383746 26.1899412196155 26.194073408332557 21.914740033677337 12-13 26.349417877914483 23.22177069451762 26.97144141072923 23.45737001683867 14-15 22.8972001838824 24.87603433848824 25.309720457433293 26.917045020196074 16-17 22.61127209429655 28.13439427278644 26.28624058635758 22.96809304655944 18-19 22.542090216010166 26.43067349510852 28.639715757068622 22.387520531812687 20-21 22.873536946932365 25.615599270668692 29.263288860652263 22.247574921746676 22-23 22.781886292496978 25.488986425760064 29.076759279346287 22.65236800239667 24-25 22.63008236117532 25.689539175148568 29.07279756816298 22.607580895513138 26-27 22.200491697518846 26.072561224865403 28.89972509110353 22.82722198651222 28-29 22.324748799483476 26.35025220935394 28.865630926919817 22.459368064242767 30-31 22.45130065608244 25.928669960153393 28.71144349436295 22.908585889401216 32-33 22.119656331627777 25.973286379656507 28.843291135072207 23.06376615364351 34-35 22.79445832621666 26.10878839411376 28.61900169411792 22.477751585551665 36-37 22.88138520759098 25.686242034885243 28.611005377713596 22.821367379810184 38-39 22.371974530519463 26.04696742696252 28.571733009583344 23.009325032934676 40-41 22.983737642752526 25.93749757827656 28.489897215599257 22.588867563371657 42-43 22.599812985858993 25.916117441780873 28.42427670860278 23.059792863757355 44-45 22.586705486910923 26.210057426454135 28.59480336919944 22.6084337174355 46-47 22.95800138043336 26.4619325163079 27.84598160240033 22.734084500858412 48-49 22.77491482985502 26.44334058728257 27.81794298402356 22.963801598838852 50-51 22.892067230710428 26.756147922025598 27.732635768225535 22.61914907903844 52-53 23.117562060309293 26.44675029183583 27.672719031828187 22.762968616026694 54-55 22.833732347806325 26.621141568785443 27.776701686966042 22.768424396442185 56-57 22.86859769010651 26.75004648712307 27.659224853049043 22.722130969721388 58-59 22.846387175752316 26.444490501131185 27.575870858772124 23.133251464344376 60-61 22.578989369944523 26.684383425791058 27.741900910114563 22.994726294149853 62-63 22.613176775033317 26.737391659177074 27.838329407754053 22.811102158035556 64-65 22.397108500945237 26.710274170721377 27.76081858658485 23.131798741748536 66-67 22.280424518784553 26.718954037367336 27.742060449537952 23.258560994310155 68-69 22.792949058780117 26.370911429354514 27.789286580155338 23.046852931710028 70-71 22.600651077984732 26.521194254191588 27.4613640354955 23.416790632328176 72-73 22.658695416369664 26.617073945517088 27.451711500914243 23.272519137198998 74-75 22.650882997076476 26.919627638144235 27.243843038811583 23.185646325967706 76-77 22.678775270916624 26.573911943058437 27.47795735581244 23.2693554302125 78-79 22.757383704713792 26.716924411937892 27.407581016725036 23.11811086662328 80-81 22.80643588808218 26.750096041166955 27.25448165210177 23.188986418649097 82-83 22.792273238812783 26.85219321551731 27.260025348673388 23.095508196996512 84-85 22.770876863117824 26.951350381082012 27.245458912618453 23.03231384318172 86-87 22.90658833533326 26.847690042423018 27.097108061664294 23.148613560579427 88-89 22.829388919916195 26.915415979313217 26.929555821708856 23.325639279061736 90-91 22.833196449279292 26.89012988488826 26.951921413056674 23.324752252775777 92-93 22.825364450216632 26.625066866046126 27.130391776113918 23.419176907623328 94-95 22.638699003268634 26.561930511278803 27.192930067390343 23.606440418062224 96-97 22.887226754191843 26.231016492323306 27.224746200554424 23.657010552930423 98-99 22.63019826176338 26.41361663882811 26.945476239515344 24.01070885989317 100-101 23.000378686503424 26.022865175014182 27.044899047601827 23.931857090880566 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 305.0 1 229.0 2 225.0 3 317.0 4 286.0 5 249.5 6 501.5 7 1239.5 8 1964.0 9 2207.0 10 1824.5 11 1392.5 12 1356.5 13 1433.5 14 1621.5 15 1703.5 16 1745.5 17 1891.0 18 2069.0 19 2295.5 20 2508.5 21 2848.0 22 3410.5 23 4117.0 24 5091.5 25 6474.0 26 8286.5 27 10320.0 28 12106.0 29 13762.0 30 15668.5 31 18651.5 32 22633.0 33 24727.5 34 25613.5 35 27683.0 36 30679.0 37 33676.0 38 35834.5 39 37652.5 40 40393.5 41 43676.5 42 45567.5 43 47588.5 44 50216.0 45 53092.0 46 54981.5 47 56787.0 48 58234.5 49 58206.0 50 58234.0 51 57435.0 52 57277.0 53 58350.0 54 59237.5 55 56412.5 56 51650.5 57 47532.0 58 44015.0 59 39511.5 60 32267.5 61 26285.0 62 21892.5 63 16601.5 64 11933.5 65 9016.0 66 6725.0 67 5036.5 68 3819.0 69 2939.5 70 2237.0 71 1488.5 72 1034.0 73 847.5 74 642.5 75 444.5 76 294.5 77 180.5 78 116.0 79 70.0 80 37.0 81 22.0 82 16.5 83 8.5 84 5.5 85 6.0 86 1.5 87 0.5 88 0.5 89 0.5 90 1.0 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.011040691728391236 3 0.006585675767812316 4 0.0 5 4.519581409282962E-4 6 4.519581409282962E-4 7 0.0 8 6.456544870404231E-5 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0018078325637131847 26-27 0.01956333095732482 28-29 2.259790704641481E-4 30-31 9.684817305606348E-5 32-33 0.003583382403074348 34-35 0.014365812336649414 36-37 0.007069916633092634 38-39 0.019111372816396525 40-41 0.021435728969742048 42-43 0.017432671150091426 44-45 0.009006880094213904 46-47 0.0017432671150091424 48-49 2.9054451916819043E-4 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 2.259790704641481E-4 68-69 9.684817305606348E-5 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 6.456544870404231E-5 82-83 0.0010976126279687193 84-85 0.004390450511874877 86-87 6.779372113924443E-4 88-89 6.456544870404231E-5 90-91 0.003873926922242539 92-93 6.779372113924443E-4 94-95 2.259790704641481E-4 96-97 2.9054451916819043E-4 98-99 3.2282724352021157E-4 100-101 0.002679466121217756 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1548816.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.625129204380876 #Duplication Level Percentage of deduplicated Percentage of total 1 78.88699375296781 47.03647195065892 2 12.380792988825261 14.76412763222798 3 3.7994418680782767 6.796266368661044 4 1.6221144820737912 3.8687514231178866 5 0.8707599879601235 2.595958839406375 6 0.5291648166969113 1.8930912339579518 7 0.34187875159960307 1.4269195315451138 8 0.26934001572986455 1.284754659024252 9 0.1864334196314279 1.00045050601846 >10 1.0249913428378876 11.026570019707224 >50 0.0573663683741299 2.332214133637835 >100 0.026488556329945935 2.985500999377768 >500 0.0028224325966260807 1.1703129496032911 >1k 0.0011941060985725727 1.092641607336605 >5k 2.1711019974046778E-4 0.7259681457192916 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5976 0.3858431214553569 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5240 0.3383229512091817 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3439 0.22204057809320152 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 1643 0.10608103222074151 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 1558 0.10059296908089793 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 2.5826179481616923E-4 14-15 0.0 0.0 0.0 6.456544870404231E-5 2.5826179481616923E-4 16-17 0.0 0.0 0.0 1.2913089740808462E-4 2.5826179481616923E-4 18-19 3.2282724352021154E-5 0.0 0.0 1.2913089740808462E-4 2.5826179481616923E-4 20-21 6.456544870404231E-5 0.0 0.0 1.2913089740808462E-4 2.5826179481616923E-4 22-23 6.456544870404231E-5 0.0 0.0 1.9369634611212695E-4 2.5826179481616923E-4 24-25 1.2913089740808462E-4 0.0 0.0 5.165235896323385E-4 2.5826179481616923E-4 26-27 1.2913089740808462E-4 0.0 0.0 0.0010007644549126558 2.5826179481616923E-4 28-29 3.2282724352021157E-4 0.0 0.0 0.002485769775105629 2.5826179481616923E-4 30-31 3.2282724352021157E-4 0.0 0.0 0.009103728267269967 2.5826179481616923E-4 32-33 3.2282724352021157E-4 0.0 0.0 0.021887687110670344 2.5826179481616923E-4 34-35 3.2282724352021157E-4 0.0 0.0 0.04274232704207601 2.5826179481616923E-4 36-37 3.2282724352021157E-4 0.0 0.0 0.08112648629662916 2.5826179481616923E-4 38-39 4.1967541657627504E-4 0.0 0.0 0.13894484561109904 2.5826179481616923E-4 40-41 4.519581409282962E-4 0.0 0.0 0.20176702720013223 2.5826179481616923E-4 42-43 4.842408652803173E-4 0.0 0.0 0.2658159523145422 2.5826179481616923E-4 44-45 5.810890383363809E-4 0.0 0.0 0.3399048047024308 2.5826179481616923E-4 46-47 5.810890383363809E-4 0.0 0.0 0.4294247993305854 2.5826179481616923E-4 48-49 5.810890383363809E-4 0.0 0.0 0.5237872026115432 2.5826179481616923E-4 50-51 5.810890383363809E-4 0.0 0.0 0.625736046115226 3.2282724352021157E-4 52-53 5.810890383363809E-4 0.0 0.0 0.7199047530500717 3.2282724352021157E-4 54-55 5.810890383363809E-4 0.0 0.0 0.8216921829319945 3.2282724352021157E-4 56-57 5.810890383363809E-4 0.0 0.0 0.941461090277993 3.2282724352021157E-4 58-59 5.810890383363809E-4 0.0 0.0 1.069978615923389 3.2282724352021157E-4 60-61 6.456544870404231E-4 0.0 0.0 1.2055014927531742 3.2282724352021157E-4 62-63 6.456544870404231E-4 0.0 0.0 1.3355040237187632 3.551099678722327E-4 64-65 6.779372113924443E-4 0.0 0.0 1.469186785260483 3.873926922242539E-4 66-67 7.102199357444654E-4 0.0 0.0 1.6285665953864115 3.873926922242539E-4 68-69 7.102199357444654E-4 0.0 0.0 1.8056373384572475 3.873926922242539E-4 70-71 7.425026600964866E-4 0.0 0.0 1.9859686366876375 3.873926922242539E-4 72-73 8.070681088005289E-4 0.0 0.0 2.1721108253013917 4.1967541657627504E-4 74-75 8.393508331525501E-4 0.0 0.0 2.351376793628165 4.519581409282962E-4 76-77 8.393508331525501E-4 0.0 0.0 2.543200741727875 4.519581409282962E-4 78-79 8.393508331525501E-4 0.0 0.0 2.7623035919050425 4.519581409282962E-4 80-81 0.0010976126279687193 0.0 0.0 3.000324118552494 4.519581409282962E-4 82-83 0.0011621780766727617 0.0 0.0 3.2432193365771016 4.519581409282962E-4 84-85 0.0011944608010247828 0.0 0.0 3.477236805404903 4.519581409282962E-4 86-87 0.0012590262497288252 0.0 0.0 3.71693603371866 4.842408652803173E-4 88-89 0.0012913089740808463 0.0 0.0 3.978910341835311 5.165235896323385E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2840 0.0 56.207115 1 GTATCAA 5140 0.0 42.147526 1 ATCAACG 6495 0.0 32.90937 3 TATCAAC 6555 0.0 32.686935 2 TCAACGC 6590 0.0 32.57806 4 CAACGCA 6695 0.0 32.067127 5 AACGCAG 6950 0.0 31.027246 6 TGGTATC 1255 0.0 28.39147 2 ACGCAGA 7970 0.0 26.996786 7 CGCAGAG 8035 0.0 26.719278 8 GGACCGA 505 0.0 26.335337 6 GTGGTAT 1530 0.0 24.84099 1 AGGACCG 620 0.0 24.51492 5 GCAGAGT 8815 0.0 24.13948 9 TATACCG 125 2.2571477E-4 22.798876 5 ACCGAGT 565 0.0 22.697996 8 GAGTACT 5730 0.0 22.67122 12-13 GTACTTT 5895 0.0 21.794939 14-15 CAGAGTA 8400 0.0 21.543581 10-11 AGAGTAC 7905 0.0 21.150127 10-11 >>END_MODULE