FastQCFastQC Report
Thu 26 May 2016
SRR937945_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937945_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1573566
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63630.4043681675887761No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51480.3271550097040734No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32210.20469430579969317No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA16780.10663677278233007No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA16730.10631902316140537No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA30100.054.252571
GTATCAA54050.042.31561
ATCAACG66300.034.3281253
TATCAAC67700.033.828792
TCAACGC67750.033.7336924
CAACGCA68200.033.30215
AACGCAG70900.032.0338946
TGGTATC14950.029.2398242
GTGGTAT16800.028.706011
ACGCAGA79750.028.1340877
CGCAGAG81450.027.430168
GCAGAGT89200.024.567319
GAGTACT58050.023.13110212-13
GTACTTT60400.022.17978714-15
GTACATG49350.022.060151
TACATGG47800.021.96812
GTAAGGT9200.021.69154
CAGAGTA85600.021.68512510-11
TAAGGTG10400.021.4729945
TAGACCG1353.7888702E-421.117655
Produced by FastQC (version 0.11.5)