FastQCFastQC Report
Thu 26 May 2016
SRR937945_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937945_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1573566
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62480.3970599263075079No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52730.335098750227191No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33030.20990539958285828No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA17170.10911521982554274No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA16270.10339572664889811No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA15890.10098082952987038No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA30000.055.8998951
GTATCAA50850.039.9862171
TGGTATC12750.032.789142
GTGGTAT14250.032.6714741
ATCAACG65300.030.8419043
TCAACGC65300.030.8399454
TATCAAC65650.030.6823542
CAACGCA66950.030.0089445
AACGCAG69850.028.899046
CGCAGAG80350.024.7678938
ACGCAGA81100.024.5388457
GCAGAGT90450.022.0022139
GAGTACT59500.021.95352412-13
GTACTTT61200.021.38251114-15
TAAGGTG8750.021.1698365
GTAAGGT9050.020.9928954
CAGAGTA85250.020.7542310-11
AGAGTAC80950.020.53647810-11
TACATGG47900.019.8359052
AGTACTT60700.019.75883112-13
Produced by FastQC (version 0.11.5)