##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937945_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1573566 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.43114302164638 33.0 31.0 34.0 31.0 34.0 2 32.65852719237706 34.0 31.0 34.0 31.0 34.0 3 32.719865579200366 34.0 31.0 34.0 31.0 34.0 4 36.193389409786434 37.0 35.0 37.0 35.0 37.0 5 36.14673423294606 37.0 35.0 37.0 35.0 37.0 6 36.19097197067044 37.0 37.0 37.0 35.0 37.0 7 36.15150810325083 37.0 36.0 37.0 35.0 37.0 8 36.13937578722469 37.0 36.0 37.0 35.0 37.0 9 37.84006263480528 39.0 38.0 39.0 35.0 39.0 10-11 37.81971807982633 39.0 38.0 39.0 35.0 39.0 12-13 37.768311275154645 39.0 38.0 39.0 35.0 39.0 14-15 39.07129729544232 40.0 38.0 41.0 36.0 41.0 16-17 39.01384276223558 40.0 38.0 41.0 36.0 41.0 18-19 39.03621773729224 40.0 38.5 41.0 36.0 41.0 20-21 38.955872521394085 40.0 38.0 41.0 35.0 41.0 22-23 38.811183325008294 40.0 38.0 41.0 35.0 41.0 24-25 38.770112280006046 40.0 38.0 41.0 34.5 41.0 26-27 38.731076421325824 40.0 38.0 41.0 35.0 41.0 28-29 38.67515534778967 40.0 38.0 41.0 34.5 41.0 30-31 38.48232994358038 40.0 38.0 41.0 34.0 41.0 32-33 38.30571771377877 40.0 38.0 41.0 34.0 41.0 34-35 38.10079526375125 40.0 38.0 41.0 33.5 41.0 36-37 37.969099484864316 40.0 37.5 41.0 33.0 41.0 38-39 37.96434181979021 40.0 38.0 41.0 33.0 41.0 40-41 37.902296757809964 40.0 38.0 41.0 33.0 41.0 42-43 37.7437587619458 40.0 37.0 41.0 33.0 41.0 44-45 37.53646526424694 40.0 37.0 41.0 33.0 41.0 46-47 37.217190445141796 40.0 37.0 41.0 31.5 41.0 48-49 36.866023414334066 40.0 36.0 41.0 31.0 41.0 50-51 36.556497471348514 39.5 36.0 41.0 30.5 41.0 52-53 36.121359701467874 39.0 35.0 41.0 29.5 41.0 54-55 35.88183717746824 39.0 35.0 40.0 29.0 41.0 56-57 35.6184033589948 38.5 35.0 40.0 28.0 41.0 58-59 35.2573482777335 38.0 34.5 40.0 27.5 41.0 60-61 34.88519896845763 38.0 34.0 40.0 27.0 41.0 62-63 34.49818882716073 37.0 34.0 40.0 26.0 41.0 64-65 34.08144272308883 36.5 33.5 39.5 26.0 41.0 66-67 33.64571362116365 36.0 33.0 39.0 25.5 40.5 68-69 33.171874265203996 35.5 33.0 39.0 24.5 40.0 70-71 32.63517132424061 35.0 32.0 38.0 22.5 40.0 72-73 32.20206079694147 35.0 32.0 37.0 22.0 39.5 74-75 31.741058525667178 35.0 31.0 37.0 20.5 39.0 76-77 30.475989885394068 33.5 29.5 35.5 20.0 37.5 78-79 31.20958987420928 34.5 31.0 36.0 20.0 38.0 80-81 31.053829963280855 34.5 31.0 35.5 20.0 37.0 82-83 30.671753838097672 34.0 31.0 35.0 18.0 37.0 84-85 30.277185068818213 34.0 31.0 35.0 16.0 36.0 86-87 29.83016791160968 34.0 30.0 35.0 8.5 36.0 88-89 29.332647629651376 34.0 29.5 35.0 4.5 35.5 90-91 28.957354505626075 34.0 29.0 35.0 2.0 35.0 92-93 28.507092807038283 34.0 29.0 35.0 2.0 35.0 94-95 28.0385344497784 33.5 28.5 35.0 2.0 35.0 96-97 27.39016031103875 33.0 27.0 35.0 2.0 35.0 98-99 26.624227074047102 33.0 25.5 35.0 2.0 35.0 100-101 24.11684320835605 31.0 13.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.1901032574795849 1101 2 0.13568628749622036 1101 3 0.1245272246602056 1101 4 0.04458911776423946 1101 5 0.033061610709403055 1101 6 0.05166582652495322 1101 7 0.10003264443435711 1101 8 0.11035541015074557 1101 9 0.04828406591376222 1101 10-11 0.053084062428879975 1101 12-13 0.19187215425569093 1101 14-15 0.078238813566891 1101 16-17 0.15851925819939083 1101 18-19 0.19592804099494998 1101 20-21 0.24293704572323094 1101 22-23 0.28371390127350793 1101 24-25 0.1673543113747158 1101 26-27 0.14984618034558395 1101 28-29 0.3393229938394242 1101 30-31 0.12935462303686762 1101 32-33 0.16760572745680236 1101 34-35 0.13357547040827455 1101 36-37 0.28759696818825375 1101 38-39 0.4355109538145996 1101 40-41 0.5017320691929896 1101 42-43 0.550914286568208 1101 44-45 0.42301810984717747 1101 46-47 0.44936992918638907 1101 48-49 0.3644229588525647 1101 50-51 0.29484486965642986 1101 52-53 0.37723655867770134 1101 54-55 0.33421354176657303 1101 56-57 0.30766439644472143 1101 58-59 0.33763663645428466 1101 60-61 0.40945233864790964 1101 62-63 0.4828654668826289 1101 64-65 0.6080998342659498 1101 66-67 0.5124255981344064 1101 68-69 0.523548123879614 1101 70-71 0.542891223567814 1101 72-73 0.39189570806192364 1101 74-75 0.403518160912693 1101 76-77 0.3714044760375437 1101 78-79 0.6934525406260263 1101 80-81 0.7082372055719084 1101 82-83 0.590275355593679 1101 84-85 0.6105467425869975 1101 86-87 0.6652443966691699 1101 88-89 0.6138953635216744 1101 90-91 0.729928550845262 1101 92-93 0.9624005306762875 1101 94-95 0.7230098474723441 1101 96-97 0.8355084894430753 1101 98-99 1.0491979538368312 1101 100-101 1.10787414294332 1102 1 0.18379999180233142 1102 2 0.1368756078311293 1102 3 0.1626968778374902 1102 4 -0.04021545458021336 1102 5 0.10879370760752494 1102 6 -0.011278765061739193 1102 7 0.07151977921152053 1102 8 0.07192684532706295 1102 9 0.06381127915700802 1102 10-11 0.1382342906277927 1102 12-13 0.12237331121206552 1102 14-15 0.10896431658422756 1102 16-17 0.2140163971816733 1102 18-19 0.16975837020462592 1102 20-21 0.17244901047954642 1102 22-23 0.306953203004646 1102 24-25 0.15724024494273436 1102 26-27 0.06501540860833899 1102 28-29 -0.01062075452311717 1102 30-31 0.14528759662392332 1102 32-33 0.08216796642608415 1102 34-35 0.10671140562752157 1102 36-37 0.12618206887978545 1102 38-39 0.15439521194246453 1102 40-41 0.14506872698709827 1102 42-43 0.04475500746982419 1102 44-45 0.018776009844756913 1102 46-47 -0.0614178359173394 1102 48-49 -0.031895476052952176 1102 50-51 0.09668961513703067 1102 52-53 -0.04895534857204353 1102 54-55 0.10457498330559645 1102 56-57 0.18283882055344947 1102 58-59 0.05628157743960571 1102 60-61 0.018892045919230327 1102 62-63 0.03279556514136317 1102 64-65 0.08858965282965414 1102 66-67 0.029440892855561174 1102 68-69 0.2860021923811189 1102 70-71 0.3683317146192877 1102 72-73 0.33179121871890516 1102 74-75 0.40890290670152396 1102 76-77 0.19867562213989132 1102 78-79 0.3532754415372814 1102 80-81 0.4250856167658732 1102 82-83 0.5532974445439223 1102 84-85 0.3247789572723079 1102 86-87 0.35995828388849205 1102 88-89 0.2934915406119565 1102 90-91 0.5649252153763484 1102 92-93 0.5457727227728775 1102 94-95 0.46502816943425884 1102 96-97 0.49049396969556014 1102 98-99 0.501174466851765 1102 100-101 0.6432589547810394 1103 1 0.17501511657582114 1103 2 0.20159086978025442 1103 3 0.12948997146668972 1103 4 0.09048800723013528 1103 5 0.08648110404858755 1103 6 0.11713323514109675 1103 7 0.11039531171521588 1103 8 0.10974571367356134 1103 9 0.010732325367207807 1103 10-11 0.0857525817964131 1103 12-13 0.12140614711577058 1103 14-15 0.22551723365776155 1103 16-17 0.251044501067625 1103 18-19 0.2561371977408342 1103 20-21 0.2601386996824573 1103 22-23 0.1786835794119952 1103 24-25 0.260831906224233 1103 26-27 0.17335317360328162 1103 28-29 0.2994991235487632 1103 30-31 0.22893418017037703 1103 32-33 0.37620383839722393 1103 34-35 0.3024915969452806 1103 36-37 0.2977666880451082 1103 38-39 0.17081381391250972 1103 40-41 0.3164934162615154 1103 42-43 0.38331055016741544 1103 44-45 0.35924572696821855 1103 46-47 0.3822198799717569 1103 48-49 0.5071521219431148 1103 50-51 0.5851668615343897 1103 52-53 0.5559536755912333 1103 54-55 0.684345975837438 1103 56-57 0.7555579450911551 1103 58-59 0.7448382686852 1103 60-61 0.7098886431832554 1103 62-63 0.8609342109209308 1103 64-65 0.7934201856562382 1103 66-67 0.8793690110875261 1103 68-69 0.7264644981193911 1103 70-71 0.738468859918413 1103 72-73 0.7805902492577133 1103 74-75 0.8850443141972555 1103 76-77 0.8786725969078084 1103 78-79 0.8059682664929362 1103 80-81 1.0171616757812991 1103 82-83 0.9386743048124409 1103 84-85 1.144659542953768 1103 86-87 1.3113788210535553 1103 88-89 1.2470306070634756 1103 90-91 1.2554745290430738 1103 92-93 1.2402630730193387 1103 94-95 1.3840522850414665 1103 96-97 1.4975350023312224 1103 98-99 1.7409542316898161 1103 100-101 1.772605396297628 1104 1 0.14984444043596312 1104 2 0.11476911185171446 1104 3 0.1255572700692511 1104 4 0.03475290949195653 1104 5 0.11562772946928845 1104 6 0.05472694738845263 1104 7 0.0781144387444499 1104 8 0.05095225618072163 1104 9 0.12785289761014695 1104 10-11 0.11518419444878703 1104 12-13 0.20565445198233334 1104 14-15 0.16678354800377093 1104 16-17 0.12608443395996005 1104 18-19 0.13276159436145463 1104 20-21 0.17822843251253317 1104 22-23 0.20412475882258008 1104 24-25 0.21216273743630865 1104 26-27 0.29115245201643347 1104 28-29 0.3239796695492103 1104 30-31 0.18350309465258619 1104 32-33 0.15907375977991478 1104 34-35 0.2119111491501684 1104 36-37 0.23124042739990358 1104 38-39 0.17678385781727002 1104 40-41 0.1737394971260855 1104 42-43 0.3071765274247511 1104 44-45 0.3384687909828301 1104 46-47 0.5260517322010827 1104 48-49 0.53716737702198 1104 50-51 0.5732922575483173 1104 52-53 0.6270631918799765 1104 54-55 0.5771316168873497 1104 56-57 0.45793641547880526 1104 58-59 0.4912004484927621 1104 60-61 0.41743156364754697 1104 62-63 0.6167960331174527 1104 64-65 0.5463087158687614 1104 66-67 0.4758154630678959 1104 68-69 0.4995669258352464 1104 70-71 0.6200575831552158 1104 72-73 0.5995731732797509 1104 74-75 0.5888436407542663 1104 76-77 0.4185834573081273 1104 78-79 0.6100043548116396 1104 80-81 0.7848087502047925 1104 82-83 0.7653584120720858 1104 84-85 0.9828312672428012 1104 86-87 0.82473894903465 1104 88-89 0.8065095027083764 1104 90-91 0.8698188727707219 1104 92-93 0.9438800240680401 1104 94-95 0.7203825062951914 1104 96-97 0.9789212765138267 1104 98-99 1.1376556421828106 1104 100-101 1.2304219820241116 1105 1 0.1441584988950595 1105 2 0.09243172875337535 1105 3 0.07293199365083325 1105 4 0.014739398870688092 1105 5 0.052873288622350856 1105 6 0.08491676135319892 1105 7 0.051354360995652826 1105 8 0.012478538216647905 1105 9 -0.06710947299067982 1105 10-11 -0.0308783946257023 1105 12-13 0.06164202555611098 1105 14-15 0.10285956786750461 1105 16-17 0.054824080030343225 1105 18-19 0.12910224601133535 1105 20-21 0.10920204498427921 1105 22-23 0.2040768596025373 1105 24-25 0.12718838062590265 1105 26-27 -0.03591102547454739 1105 28-29 -0.008461255696452952 1105 30-31 0.0851481287389646 1105 32-33 0.07134139629174285 1105 34-35 0.13736114521559273 1105 36-37 0.12760134118016708 1105 38-39 0.11593493157712231 1105 40-41 0.15958128600228605 1105 42-43 0.14048895236837922 1105 44-45 0.32762189855878177 1105 46-47 0.3351243006598992 1105 48-49 0.35405512139829654 1105 50-51 0.18573244529805777 1105 52-53 0.14585204109361172 1105 54-55 0.35168101257786333 1105 56-57 0.3257625093772418 1105 58-59 0.2695667657612333 1105 60-61 0.24882687933192216 1105 62-63 0.33979028178967496 1105 64-65 0.2750776716999823 1105 66-67 0.20345954753322104 1105 68-69 0.2715483911180101 1105 70-71 0.44389660680184306 1105 72-73 0.2887750924418384 1105 74-75 0.22208596556952287 1105 76-77 0.2912542151764299 1105 78-79 0.3618704285924039 1105 80-81 0.4456683967465658 1105 82-83 0.6891853468679408 1105 84-85 0.6705089647957223 1105 86-87 0.5336116713031878 1105 88-89 0.3871198984357669 1105 90-91 0.28101866079766324 1105 92-93 0.32315232086985546 1105 94-95 0.47346202698366113 1105 96-97 0.5702342967763379 1105 98-99 0.6233033631873433 1105 100-101 0.5974860601561041 1106 1 -0.09179144932595307 1106 2 0.008590512676093454 1106 3 0.04519452996155593 1106 4 -0.031064633535109465 1106 5 -0.01632998784755557 1106 6 -0.025469693113066683 1106 7 -0.044789319163463404 1106 8 -0.04847711130125987 1106 9 -0.05389068899319227 1106 10-11 0.11404446028470261 1106 12-13 -0.035155341005499 1106 14-15 -0.25725228970849656 1106 16-17 -0.1261973306761348 1106 18-19 -0.0635434548369389 1106 20-21 0.06190857212805412 1106 22-23 0.1143907860950577 1106 24-25 0.08909268856322683 1106 26-27 0.10188539523256424 1106 28-29 0.055866009961548 1106 30-31 0.009854184824767742 1106 32-33 -0.030047290092070966 1106 34-35 0.03085549812773536 1106 36-37 -0.03638851985773073 1106 38-39 0.04241941844171038 1106 40-41 -0.03524997430210419 1106 42-43 -0.06623203550748968 1106 44-45 0.03342208025257065 1106 46-47 -0.01678684883371062 1106 48-49 0.05881486313889184 1106 50-51 -0.04833532723328915 1106 52-53 -0.04108376417251236 1106 54-55 -0.3513804903961528 1106 56-57 0.00479257857789861 1106 58-59 -0.030553241865426628 1106 60-61 -0.11776165152798512 1106 62-63 -0.2943485232932659 1106 64-65 -0.3587112046024359 1106 66-67 -0.31257287321874827 1106 68-69 -0.367801773191502 1106 70-71 -0.5905117481216422 1106 72-73 -0.29203209904322946 1106 74-75 -0.49606808534212377 1106 76-77 -0.23165995133134842 1106 78-79 -0.15845011514308638 1106 80-81 -0.4419880150627833 1106 82-83 -0.4803471094003058 1106 84-85 -0.44274290541659056 1106 86-87 -0.29305207929030885 1106 88-89 -0.19694376168468963 1106 90-91 -0.20837730081484906 1106 92-93 -0.31243386591584965 1106 94-95 -0.38309352981807976 1106 96-97 -0.39560558837523985 1106 98-99 -0.018122633978833846 1106 100-101 0.01891097314123158 1107 1 0.06428833094460629 1107 2 0.0791272583187208 1107 3 0.11981489893980779 1107 4 0.09076712969100953 1107 5 0.03709558393671841 1107 6 0.07419880585466387 1107 7 0.06889793308168635 1107 8 0.04668202019536949 1107 9 0.14157977477821504 1107 10-11 0.041219844132825756 1107 12-13 0.06623098604373467 1107 14-15 0.09546590874474958 1107 16-17 0.06724470614835809 1107 18-19 0.04824041675774993 1107 20-21 0.07862700527228839 1107 22-23 0.06755159709913272 1107 24-25 0.052584853917700514 1107 26-27 0.010931266321200894 1107 28-29 -0.038581897804888854 1107 30-31 0.0667724353500816 1107 32-33 0.08698742584014951 1107 34-35 0.07255293742894509 1107 36-37 0.29497683168371935 1107 38-39 0.22089440368184654 1107 40-41 0.22491837981727514 1107 42-43 0.21567053839267913 1107 44-45 0.2923810423316411 1107 46-47 0.3069867990344548 1107 48-49 0.33730414986161605 1107 50-51 0.2866657178750529 1107 52-53 0.2297824656266485 1107 54-55 0.20060173176086948 1107 56-57 0.376884152071014 1107 58-59 0.24904074271098153 1107 60-61 0.3204324745964229 1107 62-63 0.32295498717065385 1107 64-65 0.2479586035009973 1107 66-67 0.34679221429532703 1107 68-69 0.38416614748294364 1107 70-71 0.41872352593509277 1107 72-73 0.3908184064591751 1107 74-75 0.29429784925975966 1107 76-77 0.34557703890330416 1107 78-79 0.47842314071776926 1107 80-81 0.529766556827834 1107 82-83 0.47277024709898186 1107 84-85 0.6443837433793362 1107 86-87 0.6903948713769097 1107 88-89 0.5657462371785229 1107 90-91 0.4806270593038171 1107 92-93 0.5134840875786644 1107 94-95 0.4053990939172465 1107 96-97 0.5191126540825586 1107 98-99 0.610983633370882 1107 100-101 0.6339462317963722 1108 1 0.06432394030986899 1108 2 0.06262486104537857 1108 3 0.08618694050102249 1108 4 0.06650189079032032 1108 5 0.0542109709978007 1108 6 0.05384296655464027 1108 7 0.07516518136798567 1108 8 0.049805470234154825 1108 9 0.05258179688146214 1108 10-11 0.10974625229245305 1108 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62-63 -0.45824120354316733 2316 64-65 -0.5041229933118458 2316 66-67 -0.3543916242740792 2316 68-69 -0.40659490090017414 2316 70-71 -0.5920803162535719 2316 72-73 -0.43246555713970025 2316 74-75 -0.6482286920849596 2316 76-77 -0.5374019871280495 2316 78-79 -0.41874799886008773 2316 80-81 -0.47795884581642767 2316 82-83 -0.42099479893708747 2316 84-85 -0.5388135701320742 2316 86-87 -0.6484370626864937 2316 88-89 -0.7226096732060547 2316 90-91 -0.6744031492979907 2316 92-93 -0.8535019274906794 2316 94-95 -0.7109566327617394 2316 96-97 -0.8321135807205984 2316 98-99 -0.7637634287106394 2316 100-101 -0.8287560499915472 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 4.0 4 17.0 5 23.0 6 38.0 7 126.0 8 334.0 9 655.0 10 1100.0 11 1750.0 12 2703.0 13 4031.0 14 5486.0 15 7440.0 16 9740.0 17 12873.0 18 13107.0 19 9729.0 20 8799.0 21 8717.0 22 8975.0 23 9682.0 24 11042.0 25 12940.0 26 15482.0 27 18790.0 28 23185.0 29 28226.0 30 34268.0 31 42555.0 32 52853.0 33 69254.0 34 95673.0 35 143696.0 36 235540.0 37 369055.0 38 288318.0 39 27353.0 40 4.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.086975697237996 18.63461716890172 15.075948514393422 23.202458619466867 2 15.97113422243352 19.066311331673607 40.2904374166985 24.672117029194375 3 20.05187264572737 22.415567132240927 29.272836007573076 28.259724214458625 4 13.40973305218847 16.093637000291057 35.648075771845605 34.84855417567487 5 14.230171616679325 36.57510175938077 33.944196776110296 15.250529847829611 6 34.02882370361332 35.219876382687474 16.135262200632194 14.616037713067008 7 29.913203513548208 32.09372851218188 19.106411806050716 18.88665616821919 8 26.786475753371814 35.44353913644166 18.40693612378055 19.363048986405975 9 26.000550344791645 16.94566266660481 19.707364007323523 37.346422981280014 10-11 25.673025472080614 26.20643176072691 26.189050856462327 21.93149191073015 12-13 26.332546585271928 23.183520742059756 27.084056213720935 23.399876458947386 14-15 22.930941568386707 24.85475664827532 25.306723709078614 26.90757807425936 16-17 22.591702890261573 28.143636086326545 26.25927832430921 23.00538269910267 18-19 22.524698678034476 26.455547463531875 28.715033242965344 22.304720615468305 20-21 22.83386905919421 25.581195831633373 29.288603083696525 22.29633202547589 22-23 22.753732604797 25.47789542987075 29.12280133149801 22.645570633834232 24-25 22.688366665882846 25.670374143542336 29.089785934043306 22.551473256531512 26-27 22.174648173809132 26.062152782191937 28.97414220515885 22.789056838840086 28-29 22.32745390244445 26.38238649181953 28.81847905869793 22.471680547038094 30-31 22.402841698409855 26.02623595070051 28.67928641061131 22.891635940278324 32-33 22.151236686240118 25.937746256895196 28.862547344874045 23.048469711990645 34-35 22.782678779935434 26.155736397203004 28.630023880335177 22.43156094252638 36-37 22.9214992685162 25.681500132823732 28.574420056002044 22.822580542658024 38-39 22.373707551073462 26.099094054242332 28.57066129018931 22.9565371044949 40-41 22.991934656063723 25.88731018443925 28.54427517571734 22.57647998377969 42-43 22.62128338935697 25.880950247323863 28.466013703705823 23.031752659613343 44-45 22.58491028284521 26.221037623404982 28.575332445519734 22.618719648230076 46-47 23.012666770888544 26.425297699619843 27.858825114421638 22.703210415069975 48-49 22.83977920214341 26.44553835047275 27.824857679944788 22.889824767439052 50-51 22.92436415123357 26.724204768023714 27.697948481347463 22.653482599395257 52-53 23.209163136468376 26.434512438626662 27.61943255001697 22.736891874887995 54-55 22.8520761124732 26.65436340134446 27.744975425244316 22.748585060938023 56-57 22.81219853504715 26.800178702386805 27.66089887554764 22.7267238870184 58-59 22.81251628466807 26.44655514925971 27.611552359418035 23.129376206654186 60-61 22.571725621931332 26.682039393327006 27.771952368060827 22.97428261668084 62-63 22.641312788913844 26.656555873728845 27.894762596548222 22.807368740809093 64-65 22.36267814632497 26.70116792050667 27.837821864478517 23.098332068689842 66-67 22.3490466875873 26.715530203372467 27.731502841317113 23.20392026772312 68-69 22.770794494901526 26.374471381603303 27.842512469341006 23.012221654154164 70-71 22.6554208720829 26.49418581743632 27.49249157645755 23.35790173402323 72-73 22.62478980862576 26.65242512865682 27.419790463189976 23.302994599527445 74-75 22.574108744088267 27.00347490985443 27.290307492663164 23.132108853394136 76-77 22.70854860870151 26.616042797060942 27.454075647287752 23.221332946949794 78-79 22.76170812028221 26.75763202814499 27.41057572418316 23.070084127389638 80-81 22.8119761103125 26.8529569144224 27.189072463436553 23.145994511828548 82-83 22.79437278131327 26.901795031158528 27.228632291241677 23.075199896286524 84-85 22.777546695629432 26.99558853763343 27.195840902641827 23.03102386409531 86-87 22.884503010847055 26.904589941092194 27.06603908778849 23.14486796027226 88-89 22.750919091362608 27.029070931292953 26.926850813280673 23.293159164063766 90-91 22.767803238766568 26.969830232300986 26.95327543496856 23.309091093963886 92-93 22.8388277166161 26.616976838846146 27.175137419160293 23.369058025377466 94-95 22.630509301802405 26.62026886701924 27.221800674391794 23.52742115678656 96-97 22.894216986836582 26.325818658327222 27.21894957661438 23.561014778221814 98-99 22.583101058360437 26.535556818080714 26.939702560934847 23.941639562624 100-101 22.753478405100264 26.30572851726588 27.182367946435036 23.75842513119882 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 306.0 1 225.5 2 216.5 3 316.5 4 279.5 5 253.0 6 524.0 7 1224.0 8 1997.5 9 2272.5 10 1899.0 11 1453.5 12 1396.5 13 1469.5 14 1571.5 15 1669.0 16 1795.0 17 1976.5 18 2104.0 19 2284.5 20 2569.5 21 2937.5 22 3454.5 23 4116.0 24 5141.5 25 6513.0 26 8289.5 27 10564.0 28 12331.5 29 13890.0 30 15946.5 31 18933.5 32 23232.0 33 25479.5 34 25995.0 35 28002.0 36 31257.0 37 34154.5 38 36261.5 39 38629.0 40 41080.5 41 44157.0 42 46253.0 43 48090.5 44 50913.0 45 54049.0 46 56063.0 47 58167.0 48 59453.0 49 59083.5 50 59091.0 51 58532.5 52 58561.0 53 59681.0 54 60718.5 55 57358.5 56 52376.0 57 48296.5 58 44737.0 59 40166.0 60 32635.5 61 26694.0 62 22279.0 63 16614.0 64 11789.0 65 8873.5 66 6503.5 67 4853.5 68 3683.0 69 2874.0 70 2187.5 71 1473.0 72 1009.0 73 797.0 74 606.5 75 391.5 76 277.0 77 179.5 78 106.0 79 61.0 80 32.0 81 19.5 82 8.5 83 5.0 84 4.0 85 1.5 86 1.0 87 0.5 88 0.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.013663233699762195 3 0.006164342645939224 4 0.0 5 6.354992418494045E-5 6 0.0 7 0.0 8 4.4484946929458316E-4 9 4.4484946929458316E-4 10-11 0.0 12-13 0.0 14-15 0.0 16-17 3.812995451096427E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.005020444010610295 26-27 0.021988273767989393 28-29 6.354992418494045E-5 30-31 0.0 32-33 3.812995451096427E-4 34-35 0.019382726876406835 36-37 0.0033045960576169034 38-39 0.01862012778618755 40-41 0.014584707600443834 42-43 0.007816640674747674 44-45 0.002383122156935267 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 4.130745072021129E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.003272821095524433 86-87 3.4952458301717245E-4 88-89 6.354992418494045E-5 90-91 2.541996967397618E-4 92-93 2.85974658832232E-4 94-95 0.0 96-97 3.1774962092470224E-5 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1573566.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.15324577416994 #Duplication Level Percentage of deduplicated Percentage of total 1 78.59877258983147 46.49372512554392 2 12.560914908464257 14.86037773457643 3 3.8109746831561284 6.762945662156216 4 1.6377083104606929 3.8750304878032784 5 0.8738576873196636 2.5845759274833906 6 0.5520805746071595 1.9594414750129363 7 0.36367891648837175 1.5058951830944334 8 0.2594580002283474 1.2278226284465659 9 0.20840597669037997 1.1095100963974778 >10 1.0409088793001218 11.083698759052652 >50 0.0617016982047764 2.4723753912147117 >100 0.02724712272629972 3.019309960007256 >500 0.0026816578264791764 1.0966958261915392 >1k 0.0013944620697691715 1.2175709447344043 >5k 2.145326261183341E-4 0.731024798284698 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6248 0.3970599263075079 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5273 0.335098750227191 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3303 0.20990539958285828 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 1717 0.10911521982554274 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 1627 0.10339572664889811 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 1589 0.10098082952987038 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 6.354992418494045E-5 0.0 0.0 0.0 0.0 12-13 6.354992418494045E-5 0.0 0.0 0.0 6.354992418494045E-5 14-15 6.354992418494045E-5 0.0 0.0 0.0 6.354992418494045E-5 16-17 6.354992418494045E-5 0.0 0.0 0.0 6.354992418494045E-5 18-19 6.354992418494045E-5 0.0 0.0 0.0 6.354992418494045E-5 20-21 6.354992418494045E-5 6.354992418494045E-5 0.0 9.532488627741067E-5 6.354992418494045E-5 22-23 6.354992418494045E-5 6.354992418494045E-5 0.0 2.2242473464729155E-4 6.354992418494045E-5 24-25 1.270998483698809E-4 6.354992418494045E-5 0.0 4.766244313870534E-4 6.354992418494045E-5 26-27 1.270998483698809E-4 6.354992418494045E-5 0.0 8.57923976496696E-4 6.354992418494045E-5 28-29 1.5887481046235113E-4 6.354992418494045E-5 0.0 0.0024466720811202073 6.354992418494045E-5 30-31 2.541996967397618E-4 6.354992418494045E-5 0.0 0.010581062376792585 6.354992418494045E-5 32-33 2.541996967397618E-4 6.354992418494045E-5 0.0 0.02573771929490088 6.354992418494045E-5 34-35 2.541996967397618E-4 6.354992418494045E-5 0.0 0.046073695034081825 6.354992418494045E-5 36-37 2.541996967397618E-4 6.354992418494045E-5 0.0 0.08210650204694306 6.354992418494045E-5 38-39 2.541996967397618E-4 6.354992418494045E-5 0.0 0.14006403290360875 6.354992418494045E-5 40-41 2.541996967397618E-4 6.354992418494045E-5 0.0 0.20119905996952145 6.354992418494045E-5 42-43 3.1774962092470226E-4 6.354992418494045E-5 0.0 0.2614126131347525 6.354992418494045E-5 44-45 3.812995451096427E-4 6.354992418494045E-5 0.0 0.3357977993932253 6.354992418494045E-5 46-47 5.083993934795236E-4 6.354992418494045E-5 0.0 0.4269601656365224 6.354992418494045E-5 48-49 5.71949317664464E-4 6.354992418494045E-5 0.0 0.524445749336221 6.354992418494045E-5 50-51 5.71949317664464E-4 6.354992418494045E-5 0.0 0.6279368008713966 1.270998483698809E-4 52-53 5.71949317664464E-4 6.354992418494045E-5 0.0 0.7225626379827729 1.5887481046235113E-4 54-55 5.71949317664464E-4 6.354992418494045E-5 0.0 0.8208425957347834 1.9064977255482134E-4 56-57 5.71949317664464E-4 6.354992418494045E-5 0.0 0.9378697811213511 2.2242473464729155E-4 58-59 5.71949317664464E-4 6.354992418494045E-5 0.0 1.0651920542258795 2.541996967397618E-4 60-61 5.71949317664464E-4 6.354992418494045E-5 0.0 1.2002991930430627 2.541996967397618E-4 62-63 5.71949317664464E-4 6.354992418494045E-5 0.0 1.3305447626600981 2.85974658832232E-4 64-65 5.71949317664464E-4 6.354992418494045E-5 0.0 1.461966005874555 3.1774962092470226E-4 66-67 5.71949317664464E-4 6.354992418494045E-5 0.0 1.6170913708099945 3.1774962092470226E-4 68-69 5.71949317664464E-4 6.354992418494045E-5 0.0 1.7931564357643721 3.1774962092470226E-4 70-71 6.037242797569343E-4 6.354992418494045E-5 0.0 1.9760213426065385 3.1774962092470226E-4 72-73 6.354992418494045E-4 6.354992418494045E-5 0.0 2.1622226204684134 3.4952458301717245E-4 74-75 6.354992418494045E-4 6.354992418494045E-5 0.0 2.3494406971172483 3.812995451096427E-4 76-77 6.354992418494045E-4 9.532488627741067E-5 0.0 2.5468585365977656 4.4484946929458316E-4 78-79 6.354992418494045E-4 1.270998483698809E-4 0.0 2.768711321927393 4.4484946929458316E-4 80-81 7.943740523117557E-4 1.270998483698809E-4 0.0 3.002130193458679 4.766244313870534E-4 82-83 8.896989385891663E-4 1.270998483698809E-4 0.0 3.2503561973250568 6.354992418494045E-4 84-85 8.896989385891663E-4 1.5887481046235113E-4 0.0 3.48965343684345 6.354992418494045E-4 86-87 9.214739006816365E-4 1.9064977255482134E-4 0.0 3.7334944959410663 6.354992418494045E-4 88-89 9.532488627741067E-4 1.9064977255482134E-4 0.0 4.0043124978551905 6.354992418494045E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3000 0.0 55.899895 1 GTATCAA 5085 0.0 39.986217 1 TGGTATC 1275 0.0 32.78914 2 GTGGTAT 1425 0.0 32.671474 1 ATCAACG 6530 0.0 30.841904 3 TCAACGC 6530 0.0 30.839945 4 TATCAAC 6565 0.0 30.682354 2 CAACGCA 6695 0.0 30.008944 5 AACGCAG 6985 0.0 28.89904 6 CGCAGAG 8035 0.0 24.767893 8 ACGCAGA 8110 0.0 24.538845 7 GCAGAGT 9045 0.0 22.002213 9 GAGTACT 5950 0.0 21.953524 12-13 GTACTTT 6120 0.0 21.382511 14-15 TAAGGTG 875 0.0 21.169836 5 GTAAGGT 905 0.0 20.992895 4 CAGAGTA 8525 0.0 20.75423 10-11 AGAGTAC 8095 0.0 20.536478 10-11 TACATGG 4790 0.0 19.835905 2 AGTACTT 6070 0.0 19.758831 12-13 >>END_MODULE