FastQCFastQC Report
Thu 26 May 2016
SRR937944_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937944_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1571887
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59670.37960743997501095No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48690.3097550905376786No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30460.19377983277423888No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA17010.10821388560373615No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA16310.10376063928259475No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA15810.10057974905320802No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA30100.060.1076471
GTATCAA52650.042.502271
TATCAAC64850.034.171342
ATCAACG64600.034.0102123
TCAACGC65250.033.5985344
CAACGCA65700.033.2960245
AACGCAG68150.032.1626556
CGCAGAG78550.027.5332458
ACGCAGA79050.027.4218487
GCAGAGT86000.024.9822869
GTGTAAG7250.024.9550361
TATACCG1000.001642729323.7777045
GTACATG47300.023.4534871
GAGTACT59100.023.44537412-13
CAGAGTA82000.023.27324710-11
AGGACCG5650.022.7255945
TACATGG46000.022.536412
ATACCGT1903.2577918E-722.5219366
GTACTTT60800.022.24007614-15
GTGGTAT13100.022.170281
Produced by FastQC (version 0.11.5)