##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937944_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1571887 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.011187827114796 33.0 31.0 34.0 30.0 34.0 2 32.14192432407673 34.0 31.0 34.0 30.0 34.0 3 32.167564207859726 34.0 31.0 34.0 30.0 34.0 4 35.669706537429214 37.0 35.0 37.0 35.0 37.0 5 35.669589480668776 37.0 35.0 37.0 35.0 37.0 6 35.70006304524435 37.0 36.0 37.0 35.0 37.0 7 35.631624919603 37.0 35.0 37.0 33.0 37.0 8 35.62428024406334 37.0 35.0 37.0 33.0 37.0 9 37.26987563355381 39.0 37.0 39.0 34.0 39.0 10-11 37.18566665415516 39.0 37.0 39.0 34.0 39.0 12-13 37.03363823226479 39.0 37.0 39.0 33.0 39.0 14-15 38.24005510574233 40.0 38.0 41.0 33.5 41.0 16-17 38.05967159216915 40.0 38.0 41.0 33.0 41.0 18-19 38.22793018836596 40.0 38.0 41.0 33.5 41.0 20-21 38.22248450429325 40.0 38.0 41.0 34.0 41.0 22-23 38.11437145290978 40.0 38.0 41.0 33.0 41.0 24-25 37.992934924711506 40.0 38.0 41.0 33.0 41.0 26-27 37.817121714219915 40.0 38.0 41.0 33.0 41.0 28-29 37.63576071307925 40.0 38.0 41.0 32.0 41.0 30-31 37.41623602714444 40.0 37.0 41.0 32.0 41.0 32-33 37.13636699075697 40.0 36.5 41.0 31.0 41.0 34-35 36.90353218774632 40.0 36.0 41.0 30.5 41.0 36-37 36.64369321713329 39.0 36.0 41.0 30.0 41.0 38-39 36.340364161037016 39.0 35.0 41.0 30.0 41.0 40-41 36.241140425488595 39.0 35.0 41.0 30.0 41.0 42-43 36.01783811431738 39.0 35.0 41.0 29.0 41.0 44-45 35.688757207102036 39.0 35.0 40.5 27.5 41.0 46-47 35.324510286044735 39.0 35.0 40.0 26.5 41.0 48-49 34.91896491287224 38.0 34.5 40.0 24.5 41.0 50-51 34.1887511634106 37.5 33.5 39.5 23.5 40.5 52-53 34.253175323671485 38.0 34.0 40.0 24.0 40.5 54-55 34.328276778165346 38.0 34.0 40.0 23.0 41.0 56-57 33.99969177173677 38.0 33.5 40.0 22.5 41.0 58-59 33.57225614818368 37.5 33.0 40.0 20.0 41.0 60-61 33.06902118282039 37.0 32.5 40.0 17.5 41.0 62-63 32.51584624085574 36.0 32.0 39.0 13.0 41.0 64-65 31.9698823770411 36.0 31.0 39.0 8.0 40.5 66-67 31.394918018916115 35.0 30.5 39.0 4.5 40.0 68-69 30.701422239639363 35.0 29.5 38.0 2.0 40.0 70-71 30.112436199294223 35.0 29.0 37.0 2.0 39.5 72-73 29.423790005261193 34.0 28.5 36.5 2.0 39.0 74-75 28.73737584190212 34.0 27.5 36.0 2.0 39.0 76-77 27.99387996719866 33.5 26.0 35.5 2.0 38.0 78-79 27.242853016788104 33.0 25.5 35.0 2.0 37.0 80-81 26.532048741417164 32.5 23.5 35.0 2.0 36.5 82-83 25.79665459412795 32.0 21.0 35.0 2.0 36.0 84-85 25.075500656217656 31.5 18.5 35.0 2.0 35.5 86-87 24.35025164022605 31.0 12.0 34.0 2.0 35.0 88-89 23.481062570019347 30.5 4.5 34.0 2.0 35.0 90-91 22.59391546593362 30.0 2.0 34.0 2.0 35.0 92-93 21.66313131923605 29.0 2.0 34.0 2.0 35.0 94-95 20.594289220535572 28.5 2.0 34.0 2.0 35.0 96-97 19.347657624243983 26.5 2.0 33.5 2.0 35.0 98-99 17.797003855875136 24.0 2.0 33.0 2.0 35.0 100-101 13.847391701820804 2.0 2.0 28.5 2.0 32.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.1630451037075531 1101 2 0.16126501877688781 1101 3 0.13337410460041355 1101 4 0.026674502367264097 1101 5 0.037210745763886166 1101 6 0.07490434688584457 1101 7 0.04442127204285384 1101 8 0.192758046139609 1101 9 0.23160155291533613 1101 10-11 0.2543336017281632 1101 12-13 0.23368955536667357 1101 14-15 0.23360932952058988 1101 16-17 0.35441932972837975 1101 18-19 0.29111000434824774 1101 20-21 0.275179802484125 1101 22-23 0.21394948171771233 1101 24-25 0.18353901634721836 1101 26-27 0.31604203783471263 1101 28-29 0.35456015402013463 1101 30-31 0.3075267409546498 1101 32-33 0.20114367468418237 1101 34-35 0.13328189585003258 1101 36-37 -0.031477655920610914 1101 38-39 -0.06881683250297499 1101 40-41 0.1071764392621617 1101 42-43 0.2184400579066974 1101 44-45 0.3516403577548459 1101 46-47 0.4062044981356294 1101 48-49 0.6569546202092909 1101 50-51 0.4825886372756685 1101 52-53 0.5470696532437458 1101 54-55 0.5041618626920226 1101 56-57 0.23839863435120634 1101 58-59 0.3746183645612362 1101 60-61 0.33038756277350956 1101 62-63 0.17256686740561378 1101 64-65 0.10865538618966397 1101 66-67 -0.008510898459689997 1101 68-69 0.13656522221658562 1101 70-71 0.013837130094287886 1101 72-73 -0.22544152746491264 1101 74-75 -0.5052838237188588 1101 76-77 -0.19189070076158288 1101 78-79 -0.6384427131103827 1101 80-81 -0.6433011830119852 1101 82-83 -0.4542095827813597 1101 84-85 -0.8408860613859765 1101 86-87 -0.7416645555754471 1101 88-89 -0.7282001103820228 1101 90-91 -0.8202608384124979 1101 92-93 -0.707645588900391 1101 94-95 -1.1088214147903273 1101 96-97 -1.1233645606740268 1101 98-99 -1.088124865780319 1101 100-101 -0.8071365486476125 1102 1 0.2453940733959996 1102 2 0.22580448474964498 1102 3 0.334903513607145 1102 4 0.26983646875027034 1102 5 0.2652399086210977 1102 6 0.25332945882527014 1102 7 0.3129127271381478 1102 8 0.2829641084505994 1102 9 0.3049161319996543 1102 10-11 0.31304166839952074 1102 12-13 0.4565903688322237 1102 14-15 0.5508848059957288 1102 16-17 0.5673520254172502 1102 18-19 0.5773253968981251 1102 20-21 0.4554634605003969 1102 22-23 0.48682568023144057 1102 24-25 0.5470943531032049 1102 26-27 0.4008677405329877 1102 28-29 0.4077600977573894 1102 30-31 0.4727411254636493 1102 32-33 0.37436471695157536 1102 34-35 0.39399783930596755 1102 36-37 0.4183394901515385 1102 38-39 0.459879747190854 1102 40-41 0.46125126871570643 1102 42-43 0.5358229024574115 1102 44-45 0.5176576585495596 1102 46-47 0.5688352504154395 1102 48-49 0.46467855766387345 1102 50-51 0.28738527088797383 1102 52-53 0.5170387087328763 1102 54-55 0.471607533976929 1102 56-57 0.5621075215209572 1102 58-59 0.4834745897997621 1102 60-61 0.5864934773947894 1102 62-63 0.5655312108896808 1102 64-65 0.5593077996270814 1102 66-67 0.22743959032292693 1102 68-69 0.2184443979673425 1102 70-71 -0.04393345855470088 1102 72-73 0.11166445748480314 1102 74-75 0.12091750314425909 1102 76-77 -0.22854775587563125 1102 78-79 -0.11670184994607169 1102 80-81 -0.24288366654340976 1102 82-83 -0.22030658913436696 1102 84-85 -0.006774426718749282 1102 86-87 -0.06732200921728548 1102 88-89 -0.05344450168943027 1102 90-91 -0.3627816439074927 1102 92-93 -0.465972944316853 1102 94-95 -0.5236792341400225 1102 96-97 -0.7549408553032819 1102 98-99 -0.8122289987479441 1102 100-101 -1.1458764975422842 1103 1 0.12439080919785539 1103 2 0.18659013573844163 1103 3 0.1534939633037169 1103 4 0.044820820790114624 1103 5 0.1534912630950629 1103 6 0.08737627931680692 1103 7 0.1425206868460549 1103 8 0.06259775549094826 1103 9 0.1672010706977929 1103 10-11 0.05021292102154007 1103 12-13 -0.002449665267988621 1103 14-15 0.10957149906015218 1103 16-17 0.029238978720151465 1103 18-19 0.11648029487790978 1103 20-21 0.1436089248353838 1103 22-23 0.10314151103155922 1103 24-25 -0.007328691462632264 1103 26-27 0.08651065296049154 1103 28-29 -0.013452703283569178 1103 30-31 -0.043076214046614325 1103 32-33 0.09258499984874646 1103 34-35 -0.037953388413910716 1103 36-37 -0.27462061035434715 1103 38-39 -0.07365983261374254 1103 40-41 0.010835223667527316 1103 42-43 0.09402097534662346 1103 44-45 0.057040040853344465 1103 46-47 -0.08582582261959004 1103 48-49 -0.14903062583790927 1103 50-51 0.008624768807187877 1103 52-53 0.03294982383231826 1103 54-55 -0.03518513896387532 1103 56-57 0.031081935143099315 1103 58-59 0.038339366746221515 1103 60-61 -0.10405515467062543 1103 62-63 -0.3177009061278966 1103 64-65 -0.3180476390205733 1103 66-67 -0.24599342631214682 1103 68-69 -0.1754303510483517 1103 70-71 -0.35342947084872733 1103 72-73 -0.34071033079100843 1103 74-75 -0.1391172701005381 1103 76-77 -0.47593404175657383 1103 78-79 -0.5335018140511067 1103 80-81 -0.50349814343042 1103 82-83 -0.7403651427917239 1103 84-85 -0.7307522615794895 1103 86-87 -0.9881765336651185 1103 88-89 -0.9278693410033867 1103 90-91 -1.0498457725011967 1103 92-93 -1.1512690216704975 1103 94-95 -1.1057061777961508 1103 96-97 -1.1295462911466458 1103 98-99 -1.5197409169971685 1103 100-101 -1.6680117991160532 1104 1 0.2953602629103571 1104 2 0.353135212772834 1104 3 0.25413245889563285 1104 4 0.21791673572138137 1104 5 0.2704967804925289 1104 6 0.35526324296507283 1104 7 0.3452618972444128 1104 8 0.4084826822628429 1104 9 0.4529617102906869 1104 10-11 0.443056378156264 1104 12-13 0.38226943267696356 1104 14-15 0.38362652901320615 1104 16-17 0.37839246627086 1104 18-19 0.5528894172722332 1104 20-21 0.35583691268271167 1104 22-23 0.5809709775002503 1104 24-25 0.6971718537618443 1104 26-27 0.5515935682312332 1104 28-29 0.5115538977046938 1104 30-31 0.5831038197641618 1104 32-33 0.5340586661919318 1104 34-35 0.48698677405612045 1104 36-37 0.6499380235668397 1104 38-39 0.6846692000757315 1104 40-41 0.6740206548577987 1104 42-43 0.6589369799141664 1104 44-45 0.6009994079128731 1104 46-47 0.30091986444898566 1104 48-49 0.3413390037866435 1104 50-51 0.2790053200668581 1104 52-53 0.194794482861262 1104 54-55 0.1457728101690705 1104 56-57 0.36550064525855674 1104 58-59 0.2788230134407641 1104 60-61 0.21129720093918536 1104 62-63 0.5119115129885685 1104 64-65 0.35773539249614217 1104 66-67 0.4727322486174721 1104 68-69 0.2997360024285669 1104 70-71 0.22385502831629012 1104 72-73 0.15093577565464855 1104 74-75 0.18336415283249963 1104 76-77 0.09521003809995676 1104 78-79 0.05490063534417189 1104 80-81 -0.07843714858433515 1104 82-83 -0.039396340605339475 1104 84-85 -0.06565297242876866 1104 86-87 0.055575373763304015 1104 88-89 0.012920651555500484 1104 90-91 -0.21794893994683662 1104 92-93 -0.5288828415681053 1104 94-95 -0.48088899088016746 1104 96-97 -0.6280066320662563 1104 98-99 -0.9828173890175478 1104 100-101 -1.2284417034788788 1105 1 0.16506713542817408 1105 2 0.20919173416890402 1105 3 0.2760151026977411 1105 4 0.2594398413735206 1105 5 0.21576386973268313 1105 6 0.2295300954939421 1105 7 0.3181880433215838 1105 8 0.21538215886787526 1105 9 0.28514326785098376 1105 10-11 0.24268848935796683 1105 12-13 0.2605850345811689 1105 14-15 0.27431851502093707 1105 16-17 0.34255700691944213 1105 18-19 0.4051766401227681 1105 20-21 0.23926153328711308 1105 22-23 0.31524282726748964 1105 24-25 0.29438786596563205 1105 26-27 0.27692792292995705 1105 28-29 0.30054057611925344 1105 30-31 0.26151016099663593 1105 32-33 0.2499607334705516 1105 34-35 0.22530408554188597 1105 36-37 0.2053383286613908 1105 38-39 0.13065299850291012 1105 40-41 0.09122266164352766 1105 42-43 0.15848163944442462 1105 44-45 0.1373115775033611 1105 46-47 0.27078848968240976 1105 48-49 0.18316819028883913 1105 50-51 0.1502162955803925 1105 52-53 0.06885256918198479 1105 54-55 0.3208454673848209 1105 56-57 0.24156420224021957 1105 58-59 0.037540235502255825 1105 60-61 0.06491428097390184 1105 62-63 0.22073361130265567 1105 64-65 0.20700783404160106 1105 66-67 0.03135445739683007 1105 68-69 0.14935036028343873 1105 70-71 -0.1659723039474592 1105 72-73 -0.23422472775136782 1105 74-75 -0.31425887128575525 1105 76-77 -0.2822326909182209 1105 78-79 -0.38772482164012345 1105 80-81 -0.32763824797677543 1105 82-83 -0.28051802335960474 1105 84-85 -0.2600965036352072 1105 86-87 -0.3231741988413077 1105 88-89 -0.324583622398368 1105 90-91 -0.441013167610496 1105 92-93 -0.6350758007222552 1105 94-95 -0.5365408017215465 1105 96-97 -0.7164363814152992 1105 98-99 -0.5337744259182067 1105 100-101 -0.32684784745194584 1106 1 0.19069225293146985 1106 2 0.030261484121901105 1106 3 0.14666748096433224 1106 4 0.020244503904066846 1106 5 0.1449009255702478 1106 6 -0.033575751469776094 1106 7 0.08303199895233604 1106 8 -0.09000513197626958 1106 9 0.09693119791302962 1106 10-11 0.10264618817353721 1106 12-13 0.14595634497786136 1106 14-15 0.06391054359774273 1106 16-17 -0.3251150178972537 1106 18-19 0.051299031549724816 1106 20-21 0.1677170700421371 1106 22-23 0.14589661557356237 1106 24-25 -0.021976582238920628 1106 26-27 0.033083685291302345 1106 28-29 0.009819105918083437 1106 30-31 -0.07577739305487086 1106 32-33 -0.1627413721884139 1106 34-35 -0.38651063527029805 1106 36-37 -0.31025435793382883 1106 38-39 -0.26988798664467595 1106 40-41 -0.2620443998341955 1106 42-43 -0.5857339085909246 1106 44-45 -0.4251169804962629 1106 46-47 -0.18818923169379076 1106 48-49 -0.30183744986908323 1106 50-51 -0.36892358033612993 1106 52-53 -0.6508771732507981 1106 54-55 -0.7101627108441662 1106 56-57 -0.78992009932022 1106 58-59 -0.7602102815122223 1106 60-61 -0.3262560517954114 1106 62-63 -0.5480908857832603 1106 64-65 -0.5508051947231998 1106 66-67 -0.8135961912213325 1106 68-69 -1.2857615028487324 1106 70-71 -1.1299096041918624 1106 72-73 -0.934399572647024 1106 74-75 -0.8233197849913374 1106 76-77 -0.9537517734525309 1106 78-79 -0.8316484915028788 1106 80-81 -1.0370079603287223 1106 82-83 -1.172949401438192 1106 84-85 -1.2120663287903106 1106 86-87 -1.1089290399768572 1106 88-89 -1.3703685443761842 1106 90-91 -1.2477973375672526 1106 92-93 -1.020668487317483 1106 94-95 -1.4541249340772495 1106 96-97 -1.7192597504696323 1106 98-99 -1.9412246045232102 1106 100-101 -1.6032776274857863 1107 1 0.08694713052830849 1107 2 0.09898731413083794 1107 3 -0.0056988347333586375 1107 4 0.08705417408042848 1107 5 0.09829704093222347 1107 6 0.040701492224776814 1107 7 -0.021067580320874413 1107 8 0.01741448304782267 1107 9 -0.0042132661507992 1107 10-11 0.16393321976073594 1107 12-13 0.038361899443451364 1107 14-15 -0.005237539152460613 1107 16-17 -0.0595836595818966 1107 18-19 0.04640072535654127 1107 20-21 0.03617215255255246 1107 22-23 0.10193785987848258 1107 24-25 0.07893920700529122 1107 26-27 0.1820044011808193 1107 28-29 0.0718865530002546 1107 30-31 -0.020254754465725 1107 32-33 0.040405359004232366 1107 34-35 0.07495493271086673 1107 36-37 -0.05367096465238319 1107 38-39 0.07491571051923529 1107 40-41 -0.01065403949151289 1107 42-43 -0.06508690490683477 1107 44-45 -0.08018553934352468 1107 46-47 -0.14653989548293822 1107 48-49 -0.25019399947342436 1107 50-51 -0.25951150787521016 1107 52-53 -0.16369931263562165 1107 54-55 -0.257106664804013 1107 56-57 -0.3347769868139565 1107 58-59 -0.5225133791949546 1107 60-61 -0.5317762924598597 1107 62-63 -0.4057962898335745 1107 64-65 -0.6172540862280087 1107 66-67 -0.6903557229720327 1107 68-69 -0.4503873175981461 1107 70-71 -0.5116147466503911 1107 72-73 -0.5733231224475581 1107 74-75 -0.47372002042394357 1107 76-77 -0.6090224119544843 1107 78-79 -0.6870991923508747 1107 80-81 -0.8705178681176164 1107 82-83 -0.8083174828804367 1107 84-85 -0.853030948501253 1107 86-87 -0.7493916214517995 1107 88-89 -0.812409396293436 1107 90-91 -0.8512466432590564 1107 92-93 -1.2668043406516851 1107 94-95 -1.19605460948544 1107 96-97 -1.1914468757939005 1107 98-99 -1.1658898760336847 1107 100-101 -1.3363480610741725 1108 1 -0.04534963007303361 1108 2 -0.005648865480040399 1108 3 0.05848818016820445 1108 4 0.13851380123878698 1108 5 0.09417662650482583 1108 6 0.049587973002360286 1108 7 0.09102178780661063 1108 8 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>>Per sequence quality scores pass #Quality Count 2 5526.0 3 1640.0 4 1528.0 5 1607.0 6 1872.0 7 2339.0 8 2862.0 9 3705.0 10 4531.0 11 5711.0 12 7210.0 13 8811.0 14 10435.0 15 12725.0 16 14924.0 17 18365.0 18 18441.0 19 16133.0 20 16645.0 21 17773.0 22 19866.0 23 21914.0 24 24476.0 25 27100.0 26 30880.0 27 34804.0 28 40353.0 29 46434.0 30 54463.0 31 65324.0 32 79189.0 33 98331.0 34 126973.0 35 167198.0 36 213537.0 37 226398.0 38 114659.0 39 7205.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.870823057354144 18.157249080816182 14.662025141453327 23.309902720376353 2 15.687693536632327 18.654687683989092 40.84896743333429 24.808651346044293 3 19.865534396281042 22.388102495616362 29.039638808465522 28.706724299637077 4 13.127128809242766 15.701096868265605 35.869866412541974 35.30190790994966 5 14.283028008311978 36.52069708443288 33.90350709450415 15.292767812750984 6 34.1619840189707 34.893592608056814 16.33437132466605 14.610052048306438 7 30.06548234283537 31.949278585438655 19.12121370464634 18.864025367079638 8 26.841864018334604 35.20620066208301 18.498726763432646 19.453208556149733 9 26.051037326690228 17.04265552575275 19.723889119965044 37.18241802759198 10-11 25.698386638662846 26.097651219987494 26.259152931166902 21.944809210182758 12-13 26.389545500303917 23.194623021511227 26.988937920594125 23.426893557590734 14-15 23.012780471676265 24.848673265169545 25.262096343918056 26.876449919236133 16-17 22.711233509923986 28.015228077149473 26.218106020645564 23.055432392280974 18-19 22.609037155382964 26.41637140349067 28.58373689152534 22.390854549601027 20-21 22.982392659180515 25.609415044373506 29.155632482514655 22.252559813931324 22-23 22.83914555427964 25.44364139235782 28.916568104479424 22.800644948883118 24-25 22.706399864582142 25.627957284718917 28.956788378036425 22.70885447266252 26-27 22.22370178616995 26.023020245213303 28.828761958584458 22.924516010032292 28-29 22.366582572949255 26.379448561366807 28.68883551593741 22.565133349746525 30-31 22.524706920739803 25.925852809860988 28.644712601150474 22.904727668248732 32-33 22.206294279794957 25.97005893621799 28.68797245715141 23.135674326835645 34-35 22.937034972222108 26.076864958591848 28.43988821981102 22.546211849375016 36-37 22.991295710256036 25.72152640258773 28.415462689988473 22.871715197167763 38-39 22.49970641224304 26.105600572344954 28.37537605488707 23.01931696052494 40-41 23.07410023148067 25.899131971354066 28.368874459053572 22.657893338111688 42-43 22.68358963716849 25.909179450029985 28.25315324376385 23.154077669037672 44-45 22.68265005829731 26.22965322009908 28.38958132856772 22.698115393035888 46-47 23.08333712586418 26.431436399575958 27.690164126944016 22.795062347615847 48-49 22.89610655763789 26.42288789630386 27.60911045400794 23.07189509205031 50-51 22.9771124771451 26.79359855892785 27.507419389231103 22.721869574695944 52-53 23.172350904299204 26.498290926526863 27.501430989099028 22.827927180074905 54-55 22.880327822940984 26.610217606607378 27.661620070626036 22.8478344998256 56-57 22.871355268512442 26.840267050427833 27.47852559095391 22.809852090105814 58-59 22.95746951253472 26.40996272348643 27.45111455598049 23.181453207998363 60-61 22.600161393585243 26.67480847239953 27.69561017085064 23.02941996316459 62-63 22.77621761585475 26.6166453010766 27.799062035743976 22.808075047324675 64-65 22.48723937781625 26.655507751839313 27.695355260813116 23.161897609531326 66-67 22.52323836029506 26.661641009616105 27.605813257488126 23.20930737260071 68-69 22.896109881771164 26.326191137039007 27.73249803291462 23.045200948275205 70-71 22.708425161391958 26.44253459763 27.403009679539302 23.44603056143874 72-73 22.761972200784488 26.54377938848091 27.369799581500075 23.324448829234527 74-75 22.69542748220349 26.854830754151987 27.27071380156374 23.179027962080784 76-77 22.880977379020713 26.534663230568544 27.265311316852785 23.31904807355795 78-79 22.913223889885444 26.71500394202581 27.214359027875435 23.157413140213308 80-81 23.061413380499257 26.736626484633693 26.965265402184635 23.236694732682416 82-83 23.068805020360855 26.76920218349045 27.010656240246313 23.15133655590239 84-85 23.050871184343023 26.850898863714097 26.98662322808279 23.111606723860096 86-87 23.16975239510899 26.804603903442235 26.806099255759968 23.219544445688804 88-89 23.135604630443936 26.79709166513089 26.67477048465988 23.392533219765294 90-91 23.089217357466506 26.809470569185017 26.63872238836008 23.462589684988394 92-93 23.27576382110793 26.44944076331494 26.78141094330084 23.493384472276286 94-95 23.07802498044672 26.358455435874955 26.901649796891018 23.6618697867873 96-97 23.353896857285367 26.06101194691829 26.83840673379282 23.74668446200353 98-99 23.112421500744095 26.26467663971626 26.50735120945823 24.11555065008141 100-101 24.0674137548827 25.159953829336718 26.50570635763918 24.2669260581414 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 253.0 1 200.0 2 231.5 3 310.0 4 246.0 5 244.5 6 482.5 7 1094.5 8 1808.0 9 2040.5 10 1714.5 11 1333.5 12 1311.0 13 1424.0 14 1507.0 15 1634.0 16 1802.0 17 1915.0 18 2056.0 19 2287.0 20 2597.0 21 3021.0 22 3549.0 23 4212.0 24 5268.5 25 6670.5 26 8470.0 27 10480.0 28 12175.0 29 14022.5 30 15893.5 31 18656.5 32 22774.5 33 24944.5 34 25841.0 35 27729.0 36 30802.5 37 34113.5 38 36120.5 39 38101.5 40 40811.0 41 43795.5 42 45815.0 43 47734.0 44 50335.5 45 53007.5 46 54804.0 47 56955.0 48 58663.5 49 58636.5 50 58560.5 51 57994.0 52 57809.5 53 58692.5 54 59433.5 55 56798.5 56 52277.0 57 48110.0 58 44773.0 59 40734.0 60 33710.5 61 27587.0 62 23089.0 63 17918.5 64 13268.0 65 10171.5 66 7794.5 67 5879.0 68 4474.5 69 3439.0 70 2663.0 71 1948.5 72 1367.5 73 1053.5 74 808.5 75 590.0 76 414.0 77 276.0 78 174.0 79 107.5 80 61.5 81 40.5 82 27.5 83 18.0 84 13.0 85 7.5 86 7.5 87 4.5 88 2.0 89 5.0 90 6.0 91 3.5 92 1.5 93 1.5 94 1.0 95 0.0 96 1.0 97 1.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1455575368967362 2 0.051021479279362965 3 0.15363699807937847 4 0.13067097062320637 5 0.1951158066705813 6 0.2005233200605387 7 0.16597885216939895 8 0.06915255358686725 9 0.12329130529102918 10-11 0.10337893245506835 12-13 0.1514739927233955 14-15 0.1391639475356689 16-17 0.13709636888656754 18-19 0.1336610074388299 20-21 0.13639657303610248 22-23 0.1959746470325157 24-25 0.2168412869372926 26-27 0.14985173870640828 28-29 0.12796721392822766 30-31 0.10038889563944481 32-33 0.12529526613554282 34-35 0.09437701310590392 36-37 0.0888740730090649 38-39 0.051307759400007764 40-41 0.058114864490895334 42-43 0.061391181427163655 44-45 0.04014283469486038 46-47 0.05194393744588511 48-49 0.0433237249242471 50-51 0.05308905792846433 52-53 0.026814904633730034 54-55 0.05057615464724881 56-57 0.0269421402429055 58-59 0.05028987452660401 60-61 0.03645300202877179 62-63 0.05248468878488085 64-65 0.04116071956826413 66-67 0.039220376528338236 68-69 0.06584442774830507 70-71 0.05022625672201627 72-73 0.05086243476789363 74-75 0.0414788085912028 76-77 0.02143920014606648 78-79 0.02290240965158437 80-81 0.02630596219702816 82-83 0.023315925381404642 84-85 0.020325888565781127 86-87 0.022329849410294762 88-89 0.06504920519095839 90-91 0.11059955327577617 92-93 0.08041290499889622 94-95 0.07497358270664495 96-97 0.0676575351790555 98-99 0.05426598731333741 100-101 0.13098905964614505 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1571887.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.16909810442224 #Duplication Level Percentage of deduplicated Percentage of total 1 80.25397378086365 49.09063195471378 2 11.60426479864696 14.196448237962588 3 3.5307694152591447 6.479219422382404 4 1.5407372303101998 3.7698202717592157 5 0.804322349743276 2.459983635951293 6 0.4971241308478804 1.8245180837945778 7 0.3206226282170812 1.3728537899935838 8 0.2216731109906208 1.084763541863821 9 0.17723781569360847 0.9757329602378259 >10 0.962316919014432 10.534261235429165 >50 0.057143324586827395 2.365321729198694 >100 0.02554060228768043 2.8731546880851133 >500 0.0029187565629864532 1.2245773764936492 >1k 0.001250895669851337 1.3682364667951432 >5k 1.0424130582094476E-4 0.3804766053391099 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5967 0.37960743997501095 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4869 0.3097550905376786 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3046 0.19377983277423888 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 1701 0.10821388560373615 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 1631 0.10376063928259475 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 1581 0.10057974905320802 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 6.361780458773437E-5 0.0 0.0 0.0 0.0 6 1.2723560917546873E-4 0.0 0.0 0.0 0.0 7 2.5447121835093747E-4 0.0 0.0 0.0 0.0 8 2.5447121835093747E-4 0.0 0.0 6.361780458773437E-5 0.0 9 2.5447121835093747E-4 0.0 0.0 6.361780458773437E-5 0.0 10-11 2.5447121835093747E-4 0.0 0.0 6.361780458773437E-5 0.0 12-13 2.5447121835093747E-4 0.0 0.0 6.361780458773437E-5 0.0 14-15 3.4989792523253897E-4 0.0 0.0 6.361780458773437E-5 0.0 16-17 3.8170682752640617E-4 0.0 0.0 6.361780458773437E-5 0.0 18-19 4.135157298202734E-4 0.0 0.0 6.361780458773437E-5 0.0 20-21 4.453246321141405E-4 0.0 0.0 1.2723560917546873E-4 0.0 22-23 4.453246321141405E-4 0.0 0.0 1.2723560917546873E-4 0.0 24-25 4.453246321141405E-4 0.0 0.0 3.8170682752640617E-4 0.0 26-27 4.453246321141405E-4 0.0 0.0 7.952225573466796E-4 0.0 28-29 4.7713353440800773E-4 0.0 0.0 0.0025765210858032413 0.0 30-31 5.089424367018749E-4 0.0 0.0 0.009224581665221481 0.0 32-33 5.089424367018749E-4 0.0 0.0 0.02423838354792679 0.0 34-35 5.089424367018749E-4 0.0 0.0 0.044277991993063114 0.0 36-37 5.089424367018749E-4 0.0 0.0 0.07847256195897034 0.0 38-39 5.725602412896092E-4 0.0 0.0 0.1378279736393265 0.0 40-41 5.725602412896092E-4 0.0 0.0 0.20329069456010515 0.0 42-43 5.725602412896092E-4 3.180890229386718E-5 0.0 0.2658588053721419 0.0 44-45 6.361780458773436E-4 6.361780458773437E-5 0.0 0.3416594195384274 0.0 46-47 6.997958504650779E-4 6.361780458773437E-5 0.0 0.4328555424149446 0.0 48-49 6.997958504650779E-4 6.361780458773437E-5 0.0 0.5285049116126033 0.0 50-51 6.997958504650779E-4 6.361780458773437E-5 0.0 0.6310250037057371 0.0 52-53 6.997958504650779E-4 6.361780458773437E-5 0.0 0.7272787420469793 0.0 54-55 6.997958504650779E-4 6.361780458773437E-5 0.0 0.8329797243694999 0.0 56-57 6.997958504650779E-4 6.361780458773437E-5 0.0 0.955793896126121 0.0 58-59 6.997958504650779E-4 6.361780458773437E-5 0.0 1.0821388560373615 0.0 60-61 6.997958504650779E-4 6.361780458773437E-5 0.0 1.2253107252620576 0.0 62-63 6.997958504650779E-4 6.361780458773437E-5 0.0 1.3587172614825367 0.0 64-65 6.997958504650779E-4 6.361780458773437E-5 0.0 1.4981674891388503 0.0 66-67 7.634136550528123E-4 6.361780458773437E-5 0.0 1.6549853774476155 3.180890229386718E-5 68-69 7.634136550528123E-4 6.361780458773437E-5 0.0 1.828502939460661 6.361780458773437E-5 70-71 7.634136550528123E-4 6.361780458773437E-5 0.0 2.0081596196164226 6.361780458773437E-5 72-73 7.634136550528123E-4 6.361780458773437E-5 0.0 2.1975180149718145 1.2723560917546873E-4 74-75 7.634136550528123E-4 6.361780458773437E-5 0.0 2.376506708179405 1.9085341376320309E-4 76-77 7.634136550528123E-4 6.361780458773437E-5 0.0 2.574262653740377 1.9085341376320309E-4 78-79 7.634136550528123E-4 9.542670688160156E-5 0.0 2.799851388808483 1.9085341376320309E-4 80-81 8.270314596405466E-4 1.2723560917546873E-4 0.0 3.033583202863819 1.9085341376320309E-4 82-83 8.270314596405466E-4 1.2723560917546873E-4 0.0 3.2671877813099797 1.9085341376320309E-4 84-85 8.270314596405466E-4 1.2723560917546873E-4 0.0 3.500187990612557 1.9085341376320309E-4 86-87 8.270314596405466E-4 1.2723560917546873E-4 0.0 3.7430171507239387 1.9085341376320309E-4 88-89 8.270314596405466E-4 1.2723560917546873E-4 0.0 4.007158275372212 1.9085341376320309E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3010 0.0 60.107647 1 GTATCAA 5265 0.0 42.50227 1 TATCAAC 6485 0.0 34.17134 2 ATCAACG 6460 0.0 34.010212 3 TCAACGC 6525 0.0 33.598534 4 CAACGCA 6570 0.0 33.296024 5 AACGCAG 6815 0.0 32.162655 6 CGCAGAG 7855 0.0 27.533245 8 ACGCAGA 7905 0.0 27.421848 7 GCAGAGT 8600 0.0 24.982286 9 GTGTAAG 725 0.0 24.955036 1 TATACCG 100 0.0016427293 23.777704 5 GTACATG 4730 0.0 23.453487 1 GAGTACT 5910 0.0 23.445374 12-13 CAGAGTA 8200 0.0 23.273247 10-11 AGGACCG 565 0.0 22.725594 5 TACATGG 4600 0.0 22.53641 2 ATACCGT 190 3.2577918E-7 22.521936 6 GTACTTT 6080 0.0 22.240076 14-15 GTGGTAT 1310 0.0 22.17028 1 >>END_MODULE