FastQCFastQC Report
Thu 26 May 2016
SRR937944_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937944_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1571887
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59050.3756631360905714No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50260.3197430858579529No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33420.21261070293220824No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA17120.10891368145420123No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA16860.10725961853492014No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26600.056.073851
GTATCAA47800.043.626361
ATCAACG61800.033.7380033
TATCAAC61700.033.640872
TCAACGC62350.033.3631554
CAACGCA63800.032.6049045
TATACCG600.003954247631.6619915
AACGCAG66250.031.3274486
TGGTATC11450.028.622962
ACGCAGA77200.026.7600947
CGCAGAG78100.026.2084838
GTGGTAT13500.026.0381621
GCAGAGT85900.023.3863749
GAGTACT56150.021.52620712-13
TAAGGTG9000.021.1079945
CAGAGTA81100.021.08163310-11
AGAGTAC77100.020.69700410-11
GTGTAGC10400.020.5537721
GTACTTT59250.020.23962614-15
GTACATG47350.020.0642241
Produced by FastQC (version 0.11.5)