##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937944_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1571887 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.44193189459548 33.0 31.0 34.0 31.0 34.0 2 32.66993937859401 34.0 31.0 34.0 31.0 34.0 3 32.72386437447476 34.0 31.0 34.0 31.0 34.0 4 36.1995340631992 37.0 35.0 37.0 35.0 37.0 5 36.16623523192189 37.0 35.0 37.0 35.0 37.0 6 36.196567564971275 37.0 37.0 37.0 35.0 37.0 7 36.155504816822074 37.0 36.0 37.0 35.0 37.0 8 36.136891519555796 37.0 36.0 37.0 35.0 37.0 9 37.81045202358694 39.0 38.0 39.0 35.0 39.0 10-11 37.78629602509595 39.0 38.0 39.0 35.0 39.0 12-13 37.70013556954157 39.0 37.0 39.0 35.0 39.0 14-15 38.95284489279446 40.0 38.0 41.0 35.5 41.0 16-17 38.82462193529179 40.0 38.0 41.0 35.0 41.0 18-19 39.08197694872469 40.0 38.5 41.0 36.0 41.0 20-21 39.05972248641283 40.0 39.0 41.0 35.5 41.0 22-23 38.95967744500717 40.0 38.5 41.0 35.0 41.0 24-25 38.854506398996875 40.0 38.0 41.0 35.0 41.0 26-27 38.66914002087937 40.0 38.0 41.0 34.0 41.0 28-29 38.54448888501527 40.0 38.0 41.0 34.0 41.0 30-31 38.29647582809706 40.0 38.0 41.0 34.0 41.0 32-33 38.08001179474097 40.0 37.5 41.0 33.5 41.0 34-35 37.91729271887864 40.0 37.0 41.0 33.0 41.0 36-37 37.914945858067405 40.0 37.0 41.0 33.0 41.0 38-39 37.75790626170965 40.0 37.0 41.0 33.0 41.0 40-41 37.57280103467997 40.0 37.0 41.0 33.0 41.0 42-43 37.322573760073084 40.0 37.0 41.0 32.0 41.0 44-45 37.03148954091483 40.0 36.0 41.0 31.0 41.0 46-47 36.62917340750322 39.0 36.0 41.0 30.5 41.0 48-49 36.21318644406372 39.0 35.0 41.0 30.0 41.0 50-51 35.80107316874559 39.0 35.0 40.0 28.5 41.0 52-53 35.23965208695027 38.0 34.5 40.0 27.0 41.0 54-55 34.88365575896995 38.0 34.0 40.0 26.0 41.0 56-57 34.49719381863964 38.0 34.0 40.0 26.0 41.0 58-59 34.01484330616641 37.0 33.0 40.0 24.0 41.0 60-61 33.48953582541239 37.0 33.0 39.5 22.5 41.0 62-63 32.978653681848634 36.0 32.0 39.0 21.5 40.0 64-65 32.44275574516489 35.5 31.0 39.0 19.5 40.0 66-67 31.84175929949163 35.0 31.0 38.0 17.5 40.0 68-69 31.211469399517906 35.0 30.0 38.0 12.0 40.0 70-71 30.54179244436782 34.0 29.0 37.0 8.0 39.0 72-73 29.90988188082222 34.0 29.0 36.5 7.0 39.0 74-75 29.27140150659685 34.0 28.0 36.0 2.0 38.5 76-77 27.96663850518517 31.5 26.5 34.5 2.0 36.5 78-79 28.6246263249203 33.5 28.0 35.0 2.0 37.0 80-81 28.27158790676429 33.0 27.0 35.0 2.0 37.0 82-83 27.66082358337463 33.0 26.0 35.0 2.0 36.0 84-85 27.009608197026886 32.5 25.5 35.0 2.0 36.0 86-87 26.308254028438434 32.0 24.5 35.0 2.0 35.0 88-89 25.486370521545123 31.5 21.0 34.0 2.0 35.0 90-91 24.869367200059546 31.0 19.0 34.0 2.0 35.0 92-93 23.971660494679327 31.0 11.5 34.0 2.0 35.0 94-95 23.051843739403658 30.0 2.0 34.0 2.0 35.0 96-97 21.911959320231034 29.5 2.0 34.0 2.0 35.0 98-99 20.377012151636855 29.0 2.0 34.0 2.0 35.0 100-101 16.16604278806301 13.5 2.0 30.0 2.0 33.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.10027825723075523 1101 2 0.10843903925704979 1101 3 0.1355703806264188 1101 4 0.07113354339399791 1101 5 0.1299273041798017 1101 6 0.0934991787032331 1101 7 0.07872174090435635 1101 8 0.06838756064377094 1101 9 -0.018408664723466472 1101 10-11 0.14055683718433443 1101 12-13 0.20965595792898029 1101 14-15 0.13029889902009018 1101 16-17 0.12360573971197653 1101 18-19 0.22397680472706583 1101 20-21 0.15493995893871926 1101 22-23 0.12912692181895835 1101 24-25 0.22008541969697148 1101 26-27 0.1571465402499257 1101 28-29 0.2532848905126599 1101 30-31 0.4028210178191287 1101 32-33 0.21966382442429477 1101 34-35 0.18149838098168658 1101 36-37 0.37944843416826046 1101 38-39 0.29064717990465994 1101 40-41 0.2081744864322843 1101 42-43 0.3214835957838176 1101 44-45 0.1963576939751448 1101 46-47 0.3889980768229364 1101 48-49 0.35256141958665665 1101 50-51 0.22461128266761676 1101 52-53 -0.05213482310022499 1101 54-55 0.09388181490957948 1101 56-57 0.17819706453297357 1101 58-59 0.19081416779329174 1101 60-61 0.2712254103857106 1101 62-63 0.276449511755537 1101 64-65 0.35528262803125443 1101 66-67 -0.0027184993246827105 1101 68-69 0.06536194793355321 1101 70-71 0.06783119537912441 1101 72-73 0.08375286900032819 1101 74-75 0.039112217128685955 1101 76-77 -0.34068642481669187 1101 78-79 -0.3784356065756995 1101 80-81 -0.30420550246727984 1101 82-83 -0.15778553152742347 1101 84-85 -0.2903971316919929 1101 86-87 -0.23787464353576482 1101 88-89 -0.24578331064407166 1101 90-91 -0.26615823902458047 1101 92-93 -0.5566956141682198 1101 94-95 -0.4871289822838776 1101 96-97 -0.7646202071791812 1101 98-99 -0.7844093713506055 1101 100-101 -1.0405807147498791 1102 1 0.22358369361868569 1102 2 0.18721673107785364 1102 3 0.10555701822400465 1102 4 0.152474472198179 1102 5 0.15575471820329057 1102 6 0.1676933320661007 1102 7 0.11805784048942058 1102 8 0.09394866433132165 1102 9 0.07647705157162932 1102 10-11 0.23038429809344052 1102 12-13 0.20976848342303356 1102 14-15 0.30303959808472314 1102 16-17 0.25553320765392584 1102 18-19 0.3198309435553952 1102 20-21 0.30664940873593594 1102 22-23 0.34378594612915236 1102 24-25 0.3723307721596356 1102 26-27 0.3518468239079482 1102 28-29 0.29899673147662753 1102 30-31 0.31406325238790345 1102 32-33 0.302310283387655 1102 34-35 0.33111157459586593 1102 36-37 0.14496991950478844 1102 38-39 -0.019459016030353382 1102 40-41 0.15356094117593955 1102 42-43 0.008294670167856566 1102 44-45 0.012614814729069224 1102 46-47 0.01577492470253361 1102 48-49 0.36676682549893513 1102 50-51 0.3840052859964942 1102 52-53 0.21254436202764992 1102 54-55 0.25142547714555263 1102 56-57 0.2935605453215899 1102 58-59 0.23275921784369302 1102 60-61 0.10556819117198302 1102 62-63 0.17850638401565533 1102 64-65 0.30242330834163766 1102 66-67 -0.02847464720193571 1102 68-69 0.19165563947304065 1102 70-71 0.19134175636559547 1102 72-73 0.4051885067620091 1102 74-75 0.036617667582302005 1102 76-77 -0.09913779270472745 1102 78-79 0.08653708254918158 1102 80-81 0.06802414331184181 1102 82-83 0.03103787377533962 1102 84-85 -0.39457417180264187 1102 86-87 -0.17897293924339053 1102 88-89 -0.41003021033228393 1102 90-91 -0.35967255086085714 1102 92-93 -0.23386044057420108 1102 94-95 -0.3857474974162116 1102 96-97 -0.6082728316018979 1102 98-99 -0.8526767131702364 1102 100-101 -1.3395935379676391 1103 1 0.10084670273825225 1103 2 0.09115911966325285 1103 3 0.09676230375720962 1103 4 0.12541505481829063 1103 5 0.07634788245639612 1103 6 0.02388016534521853 1103 7 0.06903070614130513 1103 8 0.10074043989806825 1103 9 0.13867315023790638 1103 10-11 0.12895702465235104 1103 12-13 0.04609194190265953 1103 14-15 0.09583929297753713 1103 16-17 0.1533541590947749 1103 18-19 0.1674377591113725 1103 20-21 0.10922105844597496 1103 22-23 0.07853092363870928 1103 24-25 0.2729739650165399 1103 26-27 0.25277605474620657 1103 28-29 0.1898563036535421 1103 30-31 0.1629901051848961 1103 32-33 0.16241078928228347 1103 34-35 0.19700911691266043 1103 36-37 0.3589521290640647 1103 38-39 0.11235850295471295 1103 40-41 0.2784619496000431 1103 42-43 0.14967483147083982 1103 44-45 0.02504277241788344 1103 46-47 -0.14169111529791678 1103 48-49 0.030120088014506052 1103 50-51 -0.012069151181712812 1103 52-53 -0.02585360093323885 1103 54-55 -0.10434273113496317 1103 56-57 -0.12182179211348654 1103 58-59 0.0186738102676145 1103 60-61 -0.17240374345432485 1103 62-63 -0.17935270582270135 1103 64-65 -0.30576365954359375 1103 66-67 -0.4839451516383271 1103 68-69 -0.2581185587793229 1103 70-71 -0.47762682432515646 1103 72-73 -0.4956500892962765 1103 74-75 -0.5352999086103658 1103 76-77 -0.5377818605013367 1103 78-79 -0.7374977355643146 1103 80-81 -0.7519446729396044 1103 82-83 -0.7868061850475314 1103 84-85 -0.5853484934439379 1103 86-87 -0.6558850751058181 1103 88-89 -1.269703790518264 1103 90-91 -1.579896221345102 1103 92-93 -1.287980621395075 1103 94-95 -1.3670110000915088 1103 96-97 -1.5209518296837636 1103 98-99 -1.4254699021011277 1103 100-101 -1.8849257346271653 1104 1 0.16278497578255724 1104 2 0.2210957158850846 1104 3 0.18971922073563974 1104 4 0.13906795599549326 1104 5 0.12274382859233413 1104 6 0.11232097762682969 1104 7 0.05114074204382035 1104 8 0.13685856158257081 1104 9 0.21936894583701871 1104 10-11 0.16403056137613703 1104 12-13 0.32352869243331384 1104 14-15 0.3808498202679118 1104 16-17 0.30345913195357355 1104 18-19 0.24742051938748233 1104 20-21 0.25128847082428507 1104 22-23 0.24232287420502274 1104 24-25 0.31080512888221534 1104 26-27 0.33838037093525486 1104 28-29 0.06438116108934366 1104 30-31 0.1352125893511058 1104 32-33 0.2093468162760317 1104 34-35 0.25279968384325713 1104 36-37 0.26926955944506403 1104 38-39 0.3375171409908049 1104 40-41 0.3755322681277278 1104 42-43 0.26789553079839834 1104 44-45 0.3599490555299809 1104 46-47 0.3915350019836481 1104 48-49 0.4000956214943727 1104 50-51 0.29636631172759564 1104 52-53 0.3391881224562141 1104 54-55 0.4690143943318219 1104 56-57 0.40221866422911745 1104 58-59 0.4276744720809802 1104 60-61 0.27097413654828273 1104 62-63 0.21654735178592688 1104 64-65 0.3150084932015673 1104 66-67 0.22128428842641412 1104 68-69 0.29700955182923394 1104 70-71 0.31355326949460505 1104 72-73 0.035837053350221026 1104 74-75 0.018652883179036905 1104 76-77 -0.10870154962050904 1104 78-79 -0.20354722411631698 1104 80-81 -0.3081295846235257 1104 82-83 -0.12197565041891778 1104 84-85 -0.07789023397880612 1104 86-87 -0.29210589788042896 1104 88-89 -0.10758180425517949 1104 90-91 -0.2986531866736257 1104 92-93 -0.2958120081512625 1104 94-95 -0.48750432537791966 1104 96-97 -0.6548959544613027 1104 98-99 -0.8097029272740386 1104 100-101 -1.2883429890961189 1105 1 0.13372866479652856 1105 2 0.1343147264318887 1105 3 0.10493712662974986 1105 4 0.013416999944887209 1105 5 0.040512956179718174 1105 6 0.09579063912968167 1105 7 0.12549438871276664 1105 8 0.07712512479763944 1105 9 0.14130024696008547 1105 10-11 0.1214866382809987 1105 12-13 0.12127368861963106 1105 14-15 0.1137145529156598 1105 16-17 0.06167663017823344 1105 18-19 0.05940764492337536 1105 20-21 0.14182922461823466 1105 22-23 0.1701160674403397 1105 24-25 0.06959632322218567 1105 26-27 0.055618870711697355 1105 28-29 0.03965469413687117 1105 30-31 0.036850828745123465 1105 32-33 0.1609590831811687 1105 34-35 0.2220272159965404 1105 36-37 0.15074295572607355 1105 38-39 0.007469272707488983 1105 40-41 0.0904135179633414 1105 42-43 0.005680241879119308 1105 44-45 0.0026941832225730877 1105 46-47 -0.15462041212960997 1105 48-49 0.058514653082177404 1105 50-51 -0.07757670660438265 1105 52-53 0.04553132752709388 1105 54-55 -0.04509951640172005 1105 56-57 -0.01955482936652686 1105 58-59 0.019362289339866834 1105 60-61 -0.11306856353851202 1105 62-63 -0.09914331915907582 1105 64-65 0.08422813497847415 1105 66-67 -0.10885234659107468 1105 68-69 -0.023190140446082808 1105 70-71 -0.17288843377464147 1105 72-73 -0.06628249900242622 1105 74-75 -0.14354859758159577 1105 76-77 -0.2608148554251919 1105 78-79 -0.40704331838001195 1105 80-81 -0.4083094194516441 1105 82-83 -0.2741563531884381 1105 84-85 -0.34042442560974706 1105 86-87 -0.5511535294524563 1105 88-89 -1.0597348907074 1105 90-91 -1.0539857660376626 1105 92-93 -0.8688423729496648 1105 94-95 -0.7663189935875963 1105 96-97 -0.7602637117533213 1105 98-99 -0.9229150337415177 1105 100-101 -1.2817676754536595 1106 1 0.08352091897964442 1106 2 0.07472457790773035 1106 3 0.10716404898357723 1106 4 0.08561948161437982 1106 5 0.13620669560442167 1106 6 0.018942547383112185 1106 7 0.013613396044974024 1106 8 0.04679235548010041 1106 9 -0.1093107701483973 1106 10-11 0.007145126718683059 1106 12-13 -0.015284875020149968 1106 14-15 0.050641606873170986 1106 16-17 0.10388851211092742 1106 18-19 0.18929108165959718 1106 20-21 0.2232909655469868 1106 22-23 0.2213691225258856 1106 24-25 0.21951219086648877 1106 26-27 0.18195981825822116 1106 28-29 0.1236859970133537 1106 30-31 0.045224583210249136 1106 32-33 0.022454665057459522 1106 34-35 0.05271860535544448 1106 36-37 -0.13464715519703674 1106 38-39 0.035437405815223144 1106 40-41 0.0029708595689115214 1106 42-43 0.08140829226453405 1106 44-45 -0.21668210931360932 1106 46-47 -0.04435062457556427 1106 48-49 -0.27637818391725233 1106 50-51 -0.3323412300110391 1106 52-53 -0.19817754632137508 1106 54-55 -0.3704012648506776 1106 56-57 -0.43651478424526147 1106 58-59 -0.34973293532642913 1106 60-61 -0.451531620504106 1106 62-63 -0.15959782188515703 1106 64-65 -0.6195352603965958 1106 66-67 -0.7879912824812827 1106 68-69 -0.8322928853229428 1106 70-71 -1.0334228387379802 1106 72-73 -1.187787011128762 1106 74-75 -0.9628487479858059 1106 76-77 -1.0077818605013356 1106 78-79 -1.057813199936696 1106 80-81 -0.9523336721307203 1106 82-83 -0.9766206321882507 1106 84-85 -1.2879075051359763 1106 86-87 -1.357886937987896 1106 88-89 -1.322440756471643 1106 90-91 -1.426653090722752 1106 92-93 -1.219108400398433 1106 94-95 -1.5906313643339764 1106 96-97 -1.7226607788712052 1106 98-99 -1.8290797263529122 1106 100-101 -1.972656940353076 1107 1 0.07280786336959011 1107 2 0.0048865468219077 1107 3 0.03406571227134947 1107 4 0.07231884725429438 1107 5 -0.048227285168579215 1107 6 0.027748598873095887 1107 7 0.05642158386788054 1107 8 0.03605139674620261 1107 9 -0.04314984760136298 1107 10-11 0.12255157495620495 1107 12-13 0.048617856434077567 1107 14-15 -0.01368010742500303 1107 16-17 9.074557161028451E-4 1107 18-19 0.055629668627965145 1107 20-21 -0.0388216061182689 1107 22-23 -0.026910475494737796 1107 24-25 0.031072803863231968 1107 26-27 -0.07454596946004699 1107 28-29 -0.119388360451822 1107 30-31 -0.06803768435455737 1107 32-33 0.009998754173061286 1107 34-35 0.06770737870795784 1107 36-37 -0.040870205793247294 1107 38-39 -0.10075708767768532 1107 40-41 -0.20206500030509034 1107 42-43 -0.22484745730493927 1107 44-45 -0.31753991237859935 1107 46-47 -0.15168609424181767 1107 48-49 -0.2546902629179755 1107 50-51 -0.21132756184022128 1107 52-53 -0.3435201225644775 1107 54-55 -0.3656814950514473 1107 56-57 -0.35544814373777456 1107 58-59 -0.25066572803673637 1107 60-61 -0.3359904524967803 1107 62-63 -0.5084731951984338 1107 64-65 -0.5287419833054976 1107 66-67 -0.7304295359201234 1107 68-69 -0.5283818701288183 1107 70-71 -0.8042730120425361 1107 72-73 -0.8598601260329559 1107 74-75 -0.6843539907211849 1107 76-77 -0.6760985173583123 1107 78-79 -0.600012532877642 1107 80-81 -0.3901526506147981 1107 82-83 -0.4940548527537274 1107 84-85 -0.9171403872234762 1107 86-87 -1.0966629064224875 1107 88-89 -1.1153888959000646 1107 90-91 -1.1720096185277775 1107 92-93 -1.2409517505172403 1107 94-95 -1.4223437005666177 1107 96-97 -1.3289088986802433 1107 98-99 -1.6812993326572752 1107 100-101 -2.092966708626353 1108 1 -0.0585420785973767 1108 2 -0.020741846460559543 1108 3 0.018873983216877832 1108 4 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0.24527065155525563 2316 68-69 0.20845928793360002 2316 70-71 0.458985755062578 2316 72-73 0.7137783807045359 2316 74-75 0.8808141869351367 2316 76-77 0.7726432996617376 2316 78-79 0.769701845685514 2316 80-81 0.828985812758944 2316 82-83 0.7912220736509603 2316 84-85 1.1181107437477351 2316 86-87 1.45587716391249 2316 88-89 1.7094294305470576 2316 90-91 1.6927213565258938 2316 92-93 1.7190015674610954 2316 94-95 1.9434478867283858 2316 96-97 2.510166503857068 2316 98-99 3.089475246791462 2316 100-101 3.5615541082027597 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 4.0 4 15.0 5 22.0 6 70.0 7 163.0 8 390.0 9 751.0 10 1312.0 11 2142.0 12 3292.0 13 5092.0 14 6972.0 15 9492.0 16 12340.0 17 16799.0 18 17394.0 19 14736.0 20 14372.0 21 15034.0 22 16450.0 23 17944.0 24 20043.0 25 22798.0 26 25763.0 27 30763.0 28 36596.0 29 43019.0 30 51870.0 31 62759.0 32 77051.0 33 97620.0 34 129424.0 35 177293.0 36 241423.0 37 271300.0 38 124685.0 39 4692.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.907632883067734 18.67199325078782 15.143735696575577 23.276638169568866 2 15.92795748252033 19.11466879771557 40.19818245791295 24.75919126185115 3 19.972921523317012 22.36137550517647 29.28413822238963 28.381564749116894 4 13.350959706391109 16.063177569379988 35.60039621168698 34.98546651254193 5 14.205482380244701 36.65947354491969 33.95130137822927 15.18374269660634 6 34.149333112787126 35.09503103021589 16.19602317007612 14.559612686920866 7 30.009599926712287 32.010888823433234 19.08674096802124 18.892770281833236 8 26.83462615315223 35.509104662103574 18.346993136275064 19.309276048469133 9 25.936978930419297 16.97628391862774 19.687483896743213 37.399253254209746 10-11 25.679358630741266 26.217183550725977 26.205955008216243 21.897502810316517 12-13 26.33809940357853 23.150536779324053 27.078330019880713 23.433033797216698 14-15 22.920827006012516 24.87083995223575 25.288109132526703 26.920223909225026 16-17 22.64075620495353 28.089530228412524 26.26200099373413 23.007712572899813 18-19 22.588837492771425 26.36814860101267 28.692934034062244 22.35007987215366 20-21 22.875053995611644 25.538508811384027 29.317915346332146 22.268521846672186 22-23 22.765027002577156 25.5028510319126 29.061980918475694 22.67014104703455 24-25 22.661658933138995 25.653416022223702 29.07530718068144 22.609617863955865 26-27 22.197470345470023 26.049409581839633 28.881336066856793 22.871784005833554 28-29 22.39129681004128 26.361098148195865 28.785274637253096 22.46233040450976 30-31 22.453151889352256 25.98218006342905 28.690797698231684 22.873870348987012 32-33 22.186009554121895 25.925623152300385 28.763867886177568 23.12449940740015 34-35 22.835964475289025 26.061793502914902 28.637265062977534 22.464976958818543 36-37 22.94019282049194 25.63665147993497 28.543781906530192 22.8793737930429 38-39 22.3961683293603 26.111479043443797 28.52812646523485 22.964226161961058 40-41 23.036610576869553 25.86887017850571 28.48282076998857 22.61169847463617 42-43 22.643809349309535 25.860362200175846 28.409034518824463 23.08679393169016 44-45 22.65579523210002 26.18769669829956 28.523169922519877 22.633338147080547 46-47 22.99013604684821 26.414877678710592 27.788960388323574 22.806025886117624 48-49 22.817545204555685 26.428597557387402 27.77698078577756 22.976876452279352 50-51 22.96242619652996 26.699511682746156 27.671660962580795 22.66640115814309 52-53 23.205213239235448 26.447772968966465 27.566672551760995 22.780341240037092 54-55 22.90250616098306 26.61853247880676 27.674369260710662 22.80459209949952 56-57 22.840886748717413 26.812530183650214 27.564075236585733 22.78250783104664 58-59 22.842824517295774 26.432999832645145 27.51180233545931 23.21237331459977 60-61 22.624992723410386 26.659134004720002 27.670826490158074 23.045046781711537 62-63 22.66717317008143 26.69807496886849 27.776367288787757 22.85838457226232 64-65 22.43999454040277 26.71944701534064 27.70895128963895 23.13160715461764 66-67 22.410012801685852 26.667877694271308 27.635925653509517 23.28618385053332 68-69 22.816251306981364 26.37194064538628 27.75939324245292 23.05241480517943 70-71 22.700283944038368 26.448377064957167 27.362692775731933 23.488646215272528 72-73 22.734351987163866 26.565087577211795 27.374789494679074 23.325770940945265 74-75 22.662682229007178 26.913438378177347 27.17259862389918 23.251280768916295 76-77 22.760712168853285 26.554222525246324 27.428319086923196 23.25674621897719 78-79 22.793609501494792 26.703322646866795 27.37476916341385 23.128298688224564 80-81 22.90231247156732 26.71905172441218 27.116043507169014 23.26259229685149 82-83 22.888533347588925 26.8428378050713 27.135809351796876 23.1328194955429 84-85 22.82689134663537 26.890837580231864 27.10441802589691 23.177853047235864 86-87 22.964177517312084 26.79977102833922 26.990941364354175 23.245110089994515 88-89 22.922945057294555 26.928727964121542 26.736717385881764 23.411609592702135 90-91 22.890292774268065 26.817664774019885 26.839237447360926 23.452805004351127 92-93 22.904624622293294 26.562012306151388 26.960169342417277 23.573193729138037 94-95 22.722583079432454 26.48221431895974 27.04541456125387 23.749788040353938 96-97 22.98965031476634 26.162064897731163 27.020848259336418 23.827436528166086 98-99 22.717577097177806 26.369892756353547 26.690882460823506 24.22164768564514 100-101 23.331492461438717 25.6827079140737 26.73114596184178 24.254653662645804 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 266.0 1 206.5 2 217.5 3 276.5 4 240.0 5 236.5 6 488.0 7 1162.5 8 1855.5 9 2113.5 10 1792.0 11 1328.0 12 1252.5 13 1392.5 14 1520.0 15 1630.5 16 1761.0 17 1868.0 18 2046.0 19 2305.0 20 2552.0 21 2939.0 22 3467.5 23 4213.0 24 5311.5 25 6643.5 26 8437.5 27 10562.0 28 12289.0 29 13818.5 30 15860.0 31 18880.5 32 22994.5 33 25291.5 34 26024.0 35 28037.0 36 31191.5 37 34061.5 38 36110.5 39 38320.5 40 40867.5 41 43814.0 42 45848.0 43 47829.5 44 50543.0 45 53401.5 46 55219.5 47 57294.5 48 58737.0 49 58547.0 50 58966.5 51 58444.5 52 58139.5 53 59119.5 54 60206.5 55 57506.0 56 52471.0 57 48151.0 58 45039.5 59 40713.5 60 33189.0 61 27328.5 62 22863.0 63 17261.5 64 12422.0 65 9460.5 66 7115.0 67 5309.5 68 3965.0 69 3085.0 70 2432.5 71 1696.0 72 1190.5 73 921.0 74 651.5 75 444.0 76 344.0 77 220.0 78 122.5 79 74.5 80 45.5 81 23.0 82 15.0 83 8.5 84 3.5 85 2.5 86 1.5 87 0.5 88 0.5 89 1.0 90 1.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.009415435078984685 2 0.01297803213589781 3 0.010115230929449763 4 0.0 5 9.542670688160153E-4 6 0.0020357697468074997 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 7.634136550528122E-4 14-15 0.0 16-17 0.0024174765743339055 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.00327631693626832 26-27 0.01838554552585523 28-29 0.006075500338128632 30-31 0.003308125838562187 32-33 0.0 34-35 0.008652021423931873 36-37 6.679869481712108E-4 38-39 0.002544712183509374 40-41 0.002067578649101367 42-43 0.0031172724247989832 44-45 0.0 46-47 1.2723560917546873E-4 48-49 2.862801206448046E-4 50-51 0.0038488771775579285 52-53 0.007602327648234255 54-55 0.0063935893610673035 56-57 0.016413393583635465 58-59 0.02392029452498812 60-61 0.0041669662004966005 62-63 0.020866639904776867 64-65 0.02178909807129902 66-67 0.013932299204713824 68-69 0.0329540227764464 70-71 0.017463087359333083 72-73 0.0047077175394923424 74-75 0.017335851750157612 76-77 0.020453124174956597 78-79 0.027292038168138043 80-81 0.012214618480844996 82-83 0.03651661983335952 84-85 0.022138995996531557 86-87 0.03266774265580159 88-89 0.022838791846996637 90-91 0.028914292185125267 92-93 0.025224459519036672 94-95 0.015490935417113319 96-97 0.01558636212399492 98-99 0.011801102751024722 100-101 0.010687791170739373 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1571887.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.41510731146769 #Duplication Level Percentage of deduplicated Percentage of total 1 78.97932207689996 46.925648965859814 2 12.282688003270069 14.595544515751364 3 3.779785309437369 6.7372904932359114 4 1.6322949255689265 3.8793191266656786 5 0.8696222629768081 2.583435003760422 6 0.5218080993428226 1.8601970531068082 7 0.3628168295793314 1.5089760604703726 8 0.25715202857235564 1.2222972298390498 9 0.19125909127663102 1.0227311489245345 >10 1.030760086631319 11.146810295467574 >50 0.06148861643092833 2.4691179463754964 >100 0.026604547422443618 3.000361684372744 >500 0.00289632463314624 1.1922971500975679 >1k 0.00128725539250944 1.159282239970314 >5k 2.1454256541824E-4 0.6966910861023422 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5905 0.3756631360905714 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5026 0.3197430858579529 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3342 0.21261070293220824 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 1712 0.10891368145420123 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 1686 0.10725961853492014 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 6.361780458773437E-5 0.0 0.0 0.0 0.0 16-17 6.361780458773437E-5 0.0 0.0 3.180890229386718E-5 6.361780458773437E-5 18-19 9.542670688160156E-5 0.0 0.0 6.361780458773437E-5 6.361780458773437E-5 20-21 1.2723560917546873E-4 0.0 0.0 1.2723560917546873E-4 6.361780458773437E-5 22-23 1.2723560917546873E-4 0.0 0.0 1.2723560917546873E-4 6.361780458773437E-5 24-25 2.5447121835093747E-4 0.0 0.0 3.8170682752640617E-4 6.361780458773437E-5 26-27 2.5447121835093747E-4 0.0 0.0 8.270314596405468E-4 1.2723560917546873E-4 28-29 3.180890229386718E-4 0.0 0.0 0.0027037566949787103 1.2723560917546873E-4 30-31 3.8170682752640617E-4 0.0 0.0 0.009447243981278552 1.2723560917546873E-4 32-33 3.8170682752640617E-4 0.0 0.0 0.02449285476627773 1.2723560917546873E-4 34-35 3.8170682752640617E-4 0.0 0.0 0.04450065430912019 1.2723560917546873E-4 36-37 3.8170682752640617E-4 0.0 0.0 0.07885426878649673 1.2723560917546873E-4 38-39 4.7713353440800773E-4 0.0 0.0 0.1384959605874977 1.2723560917546873E-4 40-41 5.089424367018749E-4 0.0 0.0 0.20405410821515796 1.2723560917546873E-4 42-43 5.407513389957421E-4 0.0 0.0 0.26668583683178243 1.2723560917546873E-4 44-45 5.725602412896092E-4 0.0 0.0 0.3430908201416514 1.2723560917546873E-4 46-47 6.043691435834765E-4 0.0 0.0 0.43422332521358087 1.2723560917546873E-4 48-49 6.361780458773436E-4 0.0 0.0 0.5304770635548229 1.2723560917546873E-4 50-51 6.361780458773436E-4 0.0 0.0 0.6335060980846587 1.9085341376320309E-4 52-53 6.361780458773436E-4 0.0 0.0 0.7307141034947169 1.9085341376320309E-4 54-55 6.361780458773436E-4 0.0 0.0 0.83676498374247 1.9085341376320309E-4 56-57 6.997958504650779E-4 0.0 0.0 0.9602789513495563 1.9085341376320309E-4 58-59 6.997958504650779E-4 0.0 0.0 1.0873237071112618 1.9085341376320309E-4 60-61 6.997958504650779E-4 0.0 0.0 1.231449843404774 1.9085341376320309E-4 62-63 6.997958504650779E-4 0.0 0.0 1.3652062775504854 1.9085341376320309E-4 64-65 6.997958504650779E-4 0.0 0.0 1.5041793716723912 1.9085341376320309E-4 66-67 7.634136550528123E-4 0.0 0.0 1.6602974641306913 1.9085341376320309E-4 68-69 7.634136550528123E-4 0.0 0.0 1.8338468350460306 1.9085341376320309E-4 70-71 7.634136550528123E-4 0.0 0.0 2.012962763862797 1.9085341376320309E-4 72-73 7.634136550528123E-4 0.0 0.0 2.203307235189298 1.9085341376320309E-4 74-75 7.634136550528123E-4 0.0 0.0 2.382168692787713 1.9085341376320309E-4 76-77 7.634136550528123E-4 0.0 0.0 2.5788431356706942 1.9085341376320309E-4 78-79 8.270314596405468E-4 3.180890229386718E-5 0.0 2.8057042268305548 1.9085341376320309E-4 80-81 9.542670688160153E-4 6.361780458773437E-5 0.0 3.041503619534992 2.2266231605707026E-4 82-83 9.542670688160153E-4 6.361780458773437E-5 0.0 3.276666834193552 2.5447121835093747E-4 84-85 9.860759711098825E-4 6.361780458773437E-5 0.0 3.5119890933635816 2.5447121835093747E-4 86-87 0.0010178848734037499 6.361780458773437E-5 0.0 3.7571721122447097 2.5447121835093747E-4 88-89 0.0010178848734037499 6.361780458773437E-5 0.0 4.022999108714558 2.5447121835093747E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2660 0.0 56.07385 1 GTATCAA 4780 0.0 43.62636 1 ATCAACG 6180 0.0 33.738003 3 TATCAAC 6170 0.0 33.64087 2 TCAACGC 6235 0.0 33.363155 4 CAACGCA 6380 0.0 32.604904 5 TATACCG 60 0.0039542476 31.661991 5 AACGCAG 6625 0.0 31.327448 6 TGGTATC 1145 0.0 28.62296 2 ACGCAGA 7720 0.0 26.760094 7 CGCAGAG 7810 0.0 26.208483 8 GTGGTAT 1350 0.0 26.038162 1 GCAGAGT 8590 0.0 23.386374 9 GAGTACT 5615 0.0 21.526207 12-13 TAAGGTG 900 0.0 21.107994 5 CAGAGTA 8110 0.0 21.081633 10-11 AGAGTAC 7710 0.0 20.697004 10-11 GTGTAGC 1040 0.0 20.553772 1 GTACTTT 5925 0.0 20.239626 14-15 GTACATG 4735 0.0 20.064224 1 >>END_MODULE