FastQCFastQC Report
Thu 26 May 2016
SRR937943_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937943_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences93313
Sequences flagged as poor quality0
Sequence length101
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGT28573.061738450162357No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26762.8677676208031038No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22012.358728151490146No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19222.0597344421463246No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16131.7285908715827376No Hit
ATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTT14841.590346468337745RNA PCR Primer, Index 31 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCT8370.8969811280314641RNA PCR Primer, Index 31 (95% over 24bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6420.6880070301029867No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6050.648355534598609No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3860.4136615476943191No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3740.4008016032064128No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3440.3686517419866471No Hit
CATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCT3330.35686345953939963RNA PCR Primer, Index 31 (96% over 27bp)
TTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTC3260.34936182525478765No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2890.3097103297504099No Hit
CACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTG2760.29577872322184473RNA PCR Primer, Index 38 (95% over 24bp)
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2000.2143324081317716No Hit
AATCTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGCAAGTTTAAAATT1820.19504249139991212No Hit
TATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCT1730.1853975330339824RNA PCR Primer, Index 31 (95% over 21bp)
ATTTTAAACTTGCTAAAAAAACAACAAAATCAAAAAGTAAGTTTAGATTA1610.1725375885460761No Hit
CTTTACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTC1490.1596776440581698No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA1420.1521760097735578No Hit
TTCCAGAAGAGCTGTCCCTCTTTTGGCTATAATCTAAACTTACTTTTTGA1220.13074276896038065No Hit
GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAG1190.12752778283840407No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT1140.1221694726351098No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG1140.1221694726351098No Hit
ACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGC1070.11466783835049779RNA PCR Primer, Index 38 (96% over 25bp)
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1040.11145285222852121No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG1010.10823786610654465No Hit
GTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1010.10823786610654465No Hit
GTACATGGGGTGAGAAGATTTTTAGGTAGAGGTGAAAAGCCTAACGAGCT1000.1071662040658858No Hit
CTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTC950.1018078938625915No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTACA554.928177E-543.181821
GGTATCA12850.042.879381
TTGGTAT308.264063E-539.58333616-17
CTATTAA801.0670428E-535.6251
ATGGGAT550.00256754534.5454565
GGGTGAG550.00256754534.5454568
GTAGACC550.00256754534.5454563
ATGGGAG600.003932533331.6666685
CCTCGGG300.00394254931.66666850-51
AGACCAG600.003932533331.6666685
AACCTCG300.00394254931.66666848-49
TGTAGAC600.003932533331.6666682
GTATCAA18800.030.5718081
GGTGAGA650.005816951429.2307669
TAAAGGT1153.8147573E-628.9130425
ATGGGTA856.362816E-427.9411755
CATGGGT1205.3124986E-627.7083344
ATGGGGA1554.4930857E-827.5806455
TGGGGAA1257.296152E-626.6000026
ATGGGGT1658.238203E-825.9090925
Produced by FastQC (version 0.11.5)