##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937943_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 93313 Sequences flagged as poor quality 0 Sequence length 101 %GC 37 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.24550705689454 33.0 31.0 34.0 31.0 34.0 2 32.44188912584527 34.0 31.0 34.0 31.0 34.0 3 32.429404263071596 34.0 31.0 34.0 30.0 34.0 4 35.98737582116104 37.0 35.0 37.0 35.0 37.0 5 35.95344700095378 37.0 35.0 37.0 35.0 37.0 6 36.07304448469131 37.0 35.0 37.0 35.0 37.0 7 36.009055544243566 37.0 35.0 37.0 35.0 37.0 8 36.05002518405796 37.0 35.0 37.0 35.0 37.0 9 37.62796180596487 39.0 37.0 39.0 35.0 39.0 10-11 37.64714455649266 39.0 37.0 39.0 35.0 39.0 12-13 37.55589789204076 39.0 37.0 39.0 35.0 39.0 14-15 38.574716277474735 40.0 38.0 41.0 34.0 41.0 16-17 38.49437913259675 40.0 38.0 41.0 34.0 41.0 18-19 38.78281161252987 40.0 38.0 41.0 35.0 41.0 20-21 38.856938475882245 40.0 38.0 41.0 35.0 41.0 22-23 38.68436873747495 40.0 38.0 41.0 35.0 41.0 24-25 38.484600216475734 40.0 38.0 41.0 34.5 41.0 26-27 38.1925883853268 40.0 37.0 41.0 34.0 41.0 28-29 37.90999646351527 40.0 36.0 41.0 33.5 41.0 30-31 37.30712226592222 39.0 35.0 41.0 33.0 41.0 32-33 36.63570456420863 38.0 35.0 41.0 33.0 41.0 34-35 36.07841351151501 38.0 35.0 41.0 31.0 41.0 36-37 35.67109084479119 38.0 35.0 41.0 31.0 41.0 38-39 35.15831127495633 38.0 35.0 40.0 29.5 41.0 40-41 34.70922058019783 38.0 35.0 40.0 26.5 41.0 42-43 34.043316579683434 37.0 35.0 40.0 22.0 41.0 44-45 33.22293785431826 37.0 33.5 40.0 8.0 41.0 46-47 32.125555924683596 36.5 33.0 40.0 2.0 41.0 48-49 31.133223666584506 36.0 32.5 40.0 2.0 41.0 50-51 30.23576564894495 35.0 30.5 40.0 2.0 41.0 52-53 29.508959094659907 35.0 28.5 40.0 2.0 41.0 54-55 28.930031185365383 35.0 26.5 39.0 2.0 41.0 56-57 28.214857522531695 35.0 22.5 39.0 2.0 41.0 58-59 27.40648677033211 35.0 13.5 39.0 2.0 40.5 60-61 26.583193124216347 34.5 2.0 38.0 2.0 40.0 62-63 25.73290966960659 34.0 2.0 38.0 2.0 40.0 64-65 24.95172698337852 33.5 2.0 37.5 2.0 40.0 66-67 24.082555485302155 33.0 2.0 37.0 2.0 39.5 68-69 23.236773011263168 33.0 2.0 36.0 2.0 39.0 70-71 22.615980624350307 32.0 2.0 35.5 2.0 39.0 72-73 22.11071876373067 31.0 2.0 35.0 2.0 38.0 74-75 21.66361600205759 31.0 2.0 35.0 2.0 37.0 76-77 20.61047228146132 28.5 2.0 34.0 2.0 35.5 78-79 21.09871614887529 30.0 2.0 35.0 2.0 36.0 80-81 20.87489953168369 30.0 2.0 35.0 2.0 36.0 82-83 20.514044131042837 29.5 2.0 35.0 2.0 35.0 84-85 20.1374406567145 29.0 2.0 34.5 2.0 35.0 86-87 19.679723082528696 28.5 2.0 34.0 2.0 35.0 88-89 19.203390738696644 27.0 2.0 34.0 2.0 35.0 90-91 18.860174895245038 26.5 2.0 34.0 2.0 35.0 92-93 18.276311982253276 24.5 2.0 34.0 2.0 35.0 94-95 17.72612069057902 22.5 2.0 34.0 2.0 35.0 96-97 17.120813820153675 17.0 2.0 33.0 2.0 35.0 98-99 16.182589778487454 2.0 2.0 33.0 2.0 35.0 100-101 13.501795033918103 2.0 2.0 29.0 2.0 33.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.015052986088221587 1101 2 0.011208823524363254 1101 3 0.01933622549968561 1101 4 0.007603581233269097 1101 5 0.10128301377315552 1101 6 0.14230853572907165 1101 7 0.14589056396953026 1101 8 0.2883146579106324 1101 9 -0.13440454161083437 1101 10-11 0.13183028802320962 1101 12-13 0.015526856503129238 1101 14-15 0.08892666940241867 1101 16-17 -0.12403023877120489 1101 18-19 -0.43347242062757374 1101 20-21 -0.5958221468368095 1101 22-23 -0.11517459963499022 1101 24-25 -0.24854176110129345 1101 26-27 -0.18419755378650393 1101 28-29 0.0942563668581613 1101 30-31 0.1877231287602683 1101 32-33 -0.09051372491536114 1101 34-35 -0.06171141691434201 1101 36-37 0.36318301097226424 1101 38-39 0.9283129258699034 1101 40-41 0.7122068040076854 1101 42-43 0.4157253398694678 1101 44-45 0.6072318087772999 1101 46-47 1.677060772880452 1101 48-49 2.20178734035494 1101 50-51 3.2744700075779 1101 52-53 2.621852106721473 1101 54-55 2.664958926401166 1101 56-57 3.433673047629714 1101 58-59 3.2076821799343236 1101 60-61 2.860448503196615 1101 62-63 1.9313536761503727 1101 64-65 2.724562644929623 1101 66-67 2.2802783034972975 1101 68-69 3.0017893271541993 1101 70-71 3.095757350059408 1101 72-73 1.9683118631595313 1101 74-75 2.430273223704809 1101 76-77 2.798081891903255 1101 78-79 2.2085665013652864 1101 80-81 2.9856499854511576 1101 82-83 2.6315360041406706 1101 84-85 1.9433186051761453 1101 86-87 1.532869793404128 1101 88-89 2.211789755496252 1101 90-91 2.6242051033276717 1101 92-93 2.80318953872251 1101 94-95 2.5648123156865665 1101 96-97 2.309860989907918 1101 98-99 2.7084218759375993 1101 100-101 2.3299096817384335 1102 1 0.26304089726881585 1102 2 0.3092173583750011 1102 3 -0.22426263652022982 1102 4 -0.07312188107113826 1102 5 0.07674531818282304 1102 6 0.3108718358713176 1102 7 0.11032868630238823 1102 8 -0.06623726243076078 1102 9 0.001441831077642064 1102 10-11 0.3971218954200779 1102 12-13 0.38288105280469864 1102 14-15 0.02633776470825211 1102 16-17 0.10267673135681576 1102 18-19 0.27349770739517965 1102 20-21 5.505416411395458E-4 1102 22-23 0.4086518584019885 1102 24-25 0.22478683064834826 1102 26-27 0.3981281930129299 1102 28-29 0.21143275377139759 1102 30-31 1.0207601131130417 1102 32-33 1.1626868440746847 1102 34-35 0.8470723668694475 1102 36-37 0.6112271077005715 1102 38-39 0.7713072359694806 1102 40-41 0.5310190372936106 1102 42-43 0.5937125375935111 1102 44-45 1.3776798884358996 1102 46-47 0.1887961925106083 1102 48-49 0.863416074352088 1102 50-51 -0.13129101660417675 1102 52-53 0.09767002990781393 1102 54-55 0.031246266372715326 1102 56-57 0.20661045872502015 1102 58-59 0.10224121265125063 1102 60-61 0.07364195981112331 1102 62-63 0.17086292223856603 1102 64-65 1.0122582352567946 1102 66-67 0.9175471512924602 1102 68-69 1.331625742516927 1102 70-71 1.073353367129105 1102 72-73 0.8274868275976495 1102 74-75 1.3988009619693926 1102 76-77 0.537591137991452 1102 78-79 0.8099178527166373 1102 80-81 1.1689714648252725 1102 82-83 1.2634705702857616 1102 84-85 1.0587168982060184 1102 86-87 0.7869594093358465 1102 88-89 0.9173374084123296 1102 90-91 1.0484583038966626 1102 92-93 0.5690145742843917 1102 94-95 1.1602959572228393 1102 96-97 1.7401597096803272 1102 98-99 2.0770207379575147 1102 100-101 2.025677818296044 1103 1 0.3329733297012538 1103 2 0.49739303405068114 1103 3 0.18249412023652667 1103 4 0.15694568649642804 1103 5 0.4341777506152553 1103 6 0.2429664304659127 1103 7 0.36168003765374124 1103 8 0.08173571054221185 1103 9 0.4392796689154821 1103 10-11 0.3160408143389972 1103 12-13 0.5421715933452376 1103 14-15 0.8922161430866282 1103 16-17 0.9753118664919498 1103 18-19 0.4461328425303108 1103 20-21 0.444638379478981 1103 22-23 0.8282464529965878 1103 24-25 0.6070503441618627 1103 26-27 0.4447498146345481 1103 28-29 0.5712300510686887 1103 30-31 0.015354707707636805 1103 32-33 -0.0401847775469335 1103 34-35 0.6070385830856608 1103 36-37 0.5168014320248915 1103 38-39 0.30627345218569957 1103 40-41 1.0349041724287389 1103 42-43 0.8239490240799938 1103 44-45 0.16315286140887508 1103 46-47 -0.007149753435342632 1103 48-49 0.09652418246020034 1103 50-51 0.12512790231474114 1103 52-53 -0.41466105117326535 1103 54-55 -0.1564226525462047 1103 56-57 -0.4150111628966009 1103 58-59 -0.07225203059199004 1103 60-61 0.841040608459771 1103 62-63 0.6570115708872137 1103 64-65 1.2485758028243659 1103 66-67 1.082580935076244 1103 68-69 1.1228419587331437 1103 70-71 0.6747047184804593 1103 72-73 1.4038381789490053 1103 74-75 1.6384969079153358 1103 76-77 1.6049897866400968 1103 78-79 2.3460665013652857 1103 80-81 2.4902552486090563 1103 82-83 2.6687070567722486 1103 84-85 2.7485817630708844 1103 86-87 3.1565540039304416 1103 88-89 2.187776597601516 1103 90-91 2.495586682275043 1103 92-93 2.367663222933036 1103 94-95 2.4881675788444575 1103 96-97 2.6325583583289713 1103 98-99 3.6077639812007583 1103 100-101 4.811488629106856 1104 1 0.061326988237837554 1104 2 0.3507466925872649 1104 3 0.6056648519438426 1104 4 0.07981154015496372 1104 5 -0.2591149323115687 1104 6 0.003027406075666761 1104 7 -0.12368581600479445 1104 8 -0.27619111872607505 1104 9 0.19293820550084462 1104 10-11 0.06604081433899722 1104 12-13 0.11244598358914004 1104 14-15 0.6303258991841929 1104 16-17 0.13415332990658158 1104 18-19 0.30497430594494546 1104 20-21 0.1920469160643492 1104 22-23 0.10080742860633762 1104 24-25 0.4151296124545496 1104 26-27 0.5136522536589467 1104 28-29 0.35629102667844137 1104 30-31 -0.2322062679021144 1104 32-33 -0.49552014340059003 1104 34-35 -0.6745162949631194 1104 36-37 -0.9485949094385191 1104 38-39 -0.8661350843996729 1104 40-41 -0.9253092422054081 1104 42-43 -0.9624839027492698 1104 44-45 -1.7286154312740507 1104 46-47 -3.275442436362173 1104 48-49 -2.7723782565641883 1104 50-51 -2.1087135610998935 1104 52-53 -1.2212159292220477 1104 54-55 -1.0096238720583983 1104 56-57 -1.4942794555795267 1104 58-59 -1.5922215427871151 1104 60-61 -1.308197196418277 1104 62-63 -1.2353664778932725 1104 64-65 -1.4994424898585628 1104 66-67 -0.9815958941920471 1104 68-69 0.08442732458680524 1104 70-71 -0.3252952815195407 1104 72-73 -0.4004301137339219 1104 74-75 0.5825517859641174 1104 76-77 0.015508079323023338 1104 78-79 0.03783479404821222 1104 80-81 0.45885280958466623 1104 82-83 0.4389814470161504 1104 84-85 0.7772402996562491 1104 86-87 1.1681393697841003 1104 88-89 0.6796973293088335 1104 90-91 1.0670805847140663 1104 92-93 1.4920534668354755 1104 94-95 2.409966359332266 1104 96-97 2.711979090036291 1104 98-99 2.4117273958349053 1104 100-101 1.9950252144727099 1105 1 0.06909970332762327 1105 2 0.3420358911935324 1105 3 0.5324941202365281 1105 4 0.22521491726566012 1105 5 0.15203489347240406 1105 6 0.05931258431206743 1105 7 0.04959212556583026 1105 8 0.2254170292235358 1105 9 0.05356538320119597 1105 10-11 0.20065619895438402 1105 12-13 0.3739710438946844 1105 14-15 0.31130954968003977 1105 16-17 0.520778899458989 1105 18-19 0.1806108645083384 1105 20-21 0.26970706079766416 1105 22-23 0.2092904090405412 1105 24-25 0.3399486958102145 1105 26-27 0.37963992452466044 1105 28-29 0.10763114996978373 1105 30-31 -0.049480457127529576 1105 32-33 0.6042520356398811 1105 34-35 0.8242775940746654 1105 36-37 1.152584399057858 1105 38-39 0.42433663899888074 1105 40-41 0.4243959306704994 1105 42-43 0.9236056174865936 1105 44-45 1.2544990152550284 1105 46-47 1.73186123557565 1105 48-49 0.6665791275151456 1105 50-51 1.0211443858312244 1105 52-53 0.6457098279476092 1105 54-55 0.016173501299949322 1105 56-57 0.9641097162242787 1105 58-59 1.5348221452321837 1105 60-61 0.8266862128553782 1105 62-63 1.2178632192388648 1105 64-65 1.3463093193078812 1105 66-67 -0.029438295692987282 1105 68-69 -0.1774327665415818 1105 70-71 0.19668274045848122 1105 72-73 0.0030140031248286903 1105 74-75 -0.10305528988686063 1105 76-77 0.26316286356317775 1105 78-79 -0.044180751381968975 1105 80-81 0.07363436948817537 1105 82-83 0.47523178204697203 1105 84-85 1.1296257191148378 1105 86-87 1.1480375204139612 1105 88-89 1.1394936305685484 1105 90-91 1.1685949240332825 1105 92-93 1.2094214646912782 1105 94-95 1.3725769195038033 1105 96-97 1.6772698967905129 1105 98-99 0.9493848603216364 1105 100-101 -0.5151597225414957 1106 1 0.18384542272450943 1106 2 -0.16626975664699728 1106 3 -0.3395989030192865 1106 4 -0.36601942978264645 1106 5 0.06731728549898008 1106 6 0.010117593256609325 1106 7 -0.0034362414160256094 1106 8 0.24394501286779757 1106 9 0.04858199449687817 1106 10-11 0.08348267480410954 1106 12-13 0.10365415148477553 1106 14-15 0.03262311983081645 1106 16-17 -0.7854439474615305 1106 18-19 -0.11462297142317368 1106 20-21 -0.4057976670326511 1106 22-23 -0.3979163377010906 1106 24-25 -0.16867640002418227 1106 26-27 -0.2418780923421906 1106 28-29 0.11919516734776181 1106 30-31 0.4583779635215919 1106 32-33 0.12827452477863943 1106 34-35 0.3074746295972872 1106 36-37 0.18555143202489432 1106 38-39 0.2084536847438372 1106 40-41 -0.34547373454800123 1106 42-43 -0.15235911545489245 1106 44-45 -0.033068068823681074 1106 46-47 0.09168745586698179 1106 48-49 0.23315208943694543 1106 50-51 0.08413953022171938 1106 52-53 0.6444959255709186 1106 54-55 0.9960482776863522 1106 56-57 0.5675469766382832 1106 58-59 -0.18169970501059396 1106 60-61 -0.4405000892146447 1106 62-63 -0.9479302895779043 1106 64-65 -1.4434300111291272 1106 66-67 -0.5312272044586415 1106 68-69 -1.1614603668482495 1106 70-71 -1.9880859791939578 1106 72-73 -1.5246501931440193 1106 74-75 -1.806415882782339 1106 76-77 -1.9184113761506012 1106 78-79 -1.7914334986347136 1106 80-81 -1.814686611856061 1106 82-83 -1.7907406176463532 1106 84-85 -2.0182787020453965 1106 86-87 -1.7521669263021131 1106 88-89 -1.2597524721659283 1106 90-91 -1.4394423874924023 1106 92-93 -1.3114065445088237 1106 94-95 -1.5320359095276324 1106 96-97 -0.9309590835314907 1106 98-99 -0.1099685769387797 1106 100-101 -0.2100229988001221 1107 1 0.21271470901159262 1107 2 0.07109993060240072 1107 3 0.10720676391468942 1107 4 0.22433074396769115 1107 5 0.020672003488819257 1107 6 -0.048556558039834385 1107 7 0.34099038248132985 1107 8 -0.0013102664692823396 1107 9 -0.005547917291416127 1107 10-11 0.14362702123555238 1107 12-13 0.07284688070156164 1107 14-15 0.11735982124754685 1107 16-17 0.38436359062988856 1107 18-19 0.2678282448291682 1107 20-21 -0.020807022819873566 1107 22-23 -0.20652366194594407 1107 24-25 0.30439229818485813 1107 26-27 0.2410141824506411 1107 28-29 0.1236725798043139 1107 30-31 -0.1145303497636263 1107 32-33 -0.3238773062825686 1107 34-35 -0.29590681921319373 1107 36-37 -0.2914600622279764 1107 38-39 -0.9291432144809733 1107 40-41 -0.10396077009999516 1107 42-43 0.10031396660873071 1107 44-45 0.3765149303743911 1107 46-47 0.11066633851868346 1107 48-49 -0.11755627730991591 1107 50-51 -0.1405330172254864 1107 52-53 -0.6701926603686665 1107 54-55 -1.0284772502473523 1107 56-57 -1.5816778295632687 1107 58-59 -1.686979042086243 1107 60-61 -1.8006978972873533 1107 62-63 -1.3270401532507137 1107 64-65 -0.7391397144170178 1107 66-67 -1.632002398257093 1107 68-69 -1.4923879263243265 1107 70-71 -1.3482837872666664 1107 72-73 -1.3786330854188122 1107 74-75 -2.2098507932340894 1107 76-77 -2.2218061903713995 1107 78-79 -2.4695944181749425 1107 80-81 -2.2886240617357707 1107 82-83 -1.3822268512737281 1107 84-85 -1.608027432331415 1107 86-87 -1.2017793294028927 1107 88-89 -0.9509446667663006 1107 90-91 -1.1823587200238102 1107 92-93 -0.9116471218945499 1107 94-95 -0.7496916165578362 1107 96-97 -0.32670888305033685 1107 98-99 -0.37226475443142704 1107 100-101 -0.048568842157513004 1108 1 0.3471587229596764 1108 2 0.0017469666349470003 1108 3 0.4088986146185505 1108 4 0.2641086078447401 1108 5 0.08782943600851922 1108 6 0.2537810372074887 1108 7 -0.060791872458615615 1108 8 0.030331216160192298 1108 9 0.11456056779188373 1108 10-11 0.18682733119293005 1108 12-13 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2316 42-43 -0.43971248034478805 2316 44-45 -0.5497896164672298 2316 46-47 -1.2129019658247238 2316 48-49 -0.1280333396636948 2316 50-51 -0.5021951065348169 2316 52-53 -0.29978273258919685 2316 54-55 0.4065751350644149 2316 56-57 0.10268795214764737 2316 58-59 -0.19409937572473268 2316 60-61 -0.034346559681821986 2316 62-63 -0.5322583406172114 2316 64-65 -0.3161918962906789 2316 66-67 0.4358442094125259 2316 68-69 0.3310277994411095 2316 70-71 1.258775514940634 2316 72-73 1.1390151700994444 2316 74-75 1.5372247840215323 2316 76-77 1.1198681052241675 2316 78-79 1.1377700411882934 2316 80-81 0.6164720627683486 2316 82-83 0.9269482072147248 2316 84-85 0.925794152451413 2316 86-87 0.7067752428684955 2316 88-89 1.46050889848647 2316 90-91 2.0595800451069017 2316 92-93 2.0915570282427716 2316 94-95 2.4345724461010967 2316 96-97 2.537038446824546 2316 98-99 2.844600264386596 2316 100-101 2.8276390715847324 >>END_MODULE >>Per sequence quality scores pass #Quality Count 5 6.0 6 25.0 7 50.0 8 94.0 9 186.0 10 329.0 11 577.0 12 925.0 13 1420.0 14 1673.0 15 2213.0 16 2868.0 17 3723.0 18 3574.0 19 2602.0 20 2380.0 21 2366.0 22 2300.0 23 2323.0 24 2546.0 25 2553.0 26 1831.0 27 1249.0 28 1416.0 29 1793.0 30 2083.0 31 2461.0 32 2858.0 33 3654.0 34 5306.0 35 8597.0 36 8894.0 37 12005.0 38 6148.0 39 285.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.548991030188716 23.101818610483 14.5060173823583 17.84317297696998 2 15.618032927456696 23.76200480192077 40.80882352941176 19.811138741210772 3 18.726824563283678 32.0694459329118 33.9245525667131 15.279176937091416 4 10.311753169508417 23.966091886272785 33.735572440548275 31.98658250367052 5 8.285318827051212 42.79031340865441 34.11551378204096 14.808853982253408 6 22.07838136165379 40.82282211481787 16.49395046778048 20.604846055747856 7 18.29862934425 42.3199339856183 19.00806961516616 20.373367054965545 8 25.04045524203487 34.31354687985596 20.0775883317437 20.56840954636546 9 24.55392067557575 16.865817195889104 20.773096996131297 37.807165132403846 10-11 22.409524932217376 30.057976916399642 25.30301244199629 22.229485709386687 12-13 25.696848241938426 29.67003525768114 26.456120797745225 18.176995702635217 14-15 22.492042909348108 29.171176577754437 27.849281450601737 20.487499062295715 16-17 16.646662308574367 34.2813970186362 28.39690075337841 20.675039919411013 18-19 16.105472978041647 29.49964099321638 35.75600398658279 18.638882042159185 20-21 16.98262835832092 28.212039051364762 36.31005326160342 18.495279328710897 22-23 16.495110515740123 30.829202947086404 35.70314802411252 16.97253851306095 24-25 16.631659040005147 31.70726479697363 36.32880734731495 15.332268815706279 26-27 16.391685734035654 31.274414716615134 37.125908937460814 15.207990611888391 28-29 15.945259502963147 30.698294985693312 37.55210956672704 15.804335944616504 30-31 14.591214514590678 30.021004575996912 38.94527021958355 16.442510689828854 32-33 13.027659597269405 32.14396707854211 38.24011659683003 16.58825672735846 34-35 14.529154229922137 30.911896940694817 38.072246544951795 16.48670228443125 36-37 13.986261292638755 32.02929924019161 38.15974194378061 15.824697523389025 38-39 14.059427162875437 30.397342121480055 38.19950164777751 17.343729067867 40-41 14.076876497098242 30.56089941107437 38.570073575512694 16.792150516314685 42-43 15.766347478016707 29.657216039095697 38.69541686537812 15.88101961750947 44-45 14.214294134667938 30.762182379355057 38.19860466611654 16.824918819860464 46-47 16.047603227846068 29.20975641121816 36.08875505020737 18.65388531072841 48-49 15.56321198546826 28.85396461371942 36.073751781638144 19.509071619174176 50-51 14.260070944027092 28.89790275738643 36.910719835392705 19.931306463193767 52-53 15.057387502277281 29.230653821011003 37.561754525092965 18.150204151618745 54-55 15.170983678587122 29.009891440635283 35.117293410350115 20.701831470427486 56-57 16.44733316901182 30.450741054301115 32.76338773804293 20.33853803864413 58-59 14.867167490060334 31.603313579029717 34.492514440646 19.03700449026395 60-61 16.161735235176238 33.46907719181679 31.035332697480523 19.333854875526455 62-63 14.598180317854961 34.62915135083 30.646855207741687 20.125813123573348 64-65 14.276681705657305 34.16244253212307 30.660786814270253 20.900088947949374 66-67 14.921822254133938 33.635184808118915 30.060656071501292 21.38233686624586 68-69 14.973502730102936 32.0898281562294 30.54071577458298 22.395953339084677 70-71 15.150086268794272 30.987107905650873 30.911019900764096 22.95178592479076 72-73 15.475335698134238 29.567691532798218 31.10070408196071 23.856268687106834 74-75 15.253501655717852 28.964881634927607 30.90673325260146 24.87488345675308 76-77 14.845734249247158 28.302594493800438 32.40866760258485 24.44300365436756 78-79 15.080964067171776 27.975201740379152 31.859976637767513 25.083857554681554 80-81 15.190273595318981 27.502598780448594 31.91945388102408 25.387673743208346 82-83 15.02708669349437 27.589311070982674 31.50844215343232 25.87516008209063 84-85 14.929268031293539 27.057121423212948 30.983817382917156 27.02979316257636 86-87 15.145669565590152 27.075195181678374 30.739305865899336 27.03982938683214 88-89 15.48980313568313 27.001596776440586 29.91812502009366 27.59047506778262 90-91 15.506995536360177 25.91215256753064 30.752495726587327 27.82835616952186 92-93 15.329589660604631 25.709172355406 30.37411721839401 28.587120765595365 94-95 15.264218276124442 25.469655889318744 30.347325667377532 28.918800167179278 96-97 15.511236376496306 25.423574421570414 29.729512500937705 29.33567670099557 98-99 15.623760890765489 25.204419534255678 29.53232668545647 29.639492889522362 100-101 15.529990462207838 24.86631016042781 30.01618209681395 29.587517280550408 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1573.0 1 919.5 2 321.0 3 346.5 4 250.0 5 222.5 6 390.5 7 635.5 8 820.5 9 839.0 10 624.0 11 422.0 12 397.0 13 408.0 14 427.0 15 428.5 16 436.0 17 501.0 18 573.5 19 645.5 20 682.0 21 717.0 22 785.5 23 862.0 24 1016.0 25 1192.0 26 1450.5 27 1778.0 28 1890.0 29 2007.5 30 2217.5 31 2314.0 32 2471.0 33 2576.0 34 2636.0 35 2824.5 36 2979.0 37 2966.5 38 2682.5 39 2350.5 40 2205.5 41 2171.0 42 2198.0 43 2265.0 44 2407.5 45 2457.5 46 2425.5 47 2368.5 48 2440.5 49 2533.5 50 2703.5 51 2889.5 52 2555.0 53 1816.5 54 1217.0 55 958.0 56 808.0 57 703.0 58 676.5 59 674.0 60 627.5 61 603.0 62 601.0 63 587.0 64 500.5 65 414.0 66 400.5 67 382.5 68 355.0 69 324.5 70 265.0 71 212.5 72 188.0 73 163.0 74 115.5 75 89.5 76 65.5 77 44.5 78 36.5 79 24.5 80 18.0 81 11.0 82 6.0 83 4.5 84 1.5 85 1.5 86 1.5 87 1.0 88 0.5 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.018218254691200583 3 0.0032149861219765738 4 0.0021433240813177154 5 0.12002614855379208 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 5.358310203294289E-4 24-25 0.0 26-27 0.0037508171423060025 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.008037465304941434 36-37 0.0 38-39 0.005894141223623718 40-41 0.008037465304941434 42-43 0.0037508171423060025 44-45 0.0021433240813177154 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0016074930609882869 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0016074930609882869 84-85 0.0032149861219765738 86-87 0.0026791551016471446 88-89 0.0 90-91 0.0037508171423060025 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 93313.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.89658461307642 #Duplication Level Percentage of deduplicated Percentage of total 1 84.06786941580756 41.94699559546901 2 8.030498281786942 8.013888740046939 3 2.6975945017182132 4.038022569202576 4 1.4518900343642611 2.8977741579415515 5 0.7946735395189003 1.9825747752188871 6 0.5455326460481099 1.6332129499640993 7 0.38874570446735396 1.3577958055147727 8 0.30927835051546393 1.2345546708390043 9 0.21692439862542956 0.9741407949589017 >10 1.3380584192439862 12.254455434934039 >50 0.09450171821305842 3.247135983196339 >100 0.045103092783505154 4.519198825458403 >500 0.0064432989690721655 2.2333436927330594 >1k 0.012886597938144331 13.666906004522414 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGT 2857 3.061738450162357 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2676 2.8677676208031038 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2201 2.358728151490146 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1922 2.0597344421463246 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1613 1.7285908715827376 No Hit ATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTT 1484 1.590346468337745 RNA PCR Primer, Index 31 (95% over 22bp) ACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCT 837 0.8969811280314641 RNA PCR Primer, Index 31 (95% over 24bp) ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 642 0.6880070301029867 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 605 0.648355534598609 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 386 0.4136615476943191 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 374 0.4008016032064128 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 344 0.3686517419866471 No Hit CATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCT 333 0.35686345953939963 RNA PCR Primer, Index 31 (96% over 27bp) TTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTC 326 0.34936182525478765 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 289 0.3097103297504099 No Hit CACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTG 276 0.29577872322184473 RNA PCR Primer, Index 38 (95% over 24bp) GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 200 0.2143324081317716 No Hit AATCTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGCAAGTTTAAAATT 182 0.19504249139991212 No Hit TATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCT 173 0.1853975330339824 RNA PCR Primer, Index 31 (95% over 21bp) ATTTTAAACTTGCTAAAAAAACAACAAAATCAAAAAGTAAGTTTAGATTA 161 0.1725375885460761 No Hit CTTTACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTC 149 0.1596776440581698 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 142 0.1521760097735578 No Hit TTCCAGAAGAGCTGTCCCTCTTTTGGCTATAATCTAAACTTACTTTTTGA 122 0.13074276896038065 No Hit GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAG 119 0.12752778283840407 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 114 0.1221694726351098 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 114 0.1221694726351098 No Hit ACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGC 107 0.11466783835049779 RNA PCR Primer, Index 38 (96% over 25bp) AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 104 0.11145285222852121 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 101 0.10823786610654465 No Hit GTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 101 0.10823786610654465 No Hit GTACATGGGGTGAGAAGATTTTTAGGTAGAGGTGAAAAGCCTAACGAGCT 100 0.1071662040658858 No Hit CTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTC 95 0.1018078938625915 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.001071662040658858 0.0 12-13 0.0 0.0 0.0 0.001071662040658858 0.0 14-15 0.0 0.0 0.0 0.001071662040658858 0.0 16-17 0.0 0.0 0.0 0.001071662040658858 0.0 18-19 0.0 0.0 0.0 0.0026791551016471446 0.0 20-21 0.0 0.0 0.0 0.005358310203294289 0.0 22-23 0.0 0.0 0.0 0.01500326856922401 0.0 24-25 0.0 0.0 0.0 0.03590067836207174 0.0 26-27 0.0 0.0 0.0 0.07501634284612005 0.0 28-29 0.0 0.0 0.0 0.19397082935925328 0.0 30-31 0.0 0.0 0.0 0.4629580015646266 0.0 32-33 0.0 0.0 0.0 0.8926944798688285 0.0 34-35 0.0 0.0 0.0 1.4376346275438578 0.0 36-37 0.0 0.0 0.0 2.130464136829809 0.0 38-39 0.0 0.0 0.0 2.965824697523389 0.0 40-41 0.0 0.0 0.0 3.8697716288191355 0.0 42-43 0.0 0.0 0.0 4.574925251572664 0.0 44-45 0.0 0.0 0.0 5.2607889575943325 0.0 46-47 0.0 0.0 0.0 5.924683591782495 0.0 48-49 0.0 0.0 0.0 6.5542850406695745 0.0 50-51 0.0 0.0 0.0 7.203712237308842 0.0 52-53 0.0 0.0 0.0 7.745973229882225 0.0 54-55 0.0 0.0 0.0 8.25662019225617 0.0 56-57 0.0 0.0 0.0 8.8015603399312 0.0 58-59 0.0 0.0 0.0 9.378114517805665 0.0 60-61 0.0 0.0 0.0 9.97395861241199 0.0 62-63 0.0 0.0 0.0 10.485677236826593 0.0 64-65 0.0 0.0 0.0 10.970068479204398 0.0 66-67 0.0 0.0 0.0 11.538585191773922 0.0 68-69 0.0 0.0 0.0 12.163364161478036 0.0 70-71 0.0 0.0 0.0 12.795108934446432 0.0 72-73 0.0 0.0 0.0 13.421495397211535 0.0 74-75 0.0 0.0 0.0 14.006087040390941 0.0 76-77 0.0 0.0 0.0 14.59014285255002 0.0 78-79 0.0 0.0 0.0 15.2197443014371 0.0 80-81 0.0 0.0 0.0 15.92114710704832 0.0 82-83 0.0 0.0 0.0 16.582362586134835 0.0 84-85 0.0 0.0 0.0 17.172848370537867 0.0 86-87 0.0 0.0 0.0 17.74190091412772 0.0 88-89 0.0 0.0 0.0 18.33613751567306 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTTTACA 55 4.928177E-5 43.18182 1 GGTATCA 1285 0.0 42.87938 1 TTGGTAT 30 8.264063E-5 39.583336 16-17 CTATTAA 80 1.0670428E-5 35.625 1 ATGGGAT 55 0.002567545 34.545456 5 GGGTGAG 55 0.002567545 34.545456 8 GTAGACC 55 0.002567545 34.545456 3 ATGGGAG 60 0.0039325333 31.666668 5 CCTCGGG 30 0.003942549 31.666668 50-51 AGACCAG 60 0.0039325333 31.666668 5 AACCTCG 30 0.003942549 31.666668 48-49 TGTAGAC 60 0.0039325333 31.666668 2 GTATCAA 1880 0.0 30.571808 1 GGTGAGA 65 0.0058169514 29.230766 9 TAAAGGT 115 3.8147573E-6 28.913042 5 ATGGGTA 85 6.362816E-4 27.941175 5 CATGGGT 120 5.3124986E-6 27.708334 4 ATGGGGA 155 4.4930857E-8 27.580645 5 TGGGGAA 125 7.296152E-6 26.600002 6 ATGGGGT 165 8.238203E-8 25.909092 5 >>END_MODULE