FastQCFastQC Report
Thu 26 May 2016
SRR937942_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937942_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences93135
Sequences flagged as poor quality0
Sequence length101
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27782.982766951199871No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGT27032.902238685778708No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21822.3428356686530307No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19182.0593761743705374No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16101.728673431040962No Hit
ATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTT14361.5418478552638644RNA PCR Primer, Index 31 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCT8250.8858109196327911RNA PCR Primer, Index 31 (95% over 24bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6660.715090996939926No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5650.60664626617276No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3920.4208944006012777No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3640.39083051484404363No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3430.368282600526118No Hit
CATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCT3260.35002952703065443RNA PCR Primer, Index 31 (96% over 27bp)
TTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTC3010.3231867718902668No Hit
CACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTG2910.31244966983411177RNA PCR Primer, Index 38 (95% over 24bp)
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2900.31137595962849623No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1890.2029312288613303No Hit
TATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCT1740.18682557577709777RNA PCR Primer, Index 31 (95% over 21bp)
ATTTTAAACTTGCTAAAAAAACAACAAAATCAAAAAGTAAGTTTAGATTA1600.1717936328984807No Hit
AATCTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGCAAGTTTAAAATT1500.16105653084232566No Hit
TTCCAGAAGAGCTGTCCCTCTTTTGGCTATAATCTAAACTTACTTTTTGA1320.1417297471412466No Hit
CTTTACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTC1260.13528748590755355No Hit
ACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGC1240.13314006549632254RNA PCR Primer, Index 38 (96% over 25bp)
GTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1240.13314006549632254No Hit
CTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTC1170.125624094057014No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA1140.1224029634401675No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT1140.1224029634401675No Hit
GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAG1080.11596070220647448No Hit
CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT980.10522360015031942No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC970.10414988994470392No Hit
CTATTAAAGGTTTTTTCCGTTCCAGAAGAGCTGTCCCTCTTTTGGCTATA940.1009287593278574No Hit
CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA940.1009287593278574No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATAGCC200.002068076571.254
CGTTGAG459.5794915E-442.2222259
GGTATCA13000.040.1923071
ATGGGAG550.00256751734.5454567
CTTTAGG550.00256751734.5454562
GTATCAA19750.031.506331
ATGGGGT1102.6980379E-630.2272745
TGGGGTG953.4568613E-530.0000026
CTGAATT404.47935E-429.68750222-23
GCAGAGC650.00581688829.2307669
TGGGGAA1004.9050443E-528.56
ATGGGGA1354.1552266E-728.1481485
ATGGGTA856.362712E-427.9411775
CATGGGG3600.027.7083344
CAGGGTA350.00837383927.14285526-27
GGGACAG700.00835271127.1428555
GGGGTGA700.00835271127.1428557
AGACACC350.00837383927.14285592-93
ACCCACG458.919301E-426.38889192-93
TATCAAC23550.026.2208062
Produced by FastQC (version 0.11.5)