##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937942_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 93135 Sequences flagged as poor quality 0 Sequence length 101 %GC 37 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.26063241531111 33.0 31.0 34.0 31.0 34.0 2 32.4696945294465 34.0 31.0 34.0 31.0 34.0 3 32.35308960111666 34.0 31.0 34.0 30.0 34.0 4 35.98289579642454 37.0 35.0 37.0 35.0 37.0 5 35.98580555108176 37.0 35.0 37.0 35.0 37.0 6 36.09990873463252 37.0 35.0 37.0 35.0 37.0 7 36.02031459709025 37.0 35.0 37.0 35.0 37.0 8 36.06120148172008 37.0 35.0 37.0 35.0 37.0 9 37.66381059751973 39.0 37.0 39.0 35.0 39.0 10-11 37.67010253932463 39.0 37.0 39.0 35.0 39.0 12-13 37.59727814462876 39.0 37.0 39.0 35.0 39.0 14-15 38.632753529822295 40.0 38.0 41.0 34.0 41.0 16-17 38.57211574596016 40.0 38.0 41.0 34.5 41.0 18-19 38.71009287593279 40.0 38.0 41.0 35.0 41.0 20-21 38.80948622966661 40.0 38.0 41.0 35.0 41.0 22-23 38.67950824072582 40.0 38.0 41.0 35.0 41.0 24-25 38.53398829655876 40.0 38.0 41.0 35.0 41.0 26-27 38.20459547968004 40.0 37.0 41.0 34.0 41.0 28-29 37.94186932946798 40.0 36.5 41.0 33.5 41.0 30-31 37.3469211359854 39.0 35.0 41.0 33.0 41.0 32-33 36.66580769850218 38.0 35.0 41.0 33.0 41.0 34-35 36.153180866484135 38.0 35.0 41.0 31.5 41.0 36-37 35.65623557201911 38.0 35.0 40.5 31.0 41.0 38-39 35.14841896172223 38.0 35.0 40.5 29.5 41.0 40-41 34.769061040425186 38.0 35.0 40.0 27.0 41.0 42-43 34.13245289096473 37.5 35.0 40.5 22.0 41.0 44-45 33.32419605948354 37.0 34.5 40.0 7.5 41.0 46-47 32.22019648896763 36.5 33.0 40.0 2.0 41.0 48-49 31.211204165995596 36.0 32.5 40.0 2.0 41.0 50-51 30.36439576958179 35.0 31.0 40.0 2.0 41.0 52-53 29.68243946958716 35.0 29.0 40.0 2.0 41.0 54-55 29.131588554249205 35.0 26.5 40.0 2.0 41.0 56-57 28.47771514468245 35.0 23.5 39.5 2.0 41.0 58-59 27.765544639501798 35.0 17.0 39.0 2.0 41.0 60-61 26.969694529446503 35.0 2.0 39.0 2.0 41.0 62-63 26.214666881408707 35.0 2.0 38.0 2.0 40.0 64-65 25.42846405755087 34.0 2.0 38.0 2.0 40.0 66-67 24.50316207655554 33.5 2.0 37.0 2.0 40.0 68-69 23.623922263381115 33.0 2.0 36.5 2.0 39.0 70-71 22.913539485692812 33.0 2.0 36.0 2.0 39.0 72-73 22.4964245450153 32.0 2.0 35.0 2.0 38.5 74-75 22.078445267622268 31.5 2.0 35.0 2.0 37.5 76-77 21.091394212701992 29.5 2.0 34.5 2.0 36.0 78-79 21.577452085682076 31.0 2.0 35.0 2.0 36.0 80-81 21.438664304504215 31.0 2.0 35.0 2.0 36.0 82-83 21.137901970258227 31.0 2.0 35.0 2.0 36.0 84-85 20.83675310033822 31.0 2.0 35.0 2.0 35.0 86-87 20.472003006388576 30.0 2.0 35.0 2.0 35.0 88-89 20.074247060718314 29.5 2.0 35.0 2.0 35.0 90-91 19.75442100177162 29.0 2.0 34.0 2.0 35.0 92-93 19.37212648306222 28.0 2.0 34.0 2.0 35.0 94-95 18.908933268910722 26.5 2.0 34.0 2.0 35.0 96-97 18.437826810543832 25.0 2.0 34.0 2.0 35.0 98-99 17.86129811563859 23.5 2.0 34.0 2.0 35.0 100-101 15.71549900681806 2.0 2.0 31.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.08721743806268023 1101 2 -0.06746230504260353 1101 3 -0.17792868963275765 1101 4 0.12596170238323623 1101 5 0.041482093390058594 1101 6 0.07283622026674408 1101 7 -0.15152443122174475 1101 8 0.11274721882213612 1101 9 -0.22076090842714535 1101 10-11 -0.022255612302053862 1101 12-13 0.1349792095600435 1101 14-15 -0.028642749759647756 1101 16-17 -0.6148134693046288 1101 18-19 0.40494828574954767 1101 20-21 -0.29418412355379786 1101 22-23 -0.8759911930953024 1101 24-25 -0.6480960211290565 1101 26-27 -0.02367278071419321 1101 28-29 -0.1811253346922328 1101 30-31 0.3890610202180511 1101 32-33 0.1710849427579717 1101 34-35 -0.10658366482388004 1101 36-37 0.10315928524096307 1101 38-39 0.5481742348280534 1101 40-41 -0.04562699081503041 1101 42-43 -0.5355602843127301 1101 44-45 -0.7865352699787564 1101 46-47 -0.30231745920838904 1101 48-49 -0.48732018580646397 1101 50-51 -0.22949109524392242 1101 52-53 0.02119030020438828 1101 54-55 0.29268370616983574 1101 56-57 0.8763615102315505 1101 58-59 0.6996197076654767 1101 60-61 0.6252177430371404 1101 62-63 0.4727114820737661 1101 64-65 0.04464634944921286 1101 66-67 0.5168045758679689 1101 68-69 0.4141894403320059 1101 70-71 0.29263781839354763 1101 72-73 0.2777289181841738 1101 74-75 1.0177559254332813 1101 76-77 0.11363169823907882 1101 78-79 0.3151782649331132 1101 80-81 0.13660491210602643 1101 82-83 0.9654797880483734 1101 84-85 -0.2038149223359973 1101 86-87 -0.49487517160004657 1101 88-89 0.38918608520599207 1101 90-91 0.8887601138156036 1101 92-93 1.3281303204648474 1101 94-95 1.3597432153170814 1101 96-97 0.40138385323463766 1101 98-99 0.5365191489425811 1101 100-101 1.1530030367948694 1102 1 0.16018996934472796 1102 2 0.1374759665623344 1102 3 0.4255281004907019 1102 4 0.3526283690499028 1102 5 0.2380253032665962 1102 6 -0.27333661923943 1102 7 0.26674717371653145 1102 8 0.1645990706739866 1102 9 -0.24298313064937105 1102 10-11 -0.1602803036600733 1102 12-13 0.14954711079461447 1102 14-15 0.16518441073417733 1102 16-17 0.25086554304105135 1102 18-19 0.37186186599646476 1102 20-21 0.287050444347436 1102 22-23 0.009687819250373764 1102 24-25 0.40918792948823324 1102 26-27 0.48768524397716106 1102 28-29 -0.3485327420996427 1102 30-31 -0.7430377452140533 1102 32-33 -1.042989131316098 1102 34-35 -0.5080651463053627 1102 36-37 -0.5844950357466914 1102 38-39 -0.500220826900339 1102 40-41 -0.24463933649405334 1102 42-43 0.1770323082798555 1102 44-45 -0.6497451465219655 1102 46-47 -1.4265149900725902 1102 48-49 -2.3507769759299215 1102 50-51 -2.5912194903056474 1102 52-53 -2.1514022923882017 1102 54-55 -2.4157113555585568 1102 56-57 -2.247589107052402 1102 58-59 -1.6221086873962527 1102 60-61 -1.4661402816542157 1102 62-63 -2.036424320395369 1102 64-65 -1.5886869838841235 1102 66-67 -1.7350472759838844 1102 68-69 -1.4539587078161453 1102 70-71 -1.2641523050632415 1102 72-73 -1.2027649089763202 1102 74-75 -0.8321206177765959 1102 76-77 -0.6473559560819098 1102 78-79 -0.8680316116100961 1102 80-81 -1.2893210138198974 1102 82-83 -1.2248905823220007 1102 84-85 -0.8396173914717977 1102 86-87 -0.7403072703654772 1102 88-89 -0.8989620629421573 1102 90-91 -0.9791411207522991 1102 92-93 -0.9753264696586079 1102 94-95 -1.6607506118434117 1102 96-97 -1.4028136776295597 1102 98-99 -1.2533573942672973 1102 100-101 -1.1097130125878447 1103 1 0.4049564749807999 1103 2 0.41659566597189013 1103 3 0.3890278321063718 1103 4 0.4250921371658407 1103 5 0.2600328180277387 1103 6 0.31979274200587327 1103 7 0.053113249937673856 1103 8 0.3707182333148893 1103 9 0.529963729254014 1103 10-11 0.7919472862486785 1103 12-13 0.3497618182557005 1103 14-15 0.5844007285012225 1103 16-17 0.41243290750696104 1103 18-19 0.43654248864810796 1103 20-21 0.668424572098381 1103 22-23 0.8814001112525176 1103 24-25 1.1687155730738468 1103 26-27 1.332559103343776 1103 28-29 0.9284398826990667 1103 30-31 0.8702204405079073 1103 32-33 0.8209400152217441 1103 34-35 1.4734163351761254 1103 36-37 2.0698259519076245 1103 38-39 1.3441162638135609 1103 40-41 0.3908947483153966 1103 42-43 0.37429478815103323 1103 44-45 -0.6934917917178893 1103 46-47 -0.5985493432663631 1103 48-49 -0.2707984466760287 1103 50-51 0.2603639772198463 1103 52-53 0.4846685610739563 1103 54-55 1.1532634163147648 1103 56-57 1.1957818000866176 1103 58-59 1.301938548245186 1103 60-61 1.703478612602357 1103 62-63 1.3312622067114468 1103 64-65 1.3243564943767474 1103 66-67 2.1023118222447756 1103 68-69 3.2521604548247574 1103 70-71 2.6998841952051436 1103 72-73 2.6026564544160564 1103 74-75 2.7473211428245854 1103 76-77 2.3692838721521206 1103 78-79 2.6679318881215224 1103 80-81 2.5931266512364637 1103 82-83 2.5657696431208343 1103 84-85 3.0787937733161783 1103 86-87 3.1917914950666173 1103 88-89 3.756432462017589 1103 90-91 2.6829630123663293 1103 92-93 2.6263911900300663 1103 94-95 2.035975099375051 1103 96-97 2.5048621141042027 1103 98-99 2.26811335184113 1103 100-101 1.7730030367948704 1104 1 0.18460074375550306 1104 2 0.021174058593764755 1104 3 -0.04641353811760496 1104 4 0.06474958117111385 1104 5 0.08496694187490306 1104 6 0.22904834147886532 1104 7 0.1087785990812904 1104 8 0.03623206730698314 1104 9 0.2898451521789127 1104 10-11 0.18827469072825664 1104 12-13 0.3467973913782245 1104 14-15 -0.048339719456620855 1104 16-17 -0.049358923850086 1104 18-19 0.8484331342344049 1104 20-21 0.5259673915977174 1104 22-23 0.31931183720772083 1104 24-25 0.3051615546285191 1104 26-27 0.7849635829221739 1104 28-29 0.5475110289441218 1104 30-31 0.024288292945321643 1104 32-33 0.30760009427311985 1104 34-35 0.03402239578218769 1104 36-37 0.617174436756116 1104 38-39 0.18840150755532648 1104 40-41 0.7658124031243574 1104 42-43 0.40451547326302517 1104 44-45 0.0999041239606342 1104 46-47 0.15169769230675456 1104 48-49 -0.017092913079192584 1104 50-51 0.33035738960456484 1104 52-53 -0.16297636646227787 1104 54-55 -0.8691344756483446 1104 56-57 -0.8670475806775464 1104 58-59 -0.2895469590011892 1104 60-61 -0.6516761963567994 1104 62-63 -0.9839551845929009 1104 64-65 -1.3879294081265456 1104 66-67 -1.6294075453441543 1104 68-69 -1.5425529839104186 1104 70-71 -0.962665211909485 1104 72-73 -1.0990892636340064 1104 74-75 -1.1904258927485394 1104 76-77 -1.3017470896397079 1104 78-79 -1.305882341127493 1104 80-81 -1.348243572742458 1104 82-83 -1.210580818012236 1104 84-85 -1.3022997708208415 1104 86-87 -2.2135115352364103 1104 88-89 -1.8966472481273406 1104 90-91 -1.6763914013359091 1104 92-93 -0.7950514977169725 1104 94-95 -1.413135572561707 1104 96-97 -0.5594494800986958 1104 98-99 -0.7688596389362061 1104 100-101 -0.46563332684149294 1105 1 0.2962608228068859 1105 2 0.11949421669653049 1105 3 0.3419263828310122 1105 4 0.3562515574556997 1105 5 -0.047938196465018734 1105 6 0.11689419128124001 1105 7 0.23427267022753284 1105 8 0.13158779853227998 1105 9 -0.12221018378946269 1105 10-11 0.3372371413211397 1105 12-13 0.2845444269513422 1105 14-15 0.3434586995157147 1105 16-17 0.6714908785214533 1105 18-19 0.8187888654597018 1105 20-21 0.21914920977953756 1105 22-23 -0.03888974381994359 1105 24-25 0.27378803684195674 1105 26-27 0.7148054801553698 1105 28-29 0.8958311870468876 1105 30-31 0.9064523245658762 1105 32-33 0.31550523261304164 1105 34-35 0.5078366250311888 1105 36-37 0.6169274011829913 1105 38-39 0.7118698870019671 1105 40-41 0.6390831541124982 1105 42-43 0.4195846432234944 1105 44-45 0.3753487879922517 1105 46-47 0.21123326542928567 1105 48-49 0.6965928576717921 1105 50-51 1.235001658379268 1105 52-53 1.2093062422333745 1105 54-55 0.665944575735054 1105 56-57 0.5798397711011134 1105 58-59 1.0718660844770724 1105 60-61 1.3574641198487356 1105 62-63 1.2551752501897084 1105 64-65 1.018197074086892 1105 66-67 1.0153553005056466 1105 68-69 1.4315082809117143 1105 70-71 1.362927673466011 1105 72-73 0.8327289181841735 1105 74-75 1.0951472297811087 1105 76-77 1.5359505388187884 1105 78-79 2.1244536272519525 1105 80-81 1.6547208541350145 1105 82-83 1.4208421068889514 1105 84-85 1.9121271066495105 1105 86-87 1.2479509153564763 1105 88-89 1.372374491003093 1105 90-91 1.6521659109170521 1105 92-93 1.2894346682909337 1105 94-95 1.0414098819837463 1105 96-97 1.6063113894665229 1105 98-99 1.2644901634353332 1105 100-101 0.876263906360089 1106 1 0.12912664795882733 1106 2 -0.015849401816794284 1106 3 0.11927561144251086 1106 4 0.3526283690499028 1106 5 0.24320252349758675 1106 6 -0.05748636037841237 1106 7 0.13492718168148343 1106 8 0.15145689624149128 1106 9 -0.10678241380349363 1106 10-11 0.29451858124634356 1106 12-13 0.4311082418181087 1106 14-15 0.26813144378873943 1106 16-17 0.43206825112547875 1106 18-19 0.24322785564202576 1106 20-21 0.47183738182254586 1106 22-23 0.029600204754160586 1106 24-25 0.22738784983868499 1106 26-27 0.3232089397159186 1106 28-29 0.6717778911142105 1106 30-31 -0.03136908730883192 1106 32-33 0.4880741900698027 1106 34-35 0.7422335394771977 1106 36-37 0.4461700379291358 1106 38-39 0.29269036386030933 1106 40-41 0.18706118122797477 1106 42-43 1.00379455439694 1106 44-45 0.8934647300212362 1106 46-47 0.7602631859529012 1106 48-49 0.19741099698923392 1106 50-51 0.1479282595947886 1106 52-53 -0.8813903449569018 1106 54-55 -0.42925177770113265 1106 56-57 -0.8023481671878052 1106 58-59 -1.4904878192162414 1106 60-61 -0.984459676317698 1106 62-63 -0.13718099104451298 1106 64-65 -1.104816016142184 1106 66-67 -0.947711553164293 1106 68-69 -1.1283912048292848 1106 70-71 -0.5654266977354858 1106 72-73 -0.8218409742889463 1106 74-75 -0.8319214939215591 1106 76-77 -0.09518550606199838 1106 78-79 -0.15277872431419937 1106 80-81 -0.6967284212273022 1106 82-83 -1.3222621474354987 1106 84-85 -1.225320298680078 1106 86-87 -0.775950440417251 1106 88-89 -0.6164053126434688 1106 90-91 -0.7983366603779452 1106 92-93 -0.5400417225459044 1106 94-95 -0.6454180750055016 1106 96-97 -0.6407666843997681 1106 98-99 -0.6832657972939771 1106 100-101 -0.07108298471050567 1107 1 -0.0023194788790021903 1107 2 -0.12052352953239875 1107 3 0.084384235537307 1107 4 -0.08274578060996163 1107 5 -0.2779736889228701 1107 6 -0.5188644600053607 1107 7 -0.29057205026936117 1107 8 -0.3471167267560915 1107 9 -0.136407166930546 1107 10-11 -0.22742568032925448 1107 12-13 0.1343669646620853 1107 14-15 0.06251371282538543 1107 16-17 0.17559469396067584 1107 18-19 -0.04511974146132758 1107 20-21 -2.3854532250311422E-4 1107 22-23 0.16741016744891368 1107 24-25 0.3514277883947514 1107 26-27 0.42898028051029513 1107 28-29 0.13540527755265686 1107 30-31 -0.1578777552921551 1107 32-33 -0.8141531524801238 1107 34-35 -0.37182176006197665 1107 36-37 -0.3961604426501921 1107 38-39 -1.2412135202739876 1107 40-41 -1.751001140474898 1107 42-43 -2.5550160666256616 1107 44-45 -2.786807378822296 1107 46-47 -3.683065758528123 1107 48-49 -3.29364671641871 1107 50-51 -3.5924162653119467 1107 52-53 -3.4244559582990135 1107 54-55 -3.3250713958709817 1107 56-57 -3.269760938748046 1107 58-59 -3.19643471410323 1107 60-61 -3.5945101481193227 1107 62-63 -4.050281715205145 1107 64-65 -3.8761019498705167 1107 66-67 -4.208025356104823 1107 68-69 -3.32791940320541 1107 70-71 -3.4434166033751588 1107 72-73 -3.6749241430403146 1107 74-75 -3.5865297888524346 1107 76-77 -3.669905716726909 1107 78-79 -4.321420374522667 1107 80-81 -4.121898489254516 1107 82-83 -4.3052004840604745 1107 84-85 -4.084767303288377 1107 86-87 -3.8677323144571893 1107 88-89 -4.436188064453871 1107 90-91 -3.96396097461977 1107 92-93 -3.7192166183106625 1107 94-95 -3.338692158832581 1107 96-97 -3.2495005005068585 1107 98-99 -2.7135488782683037 1107 100-101 -1.96169084075615 1108 1 -0.34195428016793983 1108 2 -0.22535704188470618 1108 3 0.10908885422688996 1108 4 -0.4754418063886945 1108 5 -0.25606176625906585 1108 6 -0.12154974464553447 1108 7 -0.2048577645550793 1108 8 -0.7995334829322474 1108 9 -0.7084802066727605 1108 10-11 -0.37769420879327953 1108 12-13 -0.39554710622942935 1108 14-15 -0.6093445041456107 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56-57 -1.245689771819734 2316 58-59 -0.8082435401977683 2316 60-61 -0.8687993509799519 2316 62-63 0.27031831968060516 2316 64-65 -0.19125108644822575 2316 66-67 0.03560799467138409 2316 68-69 0.424958671101237 2316 70-71 0.0687061944619245 2316 72-73 -0.28158732113206497 2316 74-75 -0.04532099764364261 2316 76-77 0.635170159777541 2316 78-79 0.5256056153604618 2316 80-81 0.3374596129607319 2316 82-83 0.030095172663752834 2316 84-85 0.33037311185203677 2316 86-87 0.4652957685708934 2316 88-89 -0.3661985301786217 2316 90-91 -1.2488467237912353 2316 92-93 -0.5441773718428458 2316 94-95 -0.8549576393837768 2316 96-97 -1.3463084544576667 2316 98-99 -2.0392073467839147 2316 100-101 -2.0859713221794873 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 2.0 5 3.0 6 23.0 7 59.0 8 94.0 9 203.0 10 347.0 11 625.0 12 900.0 13 1328.0 14 1717.0 15 2060.0 16 2958.0 17 3753.0 18 3574.0 19 2437.0 20 2226.0 21 2262.0 22 2100.0 23 2276.0 24 2424.0 25 2489.0 26 1850.0 27 1164.0 28 1329.0 29 1432.0 30 1695.0 31 2018.0 32 2440.0 33 3063.0 34 4537.0 35 8129.0 36 8687.0 37 13119.0 38 9202.0 39 610.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.309872765340636 23.241531110753208 14.526225371772158 17.922370752133997 2 15.550472999817458 23.600597021335997 40.910994427085015 19.937935551761534 3 19.08233828708874 32.27922858860923 33.33906021819431 15.299372906107722 4 10.337681859666075 23.710742470607183 33.80254469318731 32.149030976539436 5 8.455558657418344 42.91987888418837 33.61608005669251 15.008482401700777 6 22.263381113437482 40.908358833950714 16.12820099855049 20.70005905406131 7 18.336822891501583 42.61985290170183 18.871530573898106 20.17179363289848 8 25.04106941536479 34.20303859988189 19.802437322166746 20.95345466258657 9 24.600848231062436 16.80678584849949 20.65174209480861 37.94062382562946 10-11 22.493155097439203 30.10737102056155 24.995973586728944 22.403500295270305 12-13 25.879637085950502 29.750362377194396 26.237182584420466 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14.132985378374169 30.194221155446733 38.29115453388533 17.381638932293765 40-41 14.223040821367587 30.361496246496195 38.76794862156734 16.64751431056888 42-43 15.814428007624775 29.686149220071417 38.587805729320486 15.911617042983329 44-45 14.32475530332723 30.753123473162557 37.936977519583785 16.985143703926422 46-47 16.021882918867846 29.233775716187775 36.14278658248507 18.601554782459306 48-49 15.598861867182048 28.966553927095077 36.06002040049391 19.37456380522897 50-51 14.288935416331134 28.933268910720994 36.85456595264938 19.92322972029849 52-53 14.992215601009287 29.21887582541472 37.508455467869226 18.28045310570677 54-55 15.095291780748376 28.811402802383633 35.16884092983304 20.92446448703495 56-57 16.53674773178719 30.2839963493853 33.00316744510657 20.176088473720945 58-59 14.787673806839535 31.47742524292693 34.6953347291566 19.03956622107693 60-61 16.041230471895634 33.396682235464645 31.18806034251356 19.37402695012616 62-63 14.529983357491814 34.473613571697 30.825146293015514 20.171256777795673 64-65 14.253502979545821 33.886830944328125 30.79991410318355 21.059751972942504 66-67 14.910076770279701 33.40527191710957 30.29473345144146 21.38991786116927 68-69 15.230042411553121 32.05347076823965 30.610404251892415 22.10608256831481 70-71 15.16132495839373 31.02002469533473 31.118806034251357 22.699844312020186 72-73 15.753476136790681 28.95152198421646 31.5305738981049 23.76442798088796 74-75 15.500080528265423 28.62242980619531 31.097868685241853 24.779620980297416 76-77 15.100660331776453 28.249315509743923 32.26499167874591 24.38503247973372 78-79 15.36425618725506 27.870832662264455 31.856444945509203 24.908466204971276 80-81 15.224137005422236 27.3130402104472 31.957910559939872 25.50491222419069 82-83 14.835453910989424 27.493423524990607 31.569227465507062 26.101895098512912 84-85 15.145088986121923 26.900383861702416 31.07722867956943 26.87729847260623 86-87 15.210260429808503 27.03348383252178 30.80705860878622 26.949197128883494 88-89 15.311644387179902 26.71552048102217 29.998926289794387 27.973908842003542 90-91 15.48798230497082 25.56867292649798 30.72256492024889 28.220779848282305 92-93 15.335802866806251 25.41633113222741 30.718312127556775 28.52955387340957 94-95 15.210715627852043 25.267622268749662 30.494980404788745 29.026681698609547 96-97 15.282117356525474 25.244537499328928 30.117034412412092 29.356310731733505 98-99 15.448005583293071 25.00778439899071 29.816395554839747 29.727814462876466 100-101 15.462876961650176 24.778936856742494 30.305649659882206 29.452536521725126 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1556.0 1 903.5 2 319.5 3 365.0 4 240.0 5 194.5 6 390.5 7 655.0 8 849.0 9 856.0 10 641.0 11 451.5 12 400.5 13 387.0 14 416.0 15 445.5 16 461.5 17 512.5 18 550.0 19 631.5 20 699.5 21 723.0 22 819.5 23 919.0 24 996.0 25 1127.0 26 1367.0 27 1694.5 28 1888.5 29 2011.5 30 2178.5 31 2300.0 32 2461.0 33 2574.5 34 2706.5 35 2816.0 36 2920.0 37 2970.0 38 2644.0 39 2274.5 40 2153.0 41 2132.5 42 2163.5 43 2292.5 44 2409.5 45 2418.0 46 2397.5 47 2390.5 48 2522.5 49 2584.0 50 2676.5 51 2848.0 52 2544.5 53 1851.5 54 1245.5 55 940.5 56 822.0 57 736.5 58 698.5 59 667.5 60 616.0 61 583.0 62 575.0 63 558.0 64 494.0 65 441.0 66 416.0 67 389.5 68 349.0 69 307.0 70 291.5 71 270.0 72 206.0 73 158.0 74 130.0 75 96.5 76 71.0 77 58.5 78 39.5 79 19.0 80 12.0 81 7.5 82 4.0 83 4.0 84 3.0 85 1.5 86 1.0 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.006442261233693027 3 0.00751597143930853 4 0.0 5 0.0010737102056155043 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 5.368551028077521E-4 26-27 0.020937349009502334 28-29 0.0010737102056155043 30-31 0.0 32-33 0.0032211306168465135 34-35 0.015568797981424813 36-37 0.008589681644924034 38-39 0.020937349009502334 40-41 0.023621624523541097 42-43 0.018789928598271326 44-45 0.010200246953347291 46-47 0.0032211306168465135 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.002684275514038761 86-87 5.368551028077521E-4 88-89 0.0 90-91 0.0016105653084232568 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.005905406130885274 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 93135.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.46974821495678 #Duplication Level Percentage of deduplicated Percentage of total 1 83.91660461653015 42.35249906050357 2 8.39697904478247 8.475868363128791 3 2.772045527071588 4.197133193751006 4 1.2445484522923094 2.5124818811402805 5 0.8552281672162536 2.158157513287164 6 0.519093713434741 1.5719117410210985 7 0.3595362195511116 1.2701991732431417 8 0.278693755983406 1.1252482954850487 9 0.18083182640144665 0.8213883072958608 >10 1.3296457823635783 12.449669834111774 >50 0.08722476332305074 3.070811188060343 >100 0.04042123178385278 4.229344499919472 >500 0.006382299755345176 2.207548182745477 >1k 0.012764599510690353 13.557738766306974 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2778 2.982766951199871 No Hit CTTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGT 2703 2.902238685778708 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2182 2.3428356686530307 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1918 2.0593761743705374 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1610 1.728673431040962 No Hit ATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTT 1436 1.5418478552638644 RNA PCR Primer, Index 31 (95% over 22bp) ACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCT 825 0.8858109196327911 RNA PCR Primer, Index 31 (95% over 24bp) ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 666 0.715090996939926 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 565 0.60664626617276 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 392 0.4208944006012777 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 364 0.39083051484404363 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 343 0.368282600526118 No Hit CATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCT 326 0.35002952703065443 RNA PCR Primer, Index 31 (96% over 27bp) TTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTC 301 0.3231867718902668 No Hit CACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTG 291 0.31244966983411177 RNA PCR Primer, Index 38 (95% over 24bp) ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 290 0.31137595962849623 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 189 0.2029312288613303 No Hit TATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCT 174 0.18682557577709777 RNA PCR Primer, Index 31 (95% over 21bp) ATTTTAAACTTGCTAAAAAAACAACAAAATCAAAAAGTAAGTTTAGATTA 160 0.1717936328984807 No Hit AATCTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGCAAGTTTAAAATT 150 0.16105653084232566 No Hit TTCCAGAAGAGCTGTCCCTCTTTTGGCTATAATCTAAACTTACTTTTTGA 132 0.1417297471412466 No Hit CTTTACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTC 126 0.13528748590755355 No Hit ACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGC 124 0.13314006549632254 RNA PCR Primer, Index 38 (96% over 25bp) GTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 124 0.13314006549632254 No Hit CTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTC 117 0.125624094057014 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 114 0.1224029634401675 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 114 0.1224029634401675 No Hit GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAG 108 0.11596070220647448 No Hit CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT 98 0.10522360015031942 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 97 0.10414988994470392 No Hit CTATTAAAGGTTTTTTCCGTTCCAGAAGAGCTGTCCCTCTTTTGGCTATA 94 0.1009287593278574 No Hit CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA 94 0.1009287593278574 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0010737102056155043 0.0 10-11 0.0 0.0 0.0 0.0010737102056155043 0.0 12-13 0.0 0.0 0.0 0.0021474204112310086 0.0 14-15 0.0 0.0 0.0 0.0021474204112310086 0.0 16-17 0.0 0.0 0.0 0.0021474204112310086 0.0 18-19 0.0 0.0 0.0 0.002684275514038761 0.0 20-21 0.0 0.0 0.0 0.003221130616846513 0.0 22-23 0.0 0.0 0.0 0.009663391850539538 0.0 24-25 0.0 0.0 0.0 0.028453320448810868 0.0 26-27 0.0 0.0 0.0 0.0730122939818543 0.0 28-29 0.0 0.0 0.0 0.1895098512911365 0.0 30-31 0.0 0.0 0.0 0.47028507005959097 0.0 32-33 0.0 0.0 0.0 0.8954743114833306 0.0 34-35 0.0 0.0 0.0 1.4575616041230472 0.0 36-37 0.0 0.0 0.0 2.1356095989692383 0.0 38-39 0.0 0.0 5.368551028077521E-4 3.0181993879851827 0.0 40-41 0.0 0.0 0.0010737102056155043 3.992591399581253 0.0 42-43 0.0 0.0 0.0010737102056155043 4.69318730874537 0.0 44-45 0.0 0.0 0.0010737102056155043 5.343855693348365 0.0 46-47 0.0 0.0 0.0010737102056155043 5.998818918773823 0.0 48-49 0.0 0.0 0.0010737102056155043 6.647339882965587 0.0 50-51 0.0 0.0 0.0010737102056155043 7.309819079830353 0.0 52-53 0.0 0.0 0.0010737102056155043 7.839158211198797 0.0 54-55 0.0 0.0 0.0010737102056155043 8.350781124174585 0.0 56-57 0.0 0.0 0.0010737102056155043 8.938100606646266 0.0 58-59 0.0 0.0 0.0010737102056155043 9.50770387072529 0.0 60-61 0.0 0.0 0.0010737102056155043 10.103076179739087 0.0 62-63 0.0 0.0 0.0010737102056155043 10.630267890696302 0.0 64-65 0.0 0.0 0.0010737102056155043 11.136522252644012 0.0 66-67 0.0 0.0 0.0010737102056155043 11.697535835078114 0.0 68-69 0.0 0.0 0.0010737102056155043 12.313308637998604 0.0 70-71 0.0 0.0 0.0010737102056155043 13.004778010414988 0.0 72-73 0.0 0.0 0.0010737102056155043 13.602834594942824 0.0 74-75 0.0 0.0 0.0010737102056155043 14.199817469265046 0.0 76-77 0.0 0.0 0.0010737102056155043 14.801632039512537 0.0 78-79 0.0 0.0 0.0010737102056155043 15.403446609760024 0.0 80-81 0.0 0.0 0.0010737102056155043 16.022440543297364 0.0 82-83 0.0 0.0 0.0010737102056155043 16.658076985021744 0.0 84-85 0.0 0.0 0.0010737102056155043 17.260428410372043 0.0 86-87 0.0 0.0 0.0010737102056155043 17.809631180544372 0.0 88-89 0.0 0.0 0.0010737102056155043 18.36849734256724 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GATAGCC 20 0.0020680765 71.25 4 CGTTGAG 45 9.5794915E-4 42.222225 9 GGTATCA 1300 0.0 40.192307 1 ATGGGAG 55 0.002567517 34.545456 7 CTTTAGG 55 0.002567517 34.545456 2 GTATCAA 1975 0.0 31.50633 1 ATGGGGT 110 2.6980379E-6 30.227274 5 TGGGGTG 95 3.4568613E-5 30.000002 6 CTGAATT 40 4.47935E-4 29.687502 22-23 GCAGAGC 65 0.005816888 29.230766 9 TGGGGAA 100 4.9050443E-5 28.5 6 ATGGGGA 135 4.1552266E-7 28.148148 5 ATGGGTA 85 6.362712E-4 27.941177 5 CATGGGG 360 0.0 27.708334 4 CAGGGTA 35 0.008373839 27.142855 26-27 GGGACAG 70 0.008352711 27.142855 5 GGGGTGA 70 0.008352711 27.142855 7 AGACACC 35 0.008373839 27.142855 92-93 ACCCACG 45 8.919301E-4 26.388891 92-93 TATCAAC 2355 0.0 26.220806 2 >>END_MODULE