##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937941_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 93531 Sequences flagged as poor quality 0 Sequence length 101 %GC 37 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.2816499342464 33.0 31.0 34.0 31.0 34.0 2 32.482802493291 34.0 31.0 34.0 31.0 34.0 3 32.46608076466626 34.0 31.0 34.0 30.0 34.0 4 36.017598443296876 37.0 35.0 37.0 35.0 37.0 5 36.019886454758314 37.0 35.0 37.0 35.0 37.0 6 36.11675273438753 37.0 36.0 37.0 35.0 37.0 7 36.04038233313019 37.0 35.0 37.0 35.0 37.0 8 36.083758326116474 37.0 35.0 37.0 35.0 37.0 9 37.667767905828015 39.0 37.0 39.0 35.0 39.0 10-11 37.69445424511659 39.0 37.5 39.0 35.0 39.0 12-13 37.63615271941923 39.0 37.0 39.0 35.0 39.0 14-15 38.70011012391613 40.0 38.0 41.0 34.5 41.0 16-17 38.65511969293603 40.0 38.0 41.0 34.5 41.0 18-19 38.80632089895329 40.0 38.0 41.0 35.0 41.0 20-21 38.88083630026408 40.0 38.0 41.0 35.0 41.0 22-23 38.67540708428221 40.0 38.0 41.0 35.0 41.0 24-25 38.50146475500102 40.0 38.0 41.0 34.5 41.0 26-27 38.23433941687783 40.0 37.0 41.0 34.0 41.0 28-29 38.00450118142648 40.0 36.5 41.0 34.0 41.0 30-31 37.40328874918476 39.0 35.5 41.0 33.0 41.0 32-33 36.752675583496384 38.5 35.0 41.0 33.0 41.0 34-35 36.213089777720754 38.0 35.0 41.0 32.0 41.0 36-37 35.76327100105847 38.0 35.0 41.0 31.0 41.0 38-39 35.22061669393035 38.0 35.0 41.0 29.5 41.0 40-41 34.89372507510879 38.0 35.0 41.0 27.5 41.0 42-43 34.30279265698004 38.0 35.0 41.0 22.5 41.0 44-45 33.498973602335056 37.0 34.5 41.0 8.5 41.0 46-47 32.408538345575266 37.0 33.0 40.0 2.0 41.0 48-49 31.414899872769457 36.5 33.0 40.0 2.0 41.0 50-51 30.55206829821129 35.5 31.0 40.0 2.0 41.0 52-53 29.884979311672065 35.0 29.5 40.0 2.0 41.0 54-55 29.386379916819024 35.0 27.5 40.0 2.0 41.0 56-57 28.763752124963915 35.0 25.0 40.0 2.0 41.0 58-59 28.10471394510911 35.0 20.5 39.5 2.0 41.0 60-61 27.36603906726112 35.0 4.5 39.0 2.0 41.0 62-63 26.657749836952455 35.0 2.0 39.0 2.0 41.0 64-65 25.889266660251682 35.0 2.0 38.5 2.0 40.5 66-67 24.974500433011514 34.5 2.0 37.5 2.0 40.0 68-69 24.098111855962195 34.0 2.0 37.0 2.0 40.0 70-71 23.46624113930141 33.5 2.0 36.5 2.0 39.0 72-73 23.059493643818627 33.0 2.0 36.0 2.0 39.0 74-75 22.686392746789835 33.0 2.0 35.0 2.0 38.0 76-77 21.643465802781968 30.5 2.0 34.5 2.0 36.5 78-79 22.116100544204595 32.5 2.0 35.0 2.0 37.0 80-81 21.951631010039453 33.0 2.0 35.0 2.0 36.5 82-83 21.640242272615495 32.0 2.0 35.0 2.0 36.0 84-85 21.373207813452225 32.0 2.0 35.0 2.0 35.5 86-87 21.05001015706023 31.0 2.0 35.0 2.0 35.0 88-89 20.688654029145418 31.0 2.0 35.0 2.0 35.0 90-91 20.425415103013975 30.5 2.0 35.0 2.0 35.0 92-93 20.14829307929991 30.0 2.0 35.0 2.0 35.0 94-95 19.91987683228021 29.5 2.0 35.0 2.0 35.0 96-97 19.54783975366456 29.0 2.0 35.0 2.0 35.0 98-99 19.12802172541724 29.0 2.0 34.0 2.0 35.0 100-101 17.707326982497783 22.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.048404622729734115 1101 2 0.09725208718990785 1101 3 -0.33401376447945097 1101 4 -0.41689123478555246 1101 5 0.0717885710413455 1101 6 0.23467404871507824 1101 7 0.014852070794560746 1101 8 0.3059130815426414 1101 9 -0.16030925395900653 1101 10-11 0.07472411198086348 1101 12-13 0.06787353779423455 1101 14-15 -0.1036535629763975 1101 16-17 0.18030878010693385 1101 18-19 0.10000310303627202 1101 20-21 -0.1087145122192652 1101 22-23 -0.2777213739716231 1101 24-25 -0.024398300041163168 1101 26-27 -0.36505772070190545 1101 28-29 -0.006653903899916713 1101 30-31 0.3186603867029163 1101 32-33 -0.24228327150431994 1101 34-35 -0.26043682883334185 1101 36-37 -0.5225340016214446 1101 38-39 0.0958737493863211 1101 40-41 -0.3935563979062664 1101 42-43 -0.2779188107490853 1101 44-45 0.06294646986402341 1101 46-47 -0.12503126216846283 1101 48-49 0.35297655931257665 1101 50-51 0.9243649581553406 1101 52-53 0.9919719712338058 1101 54-55 0.8556121246004018 1101 56-57 0.8773644133115468 1101 58-59 1.5212224365441074 1101 60-61 0.780840480204958 1101 62-63 1.1751096607492073 1101 64-65 -0.1574708284568871 1101 66-67 0.3728179475583424 1101 68-69 0.862515900381517 1101 70-71 0.3434101946703585 1101 72-73 -0.255085443521498 1101 74-75 0.3411103740652486 1101 76-77 0.47690232830517587 1101 78-79 0.435623193422483 1101 80-81 0.4533069551116782 1101 82-83 0.9164375534136546 1101 84-85 1.2139398269646904 1101 86-87 1.2204666697339057 1101 88-89 0.8835071867321638 1101 90-91 1.4368618013141763 1101 92-93 1.3241273315416642 1101 94-95 1.2149579873193517 1101 96-97 1.0938755284230908 1101 98-99 1.0855108595111673 1101 100-101 1.9211557786710038 1102 1 0.35708374848203306 1102 2 0.4007047321059716 1102 3 0.27764287156742995 1102 4 0.14265897110611547 1102 5 0.11185825745248934 1102 6 -0.09316884010342363 1102 7 0.30278365140907226 1102 8 -0.13247779587262443 1102 9 0.6852719940612602 1102 10-11 0.3251992466023452 1102 12-13 0.25032523244104965 1102 14-15 -0.2510244847375631 1102 16-17 0.16423339208032672 1102 18-19 0.3828665810216947 1102 20-21 0.4019760167639461 1102 22-23 0.36687792916426787 1102 24-25 0.3646577658441643 1102 26-27 0.14499929545267776 1102 28-29 0.06374520918211601 1102 30-31 0.24438100754549197 1102 32-33 0.07692800502403685 1102 34-35 -0.039736797157701176 1102 36-37 0.43605009720655374 1102 38-39 0.5947651019362041 1102 40-41 0.5145842419416908 1102 42-43 -0.21987319782542158 1102 44-45 0.24999113251843852 1102 46-47 0.2542845439766097 1102 48-49 -0.38752074825790217 1102 50-51 -0.6612384628139338 1102 52-53 -0.36699223529137726 1102 54-55 -0.2731493578196229 1102 56-57 0.4130786990258315 1102 58-59 0.7450108432593439 1102 60-61 0.9335962610095194 1102 62-63 0.956230978455892 1102 64-65 1.1567515345459647 1102 66-67 1.4724378398611613 1102 68-69 2.3066875823580766 1102 70-71 2.4308349650219547 1102 72-73 2.3266377113723884 1102 74-75 2.356869005677538 1102 76-77 2.28962009834002 1102 78-79 2.921448343881778 1102 80-81 2.126255957328972 1102 82-83 2.3900675819217305 1102 84-85 2.346898331874417 1102 86-87 2.432614278222978 1102 88-89 2.2428673387752447 1102 90-91 1.8743815985900696 1102 92-93 1.367206203888827 1102 94-95 1.219234198912634 1102 96-97 1.6953167701082315 1102 98-99 2.0256438972051782 1102 100-101 1.8456093738563055 1103 1 0.16074228506739985 1103 2 0.2738754638132832 1103 3 0.2846550666893819 1103 4 -0.07776785816217568 1103 5 0.07893142818419818 1103 6 0.25805067209169863 1103 7 0.18692999287248568 1103 8 0.09130269193224905 1103 9 0.33222321357345663 1103 10-11 0.14501631977307028 1103 12-13 0.31633133000202207 1103 14-15 0.1357133201404892 1103 16-17 0.5041724164705741 1103 18-19 0.2577141419973117 1103 20-21 -0.26463983689458814 1103 22-23 0.1374571974569463 1103 24-25 -0.04387881952168726 1103 26-27 0.10612734423316539 1103 28-29 0.4291415506455394 1103 30-31 0.33797856852110186 1103 32-33 -0.125968336439378 1103 34-35 0.35279369064717514 1103 36-37 -0.07050478084222078 1103 38-39 -0.14730806879549618 1103 40-41 0.44995009560022936 1103 42-43 0.34902924119896994 1103 44-45 1.1804789373964866 1103 46-47 1.5957479586107581 1103 48-49 1.1900707151567325 1103 50-51 1.6454688542592386 1103 52-53 0.036208984220817086 1103 54-55 -1.718576187087912 1103 56-57 -0.8744213009741699 1103 58-59 -0.4273976933260215 1103 60-61 0.3297852853997618 1103 62-63 0.8354992711388185 1103 64-65 1.0332759247898657 1103 66-67 0.8628341813245761 1103 68-69 0.10943148479710274 1103 70-71 0.7959264284365908 1103 72-73 1.1789242967382414 1103 74-75 1.8308019325068052 1103 76-77 1.4054737568766065 1103 78-79 0.8906556609549483 1103 80-81 0.4146705914753177 1103 82-83 0.5402200209461192 1103 84-85 0.8194593074841734 1103 86-87 1.7246874489546862 1103 88-89 1.095763680238658 1103 90-91 0.3309364766388505 1103 92-93 -0.03812916196482874 1103 94-95 0.02258785744922065 1103 96-97 0.038761892059454794 1103 98-99 0.5036926776929853 1103 100-101 0.5134447397099642 1104 1 0.12185339617850843 1104 2 0.07387546381328747 1104 3 0.2860439555782719 1104 4 -0.018045635939948568 1104 5 0.27337587262864105 1104 6 0.16916178320281006 1104 7 0.13692999287248853 1104 8 0.38296935859892045 1104 9 0.5183343246845666 1104 10-11 0.27696076421751314 1104 12-13 0.3378591077797992 1104 14-15 0.22390776458492923 1104 16-17 0.21667241647057978 1104 18-19 0.633408586441746 1104 20-21 0.694387940883189 1104 22-23 0.4159294196791663 1104 24-25 0.47834340270053843 1104 26-27 0.8720995664553897 1104 28-29 0.7590026617566465 1104 30-31 0.1671452351877747 1104 32-33 0.342781663560622 1104 34-35 0.38682146842495513 1104 36-37 0.33157855249110924 1104 38-39 0.3610252645378367 1104 40-41 0.2777278733780051 1104 42-43 -0.06485964768992147 1104 44-45 -0.1972988403812863 1104 46-47 -1.176474263611464 1104 48-49 -1.3467348403988204 1104 50-51 -1.501753367962987 1104 52-53 -1.9672632380014043 1104 54-55 -2.622742853754577 1104 56-57 -3.131365745418613 1104 58-59 -2.48086991554824 1104 60-61 -2.658409159044684 1104 62-63 -2.347834062194515 1104 64-65 -2.6875574085434657 1104 66-67 -2.4878602631198667 1104 68-69 -2.325290737425121 1104 70-71 -2.106156904896743 1104 72-73 -1.76482570326176 1104 74-75 -1.9504480674931948 1104 76-77 -2.1889706875678385 1104 78-79 -1.9857332279339381 1104 80-81 -1.7047738529691294 1104 82-83 -1.3049188679427708 1104 84-85 -1.1777629147380466 1104 86-87 -1.2225347732675367 1104 88-89 -0.8229863197613447 1104 90-91 -0.9211468566944809 1104 92-93 -1.0471569397426101 1104 94-95 -1.2260232536618894 1104 96-97 -1.0147103301627673 1104 98-99 -0.8018628778625718 1104 100-101 -0.945583038067813 1105 1 0.48602318394380006 1105 2 0.22668445257733083 1105 3 0.14884045994780593 1105 4 -0.2312790941172338 1105 5 0.266010079869595 1105 6 0.09400572827147613 1105 7 0.004345723209567609 1105 8 0.06237010766258777 1105 9 0.08250411244986111 1105 10-11 0.1813927242674609 1105 12-13 0.11850829629415927 1105 14-15 -0.04637937648872992 1105 16-17 0.15936904568405907 1105 18-19 0.6108742543568582 1105 20-21 0.4991944327683342 1105 22-23 0.8330330401535733 1105 24-25 0.8943234276693275 1105 26-27 0.7392031869297924 1105 28-29 0.37485784278037215 1105 30-31 0.34317519773458116 1105 32-33 0.4792985174931985 1105 34-35 0.5511785221078469 1105 36-37 0.821840724775754 1105 38-39 0.5080290098561875 1105 40-41 0.8583770618923623 1105 42-43 0.7009955333338027 1105 44-45 0.030478937396487993 1105 46-47 1.0283322282736833 1105 48-49 0.9076971196511145 1105 50-51 0.14069357336035893 1105 52-53 0.44877920893991785 1105 54-55 0.5607777454963561 1105 56-57 -0.15714602007529166 1105 58-59 -0.597411738269841 1105 60-61 0.03547348764695357 1105 62-63 -0.11899511088365244 1105 64-65 -0.010965648243843162 1105 66-67 0.48860665323468666 1105 68-69 0.9525494623251909 1105 70-71 1.08841238349277 1105 72-73 0.9402304765135234 1105 74-75 0.8995519325068067 1105 76-77 1.0908670153035729 1105 78-79 1.0660770092695557 1105 80-81 0.9211312656326207 1105 82-83 0.4907115939798281 1105 84-85 0.031537959169565255 1105 86-87 0.2581144152468191 1105 88-89 0.19723839933978127 1105 90-91 0.690838162032108 1105 92-93 0.959834321181237 1105 94-95 1.422166509134616 1105 96-97 0.7833545886886668 1105 98-99 1.4879623406143345 1105 100-101 2.01140822285603 1106 1 0.07437864870376387 1106 2 -0.13976089982308082 1106 3 -0.03693584240152603 1106 4 -0.040267858162174264 1106 5 0.16756779182056647 1106 6 0.17623249027352017 1106 7 0.20965726559975906 1106 8 0.22312087375043177 1106 9 -0.03368587733563544 1106 10-11 0.18592541068216661 1106 12-13 -0.12855503363434195 1106 14-15 0.04650877468593961 1106 16-17 -0.035600310802152535 1106 18-19 0.18441868745185275 1106 20-21 0.379110163105409 1106 22-23 0.3846162883660398 1106 24-25 0.21748481684195298 1106 26-27 0.49533188968771213 1106 28-29 0.3342551870091768 1106 30-31 0.3186603867029234 1106 32-33 0.1870998453788033 1106 34-35 -0.0989108548073716 1106 36-37 0.2664270373395965 1106 38-39 -0.284808068795499 1106 40-41 -0.0364135407634123 1106 42-43 -0.3111980315283063 1106 44-45 -1.0036119716944185 1106 46-47 -0.915615677752875 1106 48-49 -0.7866338302978129 1106 50-51 -1.2363493275589441 1106 52-53 -1.2961773794155462 1106 54-55 -1.4844852779970026 1106 56-57 -1.4562394827923484 1106 58-59 -1.9097840569623834 1106 60-61 -1.1582828964184202 1106 62-63 -1.0610916379520887 1106 64-65 -1.3417240752101343 1106 66-67 -0.4900067277663318 1106 68-69 -1.3598866970210786 1106 70-71 -1.2012326624724992 1106 72-73 -0.9352802487163068 1106 74-75 -1.4186298856750135 1106 76-77 -1.3956626067597604 1106 78-79 -1.1877534299541388 1106 80-81 -1.5239657721610484 1106 82-83 -1.3148936154175175 1106 84-85 -0.8680406925158266 1106 86-87 -0.6934943692271354 1106 88-89 -0.40139541067043183 1106 90-91 -0.8253135233611495 1106 92-93 -0.6125609801466503 1106 94-95 -1.1024121425507794 1106 96-97 -0.639079017031456 1106 98-99 0.11960176860207739 1106 100-101 0.44469473970996276 1107 1 -0.22803322513668434 1107 2 0.05550811687451329 1107 3 -0.03345717820857885 1107 4 -0.1933290826519709 1107 5 -0.12208897997906831 1107 6 -0.35623504219401525 1107 7 0.02876672756636367 1107 8 -0.12451363459835818 1107 9 0.20518239724692933 1107 10-11 -0.20727959859427614 1107 12-13 -0.24195948632451092 1107 14-15 -0.3531897410840017 1107 16-17 -0.45883778761105276 1107 18-19 -0.5089950416761582 1107 20-21 -0.566042898119079 1107 22-23 -0.512415251522647 1107 24-25 -0.42142983992984995 1107 26-27 -0.5144083700525499 1107 28-29 0.004013999625129827 1107 30-31 -0.18829694168297806 1107 32-33 -0.3174224180720344 1107 34-35 -0.322588962414045 1107 36-37 -0.45991804614834564 1107 38-39 0.660344992428989 1107 40-41 0.5846439731512518 1107 42-43 1.075049649362235 1107 44-45 0.1376217945393492 1107 46-47 -0.9144561230218926 1107 48-49 -0.6901588766799982 1107 50-51 -0.42442910492443886 1107 52-53 -0.1430001994526542 1107 54-55 0.6765768741365754 1107 56-57 1.208997066372767 1107 58-59 1.1209441434086713 1107 60-61 1.1370556935630276 1107 62-63 0.9895809037918823 1107 64-65 1.6978167411163945 1107 66-67 1.5810739772429407 1107 68-69 2.6867274031644506 1107 70-71 2.1230947957835298 1107 72-73 2.6331334804117112 1107 74-75 2.0373070345476236 1107 76-77 2.3034329405500777 1107 78-79 2.3980536201386258 1107 80-81 2.8804869180059285 1107 82-83 3.1747863474767293 1107 84-85 3.322010327892336 1107 86-87 2.7751976530363187 1107 88-89 3.6372177618713124 1107 90-91 3.459125252149054 1107 92-93 2.738784103341292 1107 94-95 2.5850878574492206 1107 96-97 1.824858830834966 1107 98-99 1.7761416572848212 1107 100-101 1.7189294335875154 1108 1 0.04737594843373216 1108 2 0.1372418004469509 1108 3 0.08131348253096604 1108 4 0.38547471609525275 1108 5 0.18635717075845548 1108 6 0.11547641466595593 1108 7 0.016137913664564962 1108 8 0.2628373453975996 1108 9 0.13890638189028692 1108 10-11 -0.009562888147719661 1108 12-13 0.033348656734695226 1108 14-15 0.3438197557840468 1108 16-17 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164.0 10 331.0 11 549.0 12 875.0 13 1321.0 14 1649.0 15 2141.0 16 2788.0 17 3689.0 18 3624.0 19 2509.0 20 2191.0 21 2208.0 22 2106.0 23 2208.0 24 2304.0 25 2426.0 26 1769.0 27 1123.0 28 1154.0 29 1287.0 30 1568.0 31 1812.0 32 2120.0 33 2730.0 34 3852.0 35 7370.0 36 8237.0 37 12724.0 38 12922.0 39 1584.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.650436753589716 23.040489249553627 14.58233099186366 17.726743004992997 2 15.70111845341203 23.521738200637284 40.702722354098505 20.074420991852186 3 18.637797380379578 32.3763699545576 33.77064955894146 15.215183106121358 4 10.405106328383102 23.739722658797618 33.98017769509574 31.874993317723536 5 8.420737509488832 42.3784627556639 33.92030449797394 15.280495236873337 6 22.289935957062365 40.77150891148389 16.121927489281628 20.816627642172115 7 18.341512439725864 42.42978263891116 18.880371213822155 20.348333707540817 8 25.05827007377312 34.101357853095266 20.00534587832781 20.835026194803806 9 24.94573991510836 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16.325057736720556 34-35 14.429092386829076 30.983648632751926 38.25781475579891 16.32944422462009 36-37 13.912769187998547 31.561023074784543 38.65935287953637 15.866854857680545 38-39 14.15035318400325 30.24014373332335 38.46256677343286 17.146936309240534 40-41 14.153263752245701 30.350650183933613 38.8789674052528 16.617118658567883 42-43 15.599837462707313 29.528855716073014 38.964573286140485 15.906733535079184 44-45 14.262954171211051 30.63022604307008 38.282436218216034 16.824383567502835 46-47 16.00271567715517 29.190321925350954 36.36815601244508 18.438806385048807 48-49 15.497535576439898 28.754102917749197 36.28850327698838 19.45985822882253 50-51 14.33802696432199 29.040104350429267 36.89311565149523 19.728753033753517 52-53 15.281564401107653 29.249660540355606 37.46779142744117 18.000983631095576 54-55 15.19122002330778 28.875987640461453 35.479145951609624 20.45364638462114 56-57 16.641006725043034 30.04511873068822 33.42421229325036 19.88966225101838 58-59 14.788679689087042 31.612513498198457 34.805037901872105 18.793768910842395 60-61 16.015545647966984 33.14890250291347 31.50933914958677 19.326212699532775 62-63 14.291518320129153 34.49498027391988 31.185916968705563 20.027584437245405 64-65 14.22416097336712 34.029359249874375 31.231891030781238 20.51458874597727 66-67 14.892923201933048 33.24191979129914 30.732056751237558 21.133100255530252 68-69 14.90102159188714 32.00720620546239 31.038003645869527 22.05376855678094 70-71 15.199238755065164 30.885481818862193 31.423271428724163 22.492007997348473 72-73 15.543509638515573 29.30098042360287 31.682009173429133 23.473500764452425 74-75 15.38206583913355 28.794196576536123 31.277330510739752 24.546407073590572 76-77 15.06131656883814 28.31200350685869 32.504731051736854 24.121948872566314 78-79 15.06666239000973 27.862954528445115 32.21872961905679 24.851653462488375 80-81 15.232382846329024 27.457741283638583 32.05728581967476 25.252590050357636 82-83 15.232464276359048 27.585653877612117 31.39029514436467 25.791586701664166 84-85 15.24011140869993 27.152395768180092 31.015348098728207 26.59214472439177 86-87 15.287526528779383 27.117892024526757 30.709768471247344 26.884812975446515 88-89 15.507692636665919 26.780960323315266 30.36961007580374 27.341736964215073 90-91 15.65219715599273 25.99807548380199 30.758045546883352 27.591681813321927 92-93 15.37030503255605 25.69682778971678 30.717088451957103 28.215778725770065 94-95 15.097133570687793 25.174006479135258 30.649196523077908 29.079663427099035 96-97 15.323261806246059 25.301771605136263 30.085212389475146 29.289754199142532 98-99 15.379927510664912 25.09114625097561 29.86870663202574 29.660219606333726 100-101 15.349990912104008 24.92328746618768 30.428414108691236 29.298307513017075 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1585.0 1 918.5 2 319.0 3 365.5 4 255.5 5 216.0 6 417.0 7 703.0 8 900.0 9 892.5 10 657.0 11 446.0 12 403.5 13 388.5 14 400.0 15 431.5 16 456.0 17 517.5 18 563.5 19 614.0 20 667.0 21 742.0 22 835.5 23 886.0 24 977.0 25 1143.0 26 1389.0 27 1739.5 28 1936.5 29 2058.0 30 2264.0 31 2366.0 32 2475.5 33 2564.5 34 2705.0 35 2817.5 36 2892.5 37 2914.0 38 2644.0 39 2384.0 40 2253.0 41 2218.0 42 2246.5 43 2314.0 44 2436.5 45 2457.5 46 2382.5 47 2374.0 48 2469.0 49 2451.0 50 2603.0 51 2778.0 52 2492.0 53 1837.0 54 1204.5 55 959.0 56 845.5 57 712.5 58 667.5 59 649.5 60 633.0 61 638.0 62 607.0 63 544.5 64 481.5 65 458.5 66 439.0 67 392.5 68 332.0 69 295.0 70 272.5 71 232.5 72 187.5 73 155.5 74 123.5 75 95.5 76 71.5 77 47.5 78 37.5 79 29.5 80 17.5 81 14.0 82 10.5 83 3.0 84 1.0 85 1.0 86 1.5 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.009622478108862303 3 0.006414985405908201 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0010691642343180336 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.008018731757385251 26-27 0.027263687975109856 28-29 0.0010691642343180336 30-31 0.0 32-33 0.0032074927029541005 34-35 0.02352161315499674 36-37 0.009622478108862303 38-39 0.02619452374079182 40-41 0.020314120452042637 42-43 0.01496829928045247 44-45 0.009622478108862303 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 5.345821171590168E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 5.345821171590168E-4 84-85 0.0016037463514770502 86-87 5.345821171590168E-4 88-89 0.0 90-91 0.0010691642343180336 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 93531.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.87544236670195 #Duplication Level Percentage of deduplicated Percentage of total 1 83.70383073592146 41.74765585741626 2 8.392462860940213 8.371555954710203 3 2.799631288987974 4.188985470058055 4 1.3890973011211387 2.771273695352343 5 0.859611138502433 2.1436742898076573 6 0.4994748011747305 1.494691599576611 7 0.3901476987716779 1.3621152345211747 8 0.27653325901948594 1.1033774898162108 9 0.21007953010782654 0.9430028546685056 >10 1.3140688975112007 12.041996771124012 >50 0.10503976505391327 3.6437117105558587 >100 0.04072970481682351 4.2060920978071445 >500 0.006431006023708975 2.263420684051277 >1k 0.01286201204741795 13.718446290534688 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2865 3.0631555313211662 No Hit CTTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGT 2659 2.8429076990516515 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2210 2.3628529578428545 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1907 2.03889619484449 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1656 1.7705359720306637 No Hit ATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTT 1534 1.6400979354438634 RNA PCR Primer, Index 31 (95% over 22bp) ACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCT 838 0.8959596283585121 RNA PCR Primer, Index 31 (95% over 24bp) ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 667 0.7131325442901284 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 612 0.6543285114026366 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 390 0.41697405138403315 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 387 0.413766558681079 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 355 0.3795533031829019 No Hit CATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCT 341 0.36458500390244947 RNA PCR Primer, Index 31 (96% over 27bp) TTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTC 308 0.32930258416995434 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 270 0.28867434326586905 No Hit CACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTG 242 0.25873774470496413 RNA PCR Primer, Index 38 (95% over 24bp) TATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCT 198 0.21169451839497064 RNA PCR Primer, Index 31 (95% over 21bp) GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 174 0.18603457677133786 No Hit ATTTTAAACTTGCTAAAAAAACAACAAAATCAAAAAGTAAGTTTAGATTA 160 0.17106627749088538 No Hit AATCTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGCAAGTTTAAAATT 145 0.15502881397611487 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 139 0.14861382857020666 No Hit CTTTACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTC 133 0.14219884316429848 No Hit ACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGC 127 0.13578385775839028 RNA PCR Primer, Index 38 (96% over 25bp) GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 127 0.13578385775839028 No Hit AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 114 0.12188472271225582 No Hit TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 112 0.11974639424361976 No Hit GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAG 108 0.11546973730634763 No Hit GTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 104 0.11119308036907549 No Hit TTCCAGAAGAGCTGTCCCTCTTTTGGCTATAATCTAAACTTACTTTTTGA 99 0.10584725919748532 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 99 0.10584725919748532 No Hit CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 96 0.10263976649453121 No Hit CTATTAAAGGTTTTTTCCGTTCCAGAAGAGCTGTCCCTCTTTTGGCTATA 94 0.10050143802589516 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 94 0.10050143802589516 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0010691642343180336 0.0 10-11 0.0 0.0 0.0 0.0010691642343180336 0.0 12-13 0.0 0.0 0.0 0.0010691642343180336 0.0 14-15 0.0 0.0 0.0 0.0010691642343180336 0.0 16-17 0.0 0.0 0.0 0.0016037463514770502 0.0 18-19 0.0 0.0 0.0 0.002138328468636067 0.0 20-21 0.0 0.0 0.0 0.002672910585795084 0.0 22-23 0.0 0.0 0.0 0.009622478108862301 0.0 24-25 0.0 0.0 0.0 0.03154034491238199 0.0 26-27 0.0 0.0 0.0 0.07804898910521646 0.0 28-29 0.0 0.0 0.0 0.1940533085287231 0.0 30-31 0.0 0.0 0.0 0.4624135313425496 0.0 32-33 0.0 0.0 0.0 0.8622809549774941 0.0 34-35 0.0 0.0 0.0 1.3829639370903766 0.0 36-37 0.0 0.0 0.0 2.0549336583592606 0.0 38-39 0.0 0.0 0.0 2.89796965711903 0.0 40-41 0.0 0.0 0.0 3.7778918219627715 0.0 42-43 0.0 0.0 0.0 4.476590649089607 0.0 44-45 0.0 0.0 0.0 5.0849450984165685 0.0 46-47 0.0 0.0 0.0 5.738739027702045 0.0 48-49 0.0 0.0 0.0 6.406432092033658 0.0 50-51 0.0 0.0 0.0 7.074659738482428 0.0 52-53 0.0 0.0 0.0 7.600153959649742 0.0 54-55 0.0 0.0 0.0 8.106403224599331 0.0 56-57 0.0 0.0 0.0 8.65007323775005 0.0 58-59 0.0 0.0 0.0 9.169152473511456 0.0 60-61 0.0 0.0 0.0 9.7972864611733 0.0 62-63 0.0 0.0 0.0 10.37944638675947 0.0 64-65 0.0 0.0 0.0 10.872331098780084 0.0 66-67 0.0 0.0 0.0 11.448610621077504 0.0 68-69 0.0 0.0 0.0 12.050015502881397 0.0 70-71 0.0 0.0 0.0 12.717708567213009 0.0 72-73 0.0 0.0 0.0 13.364018346858261 0.0 74-75 0.0 0.0 0.0 13.980926110059766 0.0 76-77 0.0 0.0 0.0 14.551859811185597 0.0 78-79 0.0 0.0 0.0 15.184270455784713 0.0 80-81 0.0 0.0 0.0010691642343180336 15.821492339438262 0.0 82-83 0.0 0.0 0.0010691642343180336 16.482235836246808 0.0 84-85 0.0 0.0 0.0010691642343180336 17.124268958954787 0.0 86-87 0.0 0.0 0.0010691642343180336 17.698944734900728 0.0 88-89 0.0 0.0 0.0010691642343180336 18.297142124001667 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTACAC 15 6.5987615E-4 95.0 3 GCTGCGG 25 0.005006898 57.000004 8 CGCAGAA 45 9.579704E-4 42.22222 9 CTCTAGA 45 9.579704E-4 42.22222 2 GGTATCA 1275 0.0 42.098038 1 TAGATGG 60 8.232417E-5 39.583336 5 TGGATAT 50 0.0016082332 38.000004 9 TTCATCT 25 0.0016123572 38.000004 82-83 ATGGATA 55 0.0025675741 34.545456 8 CCTCTAG 55 0.0025675741 34.545456 1 GTATCAA 2005 0.0 34.11471 1 GAACTGT 30 0.0039425706 31.666668 58-59 CTGCCGG 30 0.0039425706 31.666668 54-55 CTAGATG 80 4.462485E-4 29.687498 4 TCTAGAT 65 0.0058170124 29.23077 3 GATGGAT 65 0.0058170124 29.23077 7 TATCAAC 2350 0.0 28.904255 2 AACGCAG 2420 0.0 28.06818 6 TCAACGC 2445 0.0 27.781185 4 ATCAACG 2450 0.0 27.724491 3 >>END_MODULE