FastQCFastQC Report
Thu 26 May 2016
SRR937940_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937940_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences95275
Sequences flagged as poor quality0
Sequence length101
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28562.9976384151141433No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21102.2146418262923118No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20122.1117816845972186No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15601.6373655208606663No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6420.6738388874311205No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6300.6612437680398845No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4150.4355812122802414No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3790.3977958541065337No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3720.3904487011283128No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2680.2812909997376017No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2020.21201784308580424No Hit
ATTTTAAACTTGCTAAAAAAACAACAAAATCAAAAAGTAAGTTTAGATTA1900.19942272369456834No Hit
AATCTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGCAAGTTTAAAATT1520.15953817895565467No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG1380.1448438729992128No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA1250.13119916032537393No Hit
TTCCAGAAGAGCTGTCCCTCTTTTGGCTATAATCTAAACTTACTTTTTGA1180.12385200734715297No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT1150.12070322749934399No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG1120.11755444765153503No Hit
GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAG970.10181054841249017No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC960.10076095512988716No Hit
GTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA960.10076095512988716No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCAATG250.004988977757.0523951
GTACCCA352.7623563E-454.3356131
CACACAG308.245559E-539.59888594-95
GAGCTAT500.001601124438.034931
TACCCAT500.001605270738.0149352
CTATAGA500.001605270738.0149354
GAGTAAA801.0605547E-535.6577451
GCTATAG550.00256285234.559033
GGTATCA13500.032.4001241
GTATCAA20250.032.165341
CCGCAAG300.00392504231.69577218-19
GAACTAG600.00392535731.6791089
TATAGAA804.4527958E-429.6991655
TGGGAAT404.4694127E-429.69916512-13
AGAACTA650.00580635129.2422568
AGTACTC2800.028.8506167
CGAAACT350.0083582527.15352228-29
AGCTATA700.00833760527.1535222
ACCCATG700.00833760527.1535223
TATCAAC24350.026.7353462
Produced by FastQC (version 0.11.5)