##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937940_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 95275 Sequences flagged as poor quality 0 Sequence length 101 %GC 37 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.26329047494096 33.0 31.0 34.0 31.0 34.0 2 32.460572028339016 34.0 31.0 34.0 31.0 34.0 3 32.445877722382576 34.0 31.0 34.0 30.0 34.0 4 36.01383363946471 37.0 35.0 37.0 35.0 37.0 5 36.01571241144057 37.0 35.0 37.0 35.0 37.0 6 36.104035686171606 37.0 35.0 37.0 35.0 37.0 7 36.031361847284174 37.0 35.0 37.0 35.0 37.0 8 36.0537076882708 37.0 35.0 37.0 35.0 37.0 9 37.61506166360535 39.0 37.0 39.0 35.0 39.0 10-11 37.65993177643663 39.0 37.0 39.0 35.0 39.0 12-13 37.56993440041984 39.0 37.0 39.0 35.0 39.0 14-15 38.61422723694568 40.0 38.0 41.0 34.0 41.0 16-17 38.53256363159276 40.0 38.0 41.0 34.0 41.0 18-19 38.83010758331147 40.0 38.0 41.0 35.0 41.0 20-21 38.89612175282078 40.0 38.0 41.0 35.0 41.0 22-23 38.72138021516662 40.0 38.0 41.0 35.0 41.0 24-25 38.53542902125426 40.0 38.0 41.0 34.5 41.0 26-27 38.22903699816321 40.0 37.0 41.0 34.0 41.0 28-29 37.9416950931514 40.0 36.5 41.0 33.5 41.0 30-31 37.31234846496983 39.0 35.0 41.0 33.0 41.0 32-33 36.64858042508528 38.0 35.0 41.0 33.0 41.0 34-35 36.145741275255844 38.0 35.0 41.0 31.5 41.0 36-37 35.74062450800315 38.0 35.0 41.0 31.0 41.0 38-39 35.23579113093676 38.0 35.0 40.0 29.5 41.0 40-41 34.808396746260826 38.0 35.0 40.0 27.0 41.0 42-43 34.14028863815272 37.5 35.0 40.0 22.5 41.0 44-45 33.28184728417738 37.0 34.0 40.0 7.5 41.0 46-47 32.16453424298084 36.5 33.0 40.0 2.0 41.0 48-49 31.169860928890056 36.0 32.5 40.0 2.0 41.0 50-51 30.27712936237208 35.0 30.5 40.0 2.0 41.0 52-53 29.52724219364996 35.0 28.5 40.0 2.0 41.0 54-55 28.971151928627656 35.0 26.5 39.5 2.0 41.0 56-57 28.26275518236683 35.0 22.5 39.0 2.0 41.0 58-59 27.49115192862766 35.0 13.0 39.0 2.0 41.0 60-61 26.638803463657833 35.0 2.0 38.5 2.0 40.5 62-63 25.84694830753083 34.0 2.0 38.0 2.0 40.0 64-65 25.04628181579638 34.0 2.0 38.0 2.0 40.0 66-67 24.118493833639462 33.0 2.0 37.0 2.0 40.0 68-69 23.299674626082393 33.0 2.0 36.0 2.0 39.0 70-71 22.752049330884283 32.5 2.0 36.0 2.0 39.0 72-73 22.29639464707426 32.0 2.0 35.0 2.0 38.5 74-75 21.86621359223301 31.0 2.0 35.0 2.0 37.5 76-77 20.803474153765414 29.5 2.0 34.0 2.0 36.0 78-79 21.26818157963789 31.0 2.0 35.0 2.0 36.0 80-81 21.042099186565203 31.0 2.0 35.0 2.0 36.0 82-83 20.67329834689058 30.0 2.0 35.0 2.0 35.5 84-85 20.321632117554447 29.5 2.0 35.0 2.0 35.0 86-87 19.943269483075305 29.0 2.0 34.5 2.0 35.0 88-89 19.470086591445813 28.0 2.0 34.0 2.0 35.0 90-91 19.146827604303333 27.0 2.0 34.0 2.0 35.0 92-93 18.670973497769616 25.5 2.0 34.0 2.0 35.0 94-95 18.1942744686434 24.5 2.0 34.0 2.0 35.0 96-97 17.603180267646287 21.5 2.0 34.0 2.0 35.0 98-99 16.78002099186565 8.5 2.0 33.5 2.0 35.0 100-101 13.97399632642351 2.0 2.0 29.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.0650527310359621 1101 2 -0.043738990592629534 1101 3 -0.05081891422983631 1101 4 -0.11570741312710453 1101 5 0.06778183154739281 1101 6 -0.038382004541254844 1101 7 0.1156592780588852 1101 8 0.2548713437650605 1101 9 -0.529476040384985 1101 10-11 0.21568168763094064 1101 12-13 0.1556696022136279 1101 14-15 0.2106854670099736 1101 16-17 0.659838324162692 1101 18-19 0.008971817637736024 1101 20-21 -0.05280030018832349 1101 22-23 -0.22553192803952982 1101 24-25 0.14075872787684318 1101 26-27 -0.20549225277645888 1101 28-29 -0.11794115522027937 1101 30-31 0.13171657457787944 1101 32-33 0.5863791347965517 1101 34-35 -0.6649168545586832 1101 36-37 -0.7561851530196648 1101 38-39 -0.40740303629142716 1101 40-41 -1.6200206064096179 1101 42-43 -2.17330543827293 1101 44-45 -1.1908643369465963 1101 46-47 -1.8418025918715308 1101 48-49 -1.2204484095203867 1101 50-51 -1.5742550175842709 1101 52-53 -1.5065428840678408 1101 54-55 -1.2765022174870602 1101 56-57 -1.3664842161877822 1101 58-59 -1.6594145286149526 1101 60-61 -1.5056818757692412 1101 62-63 -1.349895753267507 1101 64-65 -1.3077484985474648 1101 66-67 -0.8863340950184124 1101 68-69 -0.052157350990007956 1101 70-71 -0.37401404004185324 1101 72-73 -1.0419018302374816 1101 74-75 -1.1164334238947546 1101 76-77 -1.2456096485514507 1101 78-79 -1.3314538747114604 1101 80-81 -1.7910540668447545 1101 82-83 -1.6608560553041016 1101 84-85 -1.9383665705262842 1101 86-87 -1.4758099420669843 1101 88-89 -1.8821505654954187 1101 90-91 -1.8811938304965352 1101 92-93 -1.5444780215896152 1101 94-95 -1.8855616001228732 1101 96-97 -1.8449132251175655 1101 98-99 -0.8971972947843767 1101 100-101 -0.6534218566744183 1102 1 0.4145885960148661 1102 2 0.4536449756520966 1102 3 0.3839490182596137 1102 4 0.48327992864504665 1102 5 0.5201025066528757 1102 6 0.31199774229418864 1102 7 0.3980221472572012 1102 8 0.3772342129633728 1102 9 0.5537307106698677 1102 10-11 0.3166943458587994 1102 12-13 0.35870757689718147 1102 14-15 0.567563104140774 1102 16-17 0.3968847376647986 1102 18-19 0.7332756151060948 1102 20-21 0.5102376744952224 1102 22-23 0.7108393799773509 1102 24-25 0.7718979683831719 1102 26-27 0.5016807430041368 1102 28-29 0.22028669288098257 1102 30-31 -0.11874756044321799 1102 32-33 0.23177997867841071 1102 34-35 0.37837428468181855 1102 36-37 1.0166418511997435 1102 38-39 0.6060146852275636 1102 40-41 0.26124521637519393 1102 42-43 -0.05203961548811975 1102 44-45 0.1571525406905394 1102 46-47 0.5349906570736209 1102 48-49 -0.47698849390857134 1102 50-51 -1.0851410935336396 1102 52-53 -1.409327694194424 1102 54-55 -1.705700529723348 1102 56-57 -1.513657211968372 1102 58-59 -1.7681908999229705 1102 60-61 -1.8095637323093214 1102 62-63 -1.415212208963709 1102 64-65 -2.056440481669828 1102 66-67 -1.8232117321492147 1102 68-69 -2.0179801358001335 1102 70-71 -1.7906385126156898 1102 72-73 -2.4524503534442346 1102 74-75 -3.4936486137681726 1102 76-77 -2.883499943910099 1102 78-79 -2.6105256046692666 1102 80-81 -2.005737611148554 1102 82-83 -2.5958771523505177 1102 84-85 -2.591193574745688 1102 86-87 -2.6429829378475773 1102 88-89 -1.5424037300523814 1102 90-91 -1.3117423537032877 1102 92-93 -0.6990771777077605 1102 94-95 -1.199148098013172 1102 96-97 -1.790060904442461 1102 98-99 -1.5306994044890168 1102 100-101 -1.290215105619568 1103 1 -0.24673021470047019 1103 2 -0.016917285166272222 1103 3 -0.18508248012130935 1103 4 -0.07059113405733797 1103 5 -0.05702437000299909 1103 6 -0.17652153942497506 1103 7 -0.1390694041116589 1103 8 -0.5900898965450168 1103 9 -0.12451480007491256 1103 10-11 -0.28059738213649865 1103 12-13 -0.2645629559259035 1103 14-15 -0.36016724616832363 1103 16-17 -0.10326245103110665 1103 18-19 -0.4680049265483035 1103 20-21 -0.07814913739762375 1103 22-23 0.279429312270544 1103 24-25 -0.5017994116580411 1103 26-27 -0.15161628378421454 1103 28-29 -0.2809644110342333 1103 30-31 -0.09983381301901773 1103 32-33 -0.16695419853677862 1103 34-35 -0.033754063861010764 1103 36-37 0.745520273336929 1103 38-39 -0.2441472223379435 1103 40-41 0.4216073005671248 1103 42-43 -0.41493334524967196 1103 44-45 -0.25939146872953955 1103 46-47 0.1609105864230429 1103 48-49 0.4861407377664335 1103 50-51 0.5101635870668915 1103 52-53 0.25066641825774383 1103 54-55 0.4445055344509221 1103 56-57 0.9419653962153198 1103 58-59 1.4102753938656676 1103 60-61 1.0188142482617693 1103 62-63 0.7903368048720303 1103 64-65 1.527677858041681 1103 66-67 1.488239548392439 1103 68-69 0.6490054397076683 1103 70-71 0.772652626624815 1103 72-73 0.48057878991755487 1103 74-75 0.37542704122152415 1103 76-77 1.0601267855570775 1103 78-79 0.514437598156757 1103 80-81 0.5619691889691971 1103 82-83 0.9945703012850444 1103 84-85 1.0752768403264312 1103 86-87 0.5277559494058863 1103 88-89 0.8685471089231847 1103 90-91 -0.03568995452754464 1103 92-93 0.21032817996077213 1103 94-95 1.019942275846116 1103 96-97 0.9070247593785581 1103 98-99 0.6114848757582578 1103 100-101 0.6303765929379832 1104 1 -0.0344229243947467 1104 2 0.0913920955496863 1104 3 -0.11583764082159576 1104 4 0.0654161823785131 1104 5 -0.11124438568107564 1104 6 -0.09591009442889487 1104 7 -0.37228079684748394 1104 8 -0.41554064125367063 1104 9 -0.04063708907412433 1104 10-11 -0.0013969640544431172 1104 12-13 -0.045004555089739995 1104 14-15 -0.5036216490948959 1104 16-17 -0.4267534361369343 1104 18-19 0.12155608730066803 1104 20-21 -0.10695760355911688 1104 22-23 -0.6142210666163095 1104 24-25 -0.024859249651242976 1104 26-27 0.204994638609314 1104 28-29 0.08453075489207862 1104 30-31 0.5333645146527886 1104 32-33 -0.10515644572779337 1104 34-35 -0.8703101129856563 1104 36-37 -1.130492269124531 1104 38-39 -1.2898749464037849 1104 40-41 -1.2782228536006315 1104 42-43 -1.4960020674864083 1104 44-45 -0.9411265092312391 1104 46-47 -0.8425516555419463 1104 48-49 -1.4444184469735681 1104 50-51 -1.1558280512921364 1104 52-53 -1.4573294009217719 1104 54-55 -1.722270007749234 1104 56-57 -3.110229534539844 1104 58-59 -4.073197300150532 1104 60-61 -4.30650584580669 1104 62-63 -5.182030584728178 1104 64-65 -5.189920783191663 1104 66-67 -4.392476417115791 1104 68-69 -4.651033755484388 1104 70-71 -4.092515912701028 1104 72-73 -4.247145275930368 1104 74-75 -4.333287356479023 1104 76-77 -3.724560959412873 1104 78-79 -4.729356496434306 1104 80-81 -5.514200134260488 1104 82-83 -4.67808451972358 1104 84-85 -4.511475184758492 1104 86-87 -4.823824923340393 1104 88-89 -4.489566295832496 1104 90-91 -5.2244148042793075 1104 92-93 -4.288822590878006 1104 94-95 -4.146310663793287 1104 96-97 -5.185737195155021 1104 98-99 -4.1771223884173345 1104 100-101 -3.446380658172547 1105 1 0.6314295426301655 1105 2 0.5078552123059197 1105 3 0.49236949156726695 1105 4 0.4425534564381124 1105 5 0.21850646922855077 1105 6 0.4142266911109189 1105 7 0.24667377081250663 1105 8 0.43240757564911547 1105 9 0.2986399016439947 1105 10-11 0.53676864415268 1105 12-13 0.4863217761266796 1105 14-15 0.49271445251721957 1105 16-17 0.6621571647424034 1105 18-19 0.6563631219855708 1105 20-21 0.5182866563334088 1105 22-23 0.5069318400764118 1105 24-25 0.12749785831162797 1105 26-27 0.516609196498905 1105 28-29 0.43705884477972035 1105 30-31 0.7665716470416513 1105 32-33 1.2515240623327841 1105 34-35 1.5650831454413137 1105 36-37 1.186496006400624 1105 38-39 1.4499882680564014 1105 40-41 1.7469359153295088 1105 42-43 1.634738039987944 1105 44-45 1.5139907355171758 1105 46-47 1.7737771182734008 1105 48-49 1.8596240542477283 1105 50-51 2.4009623737200734 1105 52-53 2.663457115932161 1105 54-55 1.5837876375854059 1105 56-57 1.9931534649716376 1105 58-59 1.9205130076169326 1105 60-61 1.764390587998875 1105 62-63 2.0666259858629275 1105 64-65 2.7534109217423897 1105 66-67 2.9798977890395584 1105 68-69 2.084799170749122 1105 70-71 1.8596091483639476 1105 72-73 1.833460488603098 1105 74-75 1.4553057065400274 1105 76-77 2.2267091920282596 1105 78-79 1.7764446760131776 1105 80-81 1.6645981070682865 1105 82-83 1.4974772780292298 1105 84-85 1.6356914004882128 1105 86-87 1.7225233912663498 1105 88-89 1.0071972605915391 1105 90-91 1.186125010083174 1105 92-93 0.7989277755118316 1105 94-95 0.12219202306553356 1105 96-97 0.4666809777809853 1105 98-99 1.6835273428967845 1105 100-101 1.454694085354566 1106 1 0.29669931738134636 1106 2 0.15610036683708728 1106 3 0.17440196930429863 1106 4 -0.1079965697536096 1106 5 -0.55310170258916 1106 6 0.07438907979609866 1106 7 0.11100064352073247 1106 8 -0.18689572450803382 1106 9 0.11084524475557345 1106 10-11 -0.3329930111642341 1106 12-13 0.4036214094425432 1106 14-15 0.10088627022282282 1106 16-17 -0.06963958748389842 1106 18-19 0.06246579354135662 1106 20-21 0.24189849499239102 1106 22-23 0.14731947758296116 1106 24-25 0.2879876435394948 1106 26-27 -0.08822317647123867 1106 28-29 0.2066371580327342 1106 30-31 0.2693872573088001 1106 32-33 0.3571421870053868 1106 34-35 -0.37768793889603813 1106 36-37 -0.5440566389634398 1106 38-39 0.09404274684110447 1106 40-41 0.3828709598554454 1106 42-43 -0.1472813418873855 1106 44-45 -0.21512136505904067 1106 46-47 -0.6426058047229368 1106 48-49 -0.8232596545003048 1106 50-51 -0.8299176681866776 1106 52-53 -0.5383501129835011 1106 54-55 -0.8826869564428854 1106 56-57 -1.0864038949026416 1106 58-59 -0.709856295683224 1106 60-61 -0.31307143400217186 1106 62-63 0.278778945527673 1106 64-65 -0.9920055266599164 1106 66-67 -1.7420770669059635 1106 68-69 -2.1798681943635003 1106 70-71 -1.7996365300016883 1106 72-73 -1.7692110671852745 1106 74-75 -1.6477587250995747 1106 76-77 -1.5683405722462318 1106 78-79 -1.8205301799323443 1106 80-81 -2.109367319856805 1106 82-83 -1.952823926790046 1106 84-85 -1.422061349642746 1106 86-87 -1.3964525123481053 1106 88-89 -1.7956445413990316 1106 90-91 -1.3875392120226415 1106 92-93 -1.862871595886805 1106 94-95 -2.422268427432112 1106 96-97 -2.3790497713023058 1106 98-99 -2.9525386602462245 1106 100-101 -3.2933415353892794 1107 1 -0.17852115212554764 1107 2 0.03474898191596054 1107 3 0.10285806171518175 1107 4 -0.20065586673535307 1107 5 -0.07104978357288871 1107 6 -0.5513716952629011 1107 7 -0.2432410655837245 1107 8 -0.3155753916301336 1107 9 -0.05882311942279017 1107 10-11 0.09856828556909392 1107 12-13 0.17340156097652226 1107 14-15 0.10051364570413313 1107 16-17 -0.059268205046926425 1107 18-19 -0.0636055019498869 1107 20-21 0.38163268950239626 1107 22-23 0.4791416114793705 1107 24-25 0.17663501653663616 1107 26-27 -0.3934647613675182 1107 28-29 -0.7420648665604901 1107 30-31 -0.21185730858363172 1107 32-33 -0.43499543565017973 1107 34-35 -0.3962570607442544 1107 36-37 -0.4645013042224164 1107 38-39 -0.37049581979658797 1107 40-41 -0.6905360703271413 1107 42-43 0.09411724213943984 1107 44-45 -0.9103145087679039 1107 46-47 -0.7699812860296049 1107 48-49 -1.0551563133004542 1107 50-51 -1.0328117186151964 1107 52-53 -1.2618006160266013 1107 54-55 -1.5237874408547576 1107 56-57 -1.551501398318365 1107 58-59 -2.0461499238039558 1107 60-61 -1.9003897795493074 1107 62-63 -0.843504000690185 1107 64-65 -1.2799821755234113 1107 66-67 -1.4747189747434994 1107 68-69 -0.9325697221240254 1107 70-71 -0.8922958269834318 1107 72-73 -0.5587403182099919 1107 74-75 -1.0690107434823837 1107 76-77 -1.3942007138435493 1107 78-79 -1.434615386738951 1107 80-81 -0.9073427266385714 1107 82-83 -1.2821618972285052 1107 84-85 -1.976029800766831 1107 86-87 -2.0828889798676684 1107 88-89 -2.025243349000572 1107 90-91 -1.7427745865102828 1107 92-93 -2.0823474373971784 1107 94-95 -1.8108536963428072 1107 96-97 -1.640445871165678 1107 98-99 -1.417884580007744 1107 100-101 -0.40276893571221883 1108 1 -0.06379215345248213 1108 2 0.12365374868129209 1108 3 0.30812498015960443 1108 4 0.2019163492491316 1108 5 -0.08799374601036902 1108 6 0.2578556192211252 1108 7 0.17994970710179103 1108 8 -0.1180659499643113 1108 9 0.3242533325523098 1108 10-11 0.31587970743292715 1108 12-13 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-2.5401018300079325 2316 96-97 -2.60640747799113 2316 98-99 -1.8226570648993192 2316 100-101 -1.29474369575488 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 2.0 4 0.0 5 5.0 6 21.0 7 36.0 8 97.0 9 187.0 10 342.0 11 624.0 12 925.0 13 1419.0 14 1684.0 15 2249.0 16 2934.0 17 3909.0 18 3757.0 19 2637.0 20 2340.0 21 2435.0 22 2385.0 23 2343.0 24 2654.0 25 2457.0 26 1801.0 27 1315.0 28 1308.0 29 1598.0 30 1903.0 31 2216.0 32 2796.0 33 3521.0 34 5095.0 35 8269.0 36 8982.0 37 12924.0 38 7688.0 39 416.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.122432270728154 23.31398461199341 14.771856532555185 17.791726584723257 2 15.427413682696647 23.75418595618262 40.75625400224651 20.062146358874227 3 18.60902058338844 32.33932676260352 33.7353444384965 15.31630821551154 4 10.500131199160325 23.756494358436107 33.46733140907898 32.276043033324584 5 8.385288746142704 42.64752188424964 33.722736528328824 15.244452841278838 6 22.41826292311729 40.62030963001837 16.339018630280766 20.622408816583572 7 18.418262923117293 42.366832852269745 18.6659669378116 20.548937286801365 8 25.23956966675413 33.91655733403306 20.225662555759644 20.61821044345316 9 24.967725006559956 16.81448438729992 20.469168197323537 37.74862240881659 10-11 22.517974284964577 29.90186302807662 25.219627394384673 22.360535292574127 12-13 25.996462852074792 29.42130370665813 26.406331179906477 18.175902261360594 14-15 22.39884544738914 28.871687221201785 28.176856468118604 20.552610863290475 16-17 16.58129795429975 34.29880448815509 28.169051042792816 20.950846514752346 18-19 16.093413802151666 29.383888743112045 35.81999475203359 18.7027027027027 20-21 17.166098136971925 27.95801626869588 36.30018367882445 18.575701915507743 22-23 16.387299921280505 30.907373392810282 35.85568092364208 16.84964576226712 24-25 16.635090242097583 31.615435885863036 36.482053919587706 15.267419952451677 26-27 16.41602234015191 31.3528494716785 36.943661454314494 15.287466733855092 28-29 15.73508418356636 30.622559516311874 37.67896880379561 15.963387496326153 30-31 14.648585103703082 29.661390544965993 38.970526492568645 16.71949785876228 32-33 13.187614799265285 31.948045132511147 38.36578325898714 16.49855680923642 34-35 14.546790531674802 30.93056211620217 38.125754474360996 16.39689287776203 36-37 13.813769686537322 31.73829303748642 38.45257650263187 15.99536077334439 38-39 14.241854899655722 30.317407003106894 38.14184650264506 17.298891594592327 40-41 14.286389041958591 30.357658295940592 38.71054081712981 16.645411844971004 42-43 15.795384768792346 29.33248663031442 38.85317224983337 16.018956351059867 44-45 14.243505641563894 30.798740488060876 37.94699553922855 17.01075833114668 46-47 16.235633691944372 29.07635791130937 35.882445552348464 18.805562844397798 48-49 15.69771713461034 28.64340068223563 35.95801626869588 19.70086591445815 50-51 14.455535961708025 28.840219171180248 36.87649578032498 19.82774908678675 52-53 15.279600256138398 29.292680110433437 37.29648019651274 18.131239436915422 54-55 15.160262634426944 28.996331305666796 35.055712718665205 20.78769334124106 56-57 16.768503937007875 30.30656167979003 32.68661417322834 20.238320209973754 58-59 14.819695195896745 31.70414157693863 34.32642283038381 19.149740396780814 60-61 16.151177498674755 33.47644215377025 30.809159664306595 19.563220683248396 62-63 14.612097476979935 34.45187572839999 30.654746280566552 20.281280514053527 64-65 14.300263536427874 33.92847768339931 30.793864115998026 20.977394664174795 66-67 15.010445426582825 33.446885793170054 30.17993428304482 21.362734497202304 68-69 15.25053551178126 31.96690327187198 30.290541391910626 22.492019824436138 70-71 15.192537846179688 30.91418733071577 31.085308753438174 22.807966069666367 72-73 15.658097767327616 29.194264540711895 31.314621012522696 23.83301667943779 74-75 15.239255044407585 28.85705587166943 30.917336804753603 24.98635227916938 76-77 14.83705694607761 28.35755081471527 32.32141357298841 24.483978666218714 78-79 15.247875093842367 27.777340284230807 31.949453745557253 25.025330876369573 80-81 15.206071930966427 27.471708412941698 31.727518948539757 25.59470070755212 82-83 14.942770135461513 27.7165809093773 31.03696314186706 26.30368581329413 84-85 15.28382216295954 27.019932909511834 30.65426350077957 27.04198142674905 86-87 15.347379363570976 27.003869396069703 30.319369556200748 27.329381684158577 88-89 15.493002110391316 26.64920256606785 29.623699379481955 28.23409594405888 90-91 15.628740628740628 25.903000903000905 30.143955143955143 28.324303324303322 92-93 15.401455255614705 25.631818229544002 30.133030942556253 28.833695572285045 94-95 15.293358040115276 25.386218445047533 29.970446349362994 29.349977165474193 96-97 15.465898174831894 25.39697537571587 29.569613076959417 29.567513372492822 98-99 15.671978668010414 25.225707566977405 29.181888804904677 29.9204249601075 100-101 15.876965068367321 24.880980500223078 29.40083457995433 29.841219851455264 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1533.0 1 872.0 2 299.0 3 369.5 4 249.5 5 202.0 6 397.0 7 671.5 8 847.5 9 809.0 10 582.0 11 396.0 12 391.5 13 413.5 14 396.5 15 415.5 16 448.0 17 478.5 18 543.5 19 624.0 20 696.0 21 728.0 22 789.0 23 892.5 24 984.5 25 1152.0 26 1436.5 27 1805.0 28 1935.5 29 2025.0 30 2270.0 31 2383.0 32 2531.0 33 2660.5 34 2773.5 35 2928.0 36 3016.0 37 3007.5 38 2792.5 39 2457.5 40 2284.5 41 2284.0 42 2312.5 43 2361.0 44 2447.0 45 2527.5 46 2496.5 47 2459.5 48 2528.5 49 2604.5 50 2748.5 51 2872.5 52 2583.5 53 1887.5 54 1290.5 55 1012.0 56 857.0 57 745.5 58 703.5 59 673.5 60 668.5 61 661.0 62 597.0 63 526.5 64 481.0 65 453.0 66 403.0 67 365.0 68 340.5 69 317.0 70 297.5 71 234.5 72 181.5 73 165.0 74 130.0 75 107.5 76 82.0 77 50.0 78 33.5 79 27.0 80 18.5 81 9.0 82 5.0 83 2.0 84 0.5 85 0.5 86 0.5 87 1.5 88 1.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.006297559695617947 2 0.01679349252164786 3 0.004198373130411965 4 0.0 5 0.0010495932826029913 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 5.247966413014956E-4 14-15 0.0 16-17 0.0020991865652059826 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0036735764891104696 26-27 0.021516662293361324 28-29 0.007347152978220939 30-31 0.0031487798478089736 32-33 0.0 34-35 0.010495932826029914 36-37 5.247966413014956E-4 38-39 0.0031487798478089736 40-41 0.0026239832065074785 42-43 0.0036735764891104696 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.007347152978220939 52-53 0.014694305956441878 54-55 0.009971136184728417 56-57 0.026239832065074783 58-59 0.03516137496720021 60-61 0.009971136184728417 62-63 0.03358698504329572 64-65 0.03358698504329572 66-67 0.018892679086853846 68-69 0.04093413802151666 70-71 0.02204145893466282 72-73 0.00839674626082393 74-75 0.02204145893466282 76-77 0.028339018630280766 78-79 0.03726056153240619 80-81 0.01784308580425085 82-83 0.04723169771713461 84-85 0.03201259511939124 86-87 0.04250852794542115 88-89 0.03358698504329572 90-91 0.03883495145631068 92-93 0.0356861716085017 94-95 0.025715035423773288 96-97 0.024665442141170297 98-99 0.019942272369456834 100-101 0.01836788244555235 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 95275.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.86407766990291 #Duplication Level Percentage of deduplicated Percentage of total 1 83.93323229771828 41.85253214379428 2 8.084954112991497 8.062975596956178 3 2.8163677696387976 4.213067436368408 4 1.391344615643681 2.7751246392023092 5 0.825124189610171 2.0572028339018633 6 0.5472762482108277 1.6373655208606663 7 0.38519828239454407 1.344528995014432 8 0.22943504251915467 0.9152453424298084 9 0.2610086722236255 1.1713461033849384 >10 1.368190620527069 12.574127525583837 >50 0.0968257977603772 3.41747572815534 >100 0.04209817293929443 4.236158488585673 >500 0.006314725940894166 2.2597743374442403 >1k 0.012629451881788331 13.483075308318027 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGT 2804 2.9430595644187876 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2759 2.8958278667016533 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2114 2.2188401994227234 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1914 2.0089215429021254 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1679 1.7622671214904226 No Hit ATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTT 1576 1.6541590133823143 RNA PCR Primer, Index 31 (95% over 22bp) ACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCT 899 0.9435843610600892 RNA PCR Primer, Index 31 (95% over 24bp) ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 670 0.7032274993440042 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 584 0.612962477040147 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 420 0.44082917869325633 No Hit CATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCT 384 0.4030438205195486 RNA PCR Primer, Index 31 (96% over 27bp) GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 369 0.38729992128050383 No Hit TTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTC 323 0.33901863028076623 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 314 0.3295722907373393 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 261 0.2739438467593807 No Hit CACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTG 248 0.26029913408554184 RNA PCR Primer, Index 38 (95% over 24bp) GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 198 0.20781946995539227 No Hit TATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCT 181 0.18997638415114143 RNA PCR Primer, Index 31 (95% over 21bp) ATTTTAAACTTGCTAAAAAAACAACAAAATCAAAAAGTAAGTTTAGATTA 164 0.1721332983468906 No Hit AATCTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGCAAGTTTAAAATT 147 0.15429021254263972 No Hit CTTTACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTC 128 0.13434794017318288 No Hit TTCCAGAAGAGCTGTCCCTCTTTTGGCTATAATCTAAACTTACTTTTTGA 124 0.13014956704277092 No Hit AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 123 0.12909997376016794 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 123 0.12909997376016794 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 115 0.12070322749934399 No Hit ACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGC 105 0.11020729467331408 RNA PCR Primer, Index 38 (96% over 25bp) GTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 104 0.1091577013907111 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 104 0.1091577013907111 No Hit CTATTAAAGGTTTTTTCCGTTCCAGAAGAGCTGTCCCTCTTTTGGCTATA 101 0.10600892154290212 No Hit GGTGATAGCTGGTTACCCAAAAAATGAATTTAAGTTCAATTTTAAACTTG 100 0.10495932826029913 No Hit CTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTC 98 0.10286014169509315 No Hit GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAG 97 0.10181054841249017 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 97 0.10181054841249017 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 5.247966413014956E-4 0.0 18-19 0.0 0.0 0.0 0.001574389923904487 0.0 20-21 0.0 0.0 0.0 0.0036735764891104696 0.0 22-23 0.0 0.0 0.0 0.005772763054316453 0.0 24-25 0.0 0.0 0.0 0.022566255575964315 0.0 26-27 0.0 0.0 0.0 0.07137234321700342 0.0 28-29 0.0 0.0 0.0 0.19942272369456837 0.0 30-31 0.0 0.0 0.0 0.47179218053004457 0.0 32-33 0.0 0.0 0.0 0.8743112044082918 0.0 34-35 0.0 0.0 0.0 1.4389923904487012 0.0 36-37 0.0 0.0 0.0 2.1039097349776963 0.0 38-39 0.0 0.0 0.0 2.919968512201522 0.0 40-41 0.0 0.0 0.0 3.7869325636315927 0.0 42-43 0.0 0.0 0.0 4.463395434269221 0.0 44-45 0.0 0.0 0.0 5.094200997113619 0.0 46-47 0.0 0.0 0.0 5.754919968512201 0.0 48-49 0.0 0.0 0.0 6.4324324324324325 0.0 50-51 0.0 0.0 0.0 7.111519286276568 0.0 52-53 0.0 0.0 0.0 7.642613487273682 0.0 54-55 0.0 0.0 0.0 8.153765415901338 0.0 56-57 0.0 0.0 0.0 8.728417738126476 0.0 58-59 0.0 0.0 0.0 9.283127787982156 0.0 60-61 0.0 0.0 0.0 9.869325636315928 0.0 62-63 0.0 0.0 0.0 10.426659669378115 0.0 64-65 0.0 0.0 0.0 10.925741275255838 0.0 66-67 0.0 0.0 0.0 11.466806612437681 0.0 68-69 0.0 0.0 0.0 12.05247966413015 0.0 70-71 0.0 0.0 0.0 12.760955129887169 0.0 72-73 0.0 0.0 0.0 13.398583049068485 0.0 74-75 0.0 0.0 0.0 13.973235371293622 0.0 76-77 0.0 0.0 0.0 14.556284439779585 0.0 78-79 0.0 0.0 0.0 15.162424560482812 0.0 80-81 0.0 0.0 0.0 15.815796378903176 0.0 82-83 0.0 0.0 0.0 16.438729992128053 0.0 84-85 0.0 0.0 0.0 17.078457097874573 0.0 86-87 0.0 0.0 0.0 17.67829965888218 0.0 88-89 0.0 0.0 0.0 18.304382051954867 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCTAGGA 15 6.609445E-4 94.96273 2 TCCTAGG 15 6.609445E-4 94.96273 1 GAGAGAC 20 0.002071445 71.22205 9 TAGGAGA 20 0.002071445 71.22205 4 CAAAGCA 25 0.005014969 56.97764 4 GGGTAAT 40 5.373563E-4 47.481365 7 GTATTAA 55 4.940386E-5 43.16488 1 ATGGGGG 60 8.252639E-5 39.567806 7 GGTATCA 1315 0.0 38.273952 1 TTATCGA 25 0.0016113807 38.00504 56-57 TAAGATC 25 0.0016113807 38.00504 22-23 GAGTAAA 75 6.8742047E-6 37.985092 1 ATGGGGC 65 1.3221077E-4 36.524128 5 TAAATCA 55 0.0025727605 34.531902 7 CATGGGG 380 0.0 33.73676 4 GAGACAC 30 0.003930064 31.687511 76-77 ATGGGGT 180 5.456968E-12 31.654243 5 GGGCTTG 60 0.003940505 31.654243 9 ACATGAA 30 0.0039503346 31.654243 16-17 ATAAATC 60 0.003940505 31.654243 6 >>END_MODULE