FastQCFastQC Report
Thu 26 May 2016
SRR937939_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937939_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1942682
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99530.5123329500144645No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83360.4290975054074728No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52470.27009052433697334No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA24860.12796741823932067No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA24640.12683496321065413No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA21980.11314255240950397No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA21820.11231894875229192No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20040.10315635806580799No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA49900.049.2977751
GTATCAA82400.036.507351
ATCAACG104050.028.9550723
TATCAAC104150.028.9272752
CAACGCA105300.028.5662275
TCAACGC105550.028.4985644
AACGCAG107900.027.8288216
GTACATG82000.024.9414441
TACATGG80350.024.7211062
ACGCAGA121950.024.3071967
CGCAGAG122900.024.0428
ACATGGG83500.022.764133
GCAGAGT131300.021.9973819
GAGTACT79700.021.93136612-13
TAGGACG4450.021.3573574
GTGTAGC12950.021.3518921
TAGCCCT15900.021.2196754
GTACTTT84500.020.77281814-15
CAGAGTA127050.020.56485710-11
GAGTACA65800.019.9968381
Produced by FastQC (version 0.11.5)