FastQCFastQC Report
Thu 26 May 2016
SRR937939_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937939_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1942682
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94150.48463927704070975No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82820.42631784306438214No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53800.2769367297375484No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA25280.1301293778395023No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA25160.12951167509659328No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA21670.11154682032365563No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20990.10804650478050447No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA20790.10701700020898942No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA44700.050.76021
GTATCAA78050.040.650451
ATCAACG100250.031.4563263
TCAACGC100600.031.3444634
TATCAAC102200.030.9050622
CAACGCA102450.030.824885
AACGCAG105650.029.8946446
ACGCAGA121400.025.859737
CGCAGAG122250.025.446838
TACATGG80200.025.4445862
GTACATG81450.025.2876971
ACATGGG81950.023.9126193
TGGTATC22400.023.1467972
GCAGAGT133500.022.8755199
GAGTACT80050.022.2193712-13
GTGGTAT26350.020.3990651
GTACTTT85500.020.35865614-15
AGAGTAC120600.020.22247710-11
CAGAGTA128900.020.06255310-11
AGTACTT84100.019.6810412-13
Produced by FastQC (version 0.11.5)