##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937939_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1942682 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.4554765010434 33.0 31.0 34.0 31.0 34.0 2 32.678778101614164 34.0 31.0 34.0 31.0 34.0 3 32.74547712904119 34.0 31.0 34.0 31.0 34.0 4 36.20988252323335 37.0 35.0 37.0 35.0 37.0 5 36.135732456469974 37.0 35.0 37.0 35.0 37.0 6 36.194217066920885 37.0 37.0 37.0 35.0 37.0 7 36.165861422507646 37.0 37.0 37.0 35.0 37.0 8 36.161371238318985 37.0 37.0 37.0 35.0 37.0 9 37.84739138984147 39.0 38.0 39.0 35.0 39.0 10-11 37.814193213299966 39.0 38.0 39.0 35.0 39.0 12-13 37.735677532401084 39.0 38.0 39.0 35.0 39.0 14-15 38.964431131806435 40.0 38.0 41.0 35.5 41.0 16-17 38.87200478513725 40.0 38.0 41.0 35.0 41.0 18-19 39.13188648476694 40.0 38.5 41.0 36.0 41.0 20-21 39.107313240149445 40.0 39.0 41.0 36.0 41.0 22-23 38.99794742526054 40.0 38.5 41.0 35.0 41.0 24-25 38.88844597314434 40.0 38.0 41.0 35.0 41.0 26-27 38.71845778156178 40.0 38.0 41.0 35.0 41.0 28-29 38.593311720600695 40.0 38.0 41.0 34.5 41.0 30-31 38.349360832086774 40.0 38.0 41.0 34.0 41.0 32-33 38.11078318530774 40.0 37.5 41.0 34.0 41.0 34-35 38.009938837133404 40.0 37.5 41.0 33.0 41.0 36-37 37.9474332392023 40.0 37.5 41.0 33.0 41.0 38-39 37.75450974477552 40.0 37.0 41.0 33.0 41.0 40-41 37.5326291178896 40.0 37.0 41.0 33.0 41.0 42-43 37.26698090577871 40.0 36.5 41.0 32.0 41.0 44-45 36.989111444899365 40.0 36.0 41.0 31.0 41.0 46-47 36.60451993687078 39.0 36.0 41.0 30.5 41.0 48-49 36.167500651161646 39.0 35.0 40.5 30.0 41.0 50-51 35.77049383275286 39.0 35.0 40.0 28.5 41.0 52-53 35.23833468370016 38.0 34.5 40.0 27.0 41.0 54-55 34.847790837615214 38.0 34.0 40.0 26.0 41.0 56-57 34.46254842532129 38.0 34.0 40.0 25.0 41.0 58-59 33.94924388036745 37.5 33.0 40.0 23.5 41.0 60-61 33.46888554071124 37.0 33.0 39.5 22.5 41.0 62-63 32.90417989151081 36.0 32.0 39.0 20.5 40.0 64-65 32.3289449843052 35.5 31.0 39.0 18.0 40.0 66-67 31.761348228891812 35.0 31.0 38.5 14.5 40.0 68-69 31.122425852507 35.0 30.0 38.0 8.0 40.0 70-71 30.49032626029376 34.5 29.0 37.0 7.0 39.5 72-73 29.810495232879084 34.0 28.5 36.5 2.0 39.0 74-75 29.14378035108165 34.0 28.0 36.0 2.0 39.0 76-77 27.854488022229063 31.5 26.5 34.5 2.0 36.5 78-79 28.50028774652774 33.5 27.5 35.0 2.0 37.0 80-81 28.21011544864265 33.5 27.0 35.0 2.0 37.0 82-83 27.6353880871908 33.0 26.0 35.0 2.0 36.5 84-85 26.961160138406594 33.0 25.5 35.0 2.0 36.0 86-87 26.23179681491876 32.0 24.0 35.0 2.0 35.5 88-89 25.40615216489369 32.0 20.5 34.0 2.0 35.0 90-91 24.72045090241223 31.0 18.5 34.0 2.0 35.0 92-93 23.759967405885263 31.0 6.0 34.0 2.0 35.0 94-95 22.83136998232341 30.0 2.0 34.0 2.0 35.0 96-97 21.748993401905203 29.5 2.0 34.0 2.0 35.0 98-99 20.30701550742736 29.0 2.0 34.0 2.0 35.0 100-101 16.501684269479 13.5 2.0 31.0 2.0 33.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.16589189330279197 1101 2 0.15684783918310785 1101 3 0.13635513441986546 1101 4 0.07568962793256873 1101 5 -0.005817672998780665 1101 6 0.06379613115885974 1101 7 0.052974667602569525 1101 8 0.16059446665683197 1101 9 0.018924772971622872 1101 10-11 0.13255728652737986 1101 12-13 0.20913746886729 1101 14-15 0.3180457796253293 1101 16-17 0.3652618151258977 1101 18-19 0.30211095907884555 1101 20-21 0.23918491522142205 1101 22-23 0.3301011715338191 1101 24-25 0.2913785408202543 1101 26-27 0.3580127579541781 1101 28-29 0.4071996081477707 1101 30-31 0.32312381062750717 1101 32-33 0.3574157807469831 1101 34-35 0.3122996803718081 1101 36-37 0.3218718696170413 1101 38-39 0.4618636392992812 1101 40-41 0.48791638762925515 1101 42-43 0.6213356885036703 1101 44-45 0.6224698406570752 1101 46-47 0.5345829429945255 1101 48-49 0.5667516210677874 1101 50-51 0.6866619126324665 1101 52-53 0.8244635705327354 1101 54-55 0.9589180885329185 1101 56-57 0.972611574681423 1101 58-59 1.0810048911252679 1101 60-61 0.9284703824915326 1101 62-63 0.9615088303287465 1101 64-65 1.226831014639444 1101 66-67 1.2508941155085829 1101 68-69 1.5257232984010756 1101 70-71 1.7263300595147193 1101 72-73 2.0114508909112665 1101 74-75 1.8885835917219929 1101 76-77 1.840312116635392 1101 78-79 2.058548230091823 1101 80-81 2.105889450697166 1101 82-83 2.2990114408473126 1101 84-85 2.586102926065749 1101 86-87 2.710497675862701 1101 88-89 3.1307991767243237 1101 90-91 3.2264724009063634 1101 92-93 3.308816380348894 1101 94-95 3.596459835254432 1101 96-97 3.8106202210778797 1101 98-99 4.211588775421738 1101 100-101 3.948911051866787 1102 1 0.16180805856420477 1102 2 0.1806866569961798 1102 3 0.19529210046028567 1102 4 0.07733908398051881 1102 5 -0.05208994838622516 1102 6 -0.012111744056198859 1102 7 0.1251082689368772 1102 8 0.13015321907064958 1102 9 0.12488867179358465 1102 10-11 0.15428592567680255 1102 12-13 0.2687575413065062 1102 14-15 0.42904933579204396 1102 16-17 0.3648295484232662 1102 18-19 0.28334469561985287 1102 20-21 0.2306119545892713 1102 22-23 0.29423652298901715 1102 24-25 0.31467620282980846 1102 26-27 0.2666052923068847 1102 28-29 0.2467834280827006 1102 30-31 0.3599057922775586 1102 32-33 0.2902229812443906 1102 34-35 0.4561007324181645 1102 36-37 0.49084390386941124 1102 38-39 0.5139369733124823 1102 40-41 0.6513084311585828 1102 42-43 0.6955126875726805 1102 44-45 0.6947523009586121 1102 46-47 0.5836646229790077 1102 48-49 0.6303533830316042 1102 50-51 0.7563984733817364 1102 52-53 0.9489157530848189 1102 54-55 1.1566805984431454 1102 56-57 1.0850716459363383 1102 58-59 1.2312223403569575 1102 60-61 1.0394316660012421 1102 62-63 1.2466527053990646 1102 64-65 1.12480357195426 1102 66-67 1.5333927420584423 1102 68-69 1.6761687239074377 1102 70-71 1.8045499365457012 1102 72-73 2.135776091007326 1102 74-75 2.2262593851156147 1102 76-77 1.9818991999536486 1102 78-79 2.1539984092160402 1102 80-81 2.2301934322222046 1102 82-83 2.3489194937062265 1102 84-85 2.5044375807622927 1102 86-87 2.6324126765699845 1102 88-89 2.688585734348372 1102 90-91 2.8653497575658697 1102 92-93 3.1071769578887896 1102 94-95 3.3320068631741258 1102 96-97 3.538082808954016 1102 98-99 3.930069657324765 1102 100-101 3.464352250107453 1103 1 0.1600798238005936 1103 2 0.20190614345077051 1103 3 0.20238860849222817 1103 4 0.08095097950610608 1103 5 -0.025629151875953937 1103 6 -0.01186293453619669 1103 7 0.07918884100688928 1103 8 0.15380387515384797 1103 9 0.19042791366010192 1103 10-11 0.3027403004709228 1103 12-13 0.2642148487269793 1103 14-15 0.40107089715173316 1103 16-17 0.3867382180598682 1103 18-19 0.26265720741272247 1103 20-21 0.3226027185752116 1103 22-23 0.3654420978573327 1103 24-25 0.38929379411059983 1103 26-27 0.3003693518037025 1103 28-29 0.42284478617333576 1103 30-31 0.31082843426368356 1103 32-33 0.5180458339830523 1103 34-35 0.5718035553508543 1103 36-37 0.6345990407626161 1103 38-39 0.5753107344617376 1103 40-41 0.55468574855702 1103 42-43 0.5685378019834886 1103 44-45 0.5531472237093311 1103 46-47 0.857514358658868 1103 48-49 0.9604909958033971 1103 50-51 0.8676174481458006 1103 52-53 0.9554226976847247 1103 54-55 1.1643749848782292 1103 56-57 1.1414561046157345 1103 58-59 1.3428545052236487 1103 60-61 1.26909660464635 1103 62-63 1.3922364963223046 1103 64-65 1.4106917867619444 1103 66-67 1.593302588583267 1103 68-69 1.7231612628199002 1103 70-71 1.7167755025233937 1103 72-73 2.041864088986145 1103 74-75 2.1725090133404628 1103 76-77 2.2281979184886787 1103 78-79 2.2300968648700596 1103 80-81 2.3048909751482434 1103 82-83 2.552216379749545 1103 84-85 2.8448868770279105 1103 86-87 3.0433116848497797 1103 88-89 3.1036479846993608 1103 90-91 3.2642221195613352 1103 92-93 3.3625385152189153 1103 94-95 3.6485218349192117 1103 96-97 3.7766385304536563 1103 98-99 4.162807937213088 1103 100-101 4.104170408085508 1104 1 0.18420172181809846 1104 2 0.18157762182828208 1104 3 0.1547659642466428 1104 4 0.06873700842470498 1104 5 -0.0638393525418195 1104 6 0.09776582454995975 1104 7 0.116059154422679 1104 8 0.05156277399915865 1104 9 0.11141897705021364 1104 10-11 0.19893876692692203 1104 12-13 0.19622017806431558 1104 14-15 0.3317928350486383 1104 16-17 0.34348810778729444 1104 18-19 0.2114371094060843 1104 20-21 0.29386236364859286 1104 22-23 0.24751721613599642 1104 24-25 0.35873786930138607 1104 26-27 0.34670558344256364 1104 28-29 0.33002201987262936 1104 30-31 0.29495190328766796 1104 32-33 0.3070607222905366 1104 34-35 0.3477946489526289 1104 36-37 0.5463695046324872 1104 38-39 0.596089409861591 1104 40-41 0.5024201759553719 1104 42-43 0.6575943823857884 1104 44-45 0.7449050288924468 1104 46-47 0.9228641492920389 1104 48-49 0.9120819419722608 1104 50-51 1.0045166136996713 1104 52-53 1.0938463437490569 1104 54-55 1.2406015783923863 1104 56-57 1.3154100257874646 1104 58-59 1.372273716215858 1104 60-61 1.4703790897369018 1104 62-63 1.559783883315582 1104 64-65 1.7307082219065677 1104 66-67 1.672414698021992 1104 68-69 1.7529436180003017 1104 70-71 1.8784190774090384 1104 72-73 2.1817554176346583 1104 74-75 2.050117941491088 1104 76-77 2.0996531840794077 1104 78-79 2.3714283834895085 1104 80-81 2.4379183650418383 1104 82-83 2.6691229773724956 1104 84-85 2.7225530562797466 1104 86-87 2.7193340475226613 1104 88-89 3.059531252876205 1104 90-91 3.239214415587295 1104 92-93 3.417245794416978 1104 94-95 3.7211038444781828 1104 96-97 4.2594193931779 1104 98-99 4.3254217596531035 1104 100-101 3.9398802915289153 1105 1 0.17374569969936005 1105 2 0.18871765886781589 1105 3 0.19540503878327797 1105 4 0.10598193374657683 1105 5 -0.025003473263758735 1105 6 -0.021927714984549596 1105 7 0.016599000445033596 1105 8 0.0756339045035972 1105 9 0.13852823910089995 1105 10-11 0.17055463070907706 1105 12-13 0.2393191407064208 1105 14-15 0.42265065505660715 1105 16-17 0.35903164521079844 1105 18-19 0.38229150046182525 1105 20-21 0.3391707468377163 1105 22-23 0.3299766706302094 1105 24-25 0.2880143033279978 1105 26-27 0.33279122623132906 1105 28-29 0.3107447186234893 1105 30-31 0.40052569080216927 1105 32-33 0.4955496840311824 1105 34-35 0.4619185659229288 1105 36-37 0.543248644621606 1105 38-39 0.620458648109306 1105 40-41 0.6301021471978174 1105 42-43 0.711420231550683 1105 44-45 0.8322886812914021 1105 46-47 0.8617713176849762 1105 48-49 1.0794807080058888 1105 50-51 1.1395726864824596 1105 52-53 1.2230030211913459 1105 54-55 1.34365286091181 1105 56-57 1.37130537028591 1105 58-59 1.511148278182901 1105 60-61 1.5506077283915118 1105 62-63 1.5317493461784935 1105 64-65 1.7507941225799186 1105 66-67 1.783058456701351 1105 68-69 2.0702007186699944 1105 70-71 2.1716381389601658 1105 72-73 2.268224207322927 1105 74-75 2.0753147496557602 1105 76-77 2.226148857665976 1105 78-79 2.0933935610818466 1105 80-81 2.2937106698112224 1105 82-83 2.428484844228734 1105 84-85 2.720544608847696 1105 86-87 2.7632339072836523 1105 88-89 2.93377785330685 1105 90-91 3.1969792522242493 1105 92-93 3.4846740574273127 1105 94-95 3.608405139264235 1105 96-97 3.7990625881626165 1105 98-99 3.679567625340873 1105 100-101 3.481143823338293 1106 1 0.2644159329062745 1106 2 0.23993938841535822 1106 3 0.22964310755251915 1106 4 0.1712458115421569 1106 5 0.14731539795495507 1106 6 0.15181195812062498 1106 7 0.23385362922880404 1106 8 0.19725294523600923 1106 9 0.32954964331020165 1106 10-11 0.3295566581392748 1106 12-13 0.27582778778177186 1106 14-15 0.28100846419554415 1106 16-17 0.2752363488709406 1106 18-19 0.1889112008128322 1106 20-21 0.21232057188699827 1106 22-23 0.19529373189966037 1106 24-25 0.16395481180285998 1106 26-27 0.08236054220960654 1106 28-29 0.2823557280165616 1106 30-31 0.3216463469101569 1106 32-33 0.3488510612816853 1106 34-35 0.2709861688248054 1106 36-37 0.2948108919173009 1106 38-39 0.3256112076778308 1106 40-41 0.4013897502873718 1106 42-43 0.42549282161070323 1106 44-45 0.5824237789066729 1106 46-47 0.7634136299820895 1106 48-49 0.8673152160491 1106 50-51 0.8700380404989261 1106 52-53 0.9698626571150015 1106 54-55 0.9364680965005192 1106 56-57 0.9259560125446598 1106 58-59 1.219202469576068 1106 60-61 1.2794806140931385 1106 62-63 1.1926173490465928 1106 64-65 1.3407380853586588 1106 66-67 1.5648798099075059 1106 68-69 1.5291816879836588 1106 70-71 1.3329751886048768 1106 72-73 1.7314588885781141 1106 74-75 2.079869291833539 1106 76-77 2.08789928669405 1106 78-79 2.253870346647293 1106 80-81 2.0716916076619007 1106 82-83 2.45075814932634 1106 84-85 2.4739179093638555 1106 86-87 2.6387724348052046 1106 88-89 2.818201498453025 1106 90-91 2.744866733988143 1106 92-93 2.8276508857713694 1106 94-95 3.2150344165044125 1106 96-97 3.524224222316615 1106 98-99 3.8028645536157697 1106 100-101 3.629364122700256 1107 1 0.14486071304234116 1107 2 0.16468250828599196 1107 3 0.17878723472331615 1107 4 0.13132469907363742 1107 5 -0.010909131302312858 1107 6 0.033078506844624656 1107 7 0.08151384171709708 1107 8 0.1658514158114599 1107 9 0.1521301431189812 1107 10-11 0.18561648334307534 1107 12-13 0.18153617877689499 1107 14-15 0.3923082610345219 1107 16-17 0.2029528178681872 1107 18-19 0.2046906784518896 1107 20-21 0.2620375714572418 1107 22-23 0.23602239112141632 1107 24-25 0.2057685547700956 1107 26-27 0.24392311912719578 1107 28-29 0.2866070709238713 1107 30-31 0.2897981673894918 1107 32-33 0.36249416588849215 1107 34-35 0.3816020503053821 1107 36-37 0.4384015756862212 1107 38-39 0.3614903620728853 1107 40-41 0.32443188240229404 1107 42-43 0.40698120279417793 1107 44-45 0.5363912201986594 1107 46-47 0.5130166859023362 1107 48-49 0.44880140059275675 1107 50-51 0.43451153128064846 1107 52-53 0.5388323598195086 1107 54-55 0.7447884612103195 1107 56-57 0.7523857012019448 1107 58-59 0.9030485493343647 1107 60-61 0.7978105876940305 1107 62-63 0.847170955225117 1107 64-65 1.0316893092531458 1107 66-67 1.1156664040963271 1107 68-69 1.4137247502910668 1107 70-71 1.3952216245567755 1107 72-73 1.6065858290136852 1107 74-75 1.5047429428009913 1107 76-77 1.4101681734313622 1107 78-79 1.284642876708876 1107 80-81 1.378938435066278 1107 82-83 1.6931964983769134 1107 84-85 1.8636663039862533 1107 86-87 1.7679033145481853 1107 88-89 2.021589364777917 1107 90-91 2.2857721100669615 1107 92-93 2.4555676995847406 1107 94-95 2.4254802555335218 1107 96-97 2.4024434965355574 1107 98-99 2.881376544171342 1107 100-101 2.9432694441447005 1108 1 0.16067184068066354 1108 2 0.15040826484459302 1108 3 0.1510440470780665 1108 4 0.08375471073043883 1108 5 0.039516992803868334 1108 6 0.09700896970886674 1108 7 0.16376376529703407 1108 8 0.1817713597652073 1108 9 0.22400741469613195 1108 10-11 0.17095582497511685 1108 12-13 0.22317289901805282 1108 14-15 0.3098464089047823 1108 16-17 0.2909240810003695 1108 18-19 0.19705642412885283 1108 20-21 0.2787069439059451 1108 22-23 0.29429001782879993 1108 24-25 0.19381133830314212 1108 26-27 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2316 60-61 0.3341198994426122 2316 62-63 0.35396832268363454 2316 64-65 0.609556627642462 2316 66-67 0.8699954670312628 2316 68-69 1.0771090528955796 2316 70-71 1.2069497821102502 2316 72-73 1.2198776221535006 2316 74-75 1.5681433959667395 2316 76-77 1.7990849695400257 2316 78-79 1.8467902363296211 2316 80-81 1.7770795767209506 2316 82-83 1.958006800534914 2316 84-85 2.064853845253314 2316 86-87 2.01829935956075 2316 88-89 2.496204126390886 2316 90-91 2.6607580970800093 2316 92-93 2.4643879248375704 2316 94-95 3.172078543574031 2316 96-97 3.5178456692610602 2316 98-99 3.7792504890409298 2316 100-101 3.9154653029507926 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 11.0 4 18.0 5 37.0 6 85.0 7 207.0 8 508.0 9 916.0 10 1694.0 11 2755.0 12 4374.0 13 6540.0 14 8748.0 15 12077.0 16 15744.0 17 21969.0 18 22887.0 19 18373.0 20 17647.0 21 18778.0 22 20685.0 23 22946.0 24 25806.0 25 29021.0 26 32592.0 27 38172.0 28 45478.0 29 54541.0 30 65205.0 31 77974.0 32 94337.0 33 116872.0 34 152141.0 35 205964.0 36 280822.0 37 341284.0 38 177922.0 39 7550.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.80480181522246 18.664351654053522 14.182815303791358 23.348031226932665 2 15.804029905610545 19.92933404311622 40.29128212943104 23.975353921842196 3 19.697584298351096 23.706080023720492 29.68196624771443 26.91436943021398 4 12.68597019535171 16.31235683216225 36.497464803232695 34.50420816925334 5 13.177144724343787 37.88284153523676 34.41913227137998 14.520881469039473 6 32.85310352034122 35.95608337138213 16.826746130733763 14.364066977542892 7 29.094056567158187 32.82544441138591 19.83721473715204 18.243284284303865 8 26.739648433579028 35.32398849628631 19.037750485541345 18.898612584593323 9 26.482306419681656 16.394757350920017 19.941349124560787 37.18158710483754 10-11 25.268958069308304 26.555529932330664 26.84301908392624 21.332492914434788 12-13 26.032979149443914 23.85115011103207 27.537033853198828 22.578836886325192 14-15 22.475363293140585 25.426183049657418 26.034363344873228 26.064090312328762 16-17 21.813431589274927 28.709569736469927 27.036650114802658 22.440348559452488 18-19 21.783107065386925 26.903991492174224 29.62749436088871 21.685407081550146 20-21 22.328667275447035 26.0249490137861 30.084259801655648 21.562123909111218 22-23 21.94178695401623 26.215534305927214 29.873789973564797 21.968888766491762 24-25 21.765254247757728 26.268613538466607 30.194700906792292 21.77143130698337 26-27 21.38172787926039 26.673146087341937 29.86174690569483 22.083379127702845 28-29 21.473637991189502 27.016413391383665 29.780710378744434 21.7292382386824 30-31 21.66620682129139 26.524078567670877 29.761818969857135 22.047895641180595 32-33 21.273476564872688 26.523846929142287 29.82225088820507 22.380425617779956 34-35 21.99871709135988 26.789830014605183 29.64036343586821 21.57108945816673 36-37 22.096387129851454 26.296079717254532 29.572505000859646 22.035028152034368 38-39 21.638513678733165 26.685768794584003 29.612399429407155 22.06331809727568 40-41 22.17474008808305 26.44146544246159 29.651168477491463 21.732625991963904 42-43 21.676097491668237 26.507439129481313 29.510168509075108 22.30629486977534 44-45 21.73657783762859 26.843089290273692 29.56311824104503 21.85721463105269 46-47 22.154423626718113 26.989646272524276 28.805666599062533 22.05026350169508 48-49 21.947313044543574 27.135578545536532 28.803067100019458 22.114041309900436 50-51 22.14397415531724 27.38855355637207 28.577039371343325 21.890432916967367 52-53 22.389073456180682 27.110046832162958 28.59011407940157 21.91076563225479 54-55 21.91377693312647 27.313631361180057 28.67203690567988 22.10055480001359 56-57 22.019332556571936 27.556447106743953 28.31941314106301 22.104807195621103 58-59 21.94684976748639 27.152230781980784 28.419628122358674 22.481291328174144 60-61 21.73083397076825 27.40023843325876 28.65950268752168 22.209424908451307 62-63 21.856665167021674 27.294302412849863 28.83961451230824 22.009417907820218 64-65 21.57347419701217 27.356561701812236 28.608567948845977 22.461396152329616 66-67 21.496823975127143 27.53490024090432 28.4734490497663 22.494826734202235 68-69 22.087793694033152 27.133097369185062 28.56471100568908 22.2143979310927 70-71 21.878722302466382 27.23080771840167 28.211333609926896 22.679136369205047 72-73 21.823675162397954 27.394892060278536 28.214712499192483 22.56672027813103 74-75 21.78099658101532 27.725742041157535 27.985975059222252 22.507286318604898 76-77 21.807943863174724 27.369739360327628 28.319148476178807 22.503168300318837 78-79 21.887704729852853 27.47469735139359 28.207859031998034 22.429738886755526 80-81 21.983651450573436 27.559531163135464 27.973366689315792 22.483450696975314 82-83 21.933043402389472 27.643423546177853 28.11535706573481 22.30817598569786 84-85 21.89040064223421 27.666416056678866 27.95075243482489 22.492430866262037 86-87 21.990744970901403 27.653639840431953 27.83378319840813 22.521831990258516 88-89 21.975547207417375 27.785787895291147 27.528463227640966 22.710201669650516 90-91 21.914587770492435 27.638403973978676 27.78719653049012 22.659811725038768 92-93 21.969704768974026 27.458945931449406 27.772558761547177 22.798790538029383 94-95 21.797479874462148 27.330349141212583 27.918170816515936 22.954000167809333 96-97 22.052273676640947 27.02622846434525 27.82677224935971 23.094725609654088 98-99 21.709626176595037 27.24717684108876 27.57479093335914 23.46840604895706 100-101 22.081946504883454 26.653538767538898 27.872832506812745 23.3916822207649 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1228.0 1 815.5 2 589.5 3 761.0 4 603.5 5 557.5 6 1053.0 7 2222.0 8 3478.0 9 3865.0 10 3114.5 11 2301.5 12 2150.5 13 2188.0 14 2378.0 15 2572.5 16 2685.5 17 2886.0 18 3135.5 19 3488.5 20 3898.5 21 4569.5 22 5582.0 23 6698.5 24 8144.0 25 10059.5 26 12702.5 27 15718.0 28 18002.5 29 20306.0 30 23374.0 31 27638.5 32 33945.0 33 37441.0 34 38724.0 35 41786.0 36 46025.0 37 50227.0 38 52632.0 39 54247.5 40 56742.0 41 59608.5 42 60837.0 43 62193.5 44 64554.0 45 67956.5 46 69441.5 47 70279.0 48 71692.5 49 71048.0 50 69570.0 51 67519.5 52 65636.5 53 65323.0 54 65049.0 55 60840.0 56 55384.5 57 50731.0 58 46497.5 59 41045.0 60 33368.5 61 27329.0 62 22742.5 63 16891.5 64 11809.5 65 8785.5 66 6502.5 67 5133.5 68 4047.0 69 3072.0 70 2388.5 71 1597.0 72 1126.0 73 882.0 74 633.0 75 463.5 76 306.5 77 192.5 78 121.0 79 62.5 80 34.0 81 21.5 82 14.5 83 11.5 84 8.0 85 3.5 86 2.5 87 2.0 88 1.0 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.015648469487028756 3 0.0029855632573936443 4 0.0016472073144240798 5 0.12462152838189679 6 5.1475228575752494E-5 7 0.0 8 2.5737614287876245E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 7.721284286362874E-5 16-17 2.5737614287876247E-5 18-19 0.0 20-21 0.0 22-23 3.860642143181437E-4 24-25 5.147522857575249E-4 26-27 0.006254240271953928 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0113760255152413 36-37 8.236036572120399E-4 38-39 0.007258007229181101 40-41 0.010140620029423241 42-43 0.0059711265147872885 44-45 0.003809166914605684 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 2.5737614287876247E-5 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 1.0295045715150499E-4 68-69 7.206532000605349E-4 70-71 0.0 72-73 2.5737614287876247E-5 74-75 0.0 76-77 0.0 78-79 0.0 80-81 1.0295045715150499E-4 82-83 0.0018273706144392135 84-85 0.004555557728954095 86-87 0.0032172017859845308 88-89 0.0 90-91 0.002290647671620986 92-93 0.0 94-95 5.1475228575752494E-5 96-97 1.2868807143938122E-4 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1942682.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.505390151857604 #Duplication Level Percentage of deduplicated Percentage of total 1 80.15420671112699 47.696073426582544 2 11.914961890725836 14.180089119043119 3 3.468773854239604 6.192322246352715 4 1.4751895553038157 3.511269201451955 5 0.7959485961442097 2.368161587719227 6 0.45618857987304123 1.6287407656900306 7 0.2918570726035734 1.2156948281698268 8 0.2282800841389994 1.0867116376472032 9 0.15849349704280807 0.8488095640258125 >10 0.9417399759609609 10.324291937529411 >50 0.07205722225374432 2.878986979291399 >100 0.03728691118870427 3.9760947229704393 >500 0.003113409971099199 1.3340085850566417 >1k 0.00164318859585791 1.5711473690988065 >5k 2.594508309249332E-4 1.1875980293708863 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9415 0.48463927704070975 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8282 0.42631784306438214 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5380 0.2769367297375484 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 2528 0.1301293778395023 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 2516 0.12951167509659328 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 2167 0.11154682032365563 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2099 0.10804650478050447 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 2079 0.10701700020898942 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 5.1475228575752494E-5 0.0 0.0 0.0 0.0 3 5.1475228575752494E-5 0.0 0.0 0.0 0.0 4 5.1475228575752494E-5 0.0 0.0 0.0 0.0 5 1.0295045715150499E-4 0.0 0.0 0.0 5.1475228575752494E-5 6 1.0295045715150499E-4 0.0 0.0 0.0 5.1475228575752494E-5 7 1.0295045715150499E-4 0.0 0.0 0.0 5.1475228575752494E-5 8 1.0295045715150499E-4 0.0 0.0 0.0 5.1475228575752494E-5 9 1.0295045715150499E-4 0.0 0.0 0.0 5.1475228575752494E-5 10-11 1.5442568572725747E-4 0.0 0.0 0.0 7.721284286362874E-5 12-13 1.5442568572725747E-4 0.0 0.0 0.0 2.0590091430300997E-4 14-15 1.5442568572725747E-4 0.0 0.0 0.0 2.3163852859088622E-4 16-17 1.5442568572725747E-4 0.0 0.0 1.0295045715150499E-4 2.5737614287876245E-4 18-19 1.8016330001513372E-4 0.0 0.0 1.2868807143938122E-4 2.5737614287876245E-4 20-21 2.0590091430300997E-4 2.5737614287876247E-5 0.0 2.831137571666387E-4 2.5737614287876245E-4 22-23 2.0590091430300997E-4 1.0295045715150499E-4 0.0 3.8606421431814367E-4 2.831137571666387E-4 24-25 2.0590091430300997E-4 1.5442568572725747E-4 0.0 7.463908143484112E-4 3.0885137145451495E-4 26-27 2.0590091430300997E-4 1.5442568572725747E-4 0.0 0.001261143100105936 4.1180182860601995E-4 28-29 3.0885137145451495E-4 1.5442568572725747E-4 0.0 0.004375394428938962 4.1180182860601995E-4 30-31 3.0885137145451495E-4 1.5442568572725747E-4 0.0 0.015751419944180264 4.1180182860601995E-4 32-33 3.3458898574239117E-4 1.5442568572725747E-4 0.0 0.03968740123190517 4.1180182860601995E-4 34-35 3.860642143181437E-4 1.5442568572725747E-4 0.0 0.07276023559182615 4.1180182860601995E-4 36-37 4.632770571817724E-4 1.5442568572725747E-4 0.0 0.12822479438219947 4.1180182860601995E-4 38-39 5.662275143332775E-4 1.5442568572725747E-4 0.0 0.2120522041178124 4.6327705718177245E-4 40-41 5.662275143332775E-4 1.5442568572725747E-4 0.0 0.2999204192966219 5.662275143332775E-4 42-43 6.691779714847823E-4 1.5442568572725747E-4 0.0 0.3851891354323559 5.662275143332775E-4 44-45 7.721284286362873E-4 1.5442568572725747E-4 0.0 0.48734172654093666 6.177027429090299E-4 46-47 7.721284286362873E-4 1.5442568572725747E-4 0.0 0.6093122806511821 6.434403571969061E-4 48-49 7.721284286362873E-4 1.5442568572725747E-4 0.0 0.7366105209190181 7.206532000605349E-4 50-51 7.721284286362873E-4 1.5442568572725747E-4 0.0 0.8715270950160654 7.206532000605349E-4 52-53 7.721284286362873E-4 1.5442568572725747E-4 0.0 0.9959941977122349 7.206532000605349E-4 54-55 7.721284286362873E-4 1.5442568572725747E-4 0.0 1.1324292910522669 7.206532000605349E-4 56-57 8.750788857877923E-4 2.0590091430300997E-4 0.0 1.2858254722080094 7.206532000605349E-4 58-59 8.750788857877923E-4 2.5737614287876245E-4 0.0 1.4519617724362504 7.463908143484112E-4 60-61 8.750788857877923E-4 2.5737614287876245E-4 0.0 1.634209819208702 7.721284286362873E-4 62-63 8.750788857877923E-4 2.5737614287876245E-4 0.0 1.8066003597088973 7.721284286362873E-4 64-65 8.750788857877923E-4 2.5737614287876245E-4 0.0 1.9806381075235164 7.978660429241636E-4 66-67 9.265541143635448E-4 2.5737614287876245E-4 0.0 2.181777563183269 8.236036572120399E-4 68-69 9.265541143635448E-4 2.5737614287876245E-4 0.0 2.402734981844687 8.236036572120399E-4 70-71 9.265541143635448E-4 2.5737614287876245E-4 0.0 2.6313879471781796 8.236036572120399E-4 72-73 9.265541143635448E-4 2.5737614287876245E-4 0.0 2.863875817040565 8.750788857877923E-4 74-75 9.265541143635448E-4 2.5737614287876245E-4 0.0 3.087098145759316 9.265541143635448E-4 76-77 9.265541143635448E-4 2.5737614287876245E-4 0.0 3.331322367736974 9.52291728651421E-4 78-79 9.265541143635448E-4 2.5737614287876245E-4 0.0 3.6032660003026744 9.780293429392972E-4 80-81 9.265541143635448E-4 2.5737614287876245E-4 0.0 3.8914500674840244 0.0010037669572271735 82-83 9.265541143635448E-4 2.5737614287876245E-4 0.0 4.184292642851481 0.0010295045715150498 84-85 9.52291728651421E-4 2.5737614287876245E-4 0.0 4.475745387047391 0.0010295045715150498 86-87 9.780293429392972E-4 2.5737614287876245E-4 0.0 4.7740185990295885 0.0010295045715150498 88-89 9.780293429392972E-4 3.0885137145451495E-4 0.0 5.086267335570104 0.0010295045715150498 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4470 0.0 50.7602 1 GTATCAA 7805 0.0 40.65045 1 ATCAACG 10025 0.0 31.456326 3 TCAACGC 10060 0.0 31.344463 4 TATCAAC 10220 0.0 30.905062 2 CAACGCA 10245 0.0 30.82488 5 AACGCAG 10565 0.0 29.894644 6 ACGCAGA 12140 0.0 25.85973 7 CGCAGAG 12225 0.0 25.44683 8 TACATGG 8020 0.0 25.444586 2 GTACATG 8145 0.0 25.287697 1 ACATGGG 8195 0.0 23.912619 3 TGGTATC 2240 0.0 23.146797 2 GCAGAGT 13350 0.0 22.875519 9 GAGTACT 8005 0.0 22.21937 12-13 GTGGTAT 2635 0.0 20.399065 1 GTACTTT 8550 0.0 20.358656 14-15 AGAGTAC 12060 0.0 20.222477 10-11 CAGAGTA 12890 0.0 20.062553 10-11 AGTACTT 8410 0.0 19.68104 12-13 >>END_MODULE