FastQCFastQC Report
Thu 26 May 2016
SRR937938_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937938_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1924740
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98120.509783139540925No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83700.4348639296736182No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51600.26808815736151376No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA24770.12869270654737783No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA24250.12599104294606026No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA21390.11113189313881355No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA21260.11045647723848416No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21240.11035256709997195No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA45400.049.6031151
GTATCAA79050.041.223191
ATCAACG98750.032.4333463
TCAACGC100900.031.7893474
TATCAAC101100.031.7734642
CAACGCA101200.031.6481555
AACGCAG104950.030.877146
ACGCAGA118950.027.0341437
CGCAGAG119850.026.950038
GTACATG85750.025.8726921
TACATGG84100.025.7089542
ACATGGG86900.024.1697143
GCAGAGT131400.024.11129
GAGTACT77350.023.58237512-13
GTACTTT83800.021.40130814-15
CAGAGTA124800.021.1238210-11
AGTACTT81700.020.64062912-13
AGAGTAC118450.020.61209110-11
CATGGGG43150.020.2631074
TACCTGG13500.019.7116642
Produced by FastQC (version 0.11.5)