##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937938_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1924740 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.950121055311367 33.0 31.0 34.0 30.0 34.0 2 32.14149287696001 34.0 31.0 34.0 30.0 34.0 3 32.2265261801594 34.0 31.0 34.0 30.0 34.0 4 35.73492627575673 37.0 35.0 37.0 35.0 37.0 5 35.74792335588183 37.0 35.0 37.0 35.0 37.0 6 35.777415650945066 37.0 36.0 37.0 35.0 37.0 7 35.722451863628336 37.0 36.0 37.0 35.0 37.0 8 35.696740858505564 37.0 35.0 37.0 35.0 37.0 9 37.353967289088395 39.0 37.0 39.0 35.0 39.0 10-11 37.265911759510374 39.0 37.0 39.0 34.0 39.0 12-13 37.17046042582375 39.0 37.0 39.0 34.0 39.0 14-15 38.38484054989246 40.0 38.0 41.0 34.0 41.0 16-17 38.23483379573345 40.0 38.0 41.0 33.0 41.0 18-19 38.38726139634444 40.0 38.0 41.0 34.0 41.0 20-21 38.36628531645833 40.0 38.0 41.0 34.0 41.0 22-23 38.282687531822475 40.0 38.0 41.0 34.0 41.0 24-25 38.186265157891455 40.0 38.0 41.0 34.0 41.0 26-27 38.01663133721957 40.0 38.0 41.0 33.0 41.0 28-29 37.82062720159606 40.0 38.0 41.0 33.0 41.0 30-31 37.60239460914201 40.0 37.5 41.0 32.5 41.0 32-33 37.34900557997444 40.0 37.0 41.0 32.0 41.0 34-35 37.12131794424182 40.0 36.5 41.0 31.0 41.0 36-37 36.87064850317445 39.5 36.0 41.0 30.5 41.0 38-39 36.590453775574886 39.0 35.5 41.0 30.0 41.0 40-41 36.38458103432152 39.0 35.0 41.0 30.0 41.0 42-43 36.207957178631915 39.0 35.0 41.0 30.0 41.0 44-45 35.941929299541755 39.0 35.0 41.0 28.5 41.0 46-47 35.55648269376643 39.0 35.0 40.0 27.0 41.0 48-49 35.16396006733377 39.0 35.0 40.0 25.5 41.0 50-51 34.46123372507455 38.0 33.5 39.5 24.0 40.5 52-53 34.57730758440101 38.0 34.0 40.0 24.0 40.5 54-55 34.68953962613132 38.0 34.0 40.0 24.0 41.0 56-57 34.40418030487235 38.0 34.0 40.0 23.5 41.0 58-59 34.10104221868928 38.0 34.0 40.0 22.5 41.0 60-61 33.69983790018392 37.5 33.5 40.0 21.5 41.0 62-63 33.175638527801155 37.0 33.0 40.0 19.0 41.0 64-65 32.67136340492742 36.0 32.0 39.0 16.0 41.0 66-67 32.15249436287499 35.5 31.5 39.0 10.5 40.0 68-69 31.55960467387803 35.0 31.0 38.5 7.5 40.0 70-71 31.01889294138429 35.0 30.5 38.0 4.0 40.0 72-73 30.389037220611616 35.0 29.5 37.0 2.0 39.5 74-75 29.716220372621756 34.0 29.0 36.5 2.0 39.0 76-77 29.06036997204817 34.0 28.5 36.0 2.0 39.0 78-79 28.34443976848821 34.0 26.5 35.5 2.0 37.5 80-81 27.69510635202677 33.5 26.0 35.0 2.0 37.0 82-83 26.971842690441306 33.0 25.0 35.0 2.0 36.5 84-85 26.33034565707576 32.0 23.5 35.0 2.0 36.0 86-87 25.656244739549237 32.0 21.0 35.0 2.0 35.5 88-89 24.851761796398474 31.5 18.5 34.0 2.0 35.0 90-91 24.0350675935451 31.0 9.5 34.0 2.0 35.0 92-93 23.169949447717613 30.5 2.0 34.0 2.0 35.0 94-95 22.242730186934338 30.0 2.0 34.0 2.0 35.0 96-97 21.058634932510365 29.0 2.0 34.0 2.0 35.0 98-99 19.578843376248223 28.0 2.0 34.0 2.0 35.0 100-101 15.80556854432287 13.0 2.0 30.0 2.0 33.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.47267662268692945 1101 2 0.3505948666886667 1101 3 0.22976643864114266 1101 4 0.2116820560193915 1101 5 0.24029083535402407 1101 6 0.2054300242600533 1101 7 0.25624766979126434 1101 8 0.24526663515501923 1101 9 0.35739514392464855 1101 10-11 0.40797198685726954 1101 12-13 0.47185447916582035 1101 14-15 0.6124641663411694 1101 16-17 0.6709309404877146 1101 18-19 0.6343848070467928 1101 20-21 0.68532786831706 1101 22-23 0.6491854503240972 1101 24-25 0.6636756472312726 1101 26-27 0.7588956037881118 1101 28-29 0.7641266487864584 1101 30-31 0.7563751639992375 1101 32-33 0.7146597146944487 1101 34-35 0.8184727923684676 1101 36-37 0.917797549145817 1101 38-39 1.0481603781182685 1101 40-41 1.1711921554375309 1101 42-43 1.2143564549982884 1101 44-45 1.1041495832752304 1101 46-47 1.3686942118061367 1101 48-49 1.321600930840198 1101 50-51 1.2085678619525666 1101 52-53 1.178413970283927 1101 54-55 1.2528715107015245 1101 56-57 1.273122252379025 1101 58-59 1.3507764505165696 1101 60-61 1.3453022684917286 1101 62-63 1.3305014383193807 1101 64-65 1.3384333463698113 1101 66-67 1.411871515704128 1101 68-69 1.689820467272451 1101 70-71 1.952633152549236 1101 72-73 2.0065316106831865 1101 74-75 2.1189023237503832 1101 76-77 2.1911339903845963 1101 78-79 2.233051359575267 1101 80-81 2.570817940873301 1101 82-83 2.5722349469860113 1101 84-85 2.830955094161606 1101 86-87 3.052734708960113 1101 88-89 3.228545640635783 1101 90-91 3.3288653957782017 1101 92-93 3.5613023617094797 1101 94-95 3.8270332583517437 1101 96-97 3.849152938915374 1101 98-99 4.315314854243486 1101 100-101 4.486807727752108 1102 1 0.4552345549836083 1102 2 0.383045925528684 1102 3 0.2763126253052519 1102 4 0.22051033206879822 1102 5 0.26181486682484234 1102 6 0.29708799478289194 1102 7 0.09544951742990548 1102 8 0.3102935102341462 1102 9 0.4073687789373466 1102 10-11 0.4150029574206684 1102 12-13 0.48831938300429556 1102 14-15 0.502357157679441 1102 16-17 0.633413686477958 1102 18-19 0.5807480366778464 1102 20-21 0.5204621945472141 1102 22-23 0.49977217884094216 1102 24-25 0.44244144625017867 1102 26-27 0.5108416348434801 1102 28-29 0.5040280597127449 1102 30-31 0.6253829677896476 1102 32-33 0.7354084680423085 1102 34-35 0.7722778315840841 1102 36-37 0.8645851383043421 1102 38-39 0.8092007933698895 1102 40-41 0.9897239144922736 1102 42-43 1.2603307857025001 1102 44-45 1.130190692989224 1102 46-47 0.9950461087946252 1102 48-49 1.218148162270701 1102 50-51 1.1218977291197376 1102 52-53 1.1855663181198466 1102 54-55 1.2311578848581775 1102 56-57 1.2653080143025761 1102 58-59 1.3555667044439659 1102 60-61 1.3492690006713985 1102 62-63 1.2419451948568465 1102 64-65 1.418922764115358 1102 66-67 1.354489872372632 1102 68-69 1.4908631441360392 1102 70-71 1.7411727412062667 1102 72-73 1.818275461003811 1102 74-75 1.8334659985748303 1102 76-77 2.1011258289153325 1102 78-79 2.1784936583563344 1102 80-81 2.4545569508526626 1102 82-83 2.5964664598446276 1102 84-85 2.514085435529811 1102 86-87 2.612890514358238 1102 88-89 2.998252393145375 1102 90-91 3.2244775612839476 1102 92-93 3.3174677125850565 1102 94-95 3.328720248797662 1102 96-97 3.3583991215233304 1102 98-99 3.567272562099763 1102 100-101 3.761070890886849 1103 1 0.5430348143427501 1103 2 0.44915136413700907 1103 3 0.4004632018936789 1103 4 0.24626681474963874 1103 5 0.3460847130259168 1103 6 0.3351495781192497 1103 7 0.4056817469175087 1103 8 0.37592108445512196 1103 9 0.44699778712221416 1103 10-11 0.41200583202149943 1103 12-13 0.5263846735895825 1103 14-15 0.7207988304198167 1103 16-17 0.6815827133028733 1103 18-19 0.6510193109112024 1103 20-21 0.6628283387636387 1103 22-23 0.6624319890742782 1103 24-25 0.7580564158247824 1103 26-27 0.661140364962435 1103 28-29 0.712071640648297 1103 30-31 0.7160016631494273 1103 32-33 0.8364337818794638 1103 34-35 0.8216463388009814 1103 36-37 0.9448279853528803 1103 38-39 1.0524009573785875 1103 40-41 1.067856595448653 1103 42-43 1.131117960697253 1103 44-45 1.201350594791613 1103 46-47 1.0201528961001145 1103 48-49 1.1462021383322565 1103 50-51 1.0940898402422974 1103 52-53 1.156217325861327 1103 54-55 1.253738182016427 1103 56-57 1.2735167902505395 1103 58-59 1.3780735980733567 1103 60-61 1.372552933676964 1103 62-63 1.4016781320582794 1103 64-65 1.6119573623867396 1103 66-67 1.7522118791213046 1103 68-69 1.8822863495865896 1103 70-71 2.052074314488568 1103 72-73 2.015004147473608 1103 74-75 2.1783817661718423 1103 76-77 2.308916946198604 1103 78-79 2.5408548126157022 1103 80-81 2.6162285938831467 1103 82-83 2.6883754631952392 1103 84-85 2.965355872666361 1103 86-87 2.981697462335898 1103 88-89 3.3092903931883804 1103 90-91 3.3249271393619466 1103 92-93 3.6981056040798066 1103 94-95 3.978193930666766 1103 96-97 4.254526825868748 1103 98-99 4.56414660980019 1103 100-101 4.405701237388577 1104 1 0.18945408813070586 1104 2 0.2702862715761256 1104 3 0.24690434208206113 1104 4 0.29819401476394347 1104 5 0.23229554088233328 1104 6 0.10428852327859772 1104 7 0.18954441217042728 1104 8 0.14903242569061348 1104 9 0.2494107925420579 1104 10-11 0.277173859493395 1104 12-13 0.30831941682441055 1104 14-15 0.436392427552704 1104 16-17 0.3844759200036165 1104 18-19 0.3642127611678987 1104 20-21 0.3848742430768297 1104 22-23 0.3592115427521634 1104 24-25 0.3222734955463764 1104 26-27 0.422312685950196 1104 28-29 0.4145445380013584 1104 30-31 0.38790853016183746 1104 32-33 0.3038313570469313 1104 34-35 0.35128923572772663 1104 36-37 0.365460515680482 1104 38-39 0.44661193101100594 1104 40-41 0.4374828244022453 1104 42-43 0.2919480862708994 1104 44-45 0.38494326500916287 1104 46-47 0.41224685148441154 1104 48-49 0.5803571119756796 1104 50-51 0.47815941115200644 1104 52-53 0.43822598853152783 1104 54-55 0.6408587346450005 1104 56-57 0.742540592996356 1104 58-59 0.638115467416803 1104 60-61 0.7320276174736833 1104 62-63 0.9569368638345637 1104 64-65 1.0362672986716248 1104 66-67 1.1355773244326102 1104 68-69 1.2164771483703198 1104 70-71 1.2526216965028318 1104 72-73 1.4429462785262395 1104 74-75 1.4839176098779632 1104 76-77 1.5820972216052311 1104 78-79 1.7261811514513958 1104 80-81 2.0093306040792136 1104 82-83 2.1008613841213837 1104 84-85 2.197369898793273 1104 86-87 2.0908192238050987 1104 88-89 2.441868338654402 1104 90-91 2.6299072120574145 1104 92-93 2.9038330194575295 1104 94-95 3.021709958463223 1104 96-97 3.0758185112731304 1104 98-99 3.3163963392206774 1104 100-101 3.5964745790834023 1105 1 0.19281190459237152 1105 2 0.195243861852596 1105 3 0.15338862160317746 1105 4 0.16726298706031173 1105 5 0.28365032127393874 1105 6 0.233674154843996 1105 7 0.3506461326776815 1105 8 0.23175722762656648 1105 9 0.30105438467097656 1105 10-11 0.2296007394551296 1105 12-13 0.21685653977308306 1105 14-15 0.27048097824474127 1105 16-17 0.4348900354999401 1105 18-19 0.45404410524705696 1105 20-21 0.40755635154437897 1105 22-23 0.33540965544347046 1105 24-25 0.34753540219026746 1105 26-27 0.3925116215015976 1105 28-29 0.5649338764907412 1105 30-31 0.569139162041381 1105 32-33 0.5650800675278589 1105 34-35 0.667514527123565 1105 36-37 0.8870215785887439 1105 38-39 0.8346350602709762 1105 40-41 0.8665073399585523 1105 42-43 0.8414879129027852 1105 44-45 0.9345618815097367 1105 46-47 0.9461799505255541 1105 48-49 0.8771365064236036 1105 50-51 1.0897572798227202 1105 52-53 1.1142478753943479 1105 54-55 1.1779203741633637 1105 56-57 1.247720710182982 1105 58-59 1.2376062973907977 1105 60-61 1.3261701100912475 1105 62-63 1.2858138827299328 1105 64-65 1.4528473218170959 1105 66-67 1.6465536905121283 1105 68-69 1.8517748068671658 1105 70-71 1.7831589775379406 1105 72-73 1.9390080518100383 1105 74-75 1.8799762923694843 1105 76-77 2.0847525161830696 1105 78-79 2.2560751930119096 1105 80-81 2.23382523420765 1105 82-83 2.562426622132655 1105 84-85 2.606189246544897 1105 86-87 2.6371616004926217 1105 88-89 2.905095764216977 1105 90-91 3.204967102491363 1105 92-93 3.399538338255013 1105 94-95 3.7126993869402654 1105 96-97 3.7373357153323035 1105 98-99 3.713716419642079 1105 100-101 3.7776649293259013 1106 1 -0.012398537433405465 1106 2 -0.05043560879043696 1106 3 -0.06722252306228427 1106 4 -0.05406801836755193 1106 5 0.06675684428938666 1106 6 -0.1357238470150861 1106 7 -0.004058620189972828 1106 8 0.10492178119626061 1106 9 0.026583709381526432 1106 10-11 0.042413615855956266 1106 12-13 0.07986822777089486 1106 14-15 0.07221566650110844 1106 16-17 0.021784003806210706 1106 18-19 -0.02790391589268637 1106 20-21 -0.06399525237289083 1106 22-23 -0.08827679097541363 1106 24-25 -0.08584174479880602 1106 26-27 0.040771176344271964 1106 28-29 -0.02908127904732538 1106 30-31 -0.12361552995697167 1106 32-33 0.05371986567900677 1106 34-35 0.08118123630527663 1106 36-37 0.11596869103144058 1106 38-39 -0.055118425259593096 1106 40-41 0.18441879584407417 1106 42-43 0.17785619178295775 1106 44-45 0.31502665696826426 1106 46-47 0.4063150242957789 1106 48-49 0.37932115108137054 1106 50-51 0.30680063474609653 1106 52-53 0.5055745829978733 1106 54-55 -0.007202825998618323 1106 56-57 0.44544246625613937 1106 58-59 0.6208934431738911 1106 60-61 0.7377714069820769 1106 62-63 0.4722107972295362 1106 64-65 0.5679922890201325 1106 66-67 0.6994445977352015 1106 68-69 0.41717543506416277 1106 70-71 0.8112184105923781 1106 72-73 1.0336642829357139 1106 74-75 1.0838586768977159 1106 76-77 1.2156135748672305 1106 78-79 1.388799055813049 1106 80-81 1.3478039326815257 1106 82-83 1.369845381589979 1106 84-85 1.814136946092237 1106 86-87 1.6133229435974634 1106 88-89 1.8420372269561796 1106 90-91 1.9326339248259536 1106 92-93 2.0996684392538896 1106 94-95 1.9859271175351658 1106 96-97 2.0259138997546238 1106 98-99 2.1447192171981726 1106 100-101 2.3191741703926567 1107 1 0.19058529655512402 1107 2 0.030208585470305138 1107 3 0.019704802792396947 1107 4 -0.005792540015924885 1107 5 0.13188830584954303 1107 6 0.2140186786508096 1107 7 0.20001617015646644 1107 8 0.18640343854819008 1107 9 0.284639854902494 1107 10-11 0.176859177877148 1107 12-13 0.16795114237267939 1107 14-15 0.3232201665462 1107 16-17 0.29436121253117875 1107 18-19 0.18241287634548087 1107 20-21 0.3816760083496078 1107 22-23 0.33245919806561375 1107 24-25 0.31880641518062447 1107 26-27 0.2547013867940464 1107 28-29 0.3579547347434797 1107 30-31 0.35287338924558753 1107 32-33 0.34379386556772573 1107 34-35 0.32461475343921364 1107 36-37 0.33688207739451315 1107 38-39 0.528740600827426 1107 40-41 0.38515488561090194 1107 42-43 0.615550088950549 1107 44-45 0.5558291921887246 1107 46-47 0.6863783184707515 1107 48-49 0.6636742851886623 1107 50-51 0.485853097539902 1107 52-53 0.5828311527183487 1107 54-55 0.6478134628023966 1107 56-57 0.7413344603775869 1107 58-59 0.7400417537623767 1107 60-61 0.7702668695245478 1107 62-63 0.6859022635477885 1107 64-65 0.7988810071548045 1107 66-67 0.9488794540426468 1107 68-69 1.0217858948150926 1107 70-71 1.1208271004470767 1107 72-73 1.2413252715450653 1107 74-75 1.3517146035079364 1107 76-77 1.6119243964636105 1107 78-79 1.5846199662975486 1107 80-81 1.6598062992582108 1107 82-83 1.8278875693927539 1107 84-85 1.8027544508934987 1107 86-87 1.8335310095277784 1107 88-89 2.168630085592792 1107 90-91 2.2847872745823885 1107 92-93 2.7081912570054207 1107 94-95 3.226930264976634 1107 96-97 3.307513502223461 1107 98-99 3.142256351443603 1107 100-101 3.362524516793485 1108 1 0.2474141308340485 1108 2 0.14784141793979444 1108 3 0.0957445448938472 1108 4 0.14149630963226656 1108 5 0.020614373114632656 1108 6 0.0372052953945925 1108 7 0.10305487128953672 1108 8 0.12061055855386371 1108 9 0.13881844365458562 1108 10-11 0.10640885005386025 1108 12-13 0.13480399795057707 1108 14-15 0.1915598083658736 1108 16-17 0.2516508524433263 1108 18-19 0.11278483178549692 1108 20-21 0.12621467133201492 1108 22-23 0.13718361395155654 1108 24-25 0.2974965390613207 1108 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3056.0 9 3764.0 10 4668.0 11 6125.0 12 7817.0 13 9881.0 14 11509.0 15 14354.0 16 17526.0 17 21998.0 18 21472.0 19 17347.0 20 17542.0 21 17967.0 22 19931.0 23 22388.0 24 24923.0 25 28314.0 26 32126.0 27 37001.0 28 42740.0 29 50047.0 30 60378.0 31 72119.0 32 88727.0 33 112657.0 34 149713.0 35 205110.0 36 274362.0 37 319134.0 38 182508.0 39 12435.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.53429250135842 18.29841629750494 13.858401619770468 23.30888958136617 2 15.630195932288313 19.568436486638902 40.72088029975348 24.080487281319307 3 19.631030261444536 23.81643001764796 29.45800746984382 27.094532251063686 4 12.49107179060113 16.014322563339604 36.74809401573444 34.746511630324825 5 13.188558853826237 37.78493278402021 34.37959700051322 14.646911361640328 6 32.73185128489378 35.70529026192885 17.125209417250677 14.437649035926691 7 28.999441231888767 32.92542394906114 20.003898382171396 18.071236436878696 8 26.726962100780106 35.06923960912093 19.233917766951787 18.96988052314718 9 26.682235995400934 16.380749614838443 19.98551875700412 36.95149563275651 10-11 25.228170733070193 26.47653760057718 26.932528204753687 21.362763461598938 12-13 26.12101236767667 23.914956499501532 27.47735572689858 22.486675405923222 14-15 22.588082550120347 25.384416681955845 26.031733574356597 25.995767193567215 16-17 21.851084260326427 28.65287687288121 27.057508871457102 22.43852999533526 18-19 21.856438538447744 26.869888166666232 29.61935602920194 21.654317265684085 20-21 22.43322595284373 26.000408589953988 30.046826695999197 21.519538761203084 22-23 22.027226867168963 26.178336499751797 29.83522495432301 21.959211678756237 24-25 21.767112975322878 26.243967970586674 30.153570451920007 21.83534860217044 26-27 21.39692987643141 26.634812598948308 29.86390326607729 22.104354258542987 28-29 21.463747546962782 27.057283145792344 29.74023209740887 21.738737209836007 30-31 21.647742438170415 26.50844800534759 29.74135544932795 22.102454107154042 32-33 21.220064500350986 26.602041566835105 29.824200288429726 22.353693644384183 34-35 22.059383089542465 26.777074301635324 29.558031583653015 21.6055110251692 36-37 22.10943562752321 26.280856120077612 29.531953246782948 22.07775500561623 38-39 21.6074882628864 26.679418034567448 29.590934177580948 22.122159524965205 40-41 22.14989189426555 26.455750702797705 29.619256306341068 21.775101096595677 42-43 21.70022621318709 26.48177952295157 29.53022697710481 22.287767286756523 44-45 21.739924555792935 26.864664191697603 29.528204800442303 21.867206452067155 46-47 22.19625791813111 27.02768857871463 28.792193004656948 21.983860498497314 48-49 21.92330838477393 27.102299833803308 28.842246181061892 22.13214560036087 50-51 22.150108798025396 27.433185021597218 28.56845182033711 21.848254360040272 52-53 22.333815723494308 27.129172306820927 28.63950385695616 21.897508112728605 54-55 21.88681843454945 27.287248691820437 28.775117827096853 22.05081504653326 56-57 21.95092294553781 27.506471284074756 28.413737127256507 22.128868643130932 58-59 21.963291239547463 27.0706388373957 28.538521594878596 22.42754832817824 60-61 21.673333168781802 27.280028164293448 28.86660803435836 22.180030632566393 62-63 21.807772091805134 27.234668659297533 28.980983400747444 21.97657584814989 64-65 21.61610225176176 27.294183487911855 28.74103011988247 22.34868414044392 66-67 21.532568901248386 27.409374373235316 28.628324515760955 22.429732209755343 68-69 22.14081857731694 27.01650690634828 28.69260677137798 22.1500677449568 70-71 21.883858321931456 27.079008371999247 28.486180822920083 22.550952483149214 72-73 21.838912325763932 27.252367121492448 28.441416487371757 22.467304065371863 74-75 21.84486287107015 27.546207828641904 28.25860639563478 22.35032290465317 76-77 21.954714147834366 27.259661661607048 28.359008133059504 22.42661605749908 78-79 22.0663251387771 27.333151813286538 28.229805418477323 22.37071762945904 80-81 22.174623477561166 27.38567272589333 27.962703577518532 22.477000219026973 82-83 22.127627733736837 27.489254195436462 28.0794680307118 22.3036500401149 84-85 22.127164614494475 27.4718362717072 27.971601016468906 22.42939809732942 86-87 22.193487077757908 27.458530467104424 27.854339402265122 22.49364305287255 88-89 22.3011241093655 27.41525005326917 27.666134841153937 22.61749099621139 90-91 22.251990450721873 27.422160052532984 27.728559144691523 22.597290352053623 92-93 22.346210646543526 27.09772513040306 27.903501214393128 22.65256300866028 94-95 22.191879283785383 26.955833224192304 28.01200655261949 22.84028093940282 96-97 22.476122223657143 26.586890888364756 27.895364708884323 23.041622179093782 98-99 22.250255678983603 26.76231416503143 27.58628126351149 23.401148892473476 100-101 22.989324213755076 25.77678800232586 27.84339231660789 23.390495467311176 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1270.0 1 852.5 2 645.5 3 820.0 4 622.5 5 580.5 6 1111.5 7 2214.0 8 3425.0 9 3913.0 10 3184.5 11 2304.0 12 2135.5 13 2222.0 14 2343.5 15 2513.5 16 2686.0 17 2821.0 18 3067.0 19 3442.5 20 3912.0 21 4540.0 22 5456.5 23 6668.0 24 8043.5 25 9851.0 26 12542.0 27 15432.0 28 17729.5 29 20188.0 30 23124.0 31 27402.0 32 33455.0 33 36714.0 34 38319.0 35 41677.0 36 45717.0 37 49673.0 38 52083.0 39 53746.0 40 55944.0 41 58560.5 42 59974.0 43 61471.5 44 63847.5 45 67003.5 46 68503.0 47 69292.5 48 70684.5 49 69883.0 50 68636.0 51 66929.0 52 64908.0 53 64355.5 54 64164.5 55 60166.0 56 54625.5 57 49759.5 58 45831.5 59 40829.0 60 33347.5 61 27661.0 62 23092.0 63 17423.5 64 12327.5 65 9216.5 66 6929.0 67 5383.5 68 4338.5 69 3300.5 70 2482.0 71 1774.0 72 1238.5 73 914.0 74 655.5 75 472.0 76 358.5 77 241.0 78 131.5 79 81.5 80 56.5 81 38.0 82 27.5 83 14.5 84 9.0 85 9.5 86 7.0 87 5.0 88 5.0 89 4.0 90 2.0 91 3.0 92 3.0 93 1.5 94 2.0 95 1.5 96 0.5 97 1.0 98 1.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4630755322796845 2 0.039433897565385456 3 0.05221484460238786 4 0.05517628354998597 5 0.08369961657158889 6 0.08032253706994191 7 0.044941134906532836 8 0.03995344825794653 9 0.04530482039132558 10-11 0.04709727028066128 12-13 0.04436962914471565 14-15 0.03745960493365338 16-17 0.03805708823009861 18-19 0.04649978698421605 20-21 0.05460477778816879 22-23 0.04701933767677712 24-25 0.065359477124183 26-27 0.05566985670791899 28-29 0.04413583133306317 30-31 0.04590230368777082 32-33 0.045434708064465854 34-35 0.04987686648586303 36-37 0.04421376393694733 38-39 0.026081444766565872 40-41 0.024237039807974065 42-43 0.023327826095992186 44-45 0.014105801303033138 46-47 0.026860770805407483 48-49 0.02670490559763916 50-51 0.015716408449972463 52-53 0.015326745430551658 54-55 0.016106071469393267 56-57 0.014365576649313672 58-59 0.013897981026008706 60-61 0.016495734488814074 62-63 0.01641780188492991 64-65 0.016287914211789645 66-67 0.009040182050562673 68-69 0.012599104294606025 70-71 0.018132319170381456 72-73 0.01576836351922857 74-75 0.013274520194935418 76-77 0.009741575485520121 78-79 0.013040722383282936 80-81 0.01659964462732629 82-83 0.013923958560636761 84-85 0.012183463740557166 86-87 0.0038446751249519417 88-89 0.028575288090859022 90-91 0.03197834512713405 92-93 0.016729532300466556 94-95 0.03241996321581097 96-97 0.026678928063011108 98-99 0.023483691303760507 100-101 0.14898116109188772 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1924740.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.81534391970985 #Duplication Level Percentage of deduplicated Percentage of total 1 81.01692204399147 49.27071977421663 2 11.44445459206061 13.919968839793373 3 3.345959878866414 6.10457102324435 4 1.4197935584084196 3.4538093419838676 5 0.6862614025344207 2.0867611606976615 6 0.4372694319577216 1.5955614534051001 7 0.2876483780364605 1.224540452676384 8 0.20917212367421756 1.0176699711730899 9 0.15502034554448152 0.8484854065955915 >10 0.8927514393805019 10.030228571127772 >50 0.06683771037457877 2.779064884306319 >100 0.03310735894951371 3.6408916107825817 >500 0.003001085138314803 1.307151121753282 >1k 0.0015434152139904692 1.5033776215800498 >5k 2.5723586899841156E-4 1.2171987666639446 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9812 0.509783139540925 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8370 0.4348639296736182 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5160 0.26808815736151376 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 2477 0.12869270654737783 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 2425 0.12599104294606026 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 2139 0.11113189313881355 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 2126 0.11045647723848416 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2124 0.11035256709997195 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.0391013851221464E-4 2 0.0 0.0 0.0 0.0 1.0391013851221464E-4 3 0.0 0.0 0.0 0.0 1.0391013851221464E-4 4 0.0 0.0 0.0 0.0 1.0391013851221464E-4 5 0.0 0.0 0.0 0.0 1.0391013851221464E-4 6 0.0 0.0 0.0 0.0 1.0391013851221464E-4 7 0.0 0.0 0.0 0.0 1.0391013851221464E-4 8 0.0 0.0 0.0 0.0 1.0391013851221464E-4 9 0.0 0.0 0.0 0.0 1.0391013851221464E-4 10-11 0.0 0.0 0.0 0.0 1.0391013851221464E-4 12-13 0.0 0.0 0.0 0.0 3.117304155366439E-4 14-15 0.0 0.0 0.0 0.0 4.1564055404885855E-4 16-17 2.597753462805366E-5 0.0 0.0 0.0 4.6759562330496587E-4 18-19 5.195506925610732E-5 0.0 0.0 0.0 4.935731579330195E-4 20-21 5.195506925610732E-5 0.0 0.0 7.793260388416098E-5 6.494383657013414E-4 22-23 5.195506925610732E-5 2.597753462805366E-5 0.0 2.3379781165248293E-4 6.754159003293951E-4 24-25 5.195506925610732E-5 5.195506925610732E-5 0.0 4.1564055404885855E-4 6.754159003293951E-4 26-27 5.195506925610732E-5 5.195506925610732E-5 0.0 0.001298876731402683 8.312811080977171E-4 28-29 5.195506925610732E-5 5.195506925610732E-5 0.0 0.004286293213628853 9.351912466099317E-4 30-31 5.195506925610732E-5 5.195506925610732E-5 0.0 0.015092947618899176 9.611687812379854E-4 32-33 5.195506925610732E-5 5.195506925610732E-5 0.0 0.037277762191257 0.0010391013851221464 34-35 5.195506925610732E-5 5.195506925610732E-5 0.0 0.07076280432681817 0.0010910564543782537 36-37 5.195506925610732E-5 5.195506925610732E-5 0.0 0.12705612186581045 0.0010910564543782537 38-39 5.195506925610732E-5 5.195506925610732E-5 0.0 0.21431466068144267 0.001143011523634361 40-41 5.195506925610732E-5 5.195506925610732E-5 0.0 0.3011575589430261 0.001143011523634361 42-43 5.195506925610732E-5 5.195506925610732E-5 0.0 0.3871691760965117 0.001143011523634361 44-45 5.195506925610732E-5 5.195506925610732E-5 0.0 0.49414466369483667 0.001143011523634361 46-47 1.0391013851221464E-4 5.195506925610732E-5 0.0 0.61675862713925 0.001143011523634361 48-49 1.0391013851221464E-4 5.195506925610732E-5 0.0 0.7463345698639816 0.001143011523634361 50-51 1.0391013851221464E-4 5.195506925610732E-5 0.0 0.8881199538638985 0.001143011523634361 52-53 1.0391013851221464E-4 5.195506925610732E-5 0.0 1.017124390826813 0.001143011523634361 54-55 1.0391013851221464E-4 5.195506925610732E-5 0.0 1.155091077236406 0.001143011523634361 56-57 1.5586520776832196E-4 5.195506925610732E-5 0.0 1.313346218190509 0.001143011523634361 58-59 1.5586520776832196E-4 5.195506925610732E-5 0.0 1.4797323274831926 0.001143011523634361 60-61 1.8184274239637561E-4 5.195506925610732E-5 2.597753462805366E-5 1.66435466608477 0.001143011523634361 62-63 2.0782027702442927E-4 5.195506925610732E-5 5.195506925610732E-5 1.842118935544541 0.001143011523634361 64-65 2.0782027702442927E-4 5.195506925610732E-5 5.195506925610732E-5 2.017311429076135 0.001143011523634361 66-67 2.0782027702442927E-4 5.195506925610732E-5 7.793260388416098E-5 2.216013591446117 0.0011689890582624145 68-69 2.0782027702442927E-4 5.195506925610732E-5 1.0391013851221464E-4 2.4427714912143976 0.0011949665928904683 70-71 2.0782027702442927E-4 1.0391013851221464E-4 1.0391013851221464E-4 2.6775044941134905 0.0011949665928904683 72-73 2.0782027702442927E-4 1.0391013851221464E-4 1.0391013851221464E-4 2.9093280131342416 0.0012469216621465756 74-75 2.0782027702442927E-4 1.0391013851221464E-4 1.0391013851221464E-4 3.1334621819050885 0.001298876731402683 76-77 2.0782027702442927E-4 1.298876731402683E-4 1.0391013851221464E-4 3.3727932084333467 0.001298876731402683 78-79 2.0782027702442927E-4 1.5586520776832196E-4 1.0391013851221464E-4 3.6384135000051954 0.001298876731402683 80-81 2.597753462805366E-4 1.5586520776832196E-4 1.0391013851221464E-4 3.918555233434126 0.001298876731402683 82-83 3.117304155366439E-4 1.5586520776832196E-4 1.0391013851221464E-4 4.209581553872211 0.001298876731402683 84-85 3.6368548479275123E-4 1.5586520776832196E-4 1.0391013851221464E-4 4.502348369130376 0.001298876731402683 86-87 4.1564055404885855E-4 1.8184274239637561E-4 1.0391013851221464E-4 4.805688040982158 0.001298876731402683 88-89 4.1564055404885855E-4 2.0782027702442927E-4 1.0391013851221464E-4 5.125523447322755 0.001298876731402683 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4540 0.0 49.603115 1 GTATCAA 7905 0.0 41.22319 1 ATCAACG 9875 0.0 32.433346 3 TCAACGC 10090 0.0 31.789347 4 TATCAAC 10110 0.0 31.773464 2 CAACGCA 10120 0.0 31.648155 5 AACGCAG 10495 0.0 30.87714 6 ACGCAGA 11895 0.0 27.034143 7 CGCAGAG 11985 0.0 26.95003 8 GTACATG 8575 0.0 25.872692 1 TACATGG 8410 0.0 25.708954 2 ACATGGG 8690 0.0 24.169714 3 GCAGAGT 13140 0.0 24.1112 9 GAGTACT 7735 0.0 23.582375 12-13 GTACTTT 8380 0.0 21.401308 14-15 CAGAGTA 12480 0.0 21.12382 10-11 AGTACTT 8170 0.0 20.640629 12-13 AGAGTAC 11845 0.0 20.612091 10-11 CATGGGG 4315 0.0 20.263107 4 TACCTGG 1350 0.0 19.711664 2 >>END_MODULE