FastQCFastQC Report
Thu 26 May 2016
SRR937938_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937938_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1924740
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97650.5073412512858879No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82050.4262913432463606No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53640.2786869914897597No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA25160.130718954248366No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA24780.12874466161663392No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA22160.11513243347153382No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA21140.10983301640741087No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20790.10801458898344711No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA51150.046.5312231
GTATCAA82350.036.3437161
ATCAACG100750.029.372373
TCAACGC102900.029.0348764
CAACGCA104000.028.7277745
TATCAAC103200.028.7248172
AACGCAG107650.027.7530066
TACATGG78000.024.6668012
ACGCAGA121650.024.5200277
GTACATG81000.024.5156631
CGCAGAG122550.024.3011978
ACATGGG78300.023.5984673
GAGTACT81700.022.32454312-13
GCAGAGT133500.021.9877419
GTACTTT87250.020.76837714-15
TAAGGTG9850.020.7356175
TAGCCCT14250.020.6662434
CATGGGG39850.020.5014634
GTAAGGT8850.019.8583494
TGGTATC27050.019.845572
Produced by FastQC (version 0.11.5)