##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937938_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1924740 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.46766524309777 33.0 31.0 34.0 31.0 34.0 2 32.69949447717614 34.0 31.0 34.0 31.0 34.0 3 32.661968369753836 34.0 31.0 34.0 31.0 34.0 4 36.19153444101541 37.0 35.0 37.0 35.0 37.0 5 36.156859627793885 37.0 35.0 37.0 35.0 37.0 6 36.22133171233517 37.0 37.0 37.0 35.0 37.0 7 36.18011939274915 37.0 37.0 37.0 35.0 37.0 8 36.17297505117574 37.0 37.0 37.0 35.0 37.0 9 37.87868699148976 39.0 38.0 39.0 35.0 39.0 10-11 37.83734530378129 39.0 38.0 39.0 35.0 39.0 12-13 37.78237580140694 39.0 38.0 39.0 35.0 39.0 14-15 39.04800310691314 40.0 38.0 41.0 36.0 41.0 16-17 38.97598428878706 40.0 38.0 41.0 36.0 41.0 18-19 38.976863628334215 40.0 38.0 41.0 35.5 41.0 20-21 38.986947587726135 40.0 38.5 41.0 35.5 41.0 22-23 39.01365664972931 40.0 39.0 41.0 35.0 41.0 24-25 38.96275522927772 40.0 38.0 41.0 35.0 41.0 26-27 38.7881480096013 40.0 38.0 41.0 35.0 41.0 28-29 38.706718569780854 40.0 38.0 41.0 34.5 41.0 30-31 38.48991032555046 40.0 38.0 41.0 34.0 41.0 32-33 38.27911354260836 40.0 38.0 41.0 34.0 41.0 34-35 38.06920856843002 40.0 38.0 41.0 33.5 41.0 36-37 37.98704812078515 40.0 37.5 41.0 33.0 41.0 38-39 37.939158535698326 40.0 38.0 41.0 33.0 41.0 40-41 37.81536051622557 40.0 37.0 41.0 33.0 41.0 42-43 37.622617600299264 40.0 37.0 41.0 33.0 41.0 44-45 37.36593799682035 40.0 37.0 41.0 32.5 41.0 46-47 36.99366953458649 40.0 36.5 41.0 31.0 41.0 48-49 36.60602496960628 40.0 36.0 41.0 30.5 41.0 50-51 36.29591944886062 39.0 35.5 41.0 30.0 41.0 52-53 35.83558662468697 39.0 35.0 40.0 29.0 41.0 54-55 35.514840445982315 38.5 35.0 40.0 28.0 41.0 56-57 35.23933024720222 38.0 35.0 40.0 27.0 41.0 58-59 34.858001859991475 38.0 34.0 40.0 26.5 41.0 60-61 34.44734353730894 37.5 34.0 40.0 26.0 41.0 62-63 34.01186056298513 37.0 33.5 40.0 25.0 41.0 64-65 33.57760061099161 36.5 33.0 39.0 23.5 41.0 66-67 33.063518449245095 36.0 32.0 39.0 22.5 40.0 68-69 32.52831499319389 35.0 32.0 38.5 21.5 40.0 70-71 31.92961776655548 35.0 31.0 38.0 19.5 40.0 72-73 31.447775803485143 35.0 31.0 37.0 18.0 39.0 74-75 30.888469091929302 34.5 30.0 36.5 15.0 39.0 76-77 29.688395835281646 33.5 28.5 35.0 13.0 37.5 78-79 30.383633633633636 34.0 30.0 36.0 12.5 38.0 80-81 30.185203715826553 34.0 30.0 35.0 7.5 37.0 82-83 29.710450242630174 34.0 29.5 35.0 6.0 37.0 84-85 29.186165404158483 34.0 29.0 35.0 2.0 36.0 86-87 28.631242921121814 34.0 29.0 35.0 2.0 36.0 88-89 27.983708189158016 33.0 27.0 35.0 2.0 35.5 90-91 27.438048255868324 33.0 26.0 35.0 2.0 35.0 92-93 26.793406641936052 32.5 25.0 35.0 2.0 35.0 94-95 26.025069619792802 32.0 24.5 34.0 2.0 35.0 96-97 25.172092854099773 32.0 20.0 34.0 2.0 35.0 98-99 24.124762305558153 31.5 8.5 34.0 2.0 35.0 100-101 20.62647708261895 27.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.11515484635076945 1101 2 0.09022637054160754 1101 3 0.20410748727603334 1101 4 0.10432154813836547 1101 5 0.054740935399578916 1101 6 0.07888314364321758 1101 7 0.09907402270658139 1101 8 0.15212891849571264 1101 9 0.15540512382101923 1101 10-11 0.17311467163760597 1101 12-13 0.17298198242533402 1101 14-15 0.377481172496438 1101 16-17 0.3571119257030091 1101 18-19 0.3079319022881677 1101 20-21 0.3151760939188506 1101 22-23 0.2370462597504357 1101 24-25 0.27649416458061893 1101 26-27 0.37746934286086997 1101 28-29 0.4016704868792047 1101 30-31 0.3917073912280742 1101 32-33 0.48901505244481314 1101 34-35 0.40412092937488353 1101 36-37 0.47716495702535866 1101 38-39 0.6238680653082724 1101 40-41 0.5307334564221051 1101 42-43 0.6998420432174512 1101 44-45 0.7643309790349164 1101 46-47 0.8490972145601958 1101 48-49 0.7827261925395632 1101 50-51 0.8705648956463321 1101 52-53 0.9663737741007807 1101 54-55 0.9147272026439452 1101 56-57 0.9368799919718143 1101 58-59 0.9334046357106018 1101 60-61 1.0818990184746582 1101 62-63 1.061519459602188 1101 64-65 1.1960444720102927 1101 66-67 1.114484427657949 1101 68-69 1.1742776701166306 1101 70-71 1.2682001548823543 1101 72-73 1.1063266964425473 1101 74-75 1.1517676493466347 1101 76-77 1.2471746561268162 1101 78-79 1.5027809666490732 1101 80-81 1.4709417917429022 1101 82-83 1.5926982793964122 1101 84-85 1.5723575109024779 1101 86-87 1.6345730790732844 1101 88-89 2.0180296420359447 1101 90-91 2.1672562146245404 1101 92-93 2.375082187287031 1101 94-95 2.372738876555143 1101 96-97 2.6025975751586543 1101 98-99 2.9927210119130123 1101 100-101 3.362590849847166 1102 1 0.1410607510016959 1102 2 0.18171349103021583 1102 3 0.30467547145457985 1102 4 0.09707640046370614 1102 5 0.1373131256847273 1102 6 0.08162842099181944 1102 7 0.206701554882315 1102 8 0.16278676487571886 1102 9 0.2563529113130727 1102 10-11 0.26024480896906965 1102 12-13 0.21671129451426197 1102 14-15 0.26038160380058883 1102 16-17 0.274935517511544 1102 18-19 0.2513267662165575 1102 20-21 0.30996701158019846 1102 22-23 0.3342835901141754 1102 24-25 0.11990642079666713 1102 26-27 0.2233527788886036 1102 28-29 0.06896339881522806 1102 30-31 0.26713374810306334 1102 32-33 0.2413473397826138 1102 34-35 0.16304876551618008 1102 36-37 0.14545838642803943 1102 38-39 0.10864397520761315 1102 40-41 0.187956971286674 1102 42-43 0.20135787634490754 1102 44-45 0.1009728417181961 1102 46-47 0.22857695776890807 1102 48-49 0.22848530136612766 1102 50-51 0.40010966019899996 1102 52-53 0.4891327809089674 1102 54-55 0.4684377212291082 1102 56-57 0.7056157263012324 1102 58-59 0.5837852379631201 1102 60-61 0.6708808506015913 1102 62-63 0.7503403977040861 1102 64-65 0.7362790405033408 1102 66-67 0.6877280831603656 1102 68-69 0.4982560422230833 1102 70-71 0.7036566379036806 1102 72-73 0.7060481125557949 1102 74-75 0.7761249643707409 1102 76-77 0.9798905325198888 1102 78-79 1.023496226015407 1102 80-81 1.243868268458744 1102 82-83 1.2881138444491 1102 84-85 1.3374519270562928 1102 86-87 1.2683037923783225 1102 88-89 1.57005378474366 1102 90-91 1.613889239813247 1102 92-93 1.8012580718661546 1102 94-95 1.7730346683590135 1102 96-97 1.954419279012857 1102 98-99 2.1378627252561557 1102 100-101 2.343361924321801 1103 1 0.35880156833145804 1103 2 0.3405628054486698 1103 3 0.4170829663256015 1103 4 0.25919068901668396 1103 5 0.18902850683811323 1103 6 0.1195072369558261 1103 7 0.1713484876157665 1103 8 0.17589040695121128 1103 9 0.1584440962907081 1103 10-11 0.24093245174449862 1103 12-13 0.22297302332307112 1103 14-15 0.37640570536734685 1103 16-17 0.4489033638001132 1103 18-19 0.5439032226649161 1103 20-21 0.5058259774505274 1103 22-23 0.5033282098208716 1103 24-25 0.5458418894559571 1103 26-27 0.5286195191565142 1103 28-29 0.44057135872355957 1103 30-31 0.4683318938854413 1103 32-33 0.4544203599819312 1103 34-35 0.4347230110354161 1103 36-37 0.4009284322273885 1103 38-39 0.5763315935151994 1103 40-41 0.6517838605563355 1103 42-43 0.6827719545485849 1103 44-45 0.7548931833189343 1103 46-47 0.6348063593670261 1103 48-49 0.6026032541639452 1103 50-51 0.5946933324366768 1103 52-53 0.5312041246903121 1103 54-55 0.6293949773903762 1103 56-57 0.48503422947723607 1103 58-59 0.8475385754139708 1103 60-61 0.8843097666983937 1103 62-63 0.671388229371253 1103 64-65 0.6962613272679405 1103 66-67 0.7711675056006371 1103 68-69 0.7301314875563563 1103 70-71 0.9765611339810398 1103 72-73 0.7832884586942193 1103 74-75 0.6246882941920724 1103 76-77 0.5831361177424981 1103 78-79 0.9047888176715766 1103 80-81 1.0340652271800082 1103 82-83 1.0550541184444562 1103 84-85 1.080930134734892 1103 86-87 1.0199676056990619 1103 88-89 1.2046254380802957 1103 90-91 1.3228870666239239 1103 92-93 1.5798233517004263 1103 94-95 1.5775328959233015 1103 96-97 1.7194670024283631 1103 98-99 2.118792636749294 1103 100-101 2.3730162085298865 1104 1 0.0889512775358341 1104 2 0.1311552336230477 1104 3 0.15077702650359015 1104 4 0.07632775834142791 1104 5 0.0730774490681867 1104 6 0.10865689527612687 1104 7 0.11354967775305624 1104 8 0.11122912299468624 1104 9 0.08069460272479745 1104 10-11 0.09585902739743091 1104 12-13 0.08999746005697773 1104 14-15 0.22628444311218487 1104 16-17 0.1849489215514737 1104 18-19 0.12793604014373017 1104 20-21 0.12742602723440655 1104 22-23 0.19517355523436208 1104 24-25 0.09504919805962686 1104 26-27 0.21187817666955056 1104 28-29 0.13812328454832823 1104 30-31 0.2709965691968961 1104 32-33 0.1169645569280604 1104 34-35 0.19659550379130764 1104 36-37 0.22680845170086883 1104 38-39 0.27719442242230485 1104 40-41 0.2796588108755955 1104 42-43 0.25317677863407795 1104 44-45 0.26078473422433035 1104 46-47 0.36893241847781866 1104 48-49 0.39676856965210305 1104 50-51 0.38892534732233486 1104 52-53 0.574838595489048 1104 54-55 0.552528907030073 1104 56-57 0.7282867602724181 1104 58-59 0.6339170800547365 1104 60-61 0.6865491473979262 1104 62-63 0.6767912243804872 1104 64-65 0.6747165623454947 1104 66-67 0.7254242680256411 1104 68-69 0.7060485355097867 1104 70-71 0.6155346577583352 1104 72-73 0.6945151706339594 1104 74-75 0.6390027069004418 1104 76-77 0.6813451357760911 1104 78-79 0.8255200218501209 1104 80-81 0.8172443955141624 1104 82-83 0.95784094992052 1104 84-85 1.154406741124557 1104 86-87 1.0347054220570513 1104 88-89 1.1453105921696576 1104 90-91 1.2144482863564932 1104 92-93 1.549326943153556 1104 94-95 1.5942911195464369 1104 96-97 1.9147688550213182 1104 98-99 2.1172482362668603 1104 100-101 2.206666364001368 1105 1 0.11145072524672628 1105 2 0.08543228210896103 1105 3 0.1705195888413229 1105 4 0.10194383937896134 1105 5 0.05067883212172575 1105 6 0.07322172185735099 1105 7 0.039335195009812196 1105 8 0.08440509953304343 1105 9 0.058842194642927836 1105 10-11 0.07648710678924431 1105 12-13 0.03732275935713858 1105 14-15 0.070797070772052 1105 16-17 0.21902389302498193 1105 18-19 0.16138322610403577 1105 20-21 0.06530895612996801 1105 22-23 0.1383105776562843 1105 24-25 0.10525438613180427 1105 26-27 0.12625259017904256 1105 28-29 0.08262025557189645 1105 30-31 0.06621790032512109 1105 32-33 0.0942601542211321 1105 34-35 0.1532215544073452 1105 36-37 0.21709753704929113 1105 38-39 0.14884091280234202 1105 40-41 0.20926601677911094 1105 42-43 0.23175328131647888 1105 44-45 0.22681135354614668 1105 46-47 0.37918346174688367 1105 48-49 0.40319133734004 1105 50-51 0.4321878255094447 1105 52-53 0.5847534517621398 1105 54-55 0.5908549846018687 1105 56-57 0.5590554960471721 1105 58-59 0.7289708497066059 1105 60-61 0.7587206671151563 1105 62-63 0.7638669498046298 1105 64-65 0.8792147798197263 1105 66-67 0.9317176784565007 1105 68-69 0.9720000637798947 1105 70-71 1.0381120846383034 1105 72-73 0.9232345591232409 1105 74-75 1.1159117018153246 1105 76-77 1.1972449819458362 1105 78-79 1.1314567751197728 1105 80-81 1.310284032645015 1105 82-83 1.3327968681290336 1105 84-85 1.3746717993631918 1105 86-87 1.6134532357015345 1105 88-89 1.7358512984106262 1105 90-91 1.7471144139079122 1105 92-93 2.0699435410120905 1105 94-95 1.9763214052572486 1105 96-97 2.259778542881701 1105 98-99 2.84589998200714 1105 100-101 2.843973290821541 1106 1 0.004555000093084516 1106 2 -0.015346983915939916 1106 3 0.05121203484431902 1106 4 -0.01603317569767171 1106 5 -2.2780533694088945E-4 1106 6 -0.03784378621645601 1106 7 -0.054406755874886414 1106 8 -0.05801963767187601 1106 9 -0.13700310948769356 1106 10-11 0.017618327902376052 1106 12-13 0.0036647401181753025 1106 14-15 -0.05142416990958765 1106 16-17 -0.024330360357076586 1106 18-19 -0.010417835250137841 1106 20-21 -0.03182447940490363 1106 22-23 -0.024787265342105513 1106 24-25 -0.05012536337352458 1106 26-27 -0.12288598388621352 1106 28-29 -0.22511277608658986 1106 30-31 -0.12905493955907588 1106 32-33 -0.1454655145595396 1106 34-35 -0.25988497357095497 1106 36-37 -0.12404413085395305 1106 38-39 -0.2105873524895543 1106 40-41 -0.22192549298305408 1106 42-43 -0.3557490077960779 1106 44-45 -0.19848392336327692 1106 46-47 -0.40643909994501115 1106 48-49 -0.07461667795346472 1106 50-51 -0.13397633649173457 1106 52-53 -0.22971230038441348 1106 54-55 0.03613353175857981 1106 56-57 0.06921059489960868 1106 58-59 0.05357122896726452 1106 60-61 -0.0017427485236680695 1106 62-63 -0.01263427378496118 1106 64-65 -0.01229158279654996 1106 66-67 0.04714317051024608 1106 68-69 -0.27899114056874197 1106 70-71 -0.10761691520437466 1106 72-73 0.09491241783399929 1106 74-75 0.21793155518308893 1106 76-77 0.43899387882932217 1106 78-79 0.5235678604792291 1106 80-81 0.5657713270157103 1106 82-83 0.5042460637261961 1106 84-85 0.571340935872712 1106 86-87 0.8664525314737332 1106 88-89 1.0742132764619434 1106 90-91 0.9274349442574525 1106 92-93 0.8592173923242434 1106 94-95 1.3202907785441376 1106 96-97 1.1340204061721728 1106 98-99 1.6790198302631687 1106 100-101 1.937843779408734 1107 1 0.05121886562318423 1107 2 0.07259095747890854 1107 3 0.12591100859229698 1107 4 0.010788186613993389 1107 5 0.01932595058429598 1107 6 -8.558330222996346E-4 1107 7 0.017787742769627357 1107 8 0.009887539185363892 1107 9 0.014626154331402574 1107 10-11 -0.061107159754392626 1107 12-13 -0.02020427700905003 1107 14-15 0.12072653699367919 1107 16-17 0.032686798523009486 1107 18-19 -0.05311754093893484 1107 20-21 -1.037661511134047E-4 1107 22-23 0.03894338810933817 1107 24-25 3.9443223343482714E-5 1107 26-27 0.054700438851334354 1107 28-29 0.09601882553039331 1107 30-31 0.15044500959837137 1107 32-33 0.1509845484660346 1107 34-35 0.15501551413378678 1107 36-37 0.2468866666833449 1107 38-39 0.20827297823642255 1107 40-41 0.35819552666902865 1107 42-43 0.34508672856708245 1107 44-45 0.2951617175118244 1107 46-47 0.30325475117650313 1107 48-49 0.32414058149893066 1107 50-51 0.34682676471179263 1107 52-53 0.15034749236985334 1107 54-55 0.3650068705023273 1107 56-57 0.3827628197886739 1107 58-59 0.38825093051192283 1107 60-61 0.429131652157821 1107 62-63 0.3663141776277925 1107 64-65 0.3507796044440781 1107 66-67 0.32842152961978854 1107 68-69 0.463665828601485 1107 70-71 0.5304824752606301 1107 72-73 0.3972277202895995 1107 74-75 0.47465428295290835 1107 76-77 0.6830008135609198 1107 78-79 0.6934844930909811 1107 80-81 0.7321261034332096 1107 82-83 0.8636333246942804 1107 84-85 0.9518616690798041 1107 86-87 1.0935943697100186 1107 88-89 1.1972036768134267 1107 90-91 1.2372164268261336 1107 92-93 1.3378556082688497 1107 94-95 1.543839223817411 1107 96-97 1.4695310955779313 1107 98-99 1.7211548719060943 1107 100-101 2.0783932050798057 1108 1 0.059628364050830385 1108 2 0.054822281361772696 1108 3 0.10845911931644991 1108 4 0.048016431192515086 1108 5 -0.026990329398046242 1108 6 0.009269083182473992 1108 7 -0.026472488423451068 1108 8 0.018890416836157442 1108 9 0.002787182873561278 1108 10-11 0.030144822718092712 1108 12-13 -0.015262061653949388 1108 14-15 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-4.049707171670342 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 8.0 4 17.0 5 27.0 6 72.0 7 196.0 8 445.0 9 815.0 10 1512.0 11 2360.0 12 3758.0 13 5582.0 14 7434.0 15 10018.0 16 13245.0 17 18964.0 18 19130.0 19 14006.0 20 12442.0 21 12509.0 22 13275.0 23 14626.0 24 16244.0 25 19298.0 26 22365.0 27 27002.0 28 32244.0 29 39370.0 30 48641.0 31 59310.0 32 74842.0 33 97407.0 34 134828.0 35 199762.0 36 301426.0 37 416713.0 38 267804.0 39 17042.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.85501418373391 18.683666365327266 14.217296881656743 23.244022569282087 2 15.823259033095805 19.923316501553103 40.24657397582999 24.0068504895211 3 19.929410102055556 23.73994486235853 29.606710456719654 26.72393457886626 4 12.68655126747055 16.37972732926386 36.47860826844575 34.45511313481984 5 13.210573321674563 37.86258962940796 34.39997589273514 14.526861156182344 6 32.89166260117616 35.91940093633571 16.809984948592977 14.378951513895144 7 29.039610544800855 32.86880305911448 19.85561686253728 18.23596953354739 8 26.702297508698592 35.31178545432441 19.031327396937865 18.95458964003913 9 26.469237403493455 16.369431715452475 19.893803838440515 37.267527042613544 10-11 25.28136267755645 26.571536934858734 26.856718310005505 21.29038207757931 12-13 26.01465652503715 23.897305610108376 27.49958435944595 22.588453505408523 14-15 22.466437025260554 25.466998140008524 26.032632978999757 26.03393185573116 16-17 21.825805043798123 28.70670843854235 27.047653189521704 22.419833328137827 18-19 21.74605401249 26.91114124505128 29.671072456539584 21.671732285919138 20-21 22.3215603146399 25.99545912694702 30.14360381142388 21.539376746989202 22-23 21.93569001527479 26.181821960368673 29.919391710049148 21.963096314307386 24-25 21.740808841675832 26.241685080763112 30.237963987171007 21.77954209039005 26-27 21.364849310014215 26.75177920454811 29.840488693379204 22.042882792058474 28-29 21.423765569683916 27.056673805315434 29.80949575369895 21.710064871301697 30-31 21.641066192697025 26.544854321617528 29.80239113250047 22.01168835318497 32-33 21.219114153713782 26.61783746599414 29.79698316800612 22.366065212285964 34-35 22.005915523989465 26.810226207475672 29.618904942143846 21.564953326391013 36-37 22.06657197926715 26.28827558865487 29.60284550617187 22.042306925906114 38-39 21.537273511961832 26.722587457758003 29.664807836913543 22.075331193366623 40-41 22.073507249389387 26.440445876422597 29.690614769006913 21.795432105181106 42-43 21.67439598188199 26.46483925891071 29.589424353305876 22.271340405901423 44-45 21.71839829404099 26.8219862533956 29.645298532434627 21.81431692012878 46-47 22.124238418097004 27.026159216436685 28.856241860721273 21.993360504745038 48-49 21.927136330444014 27.06535950598824 28.87384552079082 22.133658642776926 50-51 22.135717031910804 27.412611573511224 28.612176190030862 21.839495204547106 52-53 22.33356193563806 27.110466868252338 28.661013955131605 21.894957240978002 54-55 21.862355435019794 27.312520132589334 28.781134075251725 22.043990357139144 56-57 21.92459760798861 27.539459875100015 28.35938880056527 22.176553716346103 58-59 21.9179213815892 27.1058948221578 28.487587933954718 22.488595862298286 60-61 21.678928063011107 27.41100096636429 28.733413344139986 22.176657626484616 62-63 21.8219863462078 27.306960940178932 28.88582353980278 21.985229173810488 64-65 21.489551835572595 27.325742697715015 28.732348265220235 22.45235720149215 66-67 21.442187396089647 27.508681710115052 28.53918667287358 22.509944220921728 68-69 22.024833009609367 27.13644179346108 28.616930161609133 22.22179503532042 70-71 21.8268961002525 27.242562631835987 28.31847418352609 22.612067084385423 72-73 21.722102725562934 27.402480335006285 28.30026393175182 22.575153007678956 74-75 21.71080249799973 27.747410039797582 28.0678169519 22.473970510302692 76-77 21.753275767116598 27.397960243981007 28.335073828153412 22.513690160748983 78-79 21.88607292413521 27.58364766150233 28.211914336481808 22.318365077880646 80-81 21.925878704467156 27.59291269404599 27.975900084011478 22.505308517475374 82-83 21.880379847951712 27.6731780118381 28.10833278130427 22.338109358905918 84-85 21.81773829261361 27.72485648121929 28.039145594990494 22.418259631176607 86-87 21.9033685080644 27.69887326731696 27.855882751011897 22.541875473606744 88-89 21.940538935548908 27.7678500222238 27.67152524875457 22.620085793472725 90-91 21.859423437774804 27.7348049649121 27.801050207919136 22.604721389393955 92-93 21.880664758519252 27.499741522053235 27.953181980424258 22.666411739003255 94-95 21.71333360003616 27.339992591203277 28.0642466329201 22.882427175840466 96-97 21.95470748410299 27.019747674092976 27.979309337970516 23.04623550383352 98-99 21.686182365601454 27.232199799192873 27.791056679304788 23.290561155900885 100-101 21.87759782364726 26.90786766478515 27.980483070502853 23.234051441064736 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1338.0 1 873.5 2 583.0 3 761.5 4 610.0 5 561.0 6 1138.0 7 2376.0 8 3635.5 9 4044.0 10 3264.0 11 2375.0 12 2188.5 13 2287.5 14 2407.5 15 2511.0 16 2677.0 17 2844.0 18 3058.0 19 3368.5 20 3874.0 21 4564.5 22 5347.0 23 6498.0 24 8017.5 25 9927.5 26 12684.0 27 15599.0 28 17818.5 29 20042.0 30 22939.0 31 27454.0 32 33820.5 33 37101.5 34 38364.5 35 41445.5 36 45655.5 37 49816.0 38 52258.5 39 53943.0 40 56227.0 41 58801.0 42 60155.5 43 61883.0 44 64192.0 45 67470.5 46 69391.5 47 70363.0 48 71512.5 49 70399.5 50 69222.5 51 67603.0 52 65522.5 53 64823.5 54 64529.0 55 60406.0 56 54798.0 57 50143.0 58 45763.0 59 40257.0 60 32626.0 61 26581.0 62 22011.0 63 16167.5 64 11151.0 65 8396.5 66 6158.0 67 4810.5 68 3847.0 69 2964.0 70 2257.5 71 1458.5 72 1002.0 73 786.0 74 576.0 75 425.0 76 270.0 77 150.5 78 100.0 79 62.5 80 31.0 81 16.5 82 12.0 83 6.0 84 4.0 85 2.0 86 0.5 87 1.0 88 1.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.010702744266758107 3 0.005299417064122946 4 5.195506925610732E-5 5 4.6759562330496587E-4 6 1.5586520776832196E-4 7 0.0 8 1.5586520776832196E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0019223375624759708 26-27 0.02208090443384561 28-29 3.117304155366439E-4 30-31 1.8184274239637561E-4 32-33 0.003740764986439727 34-35 0.016209981607905485 36-37 0.008390743684861332 38-39 0.021353533464260107 40-41 0.02286023047268722 42-43 0.01852198218980226 44-45 0.009975373297172605 46-47 0.002363955651152883 48-49 4.416180886769122E-4 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 2.0782027702442927E-4 68-69 1.8184274239637561E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 1.5586520776832196E-4 82-83 0.0011689890582624147 84-85 0.0041044504712324775 86-87 8.053035734696634E-4 88-89 7.793260388416098E-5 90-91 0.003818697590323888 92-93 5.715057618171806E-4 94-95 5.195506925610732E-5 96-97 3.6368548479275123E-4 98-99 3.896630194208049E-4 100-101 0.0027016636013175803 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1924740.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.53913187954052 #Duplication Level Percentage of deduplicated Percentage of total 1 80.17894370784465 47.737847033836175 2 11.863754937298816 14.127153395967685 3 3.5159369836797674 6.280075072543907 4 1.452890937890421 3.4601546063058826 5 0.7774890615397698 2.3145511884958276 6 0.47394114616300337 1.6930826642723786 7 0.3027364983009438 1.261726780796313 8 0.222275109212123 1.0587253632735891 9 0.1676819198867341 0.8985272347755726 >10 0.9334686622751298 10.315431724894772 >50 0.0698206316290301 2.8354850254622694 >100 0.03599464898892919 3.886993762943386 >500 0.003406283729966924 1.4883446642371532 >1k 0.0013974497353710451 1.428490546332923 >5k 2.6202182538207094E-4 1.2134109358621197 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9765 0.5073412512858879 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8205 0.4262913432463606 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5364 0.2786869914897597 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 2516 0.130718954248366 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 2478 0.12874466161663392 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 2216 0.11513243347153382 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 2114 0.10983301640741087 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2079 0.10801458898344711 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 5.195506925610732E-5 2 0.0 0.0 0.0 0.0 5.195506925610732E-5 3 0.0 0.0 0.0 0.0 5.195506925610732E-5 4 0.0 0.0 0.0 0.0 5.195506925610732E-5 5 0.0 0.0 0.0 0.0 5.195506925610732E-5 6 0.0 0.0 0.0 0.0 5.195506925610732E-5 7 0.0 0.0 0.0 0.0 5.195506925610732E-5 8 5.195506925610732E-5 0.0 0.0 0.0 5.195506925610732E-5 9 5.195506925610732E-5 0.0 0.0 0.0 5.195506925610732E-5 10-11 5.195506925610732E-5 0.0 0.0 0.0 1.8184274239637561E-4 12-13 5.195506925610732E-5 0.0 0.0 0.0 3.6368548479275123E-4 14-15 1.0391013851221464E-4 0.0 0.0 5.195506925610732E-5 4.935731579330195E-4 16-17 1.0391013851221464E-4 0.0 0.0 5.195506925610732E-5 5.195506925610732E-4 18-19 1.0391013851221464E-4 0.0 0.0 5.195506925610732E-5 6.754159003293952E-4 20-21 1.0391013851221464E-4 0.0 0.0 7.793260388416098E-5 7.273709695855025E-4 22-23 1.0391013851221464E-4 0.0 0.0 2.3379781165248293E-4 7.273709695855025E-4 24-25 1.0391013851221464E-4 0.0 0.0 4.4161808867691223E-4 7.273709695855025E-4 26-27 1.0391013851221464E-4 0.0 0.0 0.0014027868699148975 8.312811080977171E-4 28-29 1.0391013851221464E-4 0.0 0.0 0.004494113490653283 8.312811080977171E-4 30-31 1.0391013851221464E-4 0.0 0.0 0.015352722965179713 8.312811080977171E-4 32-33 1.298876731402683E-4 0.0 0.0 0.03764144767604975 8.312811080977171E-4 34-35 2.0782027702442927E-4 0.0 0.0 0.0713083325540073 8.312811080977171E-4 36-37 2.597753462805366E-4 0.0 0.0 0.12791338050853623 8.312811080977171E-4 38-39 3.117304155366439E-4 0.0 0.0 0.21582135768986982 8.832361773538244E-4 40-41 3.117304155366439E-4 0.0 0.0 0.3029759863669898 9.351912466099317E-4 42-43 3.377079501646976E-4 0.0 0.0 0.389221401332128 9.351912466099317E-4 44-45 3.6368548479275123E-4 0.0 0.0 0.4963787316728493 9.87146315866039E-4 46-47 4.6759562330496587E-4 0.0 0.0 0.6188887849787503 9.87146315866039E-4 48-49 5.195506925610732E-4 0.0 0.0 0.7481529972879454 0.0010131238504940926 50-51 5.195506925610732E-4 0.0 0.0 0.8903540218419111 0.0010391013851221464 52-53 5.195506925610732E-4 0.0 0.0 1.0192545486663134 0.0010391013851221464 54-55 5.195506925610732E-4 0.0 0.0 1.1566497293140892 0.0010910564543782537 56-57 5.715057618171804E-4 0.0 0.0 1.3152166006837287 0.0010910564543782537 58-59 5.715057618171804E-4 0.0 0.0 1.4825379012230224 0.0011170339890063073 60-61 5.715057618171804E-4 0.0 0.0 1.6668744869436911 0.001143011523634361 62-63 5.715057618171804E-4 0.0 0.0 1.8443010484532976 0.001143011523634361 64-65 5.715057618171804E-4 0.0 0.0 2.0196753847272877 0.001143011523634361 66-67 6.754159003293951E-4 0.0 0.0 2.2190789405322278 0.001143011523634361 68-69 7.273709695855025E-4 0.0 0.0 2.445213379469435 0.0011949665928904683 70-71 7.273709695855025E-4 0.0 0.0 2.6799204048339 0.0012728991967746292 72-73 7.273709695855025E-4 0.0 0.0 2.912497272358864 0.001298876731402683 74-75 7.273709695855025E-4 0.0 0.0 3.1383199808805347 0.001298876731402683 76-77 7.273709695855025E-4 0.0 0.0 3.3802227833369702 0.001298876731402683 78-79 7.273709695855025E-4 0.0 0.0 3.647921277679063 0.001298876731402683 80-81 7.793260388416097E-4 0.0 0.0 3.9318557311636897 0.001298876731402683 82-83 7.793260388416097E-4 0.0 0.0 4.228545154150691 0.001376809335286844 84-85 7.793260388416097E-4 0.0 0.0 4.526273678522814 0.0014027868699148975 86-87 7.793260388416097E-4 0.0 0.0 4.832730654529962 0.0014027868699148975 88-89 7.793260388416097E-4 5.195506925610732E-5 0.0 5.15976183796253 0.0014027868699148975 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5115 0.0 46.531223 1 GTATCAA 8235 0.0 36.343716 1 ATCAACG 10075 0.0 29.37237 3 TCAACGC 10290 0.0 29.034876 4 CAACGCA 10400 0.0 28.727774 5 TATCAAC 10320 0.0 28.724817 2 AACGCAG 10765 0.0 27.753006 6 TACATGG 7800 0.0 24.666801 2 ACGCAGA 12165 0.0 24.520027 7 GTACATG 8100 0.0 24.515663 1 CGCAGAG 12255 0.0 24.301197 8 ACATGGG 7830 0.0 23.598467 3 GAGTACT 8170 0.0 22.324543 12-13 GCAGAGT 13350 0.0 21.987741 9 GTACTTT 8725 0.0 20.768377 14-15 TAAGGTG 985 0.0 20.735617 5 TAGCCCT 1425 0.0 20.666243 4 CATGGGG 3985 0.0 20.501463 4 GTAAGGT 885 0.0 19.858349 4 TGGTATC 2705 0.0 19.84557 2 >>END_MODULE