FastQCFastQC Report
Thu 26 May 2016
SRR937937_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937937_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1948314
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101910.5230676369414786No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84990.4362233192390959No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54510.27978036394544203No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA24850.12754617582176178No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA24460.1255444450945792No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA22550.11574109717427479No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20850.10701560426091483No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA20090.1031147956643539No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA51050.050.637671
GTATCAA81650.035.9907651
ATCAACG103750.028.1198673
TATCAAC105250.027.80942
TCAACGC104650.027.7872264
CAACGCA106800.027.1833485
CGCGACG350.0084008327.13832590-91
GTACATG85450.026.7852441
AACGCAG108750.026.6959256
TACATGG85600.026.2555052
CGCGCGT1109.424636E-525.9313987
ACATGGG84200.025.6762923
GTATAGG5250.025.4841961
ACGCAGA121650.023.7606777
CGCAGAG122250.023.682958
TACCGTA1452.3199356E-522.9507777
GAGTACT81050.022.14131412-13
GCAGAGT131950.021.2213619
GTACTTT84350.021.06213414-15
GTACCGT1856.537768E-620.5471326
Produced by FastQC (version 0.11.5)