##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937937_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1948314 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.98265987925971 33.0 31.0 34.0 30.0 34.0 2 32.16686324688936 34.0 31.0 34.0 30.0 34.0 3 32.24099144183125 34.0 31.0 34.0 30.0 34.0 4 35.7495911849938 37.0 35.0 37.0 35.0 37.0 5 35.77684449221224 37.0 35.0 37.0 35.0 37.0 6 35.805913728485244 37.0 36.0 37.0 35.0 37.0 7 35.75140352119833 37.0 36.0 37.0 35.0 37.0 8 35.73504732810009 37.0 36.0 37.0 35.0 37.0 9 37.4200334237705 39.0 38.0 39.0 35.0 39.0 10-11 37.34127609820594 39.0 37.0 39.0 34.5 39.0 12-13 37.25096955624196 39.0 37.0 39.0 34.0 39.0 14-15 38.52850695524438 40.0 38.0 41.0 34.0 41.0 16-17 38.45301604361514 40.0 38.0 41.0 34.0 41.0 18-19 38.40892843761324 40.0 38.0 41.0 34.0 41.0 20-21 38.309625142559156 40.0 38.0 41.0 34.0 41.0 22-23 38.16375158213717 40.0 38.0 41.0 34.0 41.0 24-25 38.12175706790589 40.0 38.0 41.0 33.5 41.0 26-27 38.13445137693411 40.0 38.0 41.0 34.0 41.0 28-29 38.0488776449792 40.0 38.0 41.0 33.0 41.0 30-31 37.864474874173254 40.0 38.0 41.0 33.0 41.0 32-33 37.64123596093853 40.0 37.0 41.0 33.0 41.0 34-35 37.44162568251319 40.0 37.0 41.0 32.0 41.0 36-37 37.23384244018162 40.0 37.0 41.0 31.5 41.0 38-39 37.000837647319685 40.0 36.0 41.0 31.0 41.0 40-41 36.78570138078359 40.0 36.0 41.0 30.5 41.0 42-43 36.50031155142344 39.0 35.5 41.0 30.0 41.0 44-45 36.273787233474685 39.0 35.0 41.0 30.0 41.0 46-47 36.120688195024 39.0 35.0 41.0 29.5 41.0 48-49 35.85561105653401 39.0 35.0 41.0 28.0 41.0 50-51 35.20499852693149 38.5 34.5 40.5 26.5 40.5 52-53 35.27768034310691 39.0 35.0 40.0 27.0 41.0 54-55 35.419658227575226 39.0 35.0 41.0 27.0 41.0 56-57 35.22298074129735 39.0 35.0 41.0 26.0 41.0 58-59 34.97261555375571 38.5 35.0 40.0 26.0 41.0 60-61 34.61955542073814 38.0 34.5 40.0 25.5 41.0 62-63 34.21707999839862 37.5 34.0 40.0 24.0 41.0 64-65 33.805183096769824 37.0 34.0 40.0 22.5 41.0 66-67 33.387822496784395 36.5 33.0 39.5 22.0 41.0 68-69 32.914694961900395 36.0 33.0 39.0 20.5 41.0 70-71 32.441332351972015 35.0 32.0 39.0 18.5 40.0 72-73 31.957302826957047 35.0 32.0 38.0 16.0 40.0 74-75 31.440700010367937 35.0 31.0 37.0 10.0 39.0 76-77 30.918156159633405 35.0 31.0 36.5 7.5 39.0 78-79 30.362088965125743 34.5 30.0 36.0 4.0 38.5 80-81 29.844490672448075 34.0 30.0 35.5 2.0 37.5 82-83 29.312487360866882 34.0 29.0 35.0 2.0 37.0 84-85 28.831616207654413 34.0 29.0 35.0 2.0 36.5 86-87 28.347773510840653 34.0 28.5 35.0 2.0 36.0 88-89 27.813379670833346 34.0 27.0 35.0 2.0 36.0 90-91 27.23424278632705 33.0 26.0 35.0 2.0 35.0 92-93 26.650422878447724 33.0 25.0 35.0 2.0 35.0 94-95 26.066944804584885 32.5 24.5 35.0 2.0 35.0 96-97 25.261232532333082 32.0 19.0 34.5 2.0 35.0 98-99 24.231025389131318 32.0 4.5 34.0 2.0 35.0 100-101 21.34069713608792 28.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.42227809596355925 1101 2 0.32058639880954587 1101 3 0.2843574487407352 1101 4 0.2056931174803509 1101 5 0.29989933046697814 1101 6 0.2304033651648183 1101 7 0.32031277531166324 1101 8 0.2914487193172661 1101 9 0.27131230834980613 1101 10-11 0.3020197552359747 1101 12-13 0.23950163295117477 1101 14-15 0.2517423283276443 1101 16-17 0.4042482601253994 1101 18-19 0.4057542131127505 1101 20-21 0.36204228104783454 1101 22-23 0.34048765201210074 1101 24-25 0.3899578687005558 1101 26-27 0.29290466321003805 1101 28-29 0.31757366465010506 1101 30-31 0.4863529133237492 1101 32-33 0.486868260437177 1101 34-35 0.403676811138169 1101 36-37 0.4081137275760369 1101 38-39 0.47280594270386445 1101 40-41 0.5911196034088135 1101 42-43 0.4617376266322353 1101 44-45 0.4592673698626868 1101 46-47 0.46916385007988737 1101 48-49 0.6011729756326716 1101 50-51 0.6904526101622537 1101 52-53 0.6788924457951069 1101 54-55 0.631701391925354 1101 56-57 0.5526977912598312 1101 58-59 0.49064945099546975 1101 60-61 0.5787492247209656 1101 62-63 0.529085030653107 1101 64-65 0.6621847995317651 1101 66-67 0.6001875746619945 1101 68-69 0.5056653164224585 1101 70-71 0.6290284884772177 1101 72-73 0.7438615781154425 1101 74-75 0.7421383951758251 1101 76-77 0.7455404113148205 1101 78-79 0.8843304903612754 1101 80-81 0.9827134370657014 1101 82-83 0.9952330287046074 1101 84-85 0.868102875046219 1101 86-87 0.9085522636092698 1101 88-89 0.8256355822980268 1101 90-91 0.7072103635466931 1101 92-93 0.6364084149667804 1101 94-95 0.8315429619758739 1101 96-97 0.9442721423881153 1101 98-99 0.9106616663968694 1101 100-101 0.6803952304405207 1102 1 0.3958219338797342 1102 2 0.2343562061054172 1102 3 0.2667736159901253 1102 4 0.22166450601184806 1102 5 0.18556884456943834 1102 6 0.34378444007980846 1102 7 0.23273260150010344 1102 8 0.2929732772552569 1102 9 0.3165019992292386 1102 10-11 0.40115159422352775 1102 12-13 0.23268745683424186 1102 14-15 0.3613105137330237 1102 16-17 0.33510597044264756 1102 18-19 0.32742661132011364 1102 20-21 0.36606233107725217 1102 22-23 0.4225827055091358 1102 24-25 0.37293872025285424 1102 26-27 0.23272796993434497 1102 28-29 0.32475283787336906 1102 30-31 0.2758162450149442 1102 32-33 0.3865012305869371 1102 34-35 0.4011266143207308 1102 36-37 0.34561163504552894 1102 38-39 0.5189716495965371 1102 40-41 0.5573670863935547 1102 42-43 0.3977067911027419 1102 44-45 0.34926931890537816 1102 46-47 0.4316773618482799 1102 48-49 0.3624364694650879 1102 50-51 0.3705752444070569 1102 52-53 0.45483294857637446 1102 54-55 0.5563777671363965 1102 56-57 0.5257824681970362 1102 58-59 0.3376044603939192 1102 60-61 0.45684108800578116 1102 62-63 0.5366220265924042 1102 64-65 0.499811009014401 1102 66-67 0.504881778232928 1102 68-69 0.3636270362673031 1102 70-71 0.3846750325734192 1102 72-73 0.5244965236139976 1102 74-75 0.4330085650175626 1102 76-77 0.22876070814326255 1102 78-79 0.148983285742883 1102 80-81 0.39420059251560957 1102 82-83 0.4799808728003221 1102 84-85 0.46016495026252713 1102 86-87 0.4772309083191111 1102 88-89 0.369588886583049 1102 90-91 0.7278170060755222 1102 92-93 0.6825959439632747 1102 94-95 0.6467055575746237 1102 96-97 0.3623863419413915 1102 98-99 0.4883277296175983 1102 100-101 0.5536871276841175 1103 1 0.319347784099012 1103 2 0.2045178810163506 1103 3 0.2519725069881318 1103 4 0.14403202889786826 1103 5 0.08995599473354332 1103 6 0.17901091586597317 1103 7 0.21853111098646139 1103 8 0.2483817658893841 1103 9 0.3336852183272683 1103 10-11 0.25639165201132386 1103 12-13 0.1608694089021654 1103 14-15 0.22743954976155578 1103 16-17 0.22668898662883663 1103 18-19 0.315029820506318 1103 20-21 0.2809679349970935 1103 22-23 0.27747210921017995 1103 24-25 0.38339814723732246 1103 26-27 0.29573875605405675 1103 28-29 0.2731762970993117 1103 30-31 0.4087714173964869 1103 32-33 0.43220723752521195 1103 34-35 0.33852492463557127 1103 36-37 0.3486775206107353 1103 38-39 0.358260452977774 1103 40-41 0.340668705012817 1103 42-43 0.3551362161235474 1103 44-45 0.2062614782800054 1103 46-47 0.2554450465027216 1103 48-49 0.33374419881624107 1103 50-51 0.2155557480398329 1103 52-53 0.4006060473299229 1103 54-55 0.34184584358834513 1103 56-57 0.26781443849896647 1103 58-59 0.33585878746534803 1103 60-61 0.3373948643165292 1103 62-63 0.21064522119139184 1103 64-65 0.27418614343179826 1103 66-67 0.3332167323673758 1103 68-69 0.4842526265350173 1103 70-71 0.5623321723581611 1103 72-73 0.4161648656279624 1103 74-75 0.509118618971538 1103 76-77 0.4405709715546102 1103 78-79 0.4589597451651919 1103 80-81 0.502473223730366 1103 82-83 0.5548724038028219 1103 84-85 0.536520462077231 1103 86-87 0.49165447975436294 1103 88-89 0.5023792515438252 1103 90-91 0.6125955797082057 1103 92-93 0.5533296525744049 1103 94-95 0.7256249351869961 1103 96-97 0.753103242533296 1103 98-99 0.8680972096209629 1103 100-101 0.8383691545582472 1104 1 0.24847724653401926 1104 2 0.1455664240402328 1104 3 0.08071714952350106 1104 4 0.15344856410375485 1104 5 0.16237283173727945 1104 6 0.18275823920644285 1104 7 0.1301902916660964 1104 8 0.12426767524611648 1104 9 0.06535928991194595 1104 10-11 0.13321556678272373 1104 12-13 0.015858857032178264 1104 14-15 0.07994828586973313 1104 16-17 0.18400020543892026 1104 18-19 0.2220014614575163 1104 20-21 0.22703842745822556 1104 22-23 0.24555505166185299 1104 24-25 0.3552067178541449 1104 26-27 0.3237469510265214 1104 28-29 0.40743764961799656 1104 30-31 0.4253003772772743 1104 32-33 0.500993518214905 1104 34-35 0.5478785300144295 1104 36-37 0.5616651369493866 1104 38-39 0.5055360087888232 1104 40-41 0.514356409458756 1104 42-43 0.5993818817867975 1104 44-45 0.47111355311260894 1104 46-47 0.41212096977752566 1104 48-49 0.5841323628129729 1104 50-51 0.5778900494109678 1104 52-53 0.5761178047330944 1104 54-55 0.5190951496191047 1104 56-57 0.4685655613970212 1104 58-59 0.5217538281417333 1104 60-61 0.6578867727070659 1104 62-63 0.6022362265098948 1104 64-65 0.625312053938238 1104 66-67 0.6420801649697268 1104 68-69 0.6031297505894102 1104 70-71 0.6578734407272009 1104 72-73 0.6473358026052765 1104 74-75 0.6969508867999998 1104 76-77 0.7623459353775601 1104 78-79 0.6426487872499642 1104 80-81 0.6157143005185759 1104 82-83 0.7433352135739959 1104 84-85 0.5718549933895858 1104 86-87 0.49781742959069675 1104 88-89 0.5475846730714089 1104 90-91 0.48529520019463845 1104 92-93 0.5342595934640357 1104 94-95 0.5457869940010944 1104 96-97 0.5648072230590451 1104 98-99 0.825740765352414 1104 100-101 1.1046900065789096 1105 1 0.29837623130546476 1105 2 0.3024130742622191 1105 3 0.29462975022146054 1105 4 0.20587548080916918 1105 5 0.15640742523373063 1105 6 0.14170400707263298 1105 7 0.10847875536327933 1105 8 0.2331254848968456 1105 9 0.2970569030951893 1105 10-11 0.29729773856958275 1105 12-13 0.1814187739382902 1105 14-15 0.21859911177160996 1105 16-17 0.3562844840886612 1105 18-19 0.3198920342679017 1105 20-21 0.28889779322232556 1105 22-23 0.2720661523471293 1105 24-25 0.32589934922431496 1105 26-27 0.2925393814128938 1105 28-29 0.35177539346556586 1105 30-31 0.3497701356387992 1105 32-33 0.3034553015935586 1105 34-35 0.3769596119724383 1105 36-37 0.4184563929895688 1105 38-39 0.4423201789790099 1105 40-41 0.47495235874939823 1105 42-43 0.352406647460505 1105 44-45 0.23136442046199335 1105 46-47 0.11029738389260046 1105 48-49 0.06990113436260259 1105 50-51 0.049740149667371725 1105 52-53 0.02839245939602364 1105 54-55 0.13064940255516433 1105 56-57 0.2973418052737742 1105 58-59 0.25343639566859366 1105 60-61 0.20407876941219882 1105 62-63 0.315724932193568 1105 64-65 0.3397138347159583 1105 66-67 0.3622477029325353 1105 68-69 0.4425653840939674 1105 70-71 0.4888559011551621 1105 72-73 0.39478347054162555 1105 74-75 0.41574037347098525 1105 76-77 0.34823164438514453 1105 78-79 0.2958490245265075 1105 80-81 0.4219883915581413 1105 82-83 0.27421033959772245 1105 84-85 0.469275801558517 1105 86-87 0.3478945287672168 1105 88-89 0.39484496371162336 1105 90-91 0.3099263003251842 1105 92-93 0.4353597841906236 1105 94-95 0.6565596577966168 1105 96-97 0.49768711083676465 1105 98-99 0.5093507599595313 1105 100-101 0.6487599115767111 1106 1 0.04734188653035076 1106 2 0.09402359212412392 1106 3 6.534988951898413E-4 1106 4 0.07783138833716663 1106 5 0.007262213231115311 1106 6 0.04797782527408856 1106 7 0.09086080193700496 1106 8 -0.04946382720216036 1106 9 0.056863765705088554 1106 10-11 -0.41706345199061445 1106 12-13 -0.1499862898289237 1106 14-15 -0.0684257746749779 1106 16-17 -0.024587729973312378 1106 18-19 0.015008309946139775 1106 20-21 0.024168598257389817 1106 22-23 -0.057862390809823694 1106 24-25 -0.07477016370809508 1106 26-27 0.0352676261443321 1106 28-29 -0.13252746033074914 1106 30-31 -0.0065372375233252455 1106 32-33 0.001488671317545709 1106 34-35 -0.16881636472253803 1106 36-37 -0.1269354222608854 1106 38-39 0.028561800792722636 1106 40-41 0.12453162329322964 1106 42-43 0.0776137786636042 1106 44-45 0.06013843247115602 1106 46-47 0.25137290289432457 1106 48-49 0.11075413907308729 1106 50-51 0.18137665695287808 1106 52-53 0.0673801767197233 1106 54-55 -0.1263666288613905 1106 56-57 -0.06806404088818852 1106 58-59 0.11060944332124478 1106 60-61 -0.04467619187359162 1106 62-63 0.20363537281382094 1106 64-65 0.1421476503384227 1106 66-67 -0.09849440026630418 1106 68-69 -0.4292741108750917 1106 70-71 -0.1690202527683411 1106 72-73 -0.033052512094226216 1106 74-75 -0.003066599922728841 1106 76-77 0.16172209458961007 1106 78-79 0.2978680788318151 1106 80-81 0.1287585877938966 1106 82-83 0.03679434533084702 1106 84-85 0.028791658356226435 1106 86-87 0.10293960601270769 1106 88-89 0.00168137438749838 1106 90-91 -0.04594778785105902 1106 92-93 -0.0050259840030619785 1106 94-95 -0.08124766176456077 1106 96-97 0.227143428367242 1106 98-99 -0.06304224474584075 1106 100-101 -0.3717276753905061 1107 1 0.21472687896566 1107 2 0.19512537904245875 1107 3 0.14617801914024398 1107 4 0.0012287227945222412 1107 5 0.10380780380208421 1107 6 0.14832495369712007 1107 7 -0.08223965431030678 1107 8 0.05226032363201227 1107 9 0.11538142425519027 1107 10-11 0.13591304327007947 1107 12-13 0.08824655394203518 1107 14-15 0.12753243279141202 1107 16-17 0.13032041099573632 1107 18-19 0.1933371783374156 1107 20-21 0.16278784842232596 1107 22-23 0.21662399600552362 1107 24-25 0.293157598623246 1107 26-27 0.30238329771675865 1107 28-29 0.3497628099098975 1107 30-31 0.23106503427396063 1107 32-33 0.27905814950958074 1107 34-35 0.31499758660995525 1107 36-37 0.2814585480998133 1107 38-39 0.36129848074437376 1107 40-41 0.28379679450003437 1107 42-43 0.365197690966788 1107 44-45 0.31867644217646074 1107 46-47 0.29325596655674957 1107 48-49 0.09947530193277743 1107 50-51 0.008437344194248908 1107 52-53 0.0904256269087611 1107 54-55 0.22937289180251952 1107 56-57 0.29999963722461587 1107 58-59 0.1491237027697494 1107 60-61 0.14403200708296993 1107 62-63 0.26043851292705966 1107 64-65 0.2269283177549184 1107 66-67 0.12939544566603445 1107 68-69 0.16139269967400338 1107 70-71 0.14889810650849 1107 72-73 0.3049291168238213 1107 74-75 0.2236982592571728 1107 76-77 0.24899088989537432 1107 78-79 0.12266454917380898 1107 80-81 0.25019185471438377 1107 82-83 0.14144563444254388 1107 84-85 0.04563326529116907 1107 86-87 0.3020329294577415 1107 88-89 0.3497030272441073 1107 90-91 0.48129077283219956 1107 92-93 0.4744142795625059 1107 94-95 0.4449764795337785 1107 96-97 0.5186848316216413 1107 98-99 0.5997115924608494 1107 100-101 0.4277450051753 1108 1 0.2736377452986183 1108 2 0.243229710038122 1108 3 0.22102364761038729 1108 4 0.0879359729563447 1108 5 0.12564978172978414 1108 6 0.13612102762077427 1108 7 0.12850363219212113 1108 8 0.19018222611188662 1108 9 0.2388434316379744 1108 10-11 0.18870856429468574 1108 12-13 0.1327651408588295 1108 14-15 0.17968384402223592 1108 16-17 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-0.8913745805082272 2316 56-57 -0.7680286381908843 2316 58-59 -1.04041511297239 2316 60-61 -0.7106826782731517 2316 62-63 -0.7819345101004131 2316 64-65 -0.7065063770813609 2316 66-67 -0.8545686990248669 2316 68-69 -0.9455833629288932 2316 70-71 -0.9027930858328936 2316 72-73 -0.9101592517850072 2316 74-75 -1.0423319883696145 2316 76-77 -0.9370805794866826 2316 78-79 -0.8899127416173194 2316 80-81 -0.7849488452090121 2316 82-83 -0.9293101964890091 2316 84-85 -0.8808313978458315 2316 86-87 -0.8686921121237496 2316 88-89 -0.8981295506391938 2316 90-91 -0.9615142533440384 2316 92-93 -1.1389492506110557 2316 94-95 -0.9846813883619241 2316 96-97 -1.1922630571388737 2316 98-99 -1.0346661452519754 2316 100-101 -0.9052310235698897 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5510.0 3 1381.0 4 1401.0 5 1516.0 6 1646.0 7 1998.0 8 2528.0 9 3148.0 10 4034.0 11 4984.0 12 6436.0 13 8243.0 14 9639.0 15 11936.0 16 14715.0 17 20467.0 18 18140.0 19 14111.0 20 13389.0 21 13625.0 22 14510.0 23 15750.0 24 17676.0 25 19783.0 26 22413.0 27 26088.0 28 30810.0 29 36180.0 30 43749.0 31 53736.0 32 67361.0 33 89009.0 34 122799.0 35 182603.0 36 278469.0 37 399392.0 38 326194.0 39 42929.0 40 16.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.583930430332835 18.293246587906605 13.79807340140497 23.324749580355586 2 15.59293120588936 19.601710198749497 40.76172244086401 24.04363615449713 3 19.641403592743025 23.79885404860202 29.45162882349709 27.108113535157862 4 12.475430893465479 15.976762902408195 36.7898456081711 34.75796059595523 5 13.214062083443986 37.731890873432725 34.407096483554525 14.646950559568761 6 32.72668697613167 35.77737140578732 17.102597395257163 14.393344222823846 7 29.042447626150253 32.888281447422756 19.952243595669977 18.117027330757015 8 26.710929572372883 35.092517655178646 19.287948877011498 18.908603895436972 9 26.63871056862724 16.381585332600025 20.022605206386768 36.95709889238597 10-11 25.254145310790122 26.494290703249863 26.917816996884998 21.33374698907502 12-13 26.117068380962806 23.871126693441624 27.52076266755865 22.49104225803692 14-15 22.61681187106979 25.39862524425409 26.066899301837715 25.917663582838408 16-17 21.862762569518235 28.66223523496637 27.021556109413186 22.453446086102208 18-19 21.823869284086843 26.8424743520709 29.677490756525636 21.656165607316616 20-21 22.485351468448673 26.007034377018467 30.024102821583902 21.483511332948954 22-23 22.03136776554425 26.1677013449748 29.83532886078387 21.965602028697077 24-25 21.78630221996414 26.169515800872862 30.22145700766804 21.82272497149496 26-27 21.42067106262325 26.606862345542524 29.84479073495268 22.12767585688154 28-29 21.47682495398067 27.042826737229635 29.759687684899088 21.720660623890605 30-31 21.66657934989065 26.545481254115444 29.719317655349386 22.06862174064452 32-33 21.204472052496843 26.569440392493643 29.835212867808703 22.390874687200814 34-35 21.991145942480987 26.806457842810943 29.636515205680404 21.565881009027667 36-37 22.100424373551448 26.26596215948998 29.5347812967469 22.098832170211676 38-39 21.61604844643509 26.7086954758099 29.565485509251864 22.109770568503144 40-41 22.14400184031848 26.469828584397654 29.626585824104783 21.759583751179083 42-43 21.70001815134111 26.502395745962353 29.49798319858466 22.299602904111882 44-45 21.735668503692448 26.87798880329792 29.552054910557295 21.83428778245234 46-47 22.158961681722385 27.012858327364953 28.870269257328275 21.95791073358439 48-49 21.89084266517573 27.122142207497664 28.82034371163073 22.166671415695877 50-51 22.153527138180483 27.459000928474598 28.566881359839225 21.820590573505694 52-53 22.30771106636017 27.154186673032765 28.650553760811782 21.887548499795283 54-55 21.889265179376586 27.250681596896627 28.809299443402107 22.050753780324676 56-57 21.926923005838272 27.527471511975342 28.46063528962397 22.084970192562416 58-59 21.932149598824992 27.11849614688206 28.504576459916127 22.444777794376826 60-61 21.685844507393686 27.324630251658622 28.83686663389427 22.152658607053425 62-63 21.77502921693481 27.254465448808823 29.050963626231663 21.919541708024703 64-65 21.62233896458661 27.252000765961338 28.813956103022825 22.311704166429227 66-67 21.493430608597535 27.391753077433062 28.6973408524539 22.417475461515497 68-69 22.129001953330544 26.984709605714713 28.75210156292112 22.134186878033628 70-71 21.885952976334124 27.081020574779334 28.499355594008826 22.533670854877716 72-73 21.78967468908899 27.292546710461117 28.51110838408427 22.40667021636562 74-75 21.78283296199858 27.597167638708868 28.331262946336544 22.288736452956005 76-77 21.908052650911642 27.258775087768207 28.435075657642273 22.39809660367788 78-79 21.99661399432654 27.44974635757986 28.261289166732546 22.292350481361055 80-81 22.100726212516687 27.474873375467503 28.041022934742514 22.383377477273292 82-83 22.03362552046555 27.583024859393124 28.157670675865308 22.225678944276016 84-85 22.02773060582477 27.627343738770282 28.055099807182504 22.289825848222446 86-87 22.088536576351643 27.632120623526802 27.876353082729 22.402989717392554 88-89 22.12585772348435 27.56763778838212 27.79515739083128 22.51134709730224 90-91 22.134119725361913 27.604696783735346 27.82220419146609 22.438979299436653 92-93 22.295997229500884 27.274192898311235 27.993875738801904 22.435934133385977 94-95 22.133884670580358 27.15448920550382 28.09930130226207 22.61232482165375 96-97 22.404810154336655 26.77115642988944 28.0492291639869 22.774804251787003 98-99 22.173428965378434 26.971384064115465 27.76278981050982 23.092397159996274 100-101 22.498446687247256 26.46438768629792 28.07417752828803 22.962988098166797 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1330.0 1 874.0 2 634.5 3 816.5 4 621.0 5 590.0 6 1203.5 7 2434.0 8 3784.0 9 4183.0 10 3324.0 11 2478.0 12 2336.0 13 2437.5 14 2578.5 15 2636.0 16 2660.0 17 2871.5 18 3172.0 19 3494.5 20 3925.0 21 4623.0 22 5498.0 23 6558.5 24 8082.5 25 10078.0 26 12711.5 27 15693.0 28 18041.0 29 20152.0 30 23026.5 31 27426.0 32 33827.0 33 37339.5 34 38709.0 35 42009.0 36 46349.5 37 50336.5 38 52851.5 39 54725.0 40 56958.0 41 59838.5 42 61184.0 43 62430.0 44 64558.0 45 67998.0 46 69869.5 47 70775.0 48 72266.0 49 71299.5 50 69849.0 51 68086.5 52 65981.5 53 65145.5 54 64967.5 55 60718.0 56 55111.0 57 50620.5 58 46379.0 59 40984.0 60 33098.0 61 27203.0 62 22858.0 63 16913.5 64 11754.0 65 8882.0 66 6532.5 67 5038.5 68 4096.0 69 3143.5 70 2367.5 71 1643.5 72 1163.0 73 897.5 74 654.5 75 445.0 76 289.0 77 199.5 78 133.5 79 78.0 80 41.0 81 28.0 82 21.5 83 16.0 84 12.0 85 6.5 86 5.5 87 6.0 88 2.5 89 1.5 90 1.5 91 1.5 92 2.0 93 1.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.5 99 0.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.5013565575158829 2 0.04814419031018614 3 0.057023662510252454 4 0.06441466827215736 5 0.07940198551157565 6 0.107528868549936 7 0.06929067901785851 8 0.05183969319113859 9 0.05004326817956449 10-11 0.07419235297801073 12-13 0.1074005524776807 14-15 0.05435468820734235 16-17 0.05969263681316256 18-19 0.05797320144494163 20-21 0.07424367940691284 22-23 0.06423502577099995 24-25 0.08871773235730995 26-27 0.06659604150049736 28-29 0.06703231614616535 30-31 0.07083047188492203 32-33 0.0707278190271178 34-35 0.07237026475198556 36-37 0.06808450793865876 38-39 0.039059412394511356 40-41 0.042344303844246874 42-43 0.04103547990724288 44-45 0.0215057737099872 46-47 0.02817820946726246 48-49 0.028101219823909285 50-51 0.02543224552099918 52-53 0.0254835719499013 54-55 0.025586224807705536 56-57 0.024534033015212127 58-59 0.021095162278770258 60-61 0.027536629105985994 62-63 0.019786338341766265 64-65 0.022326996572421078 66-67 0.015526244742890521 68-69 0.018426187975860153 70-71 0.040239920259260056 72-73 0.03751961952744783 74-75 0.03133478484474268 76-77 0.021942048355655195 78-79 0.015192622955026755 80-81 0.01696338475214981 82-83 0.02148011049553614 84-85 0.018374861546958037 86-87 0.00872549291335996 88-89 0.0405222156182217 90-91 0.052558263195768236 92-93 0.03338784200082738 94-95 0.05381576070387011 96-97 0.04365312778125087 98-99 0.032874577711806206 100-101 0.16822237072668986 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1948314.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.782911911804064 #Duplication Level Percentage of deduplicated Percentage of total 1 80.91276770093341 49.181136317061004 2 11.543037054109192 14.032388089092185 3 3.3562137671224836 6.120013372925701 4 1.4111704778969063 3.431002034021844 5 0.7119038445179409 2.1635794335504324 6 0.43588141776763223 1.5896485092097352 7 0.2909351850534867 1.2378721407605346 8 0.21362879424893527 1.0387984146126357 9 0.14896195592538508 0.814890730070045 >10 0.8693819804044381 9.807063416004471 >50 0.06746181052202757 2.791498910550009 >100 0.03349774050522148 3.6071395561042343 >500 0.0033824727822619636 1.4363488636820614 >1k 0.0015221127520178815 1.5077936631280453 >5k 1.6912363911309795E-4 0.7170179512744582 >10k+ 8.456181955654897E-5 0.5238085979525451 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10191 0.5230676369414786 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8499 0.4362233192390959 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5451 0.27978036394544203 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 2485 0.12754617582176178 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 2446 0.1255444450945792 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 2255 0.11574109717427479 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2085 0.10701560426091483 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 2009 0.1031147956643539 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 5.132642890211742E-5 2 0.0 0.0 0.0 0.0 5.132642890211742E-5 3 0.0 0.0 0.0 0.0 5.132642890211742E-5 4 0.0 0.0 0.0 0.0 5.132642890211742E-5 5 0.0 0.0 0.0 0.0 5.132642890211742E-5 6 0.0 0.0 0.0 0.0 5.132642890211742E-5 7 0.0 0.0 0.0 0.0 5.132642890211742E-5 8 0.0 0.0 0.0 0.0 5.132642890211742E-5 9 0.0 0.0 0.0 0.0 1.0265285780423484E-4 10-11 0.0 0.0 0.0 0.0 1.0265285780423484E-4 12-13 0.0 2.566321445105871E-5 0.0 2.566321445105871E-5 3.079585734127045E-4 14-15 0.0 5.132642890211742E-5 0.0 5.132642890211742E-5 3.5928500231482193E-4 16-17 0.0 5.132642890211742E-5 0.0 5.132642890211742E-5 3.5928500231482193E-4 18-19 2.566321445105871E-5 5.132642890211742E-5 0.0 5.132642890211742E-5 4.1061143121693934E-4 20-21 5.132642890211742E-5 7.698964335317613E-5 0.0 3.079585734127045E-4 4.619378601190568E-4 22-23 5.132642890211742E-5 1.0265285780423484E-4 0.0 4.8760107457011554E-4 4.619378601190568E-4 24-25 5.132642890211742E-5 1.2831607225529355E-4 0.0 8.212228624338788E-4 4.619378601190568E-4 26-27 5.132642890211742E-5 1.5397928670635226E-4 0.0 0.0014371400092592877 7.185700046296439E-4 28-29 1.2831607225529355E-4 1.5397928670635226E-4 0.0 0.004696368244543744 7.698964335317613E-4 30-31 1.5397928670635226E-4 1.7964250115741097E-4 0.0 0.015243949383928874 7.698964335317613E-4 32-33 1.5397928670635226E-4 2.0530571560846967E-4 0.0 0.03836650560433277 7.9555964798282E-4 34-35 1.7964250115741097E-4 2.0530571560846967E-4 0.0 0.07298618189881098 8.725492913359962E-4 36-37 2.566321445105871E-4 2.0530571560846967E-4 0.0 0.13042045584028036 8.725492913359962E-4 38-39 3.079585734127045E-4 2.0530571560846967E-4 0.0 0.21703380461260352 8.982125057870548E-4 40-41 3.079585734127045E-4 2.0530571560846967E-4 0.0 0.3064957701889942 9.238757202381136E-4 42-43 3.3362178786376325E-4 2.0530571560846967E-4 0.0 0.390773766446271 9.238757202381136E-4 44-45 3.5928500231482193E-4 2.0530571560846967E-4 0.0 0.4918098417400891 9.238757202381136E-4 46-47 3.5928500231482193E-4 2.0530571560846967E-4 0.0 0.6121703175155544 9.495389346891723E-4 48-49 3.5928500231482193E-4 2.0530571560846967E-4 0.0 0.7434376594327198 9.75202149140231E-4 50-51 3.5928500231482193E-4 2.0530571560846967E-4 0.0 0.8801199395990584 9.75202149140231E-4 52-53 3.5928500231482193E-4 2.0530571560846967E-4 0.0 1.0066909132716801 0.0010265285780423484 54-55 3.5928500231482193E-4 2.0530571560846967E-4 0.0 1.1441944163004525 0.0010265285780423484 56-57 3.5928500231482193E-4 2.0530571560846967E-4 0.0 1.3046408330484716 0.0010521917924934071 58-59 3.5928500231482193E-4 2.566321445105871E-4 0.0 1.4718110119826682 0.0011035182213955245 60-61 3.5928500231482193E-4 2.566321445105871E-4 0.0 1.6538401920840275 0.0011291814358465832 62-63 3.5928500231482193E-4 2.566321445105871E-4 0.0 1.8268102574841634 0.0011291814358465832 64-65 3.5928500231482193E-4 2.566321445105871E-4 0.0 2.004502354343294 0.0011291814358465832 66-67 3.5928500231482193E-4 2.566321445105871E-4 0.0 2.2028533388355265 0.0011291814358465832 68-69 3.5928500231482193E-4 2.566321445105871E-4 0.0 2.4253534081262056 0.0011291814358465832 70-71 4.106114312169394E-4 2.566321445105871E-4 0.0 2.65888865963084 0.0011291814358465832 72-73 4.619378601190568E-4 2.566321445105871E-4 0.0 2.897428237953431 0.0011291814358465832 74-75 4.619378601190568E-4 2.822953589616458E-4 0.0 3.125368908707734 0.0011291814358465832 76-77 4.619378601190568E-4 3.079585734127045E-4 0.0 3.367065062407805 0.0011291814358465832 78-79 4.619378601190568E-4 3.079585734127045E-4 0.0 3.6400960009526186 0.0011291814358465832 80-81 5.645907179232916E-4 3.079585734127045E-4 0.0 3.934093785703947 0.0011291814358465832 82-83 5.645907179232916E-4 3.079585734127045E-4 0.0 4.224601373289932 0.001154844650297642 84-85 5.645907179232916E-4 3.079585734127045E-4 0.0 4.516340795169567 0.0012061710791997594 86-87 5.902539323743503E-4 3.079585734127045E-4 0.0 4.82062952891577 0.001231834293650818 88-89 6.15917146825409E-4 3.079585734127045E-4 0.0 5.140649813120472 0.001231834293650818 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5105 0.0 50.63767 1 GTATCAA 8165 0.0 35.990765 1 ATCAACG 10375 0.0 28.119867 3 TATCAAC 10525 0.0 27.8094 2 TCAACGC 10465 0.0 27.787226 4 CAACGCA 10680 0.0 27.183348 5 CGCGACG 35 0.00840083 27.138325 90-91 GTACATG 8545 0.0 26.785244 1 AACGCAG 10875 0.0 26.695925 6 TACATGG 8560 0.0 26.255505 2 CGCGCGT 110 9.424636E-5 25.931398 7 ACATGGG 8420 0.0 25.676292 3 GTATAGG 525 0.0 25.484196 1 ACGCAGA 12165 0.0 23.760677 7 CGCAGAG 12225 0.0 23.68295 8 TACCGTA 145 2.3199356E-5 22.950777 7 GAGTACT 8105 0.0 22.141314 12-13 GCAGAGT 13195 0.0 21.221361 9 GTACTTT 8435 0.0 21.062134 14-15 GTACCGT 185 6.537768E-6 20.547132 6 >>END_MODULE