FastQCFastQC Report
Thu 26 May 2016
SRR937937_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937937_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1948314
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95990.49268239103142514No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86350.4432037135697839No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55920.2870173904206406No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA25730.13206290156514813No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA24820.12739219653505543No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA22110.11348273430258163No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA21560.11065978071296514No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21280.10922264070370588No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA48750.050.7721941
GTATCAA81600.038.30881
ATCAACG101750.030.297463
TATCAAC102600.030.2826042
TCAACGC102600.030.1375054
CAACGCA103750.029.7576685
AACGCAG108250.028.5645086
GTACATG83800.025.6813011
ACGCAGA122150.025.3140247
CGCAGAG122650.025.17218
TACATGG83400.024.608242
GAGTACT81300.023.1646812-13
ACATGGG82500.023.0880033
GCAGAGT135600.022.5229349
GTACTTT85300.021.8835214-15
TACCTGG15050.021.149552
CAGAGTA130200.020.75749610-11
TAAGGTG10600.020.612265
GGACCGA4650.020.4291696
GTAAGGT9100.020.3562094
Produced by FastQC (version 0.11.5)