##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937937_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1948314 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.48822007130268 34.0 31.0 34.0 31.0 34.0 2 32.70097119868768 34.0 31.0 34.0 31.0 34.0 3 32.75659005683889 34.0 31.0 34.0 31.0 34.0 4 36.22055120478527 37.0 35.0 37.0 35.0 37.0 5 36.18550654565948 37.0 35.0 37.0 35.0 37.0 6 36.22997319733883 37.0 37.0 37.0 35.0 37.0 7 36.19190438502213 37.0 37.0 37.0 35.0 37.0 8 36.184127404514875 37.0 37.0 37.0 35.0 37.0 9 37.89400168555993 39.0 38.0 39.0 35.0 39.0 10-11 37.8760133120226 39.0 38.0 39.0 35.0 39.0 12-13 37.8277012842899 39.0 38.0 39.0 35.0 39.0 14-15 39.1425232277754 40.0 38.5 41.0 36.0 41.0 16-17 39.08996470794749 40.0 38.0 41.0 36.0 41.0 18-19 39.125202354445946 40.0 38.5 41.0 36.0 41.0 20-21 39.054453234950834 40.0 39.0 41.0 35.5 41.0 22-23 38.91096917642639 40.0 38.0 41.0 35.0 41.0 24-25 38.87514948822418 40.0 38.0 41.0 35.0 41.0 26-27 38.83300843703839 40.0 38.0 41.0 35.0 41.0 28-29 38.77828958781798 40.0 38.0 41.0 35.0 41.0 30-31 38.58248208450999 40.0 38.0 41.0 34.5 41.0 32-33 38.40356995843586 40.0 38.0 41.0 34.0 41.0 34-35 38.20625525454316 40.0 38.0 41.0 34.0 41.0 36-37 38.07717339196864 40.0 38.0 41.0 33.5 41.0 38-39 38.06225433887967 40.0 38.0 41.0 33.5 41.0 40-41 38.00008135238981 40.0 38.0 41.0 33.0 41.0 42-43 37.84529418769254 40.0 37.5 41.0 33.0 41.0 44-45 37.63610229151975 40.0 37.0 41.0 33.0 41.0 46-47 37.30384835298622 40.0 37.0 41.0 32.5 41.0 48-49 36.94918375580117 40.0 36.5 41.0 31.0 41.0 50-51 36.6574925807647 40.0 36.0 41.0 31.0 41.0 52-53 36.24647952024161 39.0 35.0 41.0 30.0 41.0 54-55 36.03329417126808 39.0 35.0 41.0 29.5 41.0 56-57 35.79237509970159 39.0 35.0 40.5 29.0 41.0 58-59 35.46473027448348 38.5 35.0 40.0 28.0 41.0 60-61 35.11649071966839 38.0 34.5 40.0 28.0 41.0 62-63 34.75993217725684 37.5 34.0 40.0 26.5 41.0 64-65 34.381054080605075 37.0 34.0 40.0 26.0 41.0 66-67 33.96729890561788 36.5 34.0 39.0 26.0 41.0 68-69 33.513267368606904 36.0 33.0 39.0 25.0 40.5 70-71 32.998835659960356 35.5 32.5 38.5 23.5 40.0 72-73 32.58396978105172 35.0 32.0 38.0 23.0 40.0 74-75 32.13483196240442 35.0 32.0 37.0 22.0 39.0 76-77 30.828729096028667 34.0 30.5 35.5 20.5 38.5 78-79 31.54567179622997 35.0 31.5 36.0 22.0 39.0 80-81 31.382162218205075 35.0 32.0 36.0 20.5 37.5 82-83 31.005258649273166 35.0 31.0 35.5 19.5 37.0 84-85 30.613734490436347 34.0 31.0 35.0 18.0 37.0 86-87 30.184173854933036 34.0 31.0 35.0 13.0 36.0 88-89 29.708946042578354 34.0 30.0 35.0 7.0 36.0 90-91 29.354645606406358 34.0 30.0 35.0 3.5 35.5 92-93 28.94505018184954 34.0 29.0 35.0 2.0 35.0 94-95 28.522838207804284 34.0 29.0 35.0 2.0 35.0 96-97 27.939701198061503 34.0 29.0 35.0 2.0 35.0 98-99 27.244169830941008 33.0 27.0 35.0 2.0 35.0 100-101 24.783653456270393 31.0 21.5 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.17689190927331566 1101 2 0.09417127067338527 1101 3 0.09805172542741758 1101 4 0.053395199413735384 1101 5 0.11890205117917674 1101 6 0.08499236678899535 1101 7 0.0994995873161102 1101 8 0.09692558974592913 1101 9 -0.013450295485277763 1101 10-11 0.1162031778154784 1101 12-13 0.14118546873250182 1101 14-15 0.2029622378864815 1101 16-17 0.2020633682173809 1101 18-19 0.21559228374336215 1101 20-21 0.22396253133783262 1101 22-23 0.23051715667366324 1101 24-25 0.23248629710636948 1101 26-27 0.17431793765639725 1101 28-29 0.1910917060727897 1101 30-31 0.26289054824785296 1101 32-33 0.27138656615078816 1101 34-35 0.1459959035327998 1101 36-37 0.27376247670715514 1101 38-39 0.26189175724559277 1101 40-41 0.42968132683775906 1101 42-43 0.41511508437019984 1101 44-45 0.43348559493580296 1101 46-47 0.5031245870911647 1101 48-49 0.5485131471813176 1101 50-51 0.5726108098672924 1101 52-53 0.6250139762689386 1101 54-55 0.8575917103263606 1101 56-57 0.818617616175807 1101 58-59 0.8232135137598178 1101 60-61 0.760846103486756 1101 62-63 0.6727592131133733 1101 64-65 0.8799987203009536 1101 66-67 0.7137747772772727 1101 68-69 0.7277215606313163 1101 70-71 0.9625138317462145 1101 72-73 0.788604188487362 1101 74-75 0.6399959194498877 1101 76-77 0.5716143681721277 1101 78-79 0.7493145719004808 1101 80-81 0.9089349221448089 1101 82-83 0.8104876436395969 1101 84-85 0.8063490799756643 1101 86-87 0.9540987400231487 1101 88-89 1.0057145002994226 1101 90-91 1.1189670870825559 1101 92-93 1.0087844615044972 1101 94-95 0.9233192242333104 1101 96-97 1.055236715584968 1101 98-99 0.9972108457127007 1101 100-101 1.016096631624098 1102 1 0.14904331760612877 1102 2 0.09811359814125353 1102 3 0.16618930153574496 1102 4 0.06995309435195907 1102 5 0.11462432096494979 1102 6 0.09759119401532956 1102 7 0.06196975380979097 1102 8 0.12382596616227914 1102 9 0.03712138384817365 1102 10-11 0.18116220008957384 1102 12-13 0.21032357335424479 1102 14-15 0.18706378898966847 1102 16-17 0.1617719923128078 1102 18-19 0.16230838594494656 1102 20-21 0.11083584834543814 1102 22-23 0.229451161848786 1102 24-25 0.09972540040323707 1102 26-27 0.050868502759207956 1102 28-29 0.0574127863446563 1102 30-31 0.12472673778567867 1102 32-33 0.13677916074512098 1102 34-35 0.17241888406150707 1102 36-37 0.21177365201577203 1102 38-39 0.24198013377673533 1102 40-41 0.38594026493233713 1102 42-43 0.42732769025224115 1102 44-45 0.5153905270899912 1102 46-47 0.39267425061226646 1102 48-49 0.4306083202515296 1102 50-51 0.471972049984565 1102 52-53 0.45647559088938294 1102 54-55 0.2765961321422452 1102 56-57 0.30079971894176794 1102 58-59 0.2734289248284725 1102 60-61 0.16135165885631153 1102 62-63 0.0878386455712743 1102 64-65 0.2216856346913474 1102 66-67 0.22380726530799677 1102 68-69 0.5481489629759082 1102 70-71 0.546426696384593 1102 72-73 0.16946716002416196 1102 74-75 0.12381348427050654 1102 76-77 0.28370608557767696 1102 78-79 0.19511384680876986 1102 80-81 0.39538758073470603 1102 82-83 0.36647718337854585 1102 84-85 0.3167217536796123 1102 86-87 0.19820707092515732 1102 88-89 0.009948287373088505 1102 90-91 -0.034642587821906545 1102 92-93 0.12083468701949585 1102 94-95 0.22913239354414117 1102 96-97 0.11605073386531117 1102 98-99 0.07251324626369282 1102 100-101 0.23904140795052342 1103 1 0.12168294806348001 1103 2 0.0896511461808629 1103 3 0.13220390744730537 1103 4 0.0578590348997281 1103 5 0.008699903284508537 1103 6 0.06441814856120942 1103 7 0.1346802163926668 1103 8 0.04391189979047283 1103 9 0.05267990567756442 1103 10-11 0.024947557709978696 1103 12-13 0.06764534757041929 1103 14-15 0.09140886066336407 1103 16-17 0.12032000387036135 1103 18-19 0.07757691684457768 1103 20-21 0.08143759413525942 1103 22-23 0.20071505943909074 1103 24-25 0.1553847235941035 1103 26-27 0.13624751307310845 1103 28-29 0.18424027856013225 1103 30-31 0.06250901432551359 1103 32-33 0.08979911062495916 1103 34-35 0.002445744164553787 1103 36-37 0.034231059232695316 1103 38-39 0.02535714302464953 1103 40-41 0.05284225945278109 1103 42-43 0.11672881982594419 1103 44-45 0.0238757715660185 1103 46-47 0.010932341370889276 1103 48-49 0.15022005266973082 1103 50-51 0.1496496445910438 1103 52-53 0.03677187021575179 1103 54-55 0.13800536416304254 1103 56-57 0.11760224458627277 1103 58-59 0.2614161002396713 1103 60-61 0.22903841722291673 1103 62-63 0.3044394282311984 1103 64-65 0.15611916470266607 1103 66-67 0.21305562658456978 1103 68-69 0.2607306653781265 1103 70-71 0.28055375472408883 1103 72-73 -0.03758063646555598 1103 74-75 0.056203869802985196 1103 76-77 -0.07888660398592506 1103 78-79 -0.021065566523571277 1103 80-81 0.1547962143058612 1103 82-83 0.25722739610869283 1103 84-85 0.3523978848896938 1103 86-87 0.31461546137614604 1103 88-89 0.13756358243681177 1103 90-91 -0.04050318645856166 1103 92-93 0.04568600968671177 1103 94-95 -0.031044756900492132 1103 96-97 0.01823402309415556 1103 98-99 0.09955452058739667 1103 100-101 0.3191179440491929 1104 1 0.0858519150635928 1104 2 0.035634028825718644 1104 3 0.06259417739608608 1104 4 -0.04511787480080187 1104 5 0.06935076959911157 1104 6 0.01297349861908259 1104 7 0.05228166413797197 1104 8 0.041792681900162165 1104 9 0.08565933588764096 1104 10-11 0.09663228618587993 1104 12-13 0.11148936229992046 1104 14-15 0.1429053302917751 1104 16-17 0.017212923117881473 1104 18-19 -0.04287331469959099 1104 20-21 0.017540466300097535 1104 22-23 0.08254157763153813 1104 24-25 0.1277974426601034 1104 26-27 0.15421042731277623 1104 28-29 0.089054562331917 1104 30-31 0.07928297768305725 1104 32-33 -0.07159958976494352 1104 34-35 -0.06475388682400762 1104 36-37 -0.03815040219853216 1104 38-39 -0.06495116614458851 1104 40-41 -0.11689692308407018 1104 42-43 -0.0036202596906136364 1104 44-45 0.023145874011461842 1104 46-47 -0.14975760389646808 1104 48-49 -0.13580817150968727 1104 50-51 -0.07109019681584527 1104 52-53 -0.09432157214803993 1104 54-55 -0.13781465818989602 1104 56-57 -0.11601541069867238 1104 58-59 -0.06168544755217198 1104 60-61 -0.08921363704763507 1104 62-63 -0.1935948783357304 1104 64-65 -0.22134206311712745 1104 66-67 -0.166054296374071 1104 68-69 -0.24250274486023216 1104 70-71 -0.22916153824645136 1104 72-73 -0.1033897034458846 1104 74-75 -0.16173618850038096 1104 76-77 -0.19982988257187273 1104 78-79 -0.05694302996236189 1104 80-81 -0.06584385693169992 1104 82-83 -0.07936910347840964 1104 84-85 -0.09798718374444348 1104 86-87 -0.15699080518542274 1104 88-89 0.007258904791189735 1104 90-91 -0.09651515445693803 1104 92-93 0.047817111558412506 1104 94-95 -0.07481194030846439 1104 96-97 0.19791028685884626 1104 98-99 0.2681963694474767 1104 100-101 0.38666677063039856 1105 1 0.083401697215578 1105 2 0.11188410699772078 1105 3 0.106813492303246 1105 4 0.06565295734959165 1105 5 0.03223117285410382 1105 6 0.035314597517185575 1105 7 0.0407114235566155 1105 8 0.06950364097309603 1105 9 -0.03531585290918571 1105 10-11 -0.00715173564104532 1105 12-13 0.03852185385277096 1105 14-15 0.106365187564748 1105 16-17 0.07386361347801085 1105 18-19 0.040050231647967394 1105 20-21 0.03195642924434594 1105 22-23 0.05624137779904714 1105 24-25 0.02970368807280721 1105 26-27 0.11658634316804495 1105 28-29 -0.13280319158446474 1105 30-31 0.08557454785037066 1105 32-33 0.07018596593395188 1105 34-35 0.17905168561611617 1105 36-37 0.24687124130523586 1105 38-39 0.11974106906690452 1105 40-41 0.044722471002494046 1105 42-43 0.01960094468932283 1105 44-45 0.06601016402264293 1105 46-47 0.046610191490977115 1105 48-49 0.21701783450176748 1105 50-51 0.15493716639231536 1105 52-53 0.16661210633700563 1105 54-55 0.3239345733695558 1105 56-57 0.21205983642295934 1105 58-59 0.14136785253592876 1105 60-61 0.18648794190896467 1105 62-63 0.15599552656512827 1105 64-65 0.08908410575374859 1105 66-67 0.01359646646687196 1105 68-69 0.2590512751952332 1105 70-71 0.13843629319038797 1105 72-73 0.062376927695588336 1105 74-75 1.923611166603223E-4 1105 76-77 0.0817637062515324 1105 78-79 0.08256579084805793 1105 80-81 0.10708045085689832 1105 82-83 0.21053047265274216 1105 84-85 0.2945989971210885 1105 86-87 0.19183737368047815 1105 88-89 0.10304308570958298 1105 90-91 0.07569965250991118 1105 92-93 0.3312691660231657 1105 94-95 0.23255302623392282 1105 96-97 0.5442516149742609 1105 98-99 0.7947896324997146 1105 100-101 0.9466102355953474 1106 1 -0.15012189602819603 1106 2 -0.08171100193501246 1106 3 -0.009272343921423953 1106 4 -0.0440488653128881 1106 5 -0.035792906891828125 1106 6 -0.023323048052880324 1106 7 0.06904598455206212 1106 8 -0.05622678387133817 1106 9 -0.08325224201675496 1106 10-11 -0.03390922627751536 1106 12-13 -0.14798193927776992 1106 14-15 -0.1771087320563396 1106 16-17 -0.08534527235657663 1106 18-19 -0.03663686137181088 1106 20-21 -0.04391168223436637 1106 22-23 0.018696536576825906 1106 24-25 -0.02094498573229231 1106 26-27 -0.04482235625294351 1106 28-29 0.040202757225223706 1106 30-31 -0.004482463601291897 1106 32-33 0.06319046662257222 1106 34-35 0.03894303048307535 1106 36-37 -0.07899258465619852 1106 38-39 -0.1690021179200656 1106 40-41 -0.006732263828887142 1106 42-43 -0.16010857655314936 1106 44-45 0.055328480221149334 1106 46-47 0.054783888239590794 1106 48-49 0.12320146495150652 1106 50-51 0.16579756266067136 1106 52-53 0.11444188200872674 1106 54-55 -0.22952795828890515 1106 56-57 0.09368323130485123 1106 58-59 0.13666363382154856 1106 60-61 -0.02120357657901195 1106 62-63 0.06700081169530137 1106 64-65 -0.12959944474230412 1106 66-67 -0.32330032778710205 1106 68-69 -0.32939898709121707 1106 70-71 -0.3222415912121974 1106 72-73 -0.19845206276702498 1106 74-75 -0.2636019514518253 1106 76-77 -0.10417529407928328 1106 78-79 -0.20090736121770547 1106 80-81 -0.40276817955574273 1106 82-83 -0.31938870632913563 1106 84-85 -0.13888696032615044 1106 86-87 -0.04034837061664831 1106 88-89 0.021802854450275078 1106 90-91 -0.054304264580810724 1106 92-93 0.02323692972255742 1106 94-95 -0.36595323433115823 1106 96-97 -0.20462794612737056 1106 98-99 -0.09892160755500967 1106 100-101 -0.025978188697987292 1107 1 0.0475332066920231 1107 2 0.06116641965328995 1107 3 -0.0025835010137598147 1107 4 0.031119321413669354 1107 5 0.061931505634539974 1107 6 0.08941438510878896 1107 7 0.07678449321072378 1107 8 0.0443665862872038 1107 9 -0.07199175229987986 1107 10-11 0.05078839490661835 1107 12-13 0.07739358540422359 1107 14-15 0.06647419624555084 1107 16-17 0.08986368910441911 1107 18-19 0.13502742616514496 1107 20-21 0.11936807420841689 1107 22-23 0.04539686593722081 1107 24-25 0.06093678878222164 1107 26-27 -9.755147498111683E-4 1107 28-29 -0.022955954505611942 1107 30-31 0.0450707887088484 1107 32-33 0.0659264054348796 1107 34-35 0.14052233323753427 1107 36-37 0.17828705735660577 1107 38-39 0.14622182566063913 1107 40-41 0.1203468191186019 1107 42-43 0.14833490770946156 1107 44-45 0.20518226815535456 1107 46-47 0.3615711554174226 1107 48-49 0.2584258382481863 1107 50-51 0.36217679521208623 1107 52-53 0.20420134004515234 1107 54-55 0.27471802175440985 1107 56-57 0.15249034460091337 1107 58-59 0.30727837631425103 1107 60-61 0.21568724973340636 1107 62-63 0.3094372674462633 1107 64-65 0.24869497394734452 1107 66-67 0.26471310735511366 1107 68-69 0.27973776422225427 1107 70-71 0.4027750723523056 1107 72-73 0.37025215769561015 1107 74-75 0.21498008724143602 1107 76-77 0.1633796292948766 1107 78-79 0.27833832309411477 1107 80-81 0.2739147318230657 1107 82-83 0.3496476749216306 1107 84-85 0.33155437487378947 1107 86-87 0.29697395812524263 1107 88-89 0.3068000214632498 1107 90-91 0.22028025680854313 1107 92-93 0.3324050165039374 1107 94-95 0.3279244718836196 1107 96-97 0.29224369747103296 1107 98-99 0.5618776255226905 1107 100-101 0.5081044437517015 1108 1 -0.007360161266170451 1108 2 0.02543575826627631 1108 3 -0.0344375300279296 1108 4 -0.02052990252030895 1108 5 -0.07677502047602758 1108 6 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54-55 -0.6091938551051186 2316 56-57 -0.5439779794003883 2316 58-59 -0.43468687298425124 2316 60-61 -0.263920783853429 2316 62-63 -0.40012605280953295 2316 64-65 -0.5822309707884159 2316 66-67 -0.4338129186391342 2316 68-69 -0.45649802083210744 2316 70-71 -0.6432301417342003 2316 72-73 -0.42263634888335844 2316 74-75 -0.5927813683012744 2316 76-77 -0.3939033745606686 2316 78-79 -0.35373455138267573 2316 80-81 -0.3640981594979351 2316 82-83 -0.2761929483987089 2316 84-85 -0.35388776442527003 2316 86-87 -0.337449593294032 2316 88-89 -0.4040028081944058 2316 90-91 -0.4154141223892829 2316 92-93 -0.6531779496849097 2316 94-95 -0.5151482417690723 2316 96-97 -0.5880895258247598 2316 98-99 -0.6635535311834602 2316 100-101 -0.6411514273858749 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 4.0 4 23.0 5 31.0 6 82.0 7 158.0 8 406.0 9 877.0 10 1335.0 11 2147.0 12 3380.0 13 4880.0 14 6801.0 15 9046.0 16 11822.0 17 17119.0 18 17136.0 19 11912.0 20 9805.0 21 9643.0 22 10246.0 23 10991.0 24 12528.0 25 14608.0 26 17459.0 27 21486.0 28 25824.0 29 31738.0 30 38247.0 31 47723.0 32 59085.0 33 78232.0 34 109036.0 35 167630.0 36 276643.0 37 459106.0 38 414139.0 39 46968.0 40 14.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.88132508414968 18.638320106512605 14.231330268118999 23.249024541218716 2 15.856029929938975 19.872937388604612 40.228057675090966 24.04297500636545 3 19.70744021480528 23.723130732585183 29.707527475601626 26.861901577007906 4 12.674291720944364 16.393404759191792 36.526555781049666 34.40574773881417 5 13.195775011509959 37.79680164326779 34.484962118509706 14.522461226712547 6 32.93165270074536 35.90868822992598 16.800577319672293 14.359081749656369 7 29.039928882100114 32.860462943858124 19.86029972581422 18.239308448227547 8 26.736751446255646 35.39231753517284 19.00393521144255 18.86699580712896 9 26.444241076996594 16.343266230629975 19.961330529531537 37.251162162841894 10-11 25.288788152217762 26.55483151073184 26.839308242921827 21.317072094128562 12-13 26.075417001571616 23.913881438002292 27.514071140483516 22.496630419942576 14-15 22.5154415561352 25.488370971003647 25.986186004925283 26.01000146793587 16-17 21.852380230143105 28.67352440667153 27.066952144923583 22.40714321826178 18-19 21.791533602899737 26.859325550193653 29.683998575178332 21.665142271728275 20-21 22.3129331309019 26.04528838780607 30.105670851823678 21.53610762946835 22-23 21.96904605725771 26.186821015503657 29.87693462142139 21.967198305817234 24-25 21.749612784092005 26.313640394158206 30.16624298552127 21.770503836228524 26-27 21.388412517875143 26.688849684003664 29.875739679904346 22.046998118216845 28-29 21.48106003447083 27.04774487069333 29.764247446766795 21.706947648069047 30-31 21.64669042053796 26.554215593585017 29.747309725229094 22.051784260647924 32-33 21.269882331142455 26.587988080930796 29.81366124888461 22.328468339042136 34-35 22.018051909249678 26.75575403323925 29.649121275933833 21.577072781577236 36-37 22.110751499619273 26.277707957086495 29.553323517659102 22.05821702563513 38-39 21.58354040782445 26.660850356820088 29.640900619388226 22.11470861596723 40-41 22.14376355865295 26.466464888928133 29.676034821033483 21.71373673138544 42-43 21.67852574434491 26.523851272690692 29.554717384028795 22.242905598935607 44-45 21.738921775800858 26.893318598810527 29.577612627479198 21.790146997909414 46-47 22.15356456916082 27.04474227460255 28.835880663999745 21.96581249223688 48-49 21.901782772181484 27.095991713861316 28.853998893402196 22.148226620555004 50-51 22.14137454229657 27.410674049460198 28.601524189632677 21.846427218610554 52-53 22.348707651846674 27.17937149761281 28.598239298182943 21.873681552357578 54-55 21.888258258165777 27.304248699131662 28.777548177552486 22.02994486515007 56-57 22.00736123643314 27.521333830173166 28.368348223130358 22.102956710263335 58-59 21.902732311116175 27.156094962105698 28.538982936015444 22.40218979076268 60-61 21.700557507670734 27.411033334462516 28.75622204634366 22.13218711152309 62-63 21.758145760898913 27.321340399956064 28.960911844805302 21.95960199433972 64-65 21.51647527041329 27.37094739348996 28.71138841069766 22.401188925399087 66-67 21.473489386207767 27.50644916579155 28.57765740019319 22.44240404780749 68-69 21.991281154909505 27.156479582160625 28.61787545452889 22.234363808400982 70-71 21.775879042084593 27.198798550952258 28.370170311356386 22.655152095606766 72-73 21.724783582112533 27.405515743355537 28.340657614737662 22.52904305979426 74-75 21.696400066929662 27.756460200973766 28.119312390097285 22.427827341999286 76-77 21.771644611700168 27.389219602179114 28.386902727178477 22.452233058942245 78-79 21.85830928690139 27.589623643827434 28.203693039212364 22.34837403005881 80-81 21.943254526734396 27.627014947282625 27.961457958008822 22.468272567974157 82-83 21.86167116799448 27.738572940501378 28.093110248142754 22.30664564336139 84-85 21.89361838091897 27.74576487372447 27.994140410966818 22.366476334389745 86-87 21.938009801325048 27.75528817825518 27.79178138158854 22.51492063883123 88-89 21.890094007089733 27.852695964016 27.654524266024332 22.602685762869935 90-91 21.843420507286453 27.777263086037497 27.775081705531594 22.604234701144456 92-93 21.896375133994574 27.535066992796054 27.91621852105919 22.65233935215019 94-95 21.72778617820331 27.412573127329576 28.0809202212785 22.778720473188613 96-97 21.966086035992685 27.080332338883007 28.03510730439804 22.918474320726272 98-99 21.645304606957605 27.33699496077121 27.7645697767403 23.253130655530885 100-101 21.679051733960748 27.125889358696803 28.09980834711448 23.095250560227974 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1320.0 1 865.0 2 604.0 3 805.5 4 640.0 5 586.5 6 1153.0 7 2442.0 8 3697.5 9 4077.0 10 3283.0 11 2391.0 12 2293.5 13 2343.0 14 2381.0 15 2464.0 16 2627.0 17 2859.5 18 3139.0 19 3466.0 20 3889.0 21 4486.0 22 5297.5 23 6516.5 24 8029.5 25 9817.5 26 12683.0 27 15684.0 28 17897.0 29 20286.5 30 23299.5 31 27553.0 32 34126.5 33 37565.5 34 38503.5 35 41779.5 36 46156.5 37 50648.5 38 53440.5 39 54886.0 40 56922.0 41 59688.0 42 61162.5 43 62598.0 44 65055.5 45 68860.0 46 70648.5 47 71689.5 48 73177.5 49 71822.0 50 70064.5 51 68147.0 52 66391.0 53 66061.5 54 65744.0 55 61141.0 56 55310.5 57 50368.5 58 46030.5 59 40806.5 60 32575.5 61 26709.5 62 22430.0 63 16170.0 64 10952.5 65 8098.5 66 6011.5 67 4793.0 68 3748.5 69 2845.0 70 2126.0 71 1375.5 72 1012.5 73 853.0 74 620.0 75 396.0 76 253.5 77 145.0 78 81.0 79 44.5 80 26.0 81 15.0 82 7.5 83 4.5 84 3.5 85 2.0 86 1.0 87 0.5 88 0.5 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.015295275812830992 3 0.006723762186177382 4 0.0 5 5.132642890211742E-5 6 0.0 7 0.0 8 3.5928500231482193E-4 9 3.5928500231482193E-4 10-11 0.0 12-13 0.0 14-15 0.0 16-17 2.309689300595284E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.005722896822586093 26-27 0.021942048355655195 28-29 0.0 30-31 0.0 32-33 3.8494821676588066E-4 34-35 0.01929873726719615 36-37 0.003567186808697161 38-39 0.017630628327877335 40-41 0.015192622955026755 42-43 0.008109575766534552 44-45 0.002822953589616458 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 2.8229535896164584E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0027972903751653996 86-87 3.079585734127045E-4 88-89 1.7964250115741097E-4 90-91 3.336217878637632E-4 92-93 3.8494821676588066E-4 94-95 0.0 96-97 7.698964335317613E-5 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1948314.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.253148878889306 #Duplication Level Percentage of deduplicated Percentage of total 1 79.8306150750617 47.30215320135936 2 12.15930242185086 14.409539133307364 3 3.545442390606752 6.302358774364411 4 1.4808706989676033 3.5098500798524905 5 0.7940294510118813 2.352437263751487 6 0.4481607272100825 1.593296057463019 7 0.3105142680814826 1.2879263708956001 8 0.21433985788198218 1.0160249207808851 9 0.16031680030154632 0.854934771244922 >10 0.9401059571276688 10.286933016870895 >50 0.07494592486019748 2.9967468262501544 >100 0.036155693366605034 3.904324227370077 >500 0.0035538346756316375 1.542340031863074 >1k 0.0013868623124416134 1.4174325740711653 >5k 2.600366835828025E-4 1.2237027505550975 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9599 0.49268239103142514 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8635 0.4432037135697839 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5592 0.2870173904206406 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 2573 0.13206290156514813 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 2482 0.12739219653505543 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 2211 0.11348273430258163 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 2156 0.11065978071296514 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2128 0.10922264070370588 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 5.132642890211742E-5 0.0 6 0.0 0.0 0.0 5.132642890211742E-5 0.0 7 0.0 0.0 0.0 5.132642890211742E-5 0.0 8 0.0 0.0 0.0 5.132642890211742E-5 0.0 9 0.0 0.0 0.0 5.132642890211742E-5 1.0265285780423484E-4 10-11 0.0 0.0 0.0 5.132642890211742E-5 1.5397928670635226E-4 12-13 0.0 0.0 0.0 7.698964335317613E-5 3.3362178786376325E-4 14-15 0.0 0.0 0.0 1.0265285780423484E-4 3.849482167658806E-4 16-17 0.0 0.0 0.0 1.0265285780423484E-4 4.1061143121693934E-4 18-19 0.0 2.566321445105871E-5 0.0 1.0265285780423484E-4 5.132642890211742E-4 20-21 0.0 5.132642890211742E-5 0.0 3.5928500231482193E-4 5.645907179232916E-4 22-23 0.0 7.698964335317613E-5 0.0 5.389275034722329E-4 5.645907179232916E-4 24-25 5.132642890211742E-5 1.0265285780423484E-4 0.0 8.725492913359962E-4 5.645907179232916E-4 26-27 5.132642890211742E-5 1.2831607225529355E-4 0.0 0.0015397928670635227 6.929067901785852E-4 28-29 1.5397928670635226E-4 1.5397928670635226E-4 0.0 0.004747694673445862 7.698964335317613E-4 30-31 1.5397928670635226E-4 1.5397928670635226E-4 0.0 0.015269612598379934 7.698964335317613E-4 32-33 1.7964250115741097E-4 2.0530571560846967E-4 0.0 0.03839216881878384 7.698964335317613E-4 34-35 2.566321445105871E-4 2.0530571560846967E-4 0.0 0.07347378297338109 8.212228624338787E-4 36-37 3.079585734127045E-4 2.0530571560846967E-4 0.0 0.13101070977265472 8.212228624338787E-4 38-39 3.079585734127045E-4 2.0530571560846967E-4 0.0 0.21782936426058633 8.468860768849375E-4 40-41 3.079585734127045E-4 2.0530571560846967E-4 0.0 0.30767627805374287 9.238757202381136E-4 42-43 3.3362178786376325E-4 2.0530571560846967E-4 0.0 0.3926985075301004 9.238757202381136E-4 44-45 3.5928500231482193E-4 2.0530571560846967E-4 0.0 0.493939888539527 9.752021491402311E-4 46-47 3.849482167658806E-4 2.0530571560846967E-4 0.0 0.6142233746716392 0.0010265285780423484 48-49 4.1061143121693934E-4 2.0530571560846967E-4 0.0 0.7455420430177067 0.0010265285780423484 50-51 4.1061143121693934E-4 2.0530571560846967E-4 0.0 0.8829428931886749 0.0010265285780423484 52-53 4.1061143121693934E-4 2.0530571560846967E-4 0.0 1.0101554472225729 0.0010778550069444658 54-55 4.1061143121693934E-4 2.0530571560846967E-4 0.0 1.1484031834704262 0.0010778550069444658 56-57 4.1061143121693934E-4 2.566321445105871E-4 0.0 1.3089779162907007 0.0011035182213955245 58-59 4.1061143121693934E-4 2.566321445105871E-4 0.0 1.4766356962994671 0.0011291814358465832 60-61 4.1061143121693934E-4 2.566321445105871E-4 0.0 1.6591781406898476 0.0011291814358465832 62-63 4.1061143121693934E-4 2.566321445105871E-4 0.0 1.8322508589477877 0.0011805078647487007 64-65 4.1061143121693934E-4 2.566321445105871E-4 0.0 2.0095323443757014 0.0011805078647487007 66-67 4.1061143121693934E-4 2.566321445105871E-4 0.0 2.2076266967234233 0.0011805078647487007 68-69 4.1061143121693934E-4 2.566321445105871E-4 0.0 2.4313329370933023 0.0011805078647487007 70-71 4.1061143121693934E-4 2.566321445105871E-4 0.0 2.666690276823962 0.0012061710791997594 72-73 4.1061143121693934E-4 2.566321445105871E-4 0.0 2.9065386790835563 0.001231834293650818 74-75 4.1061143121693934E-4 2.566321445105871E-4 0.0 3.13496695091243 0.001231834293650818 76-77 4.1061143121693934E-4 2.566321445105871E-4 0.0 3.377099379258169 0.001231834293650818 78-79 4.1061143121693934E-4 2.566321445105871E-4 0.0 3.651259499238829 0.001231834293650818 80-81 4.619378601190568E-4 2.566321445105871E-4 0.0 3.9470023825728298 0.001231834293650818 82-83 4.619378601190568E-4 2.566321445105871E-4 0.0 4.239691343387154 0.001231834293650818 84-85 4.619378601190568E-4 2.566321445105871E-4 0.0 4.534920962432134 0.001231834293650818 86-87 4.8760107457011554E-4 2.566321445105871E-4 0.0 4.843546779420565 0.0012574975081018768 88-89 5.132642890211742E-4 3.079585734127045E-4 0.0 5.168905012231088 0.0012831607225529354 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4875 0.0 50.772194 1 GTATCAA 8160 0.0 38.3088 1 ATCAACG 10175 0.0 30.29746 3 TATCAAC 10260 0.0 30.282604 2 TCAACGC 10260 0.0 30.137505 4 CAACGCA 10375 0.0 29.757668 5 AACGCAG 10825 0.0 28.564508 6 GTACATG 8380 0.0 25.681301 1 ACGCAGA 12215 0.0 25.314024 7 CGCAGAG 12265 0.0 25.1721 8 TACATGG 8340 0.0 24.60824 2 GAGTACT 8130 0.0 23.16468 12-13 ACATGGG 8250 0.0 23.088003 3 GCAGAGT 13560 0.0 22.522934 9 GTACTTT 8530 0.0 21.88352 14-15 TACCTGG 1505 0.0 21.14955 2 CAGAGTA 13020 0.0 20.757496 10-11 TAAGGTG 1060 0.0 20.61226 5 GGACCGA 465 0.0 20.429169 6 GTAAGGT 910 0.0 20.356209 4 >>END_MODULE