FastQCFastQC Report
Thu 26 May 2016
SRR937936_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937936_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1978509
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98750.49911322111751827No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84600.42759471905359037No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50330.2543834776591868No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA25410.12843004504907485No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA24620.1244371392801347No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA21950.11094212864333698No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA21450.10841497309337486No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA46050.053.3386271
GTATCAA79250.038.7420161
ATCAACG100300.030.487683
TCAACGC100650.030.2399144
TATCAAC102150.029.9355282
CAACGCA102250.029.8597415
AACGCAG104800.029.084136
GTACATG85450.027.2407151
TACATGG84300.026.8530582
ACATGGG83100.025.8101123
ACGCAGA119600.025.4023577
CGCAGAG120150.025.1660848
GAGTACT79900.023.29288712-13
TAAGGTG10250.023.1985325
TGGTATC21700.023.0114482
GTAAGGT10050.022.7137854
GCAGAGT131300.022.6657419
GTGGTAT24050.022.3658331
GGTAAGG10250.022.2705923
GTGTAGC12500.022.0871521
Produced by FastQC (version 0.11.5)