FastQCFastQC Report
Thu 26 May 2016
SRR937936_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937936_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1978509
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95880.4846073482607357No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84240.4257751670576176No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52550.2656040483010186No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA24770.12519528594512333No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA24690.12479094105712939No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA22400.11321656863830289No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21920.11079049931033925No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA20900.10563510198841654No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA47500.049.8086131
GTATCAA79700.038.030421
ATCAACG98850.030.3180073
TCAACGC100350.029.9098854
TATCAAC101350.029.6691272
CAACGCA101900.029.4083215
ACCGCGC1303.0449155E-729.2240058
AACGCAG104400.028.8398386
ACGCAGA120100.025.0295937
CGCAGAG119850.025.0025568
GTACATG79650.024.932121
TACATGG79700.024.496742
TGGTATC22500.024.2795222
GAGTACT82400.022.79645212-13
ACATGGG80050.022.6647013
GCAGAGT132400.022.3456579
GTACTTT86750.021.62596914-15
TAAGGTG10250.021.3132175
GTGGTAT25700.021.258541
GTAAGGT10100.021.1595384
Produced by FastQC (version 0.11.5)