##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937936_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1978509 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.503031828513286 34.0 31.0 34.0 31.0 34.0 2 32.71503692932405 34.0 31.0 34.0 31.0 34.0 3 32.76926311682181 34.0 31.0 34.0 31.0 34.0 4 36.22739244552337 37.0 35.0 37.0 35.0 37.0 5 36.20475266981348 37.0 35.0 37.0 35.0 37.0 6 36.24010252164635 37.0 37.0 37.0 35.0 37.0 7 36.19514846786141 37.0 37.0 37.0 35.0 37.0 8 36.18228423524988 37.0 37.0 37.0 35.0 37.0 9 37.871371825955805 39.0 38.0 39.0 35.0 39.0 10-11 37.846173557967134 39.0 38.0 39.0 35.0 39.0 12-13 37.765487293714614 39.0 38.0 39.0 35.0 39.0 14-15 39.03750829538809 40.0 38.0 41.0 36.0 41.0 16-17 38.91662105150899 40.0 38.0 41.0 35.5 41.0 18-19 39.17494790268834 40.0 39.0 41.0 36.0 41.0 20-21 39.15917390317659 40.0 39.0 41.0 36.0 41.0 22-23 39.06488118072751 40.0 39.0 41.0 35.5 41.0 24-25 38.964104282568336 40.0 38.0 41.0 35.0 41.0 26-27 38.789344652968474 40.0 38.0 41.0 35.0 41.0 28-29 38.67232294621859 40.0 38.0 41.0 34.5 41.0 30-31 38.42468444672225 40.0 38.0 41.0 34.0 41.0 32-33 38.20966596563372 40.0 38.0 41.0 34.0 41.0 34-35 38.05220067232446 40.0 38.0 41.0 33.5 41.0 36-37 38.04513803070898 40.0 38.0 41.0 33.5 41.0 38-39 37.89762720311103 40.0 37.0 41.0 33.0 41.0 40-41 37.72524208886591 40.0 37.0 41.0 33.0 41.0 42-43 37.487002586290984 40.0 37.0 41.0 33.0 41.0 44-45 37.20913223038157 40.0 37.0 41.0 32.0 41.0 46-47 36.79873253040547 40.0 36.0 41.0 31.0 41.0 48-49 36.3851347150809 39.0 35.5 41.0 30.0 41.0 50-51 35.99923250285948 39.0 35.0 40.5 29.5 41.0 52-53 35.4784170807411 38.5 35.0 40.0 27.5 41.0 54-55 35.165605261335685 38.0 34.5 40.0 27.0 41.0 56-57 34.82007739161156 38.0 34.0 40.0 26.0 41.0 58-59 34.38569700719077 38.0 34.0 40.0 25.5 41.0 60-61 33.908925862859356 37.0 33.0 40.0 23.5 41.0 62-63 33.44903384316169 36.5 33.0 39.0 22.5 41.0 64-65 32.963862939213314 36.0 32.0 39.0 21.5 40.0 66-67 32.40889199897498 35.5 31.5 39.0 19.5 40.0 68-69 31.814040522433814 35.0 31.0 38.0 17.5 40.0 70-71 31.200130249597045 35.0 30.0 37.5 12.0 39.5 72-73 30.608352552351292 34.0 29.5 37.0 8.0 39.0 74-75 30.00329692713048 34.0 29.0 36.0 7.0 39.0 76-77 28.652007395468 32.5 27.0 35.0 4.0 37.0 78-79 29.331546381643953 34.0 29.0 35.0 2.0 37.0 80-81 29.00013040122638 34.0 29.0 35.0 2.0 37.0 82-83 28.426301068127565 33.5 27.5 35.0 2.0 36.5 84-85 27.815461289284002 33.0 26.5 35.0 2.0 36.0 86-87 27.156212835018692 33.0 25.5 35.0 2.0 35.5 88-89 26.387192072414123 32.0 24.5 34.5 2.0 35.0 90-91 25.817665727070235 32.0 23.5 34.0 2.0 35.0 92-93 24.982153732937277 31.0 19.0 34.0 2.0 35.0 94-95 24.13207723593878 31.0 12.0 34.0 2.0 35.0 96-97 23.045554758659172 31.0 2.0 34.0 2.0 35.0 98-99 21.569772237578903 30.0 2.0 34.0 2.0 35.0 100-101 17.22995017965549 23.0 2.0 31.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.18254856207627057 1101 2 0.15684145511689707 1101 3 0.17818576435220734 1101 4 0.12403092189091325 1101 5 0.09335038889138758 1101 6 0.10057994786381386 1101 7 0.136274223477713 1101 8 0.12202094067057345 1101 9 0.051163816661180306 1101 10-11 0.10142655939515777 1101 12-13 0.21788582575079118 1101 14-15 0.2109833809992807 1101 16-17 0.2927186130459276 1101 18-19 0.25277659805080077 1101 20-21 0.2031732143479772 1101 22-23 0.3049805710246787 1101 24-25 0.31319941722806277 1101 26-27 0.353507878066047 1101 28-29 0.33351364597899646 1101 30-31 0.37022122840954097 1101 32-33 0.41101937854145376 1101 34-35 0.2303325562535008 1101 36-37 0.4252315465647385 1101 38-39 0.41859167171591594 1101 40-41 0.35385742758692373 1101 42-43 0.4506869410223544 1101 44-45 0.5219517967580174 1101 46-47 0.42192835568702947 1101 48-49 0.5692283893538317 1101 50-51 0.4201461224387728 1101 52-53 0.2663845360318646 1101 54-55 0.17082885982727447 1101 56-57 0.44878385854895697 1101 58-59 0.3790203924201947 1101 60-61 0.5039229328706298 1101 62-63 0.4947907181348299 1101 64-65 0.36859748490802957 1101 66-67 0.22425224890758244 1101 68-69 0.39057101572647923 1101 70-71 0.4008074062609701 1101 72-73 0.2705202539393703 1101 74-75 0.2693320250990361 1101 76-77 0.25175473081412036 1101 78-79 0.31664042422887206 1101 80-81 0.13110713203158397 1101 82-83 0.05438842108184616 1101 84-85 -0.17566715969522662 1101 86-87 -0.15229723073644763 1101 88-89 -0.20954707113191162 1101 90-91 -0.16402600843605342 1101 92-93 -0.4410271438753597 1101 94-95 -0.6658740018149523 1101 96-97 -0.4602462593988932 1101 98-99 -0.2923559847750177 1101 100-101 -1.1093665245385047 1102 1 0.11767709022180384 1102 2 0.206627569442837 1102 3 0.19156696926961558 1102 4 0.1417301253705503 1102 5 0.11441480192526399 1102 6 0.11833661223413117 1102 7 0.08647749137849559 1102 8 0.13198759461673149 1102 9 0.15489727557571342 1102 10-11 0.16976645180589145 1102 12-13 0.23487832273253417 1102 14-15 0.29003301534191195 1102 16-17 0.24809788927604615 1102 18-19 0.2441131196307964 1102 20-21 0.2703731014248305 1102 22-23 0.24969929059617613 1102 24-25 0.30770175282589207 1102 26-27 0.34696857887654886 1102 28-29 0.28802398238129 1102 30-31 0.2693550367112678 1102 32-33 0.05081532367630359 1102 34-35 0.2722690132524548 1102 36-37 0.3277199466474272 1102 38-39 0.27552779456731 1102 40-41 0.3314543944013906 1102 42-43 0.3750562296752733 1102 44-45 0.33224388545514927 1102 46-47 0.3421762307270768 1102 48-49 0.40947524633678967 1102 50-51 0.30857518540779694 1102 52-53 0.3610791470122692 1102 54-55 0.5601259257617741 1102 56-57 0.49489544260205776 1102 58-59 0.4456366142542052 1102 60-61 0.36236865325219014 1102 62-63 0.568248533409303 1102 64-65 0.5261327624597456 1102 66-67 0.2748567124945467 1102 68-69 0.14607320829666293 1102 70-71 0.17318889161250084 1102 72-73 0.34857946863636613 1102 74-75 0.19433369583689242 1102 76-77 0.1432243805337805 1102 78-79 0.014686722716962919 1102 80-81 0.14952990553137013 1102 82-83 0.0073418484053746624 1102 84-85 -0.024117964116463497 1102 86-87 -0.1633582960488731 1102 88-89 -0.4979549922406079 1102 90-91 -0.6729841300645205 1102 92-93 -0.4601658363527932 1102 94-95 -0.32985176944685435 1102 96-97 -0.3359975449301764 1102 98-99 -0.30911692061306084 1102 100-101 -0.8347643631346102 1103 1 0.13824756001053373 1103 2 0.2210237626427869 1103 3 0.14714306104600894 1103 4 0.10753258460928805 1103 5 0.07608796225145653 1103 6 0.08005680734268594 1103 7 0.07976157525186522 1103 8 0.06642909503220551 1103 9 0.12034386787614437 1103 10-11 0.11429247579599888 1103 12-13 0.21039804968230413 1103 14-15 0.20278275450593952 1103 16-17 0.10674121091378197 1103 18-19 0.1845898630016336 1103 20-21 0.2276959881062126 1103 22-23 0.23172312924379668 1103 24-25 0.22811387218064283 1103 26-27 0.23429705352672414 1103 28-29 0.2752624697308548 1103 30-31 0.3716494762237943 1103 32-33 0.32476882195479817 1103 34-35 0.27161555517538716 1103 36-37 0.2748013792381485 1103 38-39 0.26357681841808045 1103 40-41 0.28480787084865256 1103 42-43 0.13257107151564185 1103 44-45 0.2784612388049936 1103 46-47 0.25424115529246194 1103 48-49 0.20520333441429983 1103 50-51 0.04751202775282337 1103 52-53 0.11146466952876466 1103 54-55 0.20196729903542376 1103 56-57 0.14621151426887025 1103 58-59 0.2539620369682183 1103 60-61 0.28978644074194904 1103 62-63 0.14411445410629398 1103 64-65 0.18045920810632055 1103 66-67 0.23004839106535258 1103 68-69 -0.04983375986197558 1103 70-71 -0.1510365061642034 1103 72-73 0.020025243892344946 1103 74-75 -0.20096347672189196 1103 76-77 -0.26326352043403745 1103 78-79 -0.27778738670605563 1103 80-81 -0.22216793474383323 1103 82-83 -0.15107026325815553 1103 84-85 -0.23913406983172436 1103 86-87 -0.3642144178328657 1103 88-89 -0.3356234632877424 1103 90-91 -0.5426881688732443 1103 92-93 -0.4742070500631641 1103 94-95 -0.5816177184534617 1103 96-97 -0.5962884742871886 1103 98-99 -0.843442549806813 1103 100-101 -1.365770342468295 1104 1 0.17331008950690574 1104 2 0.19976328333446247 1104 3 0.140897151115972 1104 4 0.115433952114671 1104 5 0.10761684025727192 1104 6 0.0726980604781744 1104 7 0.039505994453485016 1104 8 0.10619443061223421 1104 9 0.3122845856559664 1104 10-11 0.1417826737075103 1104 12-13 0.16371718721115514 1104 14-15 0.18804921024420196 1104 16-17 0.23982406207409923 1104 18-19 0.2606648826283333 1104 20-21 0.1735766892835855 1104 22-23 0.17659053494832477 1104 24-25 0.23222351252481133 1104 26-27 0.12083231795502769 1104 28-29 0.12045029475413571 1104 30-31 0.14216163464311649 1104 32-33 0.15203566403219781 1104 34-35 0.17189753769213212 1104 36-37 0.10593527334518882 1104 38-39 0.3057707122322597 1104 40-41 0.14236840388402072 1104 42-43 0.09836724551918508 1104 44-45 0.07240013527173517 1104 46-47 0.1029944512834291 1104 48-49 0.240957022376179 1104 50-51 0.4527875562658963 1104 52-53 0.2677088617702168 1104 54-55 0.17504886615312643 1104 56-57 0.2442580236440719 1104 58-59 0.343705633932025 1104 60-61 0.0772620411163416 1104 62-63 0.17131011066319246 1104 64-65 0.10113719387147313 1104 66-67 0.07671582177492553 1104 68-69 0.22331476895183044 1104 70-71 0.17879095905682973 1104 72-73 -0.06095208712370592 1104 74-75 -0.019256240691202464 1104 76-77 -0.2106338236821017 1104 78-79 -0.25702954250096965 1104 80-81 0.052663647298043514 1104 82-83 -0.1737419222975376 1104 84-85 -0.38158747541685756 1104 86-87 -0.2862937277388049 1104 88-89 -0.3902204766496702 1104 90-91 -0.35082460628574097 1104 92-93 -0.5907178669958562 1104 94-95 -0.5313816629873287 1104 96-97 -0.77997921825472 1104 98-99 -0.7514228746695935 1104 100-101 -1.1135909898735754 1105 1 0.0790786626499127 1105 2 0.10637440388487818 1105 3 0.03982748690901872 1105 4 0.014466985134788501 1105 5 0.05126886118814866 1105 6 0.10741778845418537 1105 7 0.09618894929466393 1105 8 0.12373683757714815 1105 9 0.09536976090797822 1105 10-11 0.0830282996659264 1105 12-13 0.08327030909932631 1105 14-15 0.07663992469429104 1105 16-17 0.0738336199060754 1105 18-19 0.06428197574837213 1105 20-21 0.12699946419492392 1105 22-23 0.15870965727953745 1105 24-25 0.09706992909057988 1105 26-27 0.10382571092086579 1105 28-29 0.08173881696792762 1105 30-31 0.042131040547722876 1105 32-33 0.1119953056350056 1105 34-35 0.05485334669894826 1105 36-37 0.07203685434480178 1105 38-39 -0.005668844803949469 1105 40-41 0.0633227245875645 1105 42-43 -0.09689701434028564 1105 44-45 -0.0909086188334669 1105 46-47 0.008628066290341962 1105 48-49 -0.041999378080632255 1105 50-51 -0.1614050392102726 1105 52-53 -0.14015801466404554 1105 54-55 -0.17198119456190852 1105 56-57 -0.17320674206645492 1105 58-59 -0.09728432432235934 1105 60-61 -0.27854379337658486 1105 62-63 -0.17157146599195272 1105 64-65 -0.30926189508046065 1105 66-67 -0.2905302687405822 1105 68-69 -0.1756752495229179 1105 70-71 -0.4525120160442029 1105 72-73 -0.3523420268818356 1105 74-75 -0.5691622539095746 1105 76-77 -0.49779355174414874 1105 78-79 -0.5360487678520265 1105 80-81 -0.602776939936529 1105 82-83 -0.6963613898204777 1105 84-85 -0.7040355299765046 1105 86-87 -0.6175576133717335 1105 88-89 -0.9028320725108081 1105 90-91 -0.9956884544960154 1105 92-93 -0.8155269361826001 1105 94-95 -0.9143017161533038 1105 96-97 -1.0719689420798346 1105 98-99 -1.100342886971756 1105 100-101 -1.5091078526507218 1106 1 0.16293927854283652 1106 2 0.2231392903364764 1106 3 0.16943252177284052 1106 4 0.13700583063956628 1106 5 0.1827652324441047 1106 6 0.10172776357052271 1106 7 0.19111226668149328 1106 8 0.12395156668925722 1106 9 0.11413987322553965 1106 10-11 0.18395795215473498 1106 12-13 0.13725406798581474 1106 14-15 0.16479986822409387 1106 16-17 0.2496789674914126 1106 18-19 0.3141020956289111 1106 20-21 0.3601461947662372 1106 22-23 0.42645046381869633 1106 24-25 0.39127728504560366 1106 26-27 0.3015233047091428 1106 28-29 0.26597387416476437 1106 30-31 0.2807822807269815 1106 32-33 0.217631944827815 1106 34-35 0.2884074397502019 1106 36-37 0.2975083538005663 1106 38-39 0.3806770232918666 1106 40-41 0.37364232692348764 1106 42-43 0.41968903004694624 1106 44-45 0.366490489625491 1106 46-47 0.20789401304108424 1106 48-49 0.12066889806575176 1106 50-51 0.29250442458978654 1106 52-53 0.1557029630652167 1106 54-55 0.08304047113096402 1106 56-57 -0.036904027404077056 1106 58-59 0.2007347699597375 1106 60-61 0.042753538228630816 1106 62-63 0.02638549327772921 1106 64-65 -0.02925839535183883 1106 66-67 -0.30981377345187155 1106 68-69 -0.44820564229330984 1106 70-71 -0.8785365022810758 1106 72-73 -0.5973168021429061 1106 74-75 -0.16471448439339653 1106 76-77 -0.2750502093846805 1106 78-79 -0.38878300498533136 1106 80-81 -0.4756948238463785 1106 82-83 -0.35435678678757654 1106 84-85 -0.5059935984822097 1106 86-87 -0.8719696441337526 1106 88-89 -0.7756738818111906 1106 90-91 -0.8693139953968654 1106 92-93 -0.8065479078614928 1106 94-95 -0.8644185714988453 1106 96-97 -1.0182924475029331 1106 98-99 -1.165071257610812 1106 100-101 -1.56240708834558 1107 1 0.006272802428583191 1107 2 5.264356707996853E-4 1107 3 0.03271497761231501 1107 4 0.07101181398528666 1107 5 -0.0036521916683938116 1107 6 0.03572040824744249 1107 7 0.023946352696853523 1107 8 -0.0010298544506284202 1107 9 0.02262326658288316 1107 10-11 0.08536422895117113 1107 12-13 -0.032516697463215394 1107 14-15 0.08818255463199876 1107 16-17 -0.027943510071040123 1107 18-19 0.0023612087979856256 1107 20-21 0.09061743087973184 1107 22-23 0.09190426438654242 1107 24-25 0.08597230591109195 1107 26-27 0.05949315931764687 1107 28-29 0.007997350221856436 1107 30-31 0.048412189833477726 1107 32-33 0.015557781829279804 1107 34-35 0.04833140933804003 1107 36-37 0.08024525808608729 1107 38-39 -0.06305951894407258 1107 40-41 -0.1574205744275332 1107 42-43 -0.05141941333040734 1107 44-45 -0.1578106594055484 1107 46-47 -0.2039621579987596 1107 48-49 -0.5486502581681734 1107 50-51 -0.44923417561219736 1107 52-53 -0.4183295070656854 1107 54-55 -0.4636524917684781 1107 56-57 -0.5514679482104938 1107 58-59 -0.6364629662776125 1107 60-61 -0.7734872437984706 1107 62-63 -0.7896590197655868 1107 64-65 -0.7990139166079544 1107 66-67 -0.8266862367228924 1107 68-69 -0.6672875607749873 1107 70-71 -0.7289576231103609 1107 72-73 -0.8965765060244557 1107 74-75 -0.8295232650485183 1107 76-77 -0.7340080782465108 1107 78-79 -0.7098581016906813 1107 80-81 -0.6886304742970388 1107 82-83 -0.4652506681496469 1107 84-85 -0.644231750886032 1107 86-87 -0.8196182273765764 1107 88-89 -0.8592415946105945 1107 90-91 -1.074697101020071 1107 92-93 -1.1942877657093938 1107 94-95 -1.2610496048645707 1107 96-97 -1.5659076840737498 1107 98-99 -1.6179733708370527 1107 100-101 -2.263990578884652 1108 1 -0.05405532506124899 1108 2 -0.049347862896766514 1108 3 -0.06455778198517237 1108 4 -0.043467358031428205 1108 5 -0.015804866560635844 1108 6 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0.2934611089299466 2316 66-67 0.29416367063816296 2316 68-69 0.388761191014126 2316 70-71 0.5595307084814216 2316 72-73 0.7046216534901291 2316 74-75 0.771936127485926 2316 76-77 0.5805142824218947 2316 78-79 0.7334228679594581 2316 80-81 0.6879936340714927 2316 82-83 0.8665430733804058 2316 84-85 1.0405412178625966 2316 86-87 1.2261165764718207 2316 88-89 1.3766342604336153 2316 90-91 1.4519852542626879 2316 92-93 1.4238495623670815 2316 94-95 1.8717065278915612 2316 96-97 2.1698327981170387 2316 98-99 2.6936088378039784 2316 100-101 3.2640074541843767 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 5.0 4 14.0 5 31.0 6 77.0 7 196.0 8 454.0 9 873.0 10 1553.0 11 2600.0 12 4063.0 13 6184.0 14 8350.0 15 11307.0 16 15071.0 17 21238.0 18 21565.0 19 16923.0 20 15784.0 21 16441.0 22 17436.0 23 19519.0 24 21356.0 25 24684.0 26 28373.0 27 33740.0 28 39918.0 29 48470.0 30 58106.0 31 70949.0 32 88945.0 33 114508.0 34 154819.0 35 217608.0 36 306210.0 37 377603.0 38 203712.0 39 9823.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.776054527542044 18.711592713552978 14.20833916745817 23.30401359144681 2 15.825579784797956 19.91549028072513 40.17588414717369 24.08304578730322 3 19.73140025900581 23.669511821562992 29.7040337981352 26.895054121295995 4 12.712919388205348 16.331658164464415 36.500300479098634 34.4551219682316 5 13.181416130483347 37.8200041445749 34.43580710541878 14.56277261952297 6 32.9484405397496 35.841934663753314 16.807103255894823 14.402521540602272 7 29.077603387197126 32.888402327207004 19.838069980980627 18.195924304615243 8 26.732908467942273 35.34949803109311 19.05990824403629 18.85768525692832 9 26.52467085062539 16.355548546910832 19.906252637718605 37.21352796474517 10-11 25.237186184141695 26.57253517674168 26.855500783670937 21.33477785544569 12-13 26.0083679808986 23.86239230444975 27.530763297740606 22.598476416911044 14-15 22.49294797243783 25.458716639651374 25.992401348692372 26.05593403921842 16-17 21.847890009206672 28.718372124936785 27.00789731793446 22.42584054792208 18-19 21.807709745065605 26.88570029249298 29.65649890902695 21.650091053414467 20-21 22.308137087069102 26.026315776172865 30.059782391689904 21.60576474506813 22-23 22.00513113662864 26.171071246074696 29.882300257416066 21.941497359880596 24-25 21.77503365013063 26.270883172508537 30.15023905113455 21.803844126226277 26-27 21.39664027824118 26.67351653573559 29.870004145308222 22.059839040715016 28-29 21.45191141470644 27.084518550550214 29.74964926837294 21.713920766370403 30-31 21.675140527598302 26.46225277832956 29.830719906673803 22.031886787398335 32-33 21.28618065421992 26.556765726109916 29.79200498961592 22.36504863005425 34-35 22.026657622074197 26.78896916919487 29.652725054161404 21.531648154569524 36-37 22.061297555997697 26.264514493707726 29.573188529775006 22.10099942051957 38-39 21.57713176629357 26.687608828056092 29.63426191517161 22.10099749047873 40-41 22.141172040551485 26.447954166661404 29.643171689270385 21.76770210351673 42-43 21.677921211447288 26.481657450744517 29.55836863014668 22.282052707661514 44-45 21.737106512304596 26.82475207966001 29.579605040867907 21.85853636716749 46-47 22.1580379736119 27.017210717887817 28.806136753221057 22.018614555279225 48-49 21.95884602272483 27.086072681958246 28.817159898943466 22.137921396373457 50-51 22.197247198649833 27.349537838508585 28.580696424067476 21.872518538774102 52-53 22.38376391038215 27.145220965019295 28.576678300195596 21.894336824402963 54-55 21.90896936580829 27.297842388461063 28.68849973083562 22.104688514895027 56-57 22.006090489877998 27.508308200626235 28.334629146391155 22.150972163104612 58-59 21.941089961593157 27.119668883178388 28.444747888432598 22.494493266795853 60-61 21.713097469303914 27.418308879575097 28.686158910003297 22.182434741117696 62-63 21.795496585357963 27.30571119039037 28.854799481724626 22.043992742527042 64-65 21.557551149991696 27.35931493147773 28.611640735369836 22.47149318316074 66-67 21.487180524102232 27.50998362180524 28.500055604982204 22.502780249110323 68-69 22.074333686320973 27.10166473825364 28.54315874306738 22.280842832358 70-71 21.83871497718857 27.22456304422005 28.236379491197695 22.700342487393684 72-73 21.809152947253867 27.37582016992511 28.224333691703553 22.590693191117474 74-75 21.779123349205694 27.747726251929492 27.97300948626172 22.500140912603097 76-77 21.771182734816755 27.40200749715002 28.27540272536317 22.551407042670057 78-79 21.868170896787248 27.526880361379234 28.22104902608852 22.383899715745 80-81 21.941281047945402 27.541727094012902 27.9561040929175 22.56088776512419 82-83 21.89111161110423 27.664254059424465 28.048290908355472 22.396343421115834 84-85 21.885984535391913 27.69754785611191 27.913693432723395 22.50277417577279 86-87 22.001435880842113 27.670637247962464 27.765587396606467 22.562339474588956 88-89 22.02884497990831 27.767027888232715 27.485741686347183 22.718385445511792 90-91 21.909785155805647 27.716999208774762 27.685653544461548 22.687562090958043 92-93 21.918609148280773 27.454675429004187 27.82550248709436 22.801212935620683 94-95 21.781608396469036 27.362985523547138 27.877692613912814 22.977713466071013 96-97 22.041974868662663 27.03755799126229 27.78970355738718 23.130763582687862 98-99 21.751120416603904 27.16306370881401 27.57096814306555 23.51484773151654 100-101 22.173962383273885 26.645311582622515 27.654816987654275 23.525909046449332 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1302.0 1 850.5 2 574.0 3 781.5 4 627.5 5 527.0 6 1064.5 7 2238.0 8 3532.5 9 3984.5 10 3198.0 11 2300.5 12 2085.5 13 2155.0 14 2368.5 15 2540.0 16 2605.5 17 2828.0 18 3094.5 19 3408.5 20 3936.0 21 4614.0 22 5495.0 23 6798.5 24 8264.0 25 10136.5 26 13008.0 27 16005.5 28 18397.0 29 20871.5 30 23668.5 31 28204.0 32 34718.0 33 37880.5 34 39272.5 35 42802.5 36 47272.5 37 51299.0 38 53462.0 39 54989.5 40 57539.5 41 60572.0 42 61952.0 43 63459.5 44 65909.5 45 69302.0 46 71060.0 47 72379.0 48 73865.0 49 72377.5 50 70914.0 51 69225.0 52 67147.0 53 66493.0 54 66253.0 55 61918.0 56 55982.0 57 51310.0 58 47177.5 59 41855.0 60 33699.0 61 27649.0 62 23431.5 63 17210.0 64 11836.5 65 8926.5 66 6651.0 67 5116.5 68 3991.5 69 3102.0 70 2377.5 71 1592.5 72 1108.5 73 854.0 74 610.5 75 406.5 76 278.0 77 180.5 78 103.0 79 62.0 80 41.5 81 27.5 82 16.0 83 9.5 84 6.0 85 5.0 86 5.0 87 5.0 88 3.5 89 1.0 90 0.0 91 0.0 92 0.0 93 0.5 94 1.0 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.010411880865843926 2 0.013899355524791648 3 0.008845044424867412 4 1.0108622199848471E-4 5 9.603191089856049E-4 6 0.0018195519959727248 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 7.581466649886354E-4 14-15 0.0 16-17 0.0026535133274602236 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.003133672881953026 26-27 0.01865040795872043 28-29 0.007303479539390521 30-31 0.0036391039919454497 32-33 0.0 34-35 0.008238527092876506 36-37 6.317888874905295E-4 38-39 0.0022238968839666637 40-41 0.001844823551472346 42-43 0.0024766124389628756 44-45 2.527155549962118E-5 46-47 1.7690088849734828E-4 48-49 6.317888874905295E-4 50-51 0.004447793767933327 52-53 0.008870315980367034 54-55 0.008289070203875747 56-57 0.01733628707274013 58-59 0.024058520835639363 60-61 0.0040939919909386314 62-63 0.020722675509689367 64-65 0.021708266174174592 66-67 0.013596096858796194 68-69 0.03186743148502231 70-71 0.016881399073746948 72-73 0.005458655987918175 74-75 0.016805584407248084 76-77 0.02143027906367876 78-79 0.026863663496097312 80-81 0.012029260417819682 82-83 0.03558235014346662 84-85 0.022441141283663608 86-87 0.03179161681852344 88-89 0.022870757727157166 90-91 0.028506314603572693 92-93 0.025170469277622697 94-95 0.014986032411275359 96-97 0.015238747966271572 98-99 0.011473286196828015 100-101 0.010790954198338244 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1978509.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.29900309608925 #Duplication Level Percentage of deduplicated Percentage of total 1 79.93918882263719 47.40314205492426 2 12.082688650209043 14.32982783355657 3 3.5363269944586717 6.291019961795663 4 1.4650649686442208 3.4750756844642225 5 0.7831379001174961 2.3219648381866116 6 0.45266715417548464 1.6105626586169985 7 0.31262441199563445 1.2976821182389546 8 0.20700172297533057 0.9819996649287951 9 0.16772461630643726 0.8951312287481226 >10 0.9372734369798643 10.307792755962744 >50 0.07371934393025015 2.955721598736166 >100 0.037453223115329115 4.001920001341721 >500 0.0031627319139896186 1.3414161693918298 >1k 0.0017095848183727657 1.6073778774026715 >5k 2.5643772275591483E-4 1.1793655537046435 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9588 0.4846073482607357 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8424 0.4257751670576176 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5255 0.2656040483010186 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 2477 0.12519528594512333 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 2469 0.12479094105712939 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 2240 0.11321656863830289 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2192 0.11079049931033925 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 2090 0.10563510198841654 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 5.054311099924236E-5 7 0.0 0.0 0.0 0.0 5.054311099924236E-5 8 0.0 0.0 0.0 0.0 5.054311099924236E-5 9 0.0 0.0 0.0 0.0 5.054311099924236E-5 10-11 0.0 0.0 0.0 0.0 1.0108622199848471E-4 12-13 5.054311099924236E-5 0.0 0.0 0.0 1.7690088849734825E-4 14-15 5.054311099924236E-5 0.0 0.0 0.0 2.0217244399696943E-4 16-17 5.054311099924236E-5 0.0 0.0 0.0 2.0217244399696943E-4 18-19 5.054311099924236E-5 0.0 0.0 0.0 2.274439994965906E-4 20-21 5.054311099924236E-5 0.0 0.0 1.263577774981059E-4 2.527155549962118E-4 22-23 5.054311099924236E-5 0.0 0.0 2.527155549962118E-4 2.7798711049583296E-4 24-25 5.054311099924236E-5 0.0 0.0 4.548879989931812E-4 3.285302214950753E-4 26-27 5.054311099924236E-5 0.0 0.0 0.0014657502189780285 4.0434488799393885E-4 28-29 1.263577774981059E-4 0.0 0.0 0.004523608434432191 4.0434488799393885E-4 30-31 1.5162933299772707E-4 0.0 0.0 0.015516735076767403 4.0434488799393885E-4 32-33 1.7690088849734825E-4 0.0 0.0 0.03846330747042343 4.2961644349356003E-4 34-35 2.274439994965906E-4 0.0 0.0 0.07457636027938211 5.307026654920448E-4 36-37 2.527155549962118E-4 0.0 0.0 0.1329031103725078 5.559742209916659E-4 38-39 2.527155549962118E-4 0.0 0.0 0.2180935239617308 6.317888874905295E-4 40-41 2.527155549962118E-4 0.0 0.0 0.30530566199092346 7.581466649886354E-4 42-43 2.7798711049583296E-4 0.0 0.0 0.3893588555826635 7.581466649886354E-4 44-45 3.0325866599545414E-4 0.0 0.0 0.4947412420160838 8.086897759878777E-4 46-47 4.548879989931812E-4 0.0 0.0 0.6198859848502078 8.339613314874989E-4 48-49 5.559742209916659E-4 0.0 0.0 0.7507926423382456 8.845044424867412E-4 50-51 5.559742209916659E-4 0.0 0.0 0.8922375384696253 9.097759979863624E-4 52-53 5.559742209916659E-4 0.0 0.0 1.0238265279561527 9.097759979863624E-4 54-55 5.559742209916659E-4 0.0 0.0 1.1613796045405909 9.097759979863624E-4 56-57 5.559742209916659E-4 0.0 0.0 1.3210452921871976 9.097759979863624E-4 58-59 5.559742209916659E-4 0.0 0.0 1.4946103353585958 9.097759979863624E-4 60-61 5.559742209916659E-4 0.0 0.0 1.674720711404396 9.097759979863624E-4 62-63 5.559742209916659E-4 0.0 0.0 1.8481088536872967 9.097759979863624E-4 64-65 5.559742209916659E-4 0.0 0.0 2.0360534119379796 9.097759979863624E-4 66-67 5.559742209916659E-4 0.0 0.0 2.239034545710937 9.097759979863624E-4 68-69 5.559742209916659E-4 0.0 0.0 2.461045160775109 9.097759979863624E-4 70-71 5.559742209916659E-4 0.0 0.0 2.6969551313640725 9.097759979863624E-4 72-73 5.559742209916659E-4 0.0 0.0 2.933143089063532 9.097759979863624E-4 74-75 5.559742209916659E-4 0.0 0.0 3.163771304553075 9.603191089856048E-4 76-77 5.559742209916659E-4 0.0 0.0 3.410649130228874 0.0010108622199848471 78-79 5.559742209916659E-4 0.0 0.0 3.690076719388186 0.0010614053309840896 80-81 6.570604429901506E-4 0.0 0.0 3.9858297334002524 0.0010614053309840896 82-83 6.570604429901506E-4 0.0 0.0 4.285651467847758 0.0011119484419833319 84-85 7.07603553989393E-4 0.0 0.0 4.58173301208132 0.0011119484419833319 86-87 7.834182204882565E-4 0.0 0.0 4.888024264736728 0.001137219997482953 88-89 8.086897759878777E-4 5.054311099924236E-5 0.0 5.209655351580407 0.0011624915529825743 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4750 0.0 49.808613 1 GTATCAA 7970 0.0 38.03042 1 ATCAACG 9885 0.0 30.318007 3 TCAACGC 10035 0.0 29.909885 4 TATCAAC 10135 0.0 29.669127 2 CAACGCA 10190 0.0 29.408321 5 ACCGCGC 130 3.0449155E-7 29.224005 8 AACGCAG 10440 0.0 28.839838 6 ACGCAGA 12010 0.0 25.029593 7 CGCAGAG 11985 0.0 25.002556 8 GTACATG 7965 0.0 24.93212 1 TACATGG 7970 0.0 24.49674 2 TGGTATC 2250 0.0 24.279522 2 GAGTACT 8240 0.0 22.796452 12-13 ACATGGG 8005 0.0 22.664701 3 GCAGAGT 13240 0.0 22.345657 9 GTACTTT 8675 0.0 21.625969 14-15 TAAGGTG 1025 0.0 21.313217 5 GTGGTAT 2570 0.0 21.25854 1 GTAAGGT 1010 0.0 21.159538 4 >>END_MODULE