FastQCFastQC Report
Thu 26 May 2016
SRR937935_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937935_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1636733
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT235741.4403082237603813No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT177251.082949998564213No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT126450.7725756125159082No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA52490.32069983314321887No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38340.23424712521834656No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37340.22813739320952167No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25770.15744779386741758No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24690.15084928329788672No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20450.12494401958046913No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19330.11810111973058525No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA87750.044.7792551
GTATCAA150400.039.7283021
TATCAAC186450.032.01262
ATCAACG188150.031.672843
TCAACGC191050.031.2418174
CAACGCA193200.030.8695535
AACGCAG198000.030.069526
ACGCAGA218500.027.08957
CGCAGAG219700.026.8982938
GTACATG127450.026.1911741
GCAGAGT227450.025.604279
TACATGG129600.025.3376772
GAGTACT129400.024.63179812-13
ACATGGG130300.024.2533073
AGTACTT129450.023.87003712-13
GTACTTT137350.022.94704414-15
CATGGGA84450.022.2838024
GTATTCG1302.865241E-422.009221
CAGAGTA224400.021.33759910-11
AGAGTAC210800.021.12557410-11
Produced by FastQC (version 0.11.5)