FastQCFastQC Report
Thu 26 May 2016
SRR937935_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937935_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1636733
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT222641.360270734444775No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT181731.1103215979637486No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT128110.7827177676505574No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA52430.32033324922268935No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39900.24377830715211338No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39590.24188429022937766No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22900.13991286300209016No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22770.1391185978409429No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19660.12011733129349747No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19280.117795633130144No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA90550.047.732591
GTATCAA147500.037.4587821
TATCAAC185950.029.4830672
ATCAACG187900.029.098513
TCAACGC190900.028.6403524
CAACGCA194650.028.0885855
AACGCAG198300.027.5908366
GTCGACG458.950153E-426.38936424-25
GTACATG129800.024.2872521
ACGCAGA224000.024.2768637
CGCAGAG225300.024.0735448
GAGTACT132700.023.83627512-13
TACATGG131200.023.303262
GCAGAGT230000.023.1892669
GTACTTT140750.022.48986414-15
GTGGTAT44700.022.1256161
ACATGGG135600.021.9840413
AGTACTT133950.021.84102812-13
TGGTATC45550.021.6083432
CAGAGTA227900.020.6312710-11
Produced by FastQC (version 0.11.5)