##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937935_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1636733 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.16104886991342 33.0 31.0 34.0 31.0 34.0 2 32.40458462070478 34.0 31.0 34.0 31.0 34.0 3 32.447276373116445 34.0 31.0 34.0 30.0 34.0 4 36.02964075386761 37.0 35.0 37.0 35.0 37.0 5 35.957807412693455 37.0 35.0 37.0 35.0 37.0 6 36.03002016822536 37.0 35.0 37.0 35.0 37.0 7 35.9898248523125 37.0 35.0 37.0 35.0 37.0 8 35.994732800035194 37.0 35.0 37.0 35.0 37.0 9 37.59140922801703 39.0 37.0 39.0 35.0 39.0 10-11 37.55218535949358 39.0 37.0 39.0 35.0 39.0 12-13 37.44180235872314 39.0 37.0 39.0 34.5 39.0 14-15 38.46601583764732 40.0 38.0 41.0 34.0 41.0 16-17 38.362436634441906 40.0 38.0 41.0 34.0 41.0 18-19 38.72073331447463 40.0 38.0 41.0 35.0 41.0 20-21 38.72368309308849 40.0 38.0 41.0 34.0 41.0 22-23 38.57448618681239 40.0 38.0 41.0 34.0 41.0 24-25 38.42089546676215 40.0 38.0 41.0 34.0 41.0 26-27 38.20075632372537 40.0 38.0 41.0 34.0 41.0 28-29 38.02893141398139 40.0 37.0 41.0 33.5 41.0 30-31 37.67310367665343 39.5 37.0 41.0 33.0 41.0 32-33 37.317813901228845 39.0 35.5 41.0 33.0 41.0 34-35 37.11823919967399 39.0 35.0 41.0 32.0 41.0 36-37 37.004115515481146 39.0 35.0 41.0 32.0 41.0 38-39 36.756315477234224 39.0 35.0 40.0 31.0 41.0 40-41 36.452239002940615 39.0 35.0 40.0 30.5 41.0 42-43 36.07435238368139 38.0 35.0 40.0 30.0 41.0 44-45 35.633109676410264 38.0 35.0 40.0 29.0 41.0 46-47 35.02546780690558 38.0 34.5 40.0 26.5 41.0 48-49 34.401289031259225 38.0 33.5 40.0 24.0 41.0 50-51 33.9057051455552 38.0 33.0 40.0 22.5 41.0 52-53 33.27013782944439 37.0 33.0 40.0 19.5 41.0 54-55 32.80620999271109 37.0 32.0 39.5 17.5 41.0 56-57 32.34960589173677 36.0 31.0 39.0 13.5 40.0 58-59 31.757544449827797 35.5 30.5 39.0 8.5 40.0 60-61 31.18702531200874 35.0 29.5 38.5 7.5 40.0 62-63 30.52464085467819 35.0 28.5 38.0 2.0 40.0 64-65 29.856457345211467 34.0 28.0 38.0 2.0 40.0 66-67 29.175101864506917 34.0 26.5 37.5 2.0 39.5 68-69 28.458672245259308 33.5 26.0 36.5 2.0 39.0 70-71 27.756005102848174 33.0 25.5 36.0 2.0 39.0 72-73 26.977189315545054 32.5 23.0 35.5 2.0 38.5 74-75 26.224569615202967 32.0 21.5 35.0 2.0 38.0 76-77 25.077011950024836 30.5 20.5 34.0 2.0 36.0 78-79 25.696110483505862 31.5 20.5 35.0 2.0 36.0 80-81 25.321526174397412 31.5 19.0 35.0 2.0 36.0 82-83 24.65773403481203 31.0 15.5 34.5 2.0 36.0 84-85 23.864440321054197 31.0 7.0 34.0 2.0 35.0 86-87 22.989105125881864 30.0 2.0 34.0 2.0 35.0 88-89 22.037340849118337 29.0 2.0 34.0 2.0 35.0 90-91 21.220517029961513 29.0 2.0 34.0 2.0 35.0 92-93 20.121147737596786 27.0 2.0 33.5 2.0 35.0 94-95 19.065802119221644 25.5 2.0 33.0 2.0 35.0 96-97 17.879531053629393 24.0 2.0 32.5 2.0 34.5 98-99 16.420530410274615 10.0 2.0 32.0 2.0 34.0 100-101 13.251525752825904 2.0 2.0 28.0 2.0 32.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.12836207223165275 1101 2 0.1475048276899571 1101 3 0.1670614442247782 1101 4 0.1176993184456947 1101 5 0.07651997513904263 1101 6 0.06736132885500012 1101 7 0.14925860829198 1101 8 0.0768987991572061 1101 9 0.12734037010236676 1101 10-11 0.15437080298550399 1101 12-13 0.29606174516826655 1101 14-15 0.43534461362553145 1101 16-17 0.48757998792083157 1101 18-19 0.4119692793810614 1101 20-21 0.16826042384734308 1101 22-23 0.31200019191417283 1101 24-25 0.34976233164110937 1101 26-27 0.3879747062643304 1101 28-29 0.3980719771166221 1101 30-31 0.42483569083432826 1101 32-33 0.4714425226598493 1101 34-35 0.4898540678135319 1101 36-37 0.5946919720507466 1101 38-39 0.6790264568590771 1101 40-41 0.9238249259346034 1101 42-43 0.9656965933514101 1101 44-45 0.8812016624283174 1101 46-47 1.0022672511198323 1101 48-49 1.1830487530002003 1101 50-51 1.150221840208495 1101 52-53 1.3012848700032507 1101 54-55 1.387434281230611 1101 56-57 1.3884339030667476 1101 58-59 1.7471396159945378 1101 60-61 1.6817050248669254 1101 62-63 1.8748597684555754 1101 64-65 2.051093113904358 1101 66-67 2.2112161380113164 1101 68-69 2.246293205056073 1101 70-71 2.4832218633250456 1101 72-73 2.4676219926742355 1101 74-75 2.5229950307490014 1101 76-77 2.6502852332162625 1101 78-79 2.639741430816173 1101 80-81 2.9321437530043184 1101 82-83 3.23338210582644 1101 84-85 3.317340909678599 1101 86-87 3.5987990662126776 1101 88-89 4.037000011938311 1101 90-91 4.4004212775704765 1101 92-93 4.669688200208913 1101 94-95 4.740351218180841 1101 96-97 4.621696849867842 1101 98-99 4.906931802020988 1101 100-101 4.4624563167705364 1102 1 0.2540223397165562 1102 2 0.22165914712525137 1102 3 0.2623011692499375 1102 4 0.15891131880878362 1102 5 0.03166518810367336 1102 6 0.03504796586946668 1102 7 0.04397294665547946 1102 8 0.11041749596813588 1102 9 0.13328271546374282 1102 10-11 0.25886208514820197 1102 12-13 0.3996482829286876 1102 14-15 0.5676696827338077 1102 16-17 0.589418047601221 1102 18-19 0.49621144929940186 1102 20-21 0.41981508127212663 1102 22-23 0.4735994962927137 1102 24-25 0.46261481118143166 1102 26-27 0.5752476211236086 1102 28-29 0.4341389877250421 1102 30-31 0.6165408116182007 1102 32-33 0.6988719027462551 1102 34-35 0.7012138182945478 1102 36-37 0.8231151325154045 1102 38-39 0.9806203248348453 1102 40-41 1.129344759628303 1102 42-43 1.144503402148274 1102 44-45 1.0032789139100586 1102 46-47 1.2098934428750496 1102 48-49 1.1942318203912592 1102 50-51 1.385050134700819 1102 52-53 1.538292189802128 1102 54-55 1.6252749182420985 1102 56-57 1.724255531517393 1102 58-59 1.8332597257741519 1102 60-61 1.864180413487876 1102 62-63 2.210968097709131 1102 64-65 2.2674277185424607 1102 66-67 2.373432493944911 1102 68-69 2.470225876993471 1102 70-71 2.5077256055641612 1102 72-73 2.740158387059463 1102 74-75 2.792627711465993 1102 76-77 2.7164198665244434 1102 78-79 3.0942455351514546 1102 80-81 3.514003528534456 1102 82-83 3.4182552655222587 1102 84-85 3.50676284050434 1102 86-87 3.761388926825493 1102 88-89 4.073969534849326 1102 90-91 4.1164520686414825 1102 92-93 4.145363025943933 1102 94-95 4.450588470535578 1102 96-97 4.534016750425323 1102 98-99 4.650382945575252 1102 100-101 4.115485102423987 1103 1 0.30993684561138224 1103 2 0.25563639672551375 1103 3 0.2486052190658654 1103 4 0.2086336029722844 1103 5 0.048509867044138844 1103 6 0.10203540850553594 1103 7 0.15713061813532647 1103 8 0.17954396017104557 1103 9 0.3209431478761786 1103 10-11 0.27479441158681794 1103 12-13 0.36321143668281053 1103 14-15 0.5410948663517061 1103 16-17 0.49192335459068914 1103 18-19 0.4826128538030261 1103 20-21 0.3865944166734039 1103 22-23 0.5467717431600931 1103 24-25 0.5616699607576336 1103 26-27 0.6856047227540287 1103 28-29 0.6253505482697008 1103 30-31 0.7579461775304352 1103 32-33 0.764331572176097 1103 34-35 0.8759601513838788 1103 36-37 0.7877626320529671 1103 38-39 0.7591741070412752 1103 40-41 0.9059150559713345 1103 42-43 0.9982743432574921 1103 44-45 0.8997329316684741 1103 46-47 1.0232612614022045 1103 48-49 1.09207685119938 1103 50-51 1.296300906004923 1103 52-53 1.2317867177732893 1103 54-55 1.511477719182821 1103 56-57 1.5258981368957265 1103 58-59 1.7380955185495957 1103 60-61 1.8528374333867141 1103 62-63 1.9343322028503387 1103 64-65 2.054058926468432 1103 66-67 2.213921010495902 1103 68-69 2.293929054604117 1103 70-71 2.4425906835568547 1103 72-73 2.9216198756311833 1103 74-75 3.12215130861971 1103 76-77 3.0553173293222073 1103 78-79 3.238124173724959 1103 80-81 3.4193462610765586 1103 82-83 3.557232790960807 1103 84-85 3.7915106988171132 1103 86-87 3.744382917259699 1103 88-89 3.857626325269255 1103 90-91 4.217564183690616 1103 92-93 4.417355333996415 1103 94-95 4.525525155219043 1103 96-97 4.785879586015042 1103 98-99 4.852333423563589 1103 100-101 4.154416314846818 1104 1 0.2995373772399361 1104 2 0.16891534802981312 1104 3 0.24207405242795943 1104 4 0.14476906178952476 1104 5 0.13557735004224014 1104 6 0.1741259399022681 1104 7 0.20770830913996718 1104 8 0.22545788133513156 1104 9 0.26214204237727046 1104 10-11 0.3170076674425033 1104 12-13 0.3240512672691338 1104 14-15 0.5654860735314458 1104 16-17 0.5466614768738438 1104 18-19 0.42404077271594076 1104 20-21 0.4848971890922513 1104 22-23 0.49396412280744073 1104 24-25 0.5391444332287776 1104 26-27 0.6636100081308953 1104 28-29 0.6214728022305067 1104 30-31 0.5248008288080328 1104 32-33 0.6399032746323812 1104 34-35 0.7646109292941645 1104 36-37 0.7618030262067705 1104 38-39 0.9448094469461239 1104 40-41 0.8736773479497728 1104 42-43 0.9307250203712698 1104 44-45 1.1895211474073264 1104 46-47 1.3639304591427859 1104 48-49 1.4859790923331104 1104 50-51 1.5189890473054675 1104 52-53 1.6411241739753208 1104 54-55 1.7975247020276655 1104 56-57 1.7251259790521871 1104 58-59 1.9750456345857792 1104 60-61 2.169115423947556 1104 62-63 2.466380166185825 1104 64-65 2.545483496945703 1104 66-67 2.5164249105648437 1104 68-69 2.783118115007319 1104 70-71 2.893308150284998 1104 72-73 3.294359788898113 1104 74-75 3.2795692097000533 1104 76-77 3.2779636151772955 1104 78-79 3.637011720377682 1104 80-81 3.5845741736360246 1104 82-83 3.535936954955165 1104 84-85 3.697708352679875 1104 86-87 3.8846559693072713 1104 88-89 4.132347653859828 1104 90-91 4.390317490700095 1104 92-93 4.715154896639209 1104 94-95 4.747917518038356 1104 96-97 4.98317885803079 1104 98-99 5.313250096454912 1104 100-101 4.5278205491583225 1105 1 0.22570055340310802 1105 2 0.24375642287088795 1105 3 0.25586300371691095 1105 4 0.14078618280665012 1105 5 0.0869711139360092 1105 6 0.022632725909055296 1105 7 0.1773419037735664 1105 8 0.10447244784970167 1105 9 0.2162365589717936 1105 10-11 0.16466985885469398 1105 12-13 0.23043289240076348 1105 14-15 0.4271639039592685 1105 16-17 0.4483094722718377 1105 18-19 0.27471815464332394 1105 20-21 0.2615061844512496 1105 22-23 0.3100825951759063 1105 24-25 0.3197289950633362 1105 26-27 0.4575252707961539 1105 28-29 0.30372851573621773 1105 30-31 0.49117426393146246 1105 32-33 0.5973408258199342 1105 34-35 0.7338788985621321 1105 36-37 0.6384398090935548 1105 38-39 0.6169056502923382 1105 40-41 0.6886770066994288 1105 42-43 0.7380575152037707 1105 44-45 0.8194485585847389 1105 46-47 0.8834999474622762 1105 48-49 1.0942066380606548 1105 50-51 1.156119278185706 1105 52-53 1.3539961368472788 1105 54-55 1.391458733461704 1105 56-57 1.3995609659871775 1105 58-59 1.4386951607353033 1105 60-61 1.497145970728102 1105 62-63 1.8137596073152658 1105 64-65 1.7957918410040499 1105 66-67 1.8852104981004345 1105 68-69 2.1896703653095706 1105 70-71 2.4669280364048802 1105 72-73 2.3806156876540108 1105 74-75 2.368975580356423 1105 76-77 2.2883217817854664 1105 78-79 2.329596350462314 1105 80-81 2.6459297662416184 1105 82-83 2.928697816466027 1105 84-85 3.085296834018358 1105 86-87 3.1129110100623087 1105 88-89 3.279664207426382 1105 90-91 3.394463682346288 1105 92-93 3.6411425726268867 1105 94-95 3.414107652978494 1105 96-97 3.317172754524691 1105 98-99 3.128733599438416 1105 100-101 2.7205660544038306 1106 1 0.15139003246337523 1106 2 0.0303867712989927 1106 3 0.02368873291904805 1106 4 0.09265032989551258 1106 5 -0.019096585530569143 1106 6 0.028342917714752502 1106 7 0.027458235631470984 1106 8 0.16639078856017875 1106 9 0.14906909784365752 1106 10-11 0.1193891018464015 1106 12-13 0.12444676710283176 1106 14-15 -0.02912298175176886 1106 16-17 -0.12466394164258787 1106 18-19 -0.1138763578978157 1106 20-21 -0.15378081475429894 1106 22-23 -0.025779537385659523 1106 24-25 0.012623530303379482 1106 26-27 -0.16741443525310018 1106 28-29 0.0885819659688849 1106 30-31 0.1446365553853326 1106 32-33 0.23960972265343372 1106 34-35 0.2563791822983674 1106 36-37 0.30115899533802093 1106 38-39 0.19590845928109957 1106 40-41 0.21302512269080154 1106 42-43 0.27117662767681594 1106 44-45 0.3108619906582817 1106 46-47 0.5990721015878009 1106 48-49 0.6578841434516391 1106 50-51 0.8508510055719185 1106 52-53 0.7832606925163361 1106 54-55 1.017728453130296 1106 56-57 0.9251185076964035 1106 58-59 1.168911651485498 1106 60-61 0.9829485186795317 1106 62-63 1.0332331347241883 1106 64-65 1.1695043878205276 1106 66-67 1.4353076777622178 1106 68-69 1.746704896009831 1106 70-71 1.7983532435323362 1106 72-73 1.9193953380909683 1106 74-75 2.058153170868284 1106 76-77 2.1290570842482985 1106 78-79 2.273707404826176 1106 80-81 2.091065817768115 1106 82-83 2.553672989545131 1106 84-85 2.8719307291494403 1106 86-87 3.315236228539213 1106 88-89 3.346054940600819 1106 90-91 3.20374327603599 1106 92-93 2.9127349003989877 1106 94-95 3.5977878538637498 1106 96-97 3.77298792873874 1106 98-99 3.8138502150325557 1106 100-101 3.3040341624505896 1107 1 0.206233813366552 1107 2 0.21524336492765173 1107 3 0.2606545794618427 1107 4 0.12810694838351822 1107 5 0.12224677284191188 1107 6 0.13433245646445613 1107 7 0.24856999558713255 1107 8 0.2833672980422932 1107 9 0.22591339938595212 1107 10-11 0.3059237012865239 1107 12-13 0.40803092184991385 1107 14-15 0.5443234850551377 1107 16-17 0.523285318713711 1107 18-19 0.4196646056910609 1107 20-21 0.31075592196413027 1107 22-23 0.47126450665134456 1107 24-25 0.47643676626312725 1107 26-27 0.4144586069757352 1107 28-29 0.5642467775655362 1107 30-31 0.6343943878380358 1107 32-33 0.7297902812382304 1107 34-35 0.7278290449856613 1107 36-37 0.8287720213222869 1107 38-39 0.8999298101754505 1107 40-41 0.9243297536428727 1107 42-43 1.0299846924236817 1107 44-45 1.1617631810144147 1107 46-47 1.1653480543925596 1107 48-49 1.3300532854993463 1107 50-51 1.3017215517036007 1107 52-53 1.3765162661300963 1107 54-55 1.552044316910461 1107 56-57 1.551939083416947 1107 58-59 1.6193285927384942 1107 60-61 1.613799552570324 1107 62-63 1.72445172732564 1107 64-65 1.931112842186824 1107 66-67 2.2353922229886365 1107 68-69 2.5210660772821463 1107 70-71 2.692168465112747 1107 72-73 2.8011133221075006 1107 74-75 2.6903529024186277 1107 76-77 2.7328063503894775 1107 78-79 2.804081733408875 1107 80-81 2.8811939215707376 1107 82-83 3.0752132605119975 1107 84-85 3.310804295926097 1107 86-87 3.557759858597759 1107 88-89 3.654869571967218 1107 90-91 3.802609400643849 1107 92-93 3.8306187368995417 1107 94-95 3.7234141256576976 1107 96-97 3.81814008278538 1107 98-99 3.760603725242479 1107 100-101 3.5084776531717186 1108 1 0.18169524116363078 1108 2 0.26473395248565623 1108 3 0.16891506948011425 1108 4 0.15334701182621302 1108 5 0.039673521892730435 1108 6 0.12883510478418003 1108 7 0.182225189606946 1108 8 0.19219346803233606 1108 9 0.22073814876608822 1108 10-11 0.16256689543934755 1108 12-13 0.28087311516583924 1108 14-15 0.38955472677476166 1108 16-17 0.3970537528899172 1108 18-19 0.3591923350268118 1108 20-21 0.40114453048354903 1108 22-23 0.3377385937412214 1108 24-25 0.41520571524015537 1108 26-27 0.453481110382036 1108 28-29 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-0.24207925133321595 2311 2 -0.17535103971483323 2311 3 -0.2931915045767255 2311 4 -0.1509227121631156 2311 5 -0.15089880599913386 2311 6 -0.15515054154812447 2311 7 -0.24201470580043605 2311 8 -0.10906130101013645 2311 9 -0.19867974453146786 2311 10-11 -0.16985114550326585 2311 12-13 -0.29597774406422417 2311 14-15 -0.32404488121690633 2311 16-17 -0.22190965039293786 2311 18-19 -0.2633707190433725 2311 20-21 -0.25524261626495104 2311 22-23 -0.2505435238741214 2311 24-25 -0.2804946038015643 2311 26-27 -0.35534528256352615 2311 28-29 -0.18396510103348618 2311 30-31 -0.3768802359165093 2311 32-33 -0.3555545577819714 2311 34-35 -0.1612175508821423 2311 36-37 -0.16977929667989855 2311 38-39 -0.2542969695515893 2311 40-41 -0.43586798333469545 2311 42-43 -0.31201891802483317 2311 44-45 -0.0981376293818883 2311 46-47 -0.23669130452245923 2311 48-49 -0.3994950105322985 2311 50-51 -0.3114377722517858 2311 52-53 -0.15819939708738673 2311 54-55 -0.23517761328334075 2311 56-57 -0.11604450342264272 2311 58-59 0.2826636922038368 2311 60-61 0.32173698961259944 2311 62-63 0.2428530078760609 2311 64-65 0.36873173323742137 2311 66-67 0.5845692613178919 2311 68-69 1.0890434861500822 2311 70-71 1.7073612143380608 2311 72-73 1.8671216333990905 2311 74-75 1.8848967063319009 2311 76-77 1.8895905311520416 2311 78-79 1.838200367218505 2311 80-81 1.6907603885178908 2311 82-83 1.6508007437319954 2311 84-85 2.262101460551243 2311 86-87 2.478272399880222 2311 88-89 2.5915423956632644 2311 90-91 2.5101840127405275 2311 92-93 2.639689919000322 2311 94-95 2.534406587136125 2311 96-97 2.62803429636449 2311 98-99 2.480087861118765 2311 100-101 2.388172300814423 2312 1 -0.1564442314134311 2312 2 -0.12654969171003216 2312 3 -0.18130516102758776 2312 4 -0.12161564352647503 2312 5 -0.061766122110391564 2312 6 -0.14140060081511052 2312 7 -0.13740452452248064 2312 8 -0.2112825668556937 2312 9 -0.31964639818979634 2312 10-11 -0.26750459836747353 2312 12-13 -0.20151437470872224 2312 14-15 -0.28863331303069373 2312 16-17 -0.1948158237637685 2312 18-19 -0.16988084792323832 2312 20-21 -0.21863567856275523 2312 22-23 -0.24822299342547893 2312 24-25 -0.2676555510521226 2312 26-27 -0.21957252008979822 2312 28-29 -0.270492772941175 2312 30-31 -0.34513835792505176 2312 32-33 -0.3011599259728044 2312 34-35 -0.355622990558075 2312 36-37 -0.24445022813808492 2312 38-39 -0.17390905345175867 2312 40-41 -0.11071653339067922 2312 42-43 0.002482710449569936 2312 44-45 -0.04538606078232732 2312 46-47 0.12000932972546963 2312 48-49 0.2919783849736248 2312 50-51 0.39094469979547597 2312 52-53 0.3986300440766115 2312 54-55 0.4685828921698274 2312 56-57 0.5185720969257872 2312 58-59 0.7136468312022366 2312 60-61 0.8608294935242249 2312 62-63 1.0692469369915294 2312 64-65 1.2938796575556069 2312 66-67 1.29920905785281 2312 68-69 1.6815015038487253 2312 70-71 1.8222774685672896 2312 72-73 1.7470354743982277 2312 74-75 2.1697639300474485 2312 76-77 2.342741042550145 2312 78-79 2.206716103059165 2312 80-81 2.08707203682091 2312 82-83 2.3222382507984456 2312 84-85 2.4593994330732443 2312 86-87 2.699099365811737 2312 88-89 2.8389604826100587 2312 90-91 2.9047471329923304 2312 92-93 3.106449953964802 2312 94-95 3.1969114956582914 2312 96-97 3.2863953788045634 2312 98-99 2.630102936208516 2312 100-101 2.659254327538667 2313 1 -0.192485241554337 2313 2 -0.28000083474463366 2313 3 -0.2873496344760369 2313 4 -0.19964768341461792 2313 5 -0.15576629956912313 2313 6 -0.15227481405754162 2313 7 -0.24025731677841122 2313 8 -0.22897720617081774 2313 9 -0.3183603948180789 2313 10-11 -0.3161924055213561 2313 12-13 -0.2854031252561242 2313 14-15 -0.2333313040260947 2313 16-17 -0.0688965445896983 2313 18-19 -0.2664926741167122 2313 20-21 -0.18875677983731265 2313 22-23 -0.275085523013864 2313 24-25 -0.37263112185386404 2313 26-27 -0.24286097353693492 2313 28-29 -0.2789163383161153 2313 30-31 -0.2580308780394276 2313 32-33 -0.14138352397725384 2313 34-35 -0.10239724873764544 2313 36-37 -0.07539688461518779 2313 38-39 -0.17664749931396528 2313 40-41 -0.04670287479265767 2313 42-43 0.04751329345876343 2313 44-45 0.17239186964813058 2313 46-47 0.2609789699708216 2313 48-49 0.3481625356189184 2313 50-51 0.31681431914649494 2313 52-53 0.44257483723700375 2313 54-55 0.5249580971776027 2313 56-57 0.6104901396131979 2313 58-59 0.9458980955957195 2313 60-61 0.8111755380549148 2313 62-63 1.031767938660213 2313 64-65 1.0679320422288967 2313 66-67 1.2560204678769367 2313 68-69 1.3674496343782074 2313 70-71 1.7898869563126176 2313 72-73 1.957201426936603 2313 74-75 2.0997006459732255 2313 76-77 2.322775074555686 2313 78-79 2.381595674410459 2313 80-81 2.3931783346023927 2313 82-83 2.414655324618419 2313 84-85 2.5340337106587754 2313 86-87 2.6450523049004566 2313 88-89 2.7004254617014176 2313 90-91 2.9007062258919767 2313 92-93 2.9910312229092355 2313 94-95 3.2911293462895586 2313 96-97 3.3852481939583967 2313 98-99 3.5764220986032917 2313 100-101 3.4265680030238865 2314 1 -0.10121437458680305 2314 2 -0.15533676384684014 2314 3 -0.16745016202546026 2314 4 -0.08620515161782549 2314 5 0.009091174001795821 2314 6 -0.08816620466570413 2314 7 -0.08845383547524222 2314 8 -0.13486806000810958 2314 9 -0.22478581738750592 2314 10-11 -0.07785320211684876 2314 12-13 -0.1927622538705549 2314 14-15 -0.09935604778682006 2314 16-17 0.02202032887138472 2314 18-19 -0.030478376742905766 2314 20-21 0.005487522477551465 2314 22-23 -0.018983332851085777 2314 24-25 0.04707869155631528 2314 26-27 -0.032818816378679116 2314 28-29 -0.023551726380809157 2314 30-31 -0.02198052067389966 2314 32-33 0.12323873384618622 2314 34-35 0.19464280400353573 2314 36-37 0.17951339895122942 2314 38-39 0.2217878824195907 2314 40-41 0.31545656817681333 2314 42-43 0.4847029814022008 2314 44-45 0.48771502635631947 2314 46-47 0.5367205051630322 2314 48-49 0.6200229421609151 2314 50-51 0.659736169436961 2314 52-53 0.6945463691122455 2314 54-55 1.0382064280266476 2314 56-57 1.2911489088664112 2314 58-59 1.3452267191087621 2314 60-61 1.3856149221722767 2314 62-63 1.6646218943174382 2314 64-65 1.8658280380558487 2314 66-67 2.064654220854983 2314 68-69 2.3261788362274523 2314 70-71 2.6005045593691882 2314 72-73 2.7414609652861266 2314 74-75 2.9383308067154523 2314 76-77 2.834908023065598 2314 78-79 2.911108575491845 2314 80-81 3.1120886346848344 2314 82-83 3.376489677128234 2314 84-85 3.6400239359236153 2314 86-87 3.821044057897769 2314 88-89 3.8582232972761084 2314 90-91 4.168745236747139 2314 92-93 4.253589803191577 2314 94-95 4.59142470790108 2314 96-97 4.661510534527125 2314 98-99 4.375262535892627 2314 100-101 4.053760677615495 2315 1 -0.30974650982172847 2315 2 -0.31661581805690275 2315 3 -0.32945779688166255 2315 4 -0.21172036414434103 2315 5 -0.16426813035879206 2315 6 -0.28037419515342066 2315 7 -0.3608642316547943 2315 8 -0.39816971451468675 2315 9 -0.44993908637052016 2315 10-11 -0.3838360334012023 2315 12-13 -0.43124172413497064 2315 14-15 -0.3989327103317848 2315 16-17 -0.34004864594739814 2315 18-19 -0.5102963421767299 2315 20-21 -0.4346334526606199 2315 22-23 -0.350165721322405 2315 24-25 -0.2515976977911336 2315 26-27 -0.3375200902214246 2315 28-29 -0.26458190326864184 2315 30-31 -0.3223896156046351 2315 32-33 -0.28959566875544596 2315 34-35 -0.3952830917109722 2315 36-37 -0.2506945904575133 2315 38-39 -0.2198999052632189 2315 40-41 -0.3488159787102276 2315 42-43 -0.11835382037423159 2315 44-45 -0.21095969057351027 2315 46-47 -0.24745076334955485 2315 48-49 -0.09009003234712054 2315 50-51 -0.20797303382327925 2315 52-53 -0.022476927125779866 2315 54-55 0.181675718123131 2315 56-57 0.3274743591783462 2315 58-59 0.5954011091079217 2315 60-61 0.7240562774995212 2315 62-63 0.852304781277109 2315 64-65 1.195354598205693 2315 66-67 1.2101146320323437 2315 68-69 1.5517518280854787 2315 70-71 2.0067241456454354 2315 72-73 2.134730913991458 2315 74-75 2.5326844280930523 2315 76-77 2.380996211265451 2315 78-79 2.3997188719737217 2315 80-81 2.7704971998368286 2315 82-83 3.0622906896422357 2315 84-85 3.407699097392843 2315 86-87 3.561985084136385 2315 88-89 3.776145221268507 2315 90-91 4.009596491923542 2315 92-93 4.3452671517514645 2315 94-95 4.392561169626454 2315 96-97 4.814764411838567 2315 98-99 4.924842839992099 2315 100-101 4.361676230236226 2316 1 -0.4365146216027611 2316 2 -0.47482473757073507 2316 3 -0.40635510762910343 2316 4 -0.35725821465635477 2316 5 -0.40809289827917183 2316 6 -0.3658010302572592 2316 7 -0.3599789794775532 2316 8 -0.3902215855071205 2316 9 -0.3960344064968524 2316 10-11 -0.4600203572599426 2316 12-13 -0.5290764451609036 2316 14-15 -0.45924230933647436 2316 16-17 -0.38947682089236935 2316 18-19 -0.5023123245673915 2316 20-21 -0.43224087920860654 2316 22-23 -0.38939914078837745 2316 24-25 -0.37351008175132705 2316 26-27 -0.37993802495202544 2316 28-29 -0.395864801153607 2316 30-31 -0.3230332178632196 2316 32-33 -0.3682467358432078 2316 34-35 -0.4345260814707572 2316 36-37 -0.548674062177291 2316 38-39 -0.6289761326534062 2316 40-41 -0.49602025663156013 2316 42-43 -0.4967704152636969 2316 44-45 -0.3840324726579212 2316 46-47 -0.4986661906631653 2316 48-49 -0.35645403847211554 2316 50-51 -0.20655543405296584 2316 52-53 -0.26820259465377205 2316 54-55 -0.006841125593979314 2316 56-57 0.17883334089908232 2316 58-59 0.40031425869160486 2316 60-61 0.60980012858575 2316 62-63 0.6040094898616601 2316 64-65 0.820549592942033 2316 66-67 0.9606553536324967 2316 68-69 1.4826686622322853 2316 70-71 1.4915492485260948 2316 72-73 1.7684386850700342 2316 74-75 2.237693280614824 2316 76-77 2.248130626041984 2316 78-79 2.3654285092595906 2316 80-81 2.1959840885343134 2316 82-83 2.467508892341055 2316 84-85 2.6013982552069876 2316 86-87 2.7366513201398277 2316 88-89 2.981721113698402 2316 90-91 3.4029056602650094 2316 92-93 3.504978607393152 2316 94-95 4.059061552438209 2316 96-97 4.32555013296021 2316 98-99 4.186670526852087 2316 100-101 4.278426636973714 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 7.0 4 22.0 5 65.0 6 128.0 7 332.0 8 678.0 9 1258.0 10 2217.0 11 3686.0 12 5614.0 13 8425.0 14 11066.0 15 15457.0 16 21265.0 17 30450.0 18 30474.0 19 22465.0 20 21174.0 21 23215.0 22 25489.0 23 28894.0 24 32310.0 25 36546.0 26 40038.0 27 45487.0 28 53835.0 29 63386.0 30 72767.0 31 83146.0 32 96516.0 33 114076.0 34 139479.0 35 177628.0 36 191431.0 37 178075.0 38 58608.0 39 1023.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.17812801477089 19.93672761531661 15.56961337004875 20.315530999863753 2 15.684095068453807 21.712185419537366 40.48194169892545 22.12177781308337 3 19.062131497106964 27.27315496520416 30.939273778174243 22.72543975951463 4 12.383943867812706 18.207035097280638 37.35278375861791 32.05623727628875 5 12.384865171680028 39.16887012357096 33.55279213618189 14.893472568567114 6 29.227876035905698 37.68656078087311 17.47341653978782 15.612146643433377 7 26.566214526132242 35.892903729563706 20.270502274958712 17.270379469345336 8 26.371635674013827 34.96662862337878 19.292101578022546 19.369634124584845 9 27.456219187857762 16.63655587074984 20.424467521581104 35.48275741981129 10-11 24.79039036910724 27.908308807850762 26.1148580739803 21.186442749061698 12-13 26.328515402328907 25.947390319618414 27.48218554889527 20.241908729157412 14-15 22.978152209309645 26.326713031386305 27.657168273628013 23.03796648567604 16-17 20.234668696726956 30.07616391922201 28.083902505784387 21.605264878266645 18-19 20.03717160954169 27.256461499829232 32.665559990542135 20.04080690008694 20-21 21.634805432529312 25.946687700437394 32.8848688209989 19.533638046034387 22-23 21.027605703333542 26.267086691916248 32.76826251415172 19.93704509059849 24-25 20.450313751450686 26.486909380956064 33.15042748463319 19.912349382960063 26-27 20.290670196681415 26.65074575431305 33.18929765191492 19.86928639709061 28-29 19.906851025793458 26.84182453705033 33.24564849612002 20.0056759410362 30-31 20.137035179225933 26.659754523187353 33.19093584598099 20.01227445160573 32-33 20.035277592618954 26.838494733105524 32.900876318862025 20.225351355413498 34-35 20.399363052654476 26.95059014173667 32.7999775137545 19.850069291854357 36-37 20.58837725649194 26.658497900070994 32.73375075302953 20.019374090407535 38-39 20.302507976815605 26.922653911908583 32.70509672996386 20.06974138131195 40-41 20.36691630638318 26.850491442866236 32.650681292480535 20.13191095827005 42-43 20.39483954774402 26.83914203463866 32.31701300133597 20.44900541628135 44-45 20.319519758046038 27.184260772602993 32.0428001894084 20.453419279942565 46-47 20.519480122744554 27.478130971310215 31.318068163245975 20.68432074269925 48-49 20.59043839160083 27.47329588882243 31.19827729996279 20.737988419613952 50-51 20.667818147492596 27.812386015312214 30.698928902881534 20.82086693431366 52-53 20.850010355995753 28.099665614367158 30.3304509654293 20.719873064207782 54-55 20.568260064408793 28.022866283016228 30.37242482432993 21.036448828245046 56-57 20.72109501060955 28.242388954093308 30.108423304228605 20.92809273106854 58-59 20.781550808836872 27.91111928457482 30.301704676327784 21.005625230260524 60-61 20.770889326481473 28.07495174839146 30.40886937576257 20.745289549364497 62-63 20.619734556583143 28.084849514245757 30.341020801804568 20.954395127366528 64-65 20.526561143448564 28.002856910687328 30.29599207689953 21.174589868964578 66-67 20.519944621355236 28.162368671230233 30.092067607891828 21.225619099522707 68-69 20.52985190211041 28.085382882793436 30.127636253327157 21.257128961769002 70-71 20.628929703256425 28.132872007835118 29.90823793495946 21.329960353948994 72-73 20.654926612953975 28.091417476155243 29.745963452805068 21.50769245808571 74-75 20.468488140704686 28.40011168590112 29.42556910626229 21.705831067131903 76-77 20.240656234095603 28.095663739901376 30.043140817714313 21.620539208288704 78-79 20.236959846230267 28.19919314879091 29.940588965946187 21.623258039032635 80-81 20.225815764029857 28.442827401545458 29.745667053107333 21.58568978131735 82-83 20.2792814810289 28.300299382904626 29.680210179018758 21.74020895704772 84-85 20.19853751646638 28.068123773422588 29.593998294095076 22.139340416015955 86-87 20.157458569443286 27.960184140559775 29.736641362461736 22.14571592753521 88-89 20.216461695340655 28.044769672267865 29.456209412286547 22.28255922010493 90-91 20.191288106172596 27.906080154897666 29.739471684832864 22.163160054096874 92-93 20.098556147249518 27.83613933130164 29.53430983203115 22.530994689417692 94-95 19.924007083629782 27.807890298439297 29.515470313854163 22.75263230407675 96-97 19.929560749042526 27.698437646538416 29.477809253147818 22.89419235127124 98-99 19.916046172466736 27.68319573198561 29.24304086249865 23.15771723304901 100-101 20.20589185896539 27.153695807440798 29.46390767461767 23.176504658976143 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 5588.0 1 3553.0 2 2079.0 3 2624.0 4 2042.5 5 1879.5 6 3711.5 7 8072.0 8 12427.5 9 13205.0 10 9765.0 11 6182.5 12 5390.5 13 5384.0 14 5470.0 15 5432.5 16 5347.0 17 5428.5 18 5508.0 19 5724.0 20 6049.0 21 6606.5 22 7397.0 23 8352.5 24 9471.0 25 11003.5 26 13054.0 27 15134.5 28 17252.5 29 19608.0 30 22053.0 31 24573.5 32 27464.5 33 30462.0 34 33396.5 35 35935.5 36 38568.0 37 41332.0 38 43172.0 39 44373.0 40 46083.5 41 48423.5 42 50097.5 43 51931.0 44 53756.0 45 55426.5 46 56243.0 47 56733.0 48 59905.0 49 59663.5 50 55747.0 51 52426.0 52 49326.5 53 47363.0 54 45475.0 55 41778.0 56 37185.0 57 33321.5 58 29747.5 59 26447.5 60 22817.0 61 19205.0 62 16179.0 63 12912.0 64 9897.0 65 7736.5 66 5821.5 67 4574.0 68 3528.0 69 2398.5 70 1733.5 71 1307.5 72 1094.0 73 939.0 74 727.5 75 578.5 76 400.5 77 233.5 78 135.0 79 78.5 80 44.0 81 18.5 82 8.0 83 4.0 84 1.5 85 0.5 86 1.0 87 1.0 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.013930188980120764 3 0.0026271847637947057 4 0.0015274330022062243 5 0.11009737079902464 6 6.109732008824897E-5 7 0.0 8 6.109732008824897E-5 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 3.665839205294938E-4 24-25 4.582299006618673E-4 26-27 0.0062013779889572695 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.010814225655620067 36-37 6.720705209707386E-4 38-39 0.0070261918101486315 40-41 0.010661482355399446 42-43 0.005712599428251279 44-45 0.00354364456511844 46-47 3.0548660044124486E-5 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 6.109732008824897E-5 68-69 7.026191810148632E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 3.0548660044124486E-5 82-83 0.002016211562912216 84-85 0.003971325805736183 86-87 0.0029632200242800753 88-89 0.0 90-91 0.0024133441434858343 92-93 6.109732008824897E-5 94-95 9.164598013237345E-5 96-97 2.1384062030887138E-4 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1636733.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.974367129343925 #Duplication Level Percentage of deduplicated Percentage of total 1 73.66111944494791 38.28490065190177 2 14.233389920824582 14.795428664800806 3 5.186877926063427 8.087540927529316 4 2.443784639114217 5.080566400734942 5 1.3020520345398772 3.3836665232342384 6 0.7512964895318333 2.3428895741948876 7 0.46420968013361485 1.6888903038181868 8 0.3320386026434826 1.3805996987925369 9 0.25655943542848875 1.200106285871173 >10 1.2437719069123214 11.542694518809563 >50 0.08210821567558166 2.9628741046005223 >100 0.03867732380274162 3.5267498809251516 >500 0.002703735679518582 1.0008431389189343 >1k 9.404298015716807E-4 1.1485776307024629 >5k 1.1755372519646008E-4 0.3203357900831434 >10k+ 3.5266117558938027E-4 3.2533359050824378 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 22264 1.360270734444775 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 18173 1.1103215979637486 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12811 0.7827177676505574 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5243 0.32033324922268935 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3990 0.24377830715211338 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3959 0.24188429022937766 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2290 0.13991286300209016 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2277 0.1391185978409429 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1966 0.12011733129349747 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1928 0.117795633130144 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 6.109732008824897E-5 0.0 12-13 0.0 0.0 0.0 6.109732008824897E-5 0.0 14-15 0.0 0.0 0.0 6.109732008824897E-5 0.0 16-17 0.0 0.0 0.0 1.2219464017649794E-4 0.0 18-19 0.0 0.0 0.0 1.2219464017649794E-4 0.0 20-21 0.0 0.0 0.0 2.443892803529959E-4 0.0 22-23 0.0 0.0 0.0 7.026191810148631E-4 0.0 24-25 0.0 0.0 0.0 0.0015885303222944731 0.0 26-27 0.0 0.0 0.0 0.0037574851854273116 0.0 28-29 0.0 0.0 0.0 0.008767465432663726 0.0 30-31 0.0 0.0 0.0 0.02364466287415235 0.0 32-33 0.0 0.0 0.0 0.051077359593776135 0.0 34-35 0.0 0.0 0.0 0.09277628055400605 0.0 36-37 0.0 0.0 0.0 0.15634804210582912 0.0 38-39 0.0 0.0 0.0 0.24949090658036466 0.0 40-41 0.0 0.0 0.0 0.3547310404323735 0.0 42-43 0.0 0.0 0.0 0.45972678500402936 6.109732008824897E-5 44-45 0.0 0.0 0.0 0.5782555859752323 6.109732008824897E-5 46-47 0.0 0.0 0.0 0.7196958819795287 6.109732008824897E-5 48-49 0.0 0.0 0.0 0.8738444205621809 6.109732008824897E-5 50-51 0.0 0.0 0.0 1.038318406239747 6.109732008824897E-5 52-53 0.0 0.0 0.0 1.195888394747341 6.109732008824897E-5 54-55 0.0 0.0 0.0 1.3600568938244662 6.109732008824897E-5 56-57 0.0 0.0 0.0 1.5464037200936254 6.109732008824897E-5 58-59 0.0 0.0 0.0 1.7477804871044942 6.109732008824897E-5 60-61 0.0 0.0 0.0 1.9668449282809108 6.109732008824897E-5 62-63 0.0 0.0 0.0 2.1783333017663846 6.109732008824897E-5 64-65 0.0 0.0 0.0 2.387225039148108 6.109732008824897E-5 66-67 0.0 0.0 0.0 2.620311315284778 6.109732008824897E-5 68-69 0.0 0.0 0.0 2.87771432481657 6.109732008824897E-5 70-71 0.0 0.0 0.0 3.157142918240177 6.109732008824897E-5 72-73 0.0 0.0 0.0 3.4277124002509876 6.109732008824897E-5 74-75 0.0 0.0 0.0 3.693058061394253 6.109732008824897E-5 76-77 0.0 0.0 0.0 3.9785352895065964 6.109732008824897E-5 78-79 0.0 0.0 0.0 4.291536860318696 6.109732008824897E-5 80-81 6.109732008824897E-5 0.0 0.0 4.638966771000524 6.109732008824897E-5 82-83 6.109732008824897E-5 0.0 0.0 4.97735427830929 6.109732008824897E-5 84-85 6.109732008824897E-5 0.0 0.0 5.3095404076291 6.109732008824897E-5 86-87 6.109732008824897E-5 0.0 0.0 5.647164198436764 6.109732008824897E-5 88-89 6.109732008824897E-5 0.0 0.0 6.007180157056771 6.109732008824897E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 9055 0.0 47.73259 1 GTATCAA 14750 0.0 37.458782 1 TATCAAC 18595 0.0 29.483067 2 ATCAACG 18790 0.0 29.09851 3 TCAACGC 19090 0.0 28.640352 4 CAACGCA 19465 0.0 28.088585 5 AACGCAG 19830 0.0 27.590836 6 GTCGACG 45 8.950153E-4 26.389364 24-25 GTACATG 12980 0.0 24.287252 1 ACGCAGA 22400 0.0 24.276863 7 CGCAGAG 22530 0.0 24.073544 8 GAGTACT 13270 0.0 23.836275 12-13 TACATGG 13120 0.0 23.30326 2 GCAGAGT 23000 0.0 23.189266 9 GTACTTT 14075 0.0 22.489864 14-15 GTGGTAT 4470 0.0 22.125616 1 ACATGGG 13560 0.0 21.984041 3 AGTACTT 13395 0.0 21.841028 12-13 TGGTATC 4555 0.0 21.608343 2 CAGAGTA 22790 0.0 20.63127 10-11 >>END_MODULE