FastQCFastQC Report
Thu 26 May 2016
SRR937934_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937934_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1636248
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT238911.46010873657294No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT177671.0858378436520626No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT125190.765104067354093No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA52140.3186558516801854No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39540.24165040996230402No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37180.22722716849768493No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24880.15205518967784834No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24820.1516884970982394No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20560.1256533239460033No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19010.11618043230610518No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA91150.046.8411061
GTATCAA151750.038.6038321
TATCAAC191850.030.371462
ATCAACG192650.030.146663
TCAACGC195450.029.7390964
CAACGCA197750.029.417245
AACGCAG201450.028.8008756
TATAACG1056.881288E-527.1580732
GTACATG126300.026.6284921
TACATGG126650.025.9679662
ACGCAGA223600.025.9399057
CGCAGAG225700.025.592538
ACATGGG128800.024.8334033
GAGTACT134300.024.72363712-13
GCAGAGT231250.024.6701899
GTACTTT141700.023.35011714-15
AGTACTT135050.023.33767312-13
CATGGGG39450.022.8898854
CATGGGA83550.022.8105074
TAGACTG6200.021.4636385
Produced by FastQC (version 0.11.5)