FastQCFastQC Report
Thu 26 May 2016
SRR937934_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937934_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1636248
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT230731.4101163148862519No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT182171.1133397871227346No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT133350.8149742581809114No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA51650.3156611956133789No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41560.25399572680913896No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40370.2467229906468946No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23770.1452713769550826No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23320.1425211826080154No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21140.12919801888222324No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20780.12699786340456948No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA96350.044.0263441
GTATCAA156100.036.151511
TATCAAC196000.028.7436242
ATCAACG200350.028.1406733
TCAACGC203500.027.7284184
CAACGCA206700.027.2991475
AACGCAG209550.026.9731926
GTACATG125800.024.6572951
CGATCGT1550.024.51796524-25
GAGTACT135650.024.44042212-13
ACGCAGA234050.024.0887997
TACATGG127500.023.9559652
CGCAGAG235250.023.9255438
GCAGAGT240350.023.1411979
ACATGGG129000.022.7179183
GTACTTT143600.022.7069714-15
AGTACTT138350.022.4528612-13
CATGGGA83900.020.833344
AGAGTAC217550.020.5776310-11
ACTTTTT160500.020.39000116-17
Produced by FastQC (version 0.11.5)