##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937934_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1636248 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.1762929580357 33.0 31.0 34.0 31.0 34.0 2 32.423536040991344 34.0 31.0 34.0 31.0 34.0 3 32.373748355994934 34.0 31.0 34.0 30.0 34.0 4 36.01442018569312 37.0 35.0 37.0 35.0 37.0 5 35.979039240995256 37.0 35.0 37.0 35.0 37.0 6 36.05940297558805 37.0 35.0 37.0 35.0 37.0 7 36.00673247576162 37.0 35.0 37.0 35.0 37.0 8 36.01340078032181 37.0 35.0 37.0 35.0 37.0 9 37.62881115821074 39.0 37.0 39.0 35.0 39.0 10-11 37.58521141049523 39.0 37.0 39.0 35.0 39.0 12-13 37.50344446563113 39.0 37.0 39.0 35.0 39.0 14-15 38.5676605869037 40.0 38.0 41.0 34.0 41.0 16-17 38.50119083415228 40.0 38.0 41.0 34.0 41.0 18-19 38.56377700690849 40.0 38.0 41.0 34.0 41.0 20-21 38.6134146535244 40.0 38.0 41.0 34.0 41.0 22-23 38.600461543726865 40.0 38.0 41.0 34.0 41.0 24-25 38.52265854564834 40.0 38.0 41.0 34.0 41.0 26-27 38.30022343801184 40.0 38.0 41.0 34.0 41.0 28-29 38.17825812468526 40.0 37.5 41.0 33.5 41.0 30-31 37.851499283727165 40.0 37.0 41.0 33.0 41.0 32-33 37.53239026113401 39.5 36.0 41.0 33.0 41.0 34-35 37.255347905696446 39.0 35.0 41.0 33.0 41.0 36-37 37.10692877852257 39.0 35.0 41.0 32.0 41.0 38-39 37.00758381370061 39.0 35.0 41.0 32.0 41.0 40-41 36.820089619666454 39.0 35.0 41.0 31.5 41.0 42-43 36.529441441639655 39.0 35.0 40.5 31.0 41.0 44-45 36.11660304550411 39.0 35.0 40.0 30.0 41.0 46-47 35.53613144217747 38.5 35.0 40.0 28.0 41.0 48-49 34.968632505585944 38.0 35.0 40.0 26.0 41.0 50-51 34.583162821283814 38.0 34.0 40.0 24.0 41.0 52-53 34.03885505131252 38.0 33.0 40.0 23.0 41.0 54-55 33.670434127345 37.5 33.0 40.0 22.0 41.0 56-57 33.36226507228733 37.0 33.0 40.0 20.0 41.0 58-59 32.941595956114234 36.5 32.5 39.5 18.5 41.0 60-61 32.46818850198747 36.0 31.5 39.0 16.5 40.0 62-63 31.98141479775682 35.5 31.0 39.0 12.5 40.0 64-65 31.49694270061751 35.0 30.5 38.5 8.0 40.0 66-67 30.915774381389618 35.0 29.5 38.0 7.5 40.0 68-69 30.324595354738403 34.5 29.0 37.5 4.0 39.5 70-71 29.675284247864628 34.0 28.0 37.0 2.0 39.0 72-73 29.147070004058065 34.0 27.5 36.0 2.0 39.0 74-75 28.533347939921086 33.0 26.0 36.0 2.0 38.5 76-77 27.47646414235495 31.5 26.0 34.5 2.0 36.5 78-79 28.20831041504711 33.0 27.0 35.0 2.0 37.0 80-81 27.980763307273715 33.0 26.5 35.0 2.0 37.0 82-83 27.436494345600423 33.0 26.0 35.0 2.0 36.0 84-85 26.81705737761024 32.5 25.0 35.0 2.0 36.0 86-87 26.13188312529641 32.0 23.5 34.5 2.0 35.0 88-89 25.36208417061472 31.0 20.5 34.0 2.0 35.0 90-91 24.662672162166125 31.0 18.5 34.0 2.0 35.0 92-93 23.835966797209224 31.0 10.0 34.0 2.0 35.0 94-95 22.85796682410001 30.0 2.0 34.0 2.0 35.0 96-97 21.77884373273489 29.0 2.0 34.0 2.0 35.0 98-99 20.5140632715823 29.0 2.0 34.0 2.0 35.0 100-101 17.329965567566774 22.5 2.0 31.0 2.0 34.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.13718115759550642 1101 2 0.03771576401157972 1101 3 0.2648158695167311 1101 4 0.07859027125434181 1101 5 0.0875464099896135 1101 6 0.14282237647464768 1101 7 0.16563639519234385 1101 8 0.195227308381277 1101 9 0.18510603244952506 1101 10-11 0.2970851878546412 1101 12-13 0.16874561950926648 1101 14-15 0.34815872284517724 1101 16-17 0.30753289774484927 1101 18-19 0.3560957049860036 1101 20-21 0.2751836665040557 1101 22-23 0.17972484087018614 1101 24-25 0.28463312795928886 1101 26-27 0.3879247610840437 1101 28-29 0.1829674694601806 1101 30-31 0.3548952796318119 1101 32-33 0.38586915371663366 1101 34-35 0.2482462436865731 1101 36-37 0.27636233485991823 1101 38-39 0.48321445801921925 1101 40-41 0.27607383006487396 1101 42-43 0.32240202664997497 1101 44-45 0.26255238511314616 1101 46-47 0.29117102591004596 1101 48-49 0.2716369523376514 1101 50-51 0.3880301032229525 1101 52-53 0.36676595800445 1101 54-55 0.35918080520735884 1101 56-57 0.5327510271246467 1101 58-59 0.6309666054211647 1101 60-61 0.7650830137695834 1101 62-63 0.906858986030965 1101 64-65 0.8248052221681235 1101 66-67 0.9134473160237953 1101 68-69 1.007209152424796 1101 70-71 1.1657328913820884 1101 72-73 1.1670866913329974 1101 74-75 1.0901033862031326 1101 76-77 1.104654547876681 1101 78-79 1.328859574286124 1101 80-81 1.659944664109993 1101 82-83 1.7978134353728343 1101 84-85 1.9999007154159685 1101 86-87 1.8351093444654687 1101 88-89 2.2483423090292014 1101 90-91 2.511925308097293 1101 92-93 2.7103555605718093 1101 94-95 2.6223880840072056 1101 96-97 3.026769985405238 1101 98-99 3.141584425636765 1101 100-101 2.7578296605598744 1102 1 0.19977514852161704 1102 2 0.22645906170503594 1102 3 0.34155189721884227 1102 4 0.06784165250737573 1102 5 0.04956251818738622 1102 6 0.11329868781519536 1102 7 0.10424394709981044 1102 8 0.16358652935003448 1102 9 0.16960925726176868 1102 10-11 0.3397274943092512 1102 12-13 0.264602087036792 1102 14-15 0.32961639236877716 1102 16-17 0.3222333278708973 1102 18-19 0.37136087384106986 1102 20-21 0.3130828021727936 1102 22-23 0.2983433158943214 1102 24-25 0.3861240653983842 1102 26-27 0.42177667806836183 1102 28-29 0.35164713206732046 1102 30-31 0.5079986231932807 1102 32-33 0.6303702029703757 1102 34-35 0.6337271101027468 1102 36-37 0.7079933692020077 1102 38-39 0.6297401364082162 1102 40-41 0.6670887734323685 1102 42-43 0.6503327985653229 1102 44-45 0.6580591769348558 1102 46-47 0.8006723244465306 1102 48-49 0.8957638961765113 1102 50-51 0.9180063533759295 1102 52-53 0.842766601607174 1102 54-55 0.9198451210621954 1102 56-57 1.14054292588866 1102 58-59 1.2835707036295005 1102 60-61 1.246573769912139 1102 62-63 1.1612758230408424 1102 64-65 1.3862912646005867 1102 66-67 1.4187923913298413 1102 68-69 1.4330851546547407 1102 70-71 1.5518140759736916 1102 72-73 1.615177638743404 1102 74-75 1.7439849451720981 1102 76-77 1.9009746813109345 1102 78-79 1.9600690941675545 1102 80-81 2.0064569718790004 1102 82-83 1.867667446332213 1102 84-85 1.8806823754577557 1102 86-87 2.098204167089463 1102 88-89 2.2116374643323518 1102 90-91 2.2043092669792337 1102 92-93 2.5154710691312694 1102 94-95 2.7618896544885025 1102 96-97 2.7885866674065696 1102 98-99 3.191355538752209 1102 100-101 3.5245941518191195 1103 1 0.34983615045491234 1103 2 0.3559145490635558 1103 3 0.3975798267193795 1103 4 0.19102801810603864 1103 5 0.10403255216077412 1103 6 0.15822614243914046 1103 7 0.23831305759777166 1103 8 0.25428232678607543 1103 9 0.33049294065234847 1103 10-11 0.35575317143071317 1103 12-13 0.1934569399752064 1103 14-15 0.5066759114858428 1103 16-17 0.39909388683630453 1103 18-19 0.4687668839392387 1103 20-21 0.4579164076685984 1103 22-23 0.43905882164727217 1103 24-25 0.6117092147123415 1103 26-27 0.5544155278534433 1103 28-29 0.5353530464393685 1103 30-31 0.7010913273620645 1103 32-33 0.7273538919919034 1103 34-35 0.7849330713993723 1103 36-37 0.8695978138967178 1103 38-39 0.9039466420297799 1103 40-41 0.9898134013205748 1103 42-43 0.9871310417096453 1103 44-45 1.0884156159235303 1103 46-47 1.0552906400423865 1103 48-49 1.3449258234622121 1103 50-51 1.4479536101659107 1103 52-53 1.5331433120075317 1103 54-55 1.501809195615941 1103 56-57 1.6284964518004017 1103 58-59 1.6610694366662742 1103 60-61 1.760538520179601 1103 62-63 1.5955720484289024 1103 64-65 1.6646308858255026 1103 66-67 1.4839950115975036 1103 68-69 1.6198693204298387 1103 70-71 1.7071156132224132 1103 72-73 1.6658542909573022 1103 74-75 1.8282539543130056 1103 76-77 1.6289435984796903 1103 78-79 2.022082721060965 1103 80-81 2.219199806498068 1103 82-83 2.308262825201144 1103 84-85 2.0974081670751517 1103 86-87 2.2959048634499943 1103 88-89 2.516707194234563 1103 90-91 2.50985387346984 1103 92-93 2.8628123233310205 1103 94-95 2.6498788430689437 1103 96-97 3.146412746202298 1103 98-99 3.2752829370666774 1103 100-101 3.0395687187053895 1104 1 0.11821238033410708 1104 2 0.16607554477317166 1104 3 0.24404871665315397 1104 4 0.16629347573022812 1104 5 0.14853890864227282 1104 6 0.0596790734223589 1104 7 0.09155223385518951 1104 8 0.09218509070932868 1104 9 0.1578931184785617 1104 10-11 0.16189257019060932 1104 12-13 0.10298895235566619 1104 14-15 0.2147267223235758 1104 16-17 0.2045455639542908 1104 18-19 0.1769443996162181 1104 20-21 0.11847780531818586 1104 22-23 0.12041372849358112 1104 24-25 0.21405596411067762 1104 26-27 0.2511976643753471 1104 28-29 0.22345114088647478 1104 30-31 0.18118841402964136 1104 32-33 0.29231978422101434 1104 34-35 0.35285492161228404 1104 36-37 0.2632250731611876 1104 38-39 0.19114183632761694 1104 40-41 0.24571396512048693 1104 42-43 0.24330259273800436 1104 44-45 0.5060018081721296 1104 46-47 0.5318290052423436 1104 48-49 0.5010049458737313 1104 50-51 0.7423255716300616 1104 52-53 0.6265460180713447 1104 54-55 0.7348933671786853 1104 56-57 0.968367901318409 1104 58-59 0.9722440710898539 1104 60-61 1.059843913997291 1104 62-63 0.8407290672178469 1104 64-65 0.8001464719855278 1104 66-67 1.0286192841432715 1104 68-69 1.2018810417260681 1104 70-71 1.160904966793364 1104 72-73 1.113834264268725 1104 74-75 1.3822785540941638 1104 76-77 1.258033125335995 1104 78-79 1.4644708362935184 1104 80-81 1.6141102269091192 1104 82-83 1.608869690113277 1104 84-85 1.6417219029050827 1104 86-87 1.6318982660095784 1104 88-89 1.9987180366532904 1104 90-91 1.9857426017194548 1104 92-93 2.212754976574299 1104 94-95 2.5573257185861955 1104 96-97 2.897019537874641 1104 98-99 3.1673392028040794 1104 100-101 3.2109586195240674 1105 1 0.1464052152653892 1105 2 0.15180079258412604 1105 3 0.20142780516698622 1105 4 0.18779332149969008 1105 5 0.11901545019553339 1105 6 0.1998052198663416 1105 7 0.13777826151809336 1105 8 0.15336694971833253 1105 9 0.19754250951260133 1105 10-11 0.22173474396040405 1105 12-13 0.15037270584488738 1105 14-15 0.1756009176878166 1105 16-17 0.23292507039482047 1105 18-19 0.2188146379069309 1105 20-21 0.26075393633911403 1105 22-23 0.3021031020276226 1105 24-25 0.28319673508938337 1105 26-27 0.2548997409962013 1105 28-29 0.332284074235325 1105 30-31 0.28110424873944595 1105 32-33 0.27075705008872575 1105 34-35 0.3624277265818776 1105 36-37 0.47304475573262295 1105 38-39 0.487712662579213 1105 40-41 0.5318915379341576 1105 42-43 0.5627312546802514 1105 44-45 0.6349042848137216 1105 46-47 0.6798390325833807 1105 48-49 0.7521433702284597 1105 50-51 0.9086964636335182 1105 52-53 0.8349464534690512 1105 54-55 0.8270927816463427 1105 56-57 0.8797812148754431 1105 58-59 0.9212961604152454 1105 60-61 0.918885357609625 1105 62-63 0.7767128822542837 1105 64-65 1.0360849421563216 1105 66-67 1.2274461896334792 1105 68-69 1.0393488771376163 1105 70-71 1.354470091770846 1105 72-73 1.4105228929290732 1105 74-75 1.265928377191191 1105 76-77 1.285351503925387 1105 78-79 1.5811291118039925 1105 80-81 1.7056454170258952 1105 82-83 1.669968543732729 1105 84-85 1.922808780550426 1105 86-87 2.0079583517624826 1105 88-89 2.10797143398414 1105 90-91 2.0287968448353517 1105 92-93 2.5063544636082398 1105 94-95 2.69251793768273 1105 96-97 2.8508074652663105 1105 98-99 2.704485926972822 1105 100-101 2.748113318009704 1106 1 -0.011826812450131285 1106 2 0.02356130262051437 1106 3 0.14559323135071622 1106 4 0.0971567365002528 1106 5 0.004024353174699513 1106 6 -0.0505115122811759 1106 7 -0.052768009521592774 1106 8 -0.1097517070629408 1106 9 -0.18214131774462317 1106 10-11 -0.09699507656919337 1106 12-13 -0.10193712557023105 1106 14-15 -0.06184581132492184 1106 16-17 0.05019551303173131 1106 18-19 -0.11951510620586703 1106 20-21 -0.028691046204997406 1106 22-23 -0.00539446948734934 1106 24-25 -0.01602340545138503 1106 26-27 -0.03601834068884813 1106 28-29 -0.11502844337800866 1106 30-31 -0.05299477715747258 1106 32-33 0.03327654569564231 1106 34-35 -0.18021720938035912 1106 36-37 0.05874960746046298 1106 38-39 0.27657326067787835 1106 40-41 0.22646552308073353 1106 42-43 0.2005956725633311 1106 44-45 0.3325546441872902 1106 46-47 -0.052491429959900415 1106 48-49 0.13956562968178332 1106 50-51 0.32558492782827386 1106 52-53 0.2299303521119498 1106 54-55 0.30168877004591366 1106 56-57 0.40771908365597653 1106 58-59 0.4399626872893734 1106 60-61 0.45598339100292407 1106 62-63 0.5006251830719002 1106 64-65 0.5648288463158693 1106 66-67 0.5515973105363265 1106 68-69 0.18524462309327916 1106 70-71 0.7674819515031004 1106 72-73 0.841361644689524 1106 74-75 0.9830410958384057 1106 76-77 0.9872139346902564 1106 78-79 1.0168832368861906 1106 80-81 1.2301411875390755 1106 82-83 1.1375213098484238 1106 84-85 1.368369728048357 1106 86-87 1.5067593257600116 1106 88-89 1.7968755530676894 1106 90-91 1.506436839389071 1106 92-93 1.775837668624444 1106 94-95 2.0021947248841094 1106 96-97 1.9723499305946497 1106 98-99 2.09492633330472 1106 100-101 2.288746331966326 1107 1 0.05324366647637646 1107 2 0.0733836556879055 1107 3 0.151349252647627 1107 4 0.052315716384754296 1107 5 -0.012978017344899229 1107 6 -0.0398323097890696 1107 7 -0.031394231084462376 1107 8 0.0550373374134594 1107 9 0.05808459180399694 1107 10-11 0.055573743862531444 1107 12-13 0.08593866762474534 1107 14-15 0.1986730560793859 1107 16-17 0.19650633550812557 1107 18-19 0.0689360217544035 1107 20-21 0.14279915271745836 1107 22-23 0.15544911197908817 1107 24-25 0.14451905686110678 1107 26-27 0.1774423873243549 1107 28-29 0.21230946611981238 1107 30-31 0.10132895634572492 1107 32-33 0.07907372774172217 1107 34-35 0.09352115650849413 1107 36-37 0.2595135343884394 1107 38-39 0.30787411213322 1107 40-41 0.3058149319213328 1107 42-43 0.23665594451971117 1107 44-45 0.14979720819849973 1107 46-47 0.36787725886169653 1107 48-49 0.3350830315949693 1107 50-51 0.3562268931437629 1107 52-53 0.4019200211515894 1107 54-55 0.3471445611754973 1107 56-57 0.45810457298797047 1107 58-59 0.5145272760110728 1107 60-61 0.6768394182821069 1107 62-63 0.7481112057624522 1107 64-65 0.6719896670042544 1107 66-67 0.6871131283154561 1107 68-69 0.9086367687961818 1107 70-71 0.9600747211008276 1107 72-73 0.6541738135127666 1107 74-75 0.8487128772974195 1107 76-77 0.6900840246684012 1107 78-79 1.0366491526710853 1107 80-81 1.139021729459973 1107 82-83 1.484129174171688 1107 84-85 1.5558488193168714 1107 86-87 1.5591537243421776 1107 88-89 1.8925629318973094 1107 90-91 1.9090914670824795 1107 92-93 2.082768656191668 1107 94-95 1.808397311567827 1107 96-97 1.9271381094788147 1107 98-99 2.243740020757741 1107 100-101 2.1940677524332735 1108 1 0.017973870499410793 1108 2 0.017693610207025756 1108 3 0.08925849085566995 1108 4 0.008658299719371598 1108 5 -0.016149373105896814 1108 6 -0.06102488825256813 1108 7 -0.027936696999439903 1108 8 -0.014520980152823881 1108 9 -0.03500860811190876 1108 10-11 -0.02515626403381077 1108 12-13 -0.005250469007620495 1108 14-15 0.0711977577873455 1108 16-17 0.15108778448698246 1108 18-19 0.09802462230715037 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66-67 -0.9996002007391454 2316 68-69 -1.0147553769067237 2316 70-71 -1.2976999552696782 2316 72-73 -1.2903597543434664 2316 74-75 -1.4743131038842385 2316 76-77 -1.3347387208237969 2316 78-79 -1.1332351399456826 2316 80-81 -1.5598327008431312 2316 82-83 -2.1400965672395706 2316 84-85 -2.138865938672449 2316 86-87 -2.0961851027203906 2316 88-89 -2.471675252144639 2316 90-91 -2.719837195168214 2316 92-93 -3.0986485321976325 2316 94-95 -3.080329543928123 2316 96-97 -3.5366421796988234 2316 98-99 -4.040106962131368 2316 100-101 -3.720921681926553 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 6.0 4 24.0 5 62.0 6 115.0 7 276.0 8 624.0 9 1122.0 10 1885.0 11 3142.0 12 4866.0 13 7313.0 14 9819.0 15 13002.0 16 18713.0 17 27053.0 18 26155.0 19 16674.0 20 14328.0 21 14804.0 22 15483.0 23 17185.0 24 19345.0 25 22861.0 26 26424.0 27 31516.0 28 37865.0 29 46061.0 30 56047.0 31 68013.0 32 85678.0 33 109044.0 34 145746.0 35 207619.0 36 245951.0 37 245639.0 38 93301.0 39 2484.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.170871408246185 19.889222171700133 15.641638675799758 20.298267744253927 2 15.688317879823238 21.72423277448062 40.41880336656297 22.168645979133178 3 19.245160320310582 27.267409950848386 30.910936692087947 22.576493036753085 4 12.427402464297872 18.19676369154535 37.362390885972594 32.013442958184186 5 12.313261347329615 39.26572076047338 33.46970256269847 14.951315329498534 6 29.22739001348208 37.707777436889074 17.471272657045457 15.59355989258339 7 26.54554810762183 35.87200717739609 20.303340324938517 17.27910439004356 8 26.416977387888256 34.920094582297175 19.27025687442055 19.39267115539402 9 27.455617974781326 16.59247253472579 20.422698759601236 35.52921073089165 10-11 24.81900665424801 27.963731659259476 26.065364174623895 21.15189751186862 12-13 26.34435611227638 25.983866748805806 27.487520229207306 20.184256909710506 14-15 22.98725498824139 26.32058221003173 27.673372251639115 23.01879055008776 16-17 20.202010942106575 30.062955004375862 28.077314685793354 21.65771936772421 18-19 20.0107807618405 27.174242535361387 32.79872610997844 20.01625059281967 20-21 21.633792676904722 25.845348626858524 33.0509189316045 19.469939764632258 22-23 21.014968391099636 26.21363020764578 32.83090338383913 19.940498017415454 24-25 20.40698521873347 26.458134005285945 33.22910978400607 19.90577099197452 26-27 20.26548210054284 26.668050181869084 33.23951307192638 19.826954645661687 28-29 19.872726689350753 26.853324003013 33.30227640462829 19.97167290300796 30-31 20.084351593723806 26.644885107470056 33.26491454679964 20.005848752006496 32-33 19.983699971916526 26.82072749952863 33.01266017741789 20.182912351136952 34-35 20.354789898964583 26.908170821642035 32.88521070005358 19.851828579339802 36-37 20.523289234392223 26.614211632744677 32.816677632574155 20.045821500288945 38-39 20.221438506741844 26.867436112399023 32.80891499960726 20.102210381251872 40-41 20.264636151271482 26.81818918134991 32.771096636635406 20.1460780307432 42-43 20.351993545104044 26.79017156514417 32.4681572219497 20.38967766780208 44-45 20.28132031747322 27.084697725973218 32.19903472053928 20.43494723601428 46-47 20.494452588247196 27.42964166962319 31.439265108129227 20.636640634000393 48-49 20.608595794509615 27.428736091605032 31.303346326521382 20.65932178736397 50-51 20.671408001721012 27.80339532882546 30.72266551280735 20.802531156646182 52-53 20.800697693748134 28.064755464941744 30.47527025243117 20.659276588878946 54-55 20.502026587656637 28.016963198732707 30.47316177009842 21.00784844351223 56-57 20.65557910536789 28.188086402550223 30.29183228948179 20.864502202600093 58-59 20.71394434095565 27.840278490791125 30.46784472769409 20.977932440559133 60-61 20.747405038844967 28.033005999090605 30.536202336076197 20.683386625988238 62-63 20.566228346803175 28.07367831771223 30.50692804513741 20.853165290347185 64-65 20.425204492228563 27.971340530286366 30.458707970918837 21.14474700656624 66-67 20.40821565012043 28.142641404730227 30.275668100642754 21.173474844506593 68-69 20.406057896604082 28.100995908618792 30.272217874529144 21.220728320247982 70-71 20.48852007764104 28.147933565083044 30.077867169279966 21.28567918799595 72-73 20.534539996381966 28.10414435953474 29.897118285247714 21.46419735883558 74-75 20.314310544611818 28.421486229471327 29.607461705071604 21.65674152084525 76-77 20.17961213703546 28.177482875456533 30.031480557959426 21.611424429548578 78-79 20.21695366472564 28.213724325407885 29.967951068542177 21.601370941324298 80-81 20.113858254723468 28.55296731235237 29.82814309593001 21.505031336994154 82-83 20.143732322232243 28.35434497224117 29.75906287357081 21.742859831955784 84-85 20.111350795343178 28.113218559932722 29.70589125099584 22.06953939372826 86-87 20.093091677150582 28.027274785765993 29.81060500728348 22.06902852979994 88-89 20.0924676737474 27.96578757186795 29.714392229781865 22.227352524602786 90-91 20.023432550893528 27.988513527347603 29.86770143116413 22.12035249059474 92-93 20.04100259253238 27.897714146719487 29.71523702542051 22.346046235327623 94-95 19.82553356891323 27.83805124657295 29.695718125514137 22.640697058999688 96-97 19.870214876396723 27.700683822635146 29.69140280508458 22.737698495883556 98-99 19.822997005030118 27.737619736915686 29.557856150383483 22.88152710767071 100-101 20.028388819877673 27.35041246296695 29.70870137192257 22.9124973452328 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 5647.0 1 3616.5 2 2133.5 3 2704.0 4 2114.0 5 1958.0 6 3966.0 7 8552.5 8 13098.0 9 13753.0 10 10019.5 11 6389.5 12 5509.0 13 5467.0 14 5561.0 15 5506.5 16 5434.0 17 5419.5 18 5506.5 19 5695.5 20 5918.0 21 6427.5 22 7209.0 23 8195.5 24 9326.0 25 10795.0 26 12804.5 27 15077.0 28 17165.0 29 19265.5 30 21886.0 31 24458.0 32 27288.5 33 30529.5 34 33534.5 35 35736.0 36 38448.5 37 41410.5 38 43039.5 39 44252.5 40 45981.0 41 48223.5 42 50229.5 43 52311.5 44 54171.5 45 55963.0 46 56560.0 47 57222.5 48 60893.0 49 60310.5 50 55943.5 51 52598.5 52 49409.0 53 47517.0 54 45283.5 55 41590.0 56 37279.0 57 33077.0 58 29326.5 59 26010.0 60 22254.0 61 18862.0 62 16141.5 63 12583.0 64 9408.0 65 7290.5 66 5428.5 67 4353.5 68 3299.0 69 2141.5 70 1539.5 71 1206.5 72 991.5 73 825.5 74 700.5 75 553.5 76 359.0 77 195.5 78 100.0 79 57.0 80 25.5 81 11.5 82 8.0 83 6.0 84 4.0 85 3.0 86 2.0 87 1.0 88 1.5 89 1.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.009656237929702587 3 0.0052559269743950796 4 6.111542993482651E-5 5 2.4446171973930604E-4 6 1.2223085986965302E-4 7 0.0 8 6.111542993482651E-5 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0013139817435987699 26-27 0.019465264434242242 28-29 3.361348646415458E-4 30-31 9.167314490223976E-5 32-33 0.0033307909314480447 34-35 0.014514914609521295 36-37 0.0071199475874072875 38-39 0.018854110134893975 40-41 0.02087091932274325 42-43 0.017142878096718835 44-45 0.00864783333577795 46-47 0.0021084823327515143 48-49 3.6669257960895903E-4 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 3.0557714967413254E-4 68-69 2.1390400477189276E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 1.8334628980447952E-4 82-83 7.333851592179181E-4 84-85 0.00412529152060079 86-87 7.028274442505048E-4 88-89 3.0557714967413255E-5 90-91 0.0038197143709266567 92-93 6.111542993482651E-4 94-95 1.2223085986965302E-4 96-97 3.0557714967413254E-4 98-99 2.750194347067193E-4 100-101 0.0024751749123604738 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1636248.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.845901464159894 #Duplication Level Percentage of deduplicated Percentage of total 1 73.57769066657248 38.14701700259554 2 14.328400380613962 14.85737668544685 3 5.150851226525202 8.011515754409185 4 2.382771660115034 4.9414777880766705 5 1.3027602320868426 3.377138931210026 6 0.7646047815960608 2.378497449939292 7 0.5273527673640613 1.913875572952641 8 0.3371028629010257 1.3981921450602224 9 0.25311443576841813 1.181065148640525 >10 1.2488282348523556 11.474095458204385 >50 0.08456727142036337 3.0290228615792643 >100 0.03783084389341167 3.4592198780023113 >500 0.0027104752768382905 0.9924874531783687 >1k 9.427740093350575E-4 1.1859259705855785 >5k 1.1784675116688219E-4 0.31557483967411193 >10k+ 3.535402535006466E-4 3.337517060444988 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 23073 1.4101163148862519 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 18217 1.1133397871227346 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13335 0.8149742581809114 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5165 0.3156611956133789 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4156 0.25399572680913896 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4037 0.2467229906468946 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2377 0.1452713769550826 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2332 0.1425211826080154 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2114 0.12919801888222324 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2078 0.12699786340456948 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 3.0557714967413255E-5 0.0 18-19 0.0 0.0 0.0 1.2223085986965302E-4 0.0 20-21 0.0 0.0 0.0 1.8334628980447954E-4 0.0 22-23 0.0 0.0 0.0 4.5836572451119883E-4 0.0 24-25 0.0 0.0 0.0 0.0012834240286313566 0.0 26-27 0.0 0.0 0.0 0.0035446949362199372 0.0 28-29 0.0 0.0 0.0 0.008953410485452083 0.0 30-31 0.0 0.0 0.0 0.024843422268506974 0.0 32-33 0.0 0.0 0.0 0.05353711662290801 0.0 34-35 0.0 0.0 0.0 0.09576787870787314 0.0 36-37 0.0 0.0 0.0 0.1602752150040825 0.0 38-39 0.0 0.0 0.0 0.2597405772230127 0.0 40-41 0.0 0.0 0.0 0.36828158078726453 0.0 42-43 0.0 0.0 0.0 0.47092494536280566 0.0 44-45 0.0 0.0 0.0 0.5969144041734504 0.0 46-47 0.0 0.0 0.0 0.7416357422591197 0.0 48-49 0.0 0.0 0.0 0.8949132405356646 0.0 50-51 0.0 0.0 0.0 1.0627667688516655 0.0 52-53 0.0 0.0 0.0 1.2193750580596583 0.0 54-55 0.0 0.0 0.0 1.385853489202126 0.0 56-57 0.0 0.0 0.0 1.5746696099857722 0.0 58-59 0.0 0.0 0.0 1.7772061447897873 0.0 60-61 0.0 0.0 0.0 2.0020498115200143 0.0 62-63 0.0 0.0 0.0 2.2213930895561065 0.0 64-65 0.0 0.0 0.0 2.4376194806655227 0.0 66-67 0.0 0.0 0.0 2.6682691132395577 0.0 68-69 0.0 0.0 0.0 2.928865306481658 0.0 70-71 0.0 0.0 0.0 3.214427152852135 0.0 72-73 0.0 0.0 0.0 3.485901892622634 0.0 74-75 0.0 0.0 0.0 3.7552070346304474 0.0 76-77 0.0 0.0 0.0 4.05286973612802 0.0 78-79 0.0 0.0 0.0 4.378217727386069 0.0 80-81 0.0 0.0 0.0 4.734551241621075 0.0 82-83 0.0 0.0 0.0 5.088592927233524 0.0 84-85 0.0 0.0 0.0 5.437317570441644 0.0 86-87 0.0 0.0 0.0 5.791542602343899 0.0 88-89 0.0 0.0 0.0 6.169235959341126 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 9635 0.0 44.026344 1 GTATCAA 15610 0.0 36.15151 1 TATCAAC 19600 0.0 28.743624 2 ATCAACG 20035 0.0 28.140673 3 TCAACGC 20350 0.0 27.728418 4 CAACGCA 20670 0.0 27.299147 5 AACGCAG 20955 0.0 26.973192 6 GTACATG 12580 0.0 24.657295 1 CGATCGT 155 0.0 24.517965 24-25 GAGTACT 13565 0.0 24.440422 12-13 ACGCAGA 23405 0.0 24.088799 7 TACATGG 12750 0.0 23.955965 2 CGCAGAG 23525 0.0 23.925543 8 GCAGAGT 24035 0.0 23.141197 9 ACATGGG 12900 0.0 22.717918 3 GTACTTT 14360 0.0 22.70697 14-15 AGTACTT 13835 0.0 22.45286 12-13 CATGGGA 8390 0.0 20.83334 4 AGAGTAC 21755 0.0 20.57763 10-11 ACTTTTT 16050 0.0 20.390001 16-17 >>END_MODULE