FastQCFastQC Report
Thu 26 May 2016
SRR937933_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937933_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1644304
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT241511.4687673325613755No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT180841.0997966312798606No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT128900.7839183022117564No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA51770.31484445698605606No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39910.24271667526199533No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37850.2301885782677656No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25830.15708774046648308No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24190.14711391567496035No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20990.12765279413052574No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20490.12461199388920784No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA90050.045.729881
GTATCAA150300.038.0338971
TATCAAC187550.030.3780612
ATCAACG189400.030.0562483
TCAACGC191450.029.7840544
CAACGCA193850.029.3907955
AACGCAG197750.028.7871236
ACGCAGA219150.025.817537
CGCAGAG220150.025.6138698
GAGTACT125050.025.20419912-13
GTACATG128050.025.1906931
GGAACGC1701.1130942E-725.1711358
TACATGG128700.024.5528012
GCAGAGT228150.024.4023919
GTACTTT132500.023.6087114-15
AGTACTT126400.023.52465812-13
ACATGGG131900.022.6602023
CATGGGA88900.021.3269654
TAGACCG1353.7872148E-421.1190995
ACTTTTT149150.021.09964616-17
Produced by FastQC (version 0.11.5)