##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937933_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1644304 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.36918477361851 33.0 31.0 34.0 31.0 34.0 2 32.54563207290136 34.0 31.0 34.0 31.0 34.0 3 32.59658919518532 34.0 31.0 34.0 31.0 34.0 4 36.10172084967257 37.0 37.0 37.0 35.0 37.0 5 36.141709197326044 37.0 37.0 37.0 35.0 37.0 6 36.20765928927984 37.0 37.0 37.0 35.0 37.0 7 36.17393863908377 37.0 37.0 37.0 35.0 37.0 8 36.175380586558205 37.0 37.0 37.0 35.0 37.0 9 37.910381535287875 39.0 38.0 39.0 35.0 39.0 10-11 37.854609305821796 39.0 38.0 39.0 35.0 39.0 12-13 37.772213045762825 39.0 38.0 39.0 35.0 39.0 14-15 39.069889144586405 40.5 39.0 41.0 36.0 41.0 16-17 39.03328095048118 40.0 39.0 41.0 36.0 41.0 18-19 39.05877197890415 40.0 39.0 41.0 36.0 41.0 20-21 39.01121477537001 40.0 39.0 41.0 36.0 41.0 22-23 38.87503375288268 40.0 38.5 41.0 35.0 41.0 24-25 38.82726734229193 40.0 38.5 41.0 35.0 41.0 26-27 38.81786093082545 40.0 38.0 41.0 35.0 41.0 28-29 38.724640030067434 40.0 38.0 41.0 35.0 41.0 30-31 38.48301621841216 40.0 38.0 41.0 35.0 41.0 32-33 38.19252066527844 40.0 37.5 41.0 35.0 41.0 34-35 37.985780913991576 40.0 37.0 41.0 34.0 41.0 36-37 37.785104214305875 40.0 37.0 41.0 34.0 41.0 38-39 37.574936872986996 40.0 36.5 41.0 33.5 41.0 40-41 37.34884334040421 40.0 36.0 41.0 33.0 41.0 42-43 37.04308996389962 40.0 36.0 41.0 33.0 41.0 44-45 36.70395437826582 40.0 35.5 41.0 31.5 41.0 46-47 36.378432151232374 40.0 36.0 41.0 30.5 41.0 48-49 36.02048222226547 40.0 35.0 41.0 29.5 41.0 50-51 35.33917237931672 38.5 35.0 40.5 28.0 41.0 52-53 35.39921115560139 39.0 35.0 40.0 28.0 41.0 54-55 35.54967603314229 39.0 35.0 41.0 28.0 41.0 56-57 35.38603111103543 39.0 35.0 41.0 28.0 41.0 58-59 35.17238570240053 39.0 35.0 41.0 27.0 41.0 60-61 34.85889926680225 38.5 35.0 40.5 26.0 41.0 62-63 34.50442406027109 38.0 35.0 40.0 26.0 41.0 64-65 34.135379467543714 37.0 34.5 40.0 24.5 41.0 66-67 33.74915283305277 37.0 34.0 40.0 23.0 41.0 68-69 33.318341985423615 36.0 34.0 39.0 21.5 41.0 70-71 32.88596299711002 36.0 33.5 39.0 20.0 40.5 72-73 32.43913838317002 35.0 33.0 38.5 18.5 40.0 74-75 31.958502199106732 35.0 33.0 37.5 15.0 39.5 76-77 31.46829752892409 35.0 32.0 37.0 9.0 39.0 78-79 30.95457470151505 35.0 31.5 36.0 6.0 39.0 80-81 30.474293378839924 35.0 31.0 36.0 2.0 37.5 82-83 30.004121804727106 34.5 30.5 35.0 2.0 37.0 84-85 29.55300844612675 34.0 30.0 35.0 2.0 37.0 86-87 29.111970779126 34.0 29.5 35.0 2.0 36.0 88-89 28.638518485632826 34.0 29.0 35.0 2.0 36.0 90-91 28.118304461948643 34.0 28.5 35.0 2.0 35.0 92-93 27.596573383024065 34.0 27.0 35.0 2.0 35.0 94-95 27.120850828070722 33.0 26.5 35.0 2.0 35.0 96-97 26.424003712208936 33.0 25.0 35.0 2.0 35.0 98-99 25.52928716344423 33.0 22.0 35.0 2.0 35.0 100-101 22.798402241921202 29.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.28073527297615897 1101 2 0.2196540483722842 1101 3 0.18843236388588736 1101 4 0.020676413554525652 1101 5 0.08046090094384795 1101 6 0.09530178215280216 1101 7 0.1639923340215148 1101 8 0.1266583256322491 1101 9 0.12240368734109097 1101 10-11 0.0821681973404651 1101 12-13 0.09697191741773281 1101 14-15 0.1550484231917011 1101 16-17 0.21895102218287832 1101 18-19 0.1845163872061022 1101 20-21 0.20082741735356535 1101 22-23 0.21503075973551944 1101 24-25 0.25794401419878454 1101 26-27 0.17030881595709957 1101 28-29 0.2123089335986208 1101 30-31 0.20290497373444083 1101 32-33 0.23490268902417455 1101 34-35 0.27855149003905666 1101 36-37 0.27228486180559486 1101 38-39 0.25320564218031194 1101 40-41 0.2791133377519017 1101 42-43 0.37797240331476445 1101 44-45 0.3552720833951568 1101 46-47 0.2559799457941878 1101 48-49 0.2038501506406547 1101 50-51 0.2768006230727522 1101 52-53 0.27392638146814363 1101 54-55 0.26962142811898815 1101 56-57 0.2410098668739522 1101 58-59 0.15707180878833782 1101 60-61 0.18112141242531266 1101 62-63 0.19342074481446758 1101 64-65 0.08314442954485202 1101 66-67 0.1037049543601114 1101 68-69 0.1585472089727702 1101 70-71 0.2704395312056036 1101 72-73 0.250309855299534 1101 74-75 0.2712271849498826 1101 76-77 0.389838970196525 1101 78-79 0.18700099786229885 1101 80-81 0.05425516231412075 1101 82-83 0.2581570091824048 1101 84-85 0.387401383486214 1101 86-87 0.2629727333865901 1101 88-89 0.11926835876719366 1101 90-91 0.34582428311035684 1101 92-93 0.5413032201421544 1101 94-95 0.7954704050087749 1101 96-97 0.7533706819949906 1101 98-99 0.8214791828482539 1101 100-101 0.8453914155166338 1102 1 0.37538695478709627 1102 2 0.2539212036677654 1102 3 0.29059393738530304 1102 4 0.19328839248330354 1102 5 0.06894552471936777 1102 6 0.14901512827502472 1102 7 0.1190252286994351 1102 8 0.15735480080586228 1102 9 0.19786307544455894 1102 10-11 0.2006859844681088 1102 12-13 0.16062800011206946 1102 14-15 0.27241516526626697 1102 16-17 0.2348370559606039 1102 18-19 0.2068138829834396 1102 20-21 0.2587759644530436 1102 22-23 0.33146475454474 1102 24-25 0.18148336690889977 1102 26-27 0.21796176770142495 1102 28-29 0.24849016867601392 1102 30-31 0.2542219698852364 1102 32-33 0.3287926794772815 1102 34-35 0.3652325039080182 1102 36-37 0.3150674219841392 1102 38-39 0.4545375497492117 1102 40-41 0.3131418207994514 1102 42-43 0.4672532878618867 1102 44-45 0.4829603926642392 1102 46-47 0.36732166892721807 1102 48-49 0.17586012024464281 1102 50-51 0.3313936533057671 1102 52-53 0.2622406210216468 1102 54-55 0.28549469987218856 1102 56-57 0.3302929903727332 1102 58-59 0.19237649428727366 1102 60-61 0.1354725463199955 1102 62-63 0.004097230627571946 1102 64-65 -0.09315913289592714 1102 66-67 0.03104380805267226 1102 68-69 0.10837476491521159 1102 70-71 -0.008388762695318519 1102 72-73 4.616562226971155E-4 1102 74-75 0.10762877765053602 1102 76-77 -0.23321179848036167 1102 78-79 -0.022395613618687804 1102 80-81 0.0979803211453536 1102 82-83 -0.03172566079432926 1102 84-85 0.014295088807934064 1102 86-87 -0.17509159438160182 1102 88-89 -0.03300655046570711 1102 90-91 0.17111373478213565 1102 92-93 0.3515690822191466 1102 94-95 0.3234230449119089 1102 96-97 0.46189074389752705 1102 98-99 0.6301115057874789 1102 100-101 0.8690392230456006 1103 1 0.3520603827789799 1103 2 0.3723266957884164 1103 3 0.27873545082480433 1103 4 0.16067111809927326 1103 5 0.1567777560664254 1103 6 0.17286462674676528 1103 7 0.20121626947926075 1103 8 0.22109654012933788 1103 9 0.22684096734607806 1103 10-11 0.25229637850733866 1103 12-13 0.2240394811968187 1103 14-15 0.22707440207217644 1103 16-17 0.2880839692594819 1103 18-19 0.3350591713695792 1103 20-21 0.39242166088221353 1103 22-23 0.3639265015665032 1103 24-25 0.325975039336619 1103 26-27 0.23897062326482654 1103 28-29 0.29196270427426185 1103 30-31 0.2537374504500107 1103 32-33 0.4063586277214952 1103 34-35 0.34977536319733105 1103 36-37 0.314597885510544 1103 38-39 0.13549078687083238 1103 40-41 0.1396403912982933 1103 42-43 0.1038858693459801 1103 44-45 0.14401456852115757 1103 46-47 0.05324967136500902 1103 48-49 0.08984990144275429 1103 50-51 0.11210028354062018 1103 52-53 0.1728727416213971 1103 54-55 0.147101725910467 1103 56-57 -0.01197272042318076 1103 58-59 0.13784463375797174 1103 60-61 0.0790709810014576 1103 62-63 0.07220154411670876 1103 64-65 0.10226258049647896 1103 66-67 0.05154472011409439 1103 68-69 0.08506887361469495 1103 70-71 0.22734387036393144 1103 72-73 0.2756657410423031 1103 74-75 0.04137327721539563 1103 76-77 -0.012128914424849313 1103 78-79 0.25158218965122714 1103 80-81 0.142887844617956 1103 82-83 0.22208950770279046 1103 84-85 0.14756009139513537 1103 86-87 0.0821329364828749 1103 88-89 0.02252895746513417 1103 90-91 0.12225872848929598 1103 92-93 0.1894669431586955 1103 94-95 0.3324178865525589 1103 96-97 0.24188811027272195 1103 98-99 0.3774396849820114 1103 100-101 0.6015980005709913 1104 1 0.11557703326646873 1104 2 0.07002456248134337 1104 3 -0.06106095724071281 1104 4 0.036796430935851276 1104 5 0.027849224276287998 1104 6 -0.011857067893473072 1104 7 0.004467268758617138 1104 8 0.06157372435784225 1104 9 -0.052513303566882996 1104 10-11 0.09456773229052828 1104 12-13 -0.0022251914444169074 1104 14-15 -7.706717189535084E-4 1104 16-17 0.011453584601404998 1104 18-19 0.08494818375173452 1104 20-21 0.16369142175860674 1104 22-23 0.13891200283191552 1104 24-25 0.21649005314754532 1104 26-27 0.1487587932353307 1104 28-29 0.2502157279052426 1104 30-31 0.0833101012251305 1104 32-33 0.08635787738267453 1104 34-35 0.055130984720172194 1104 36-37 0.10776308041887006 1104 38-39 0.14701122344297346 1104 40-41 0.06978032948914148 1104 42-43 0.015687057695096485 1104 44-45 9.335522497480042E-4 1104 46-47 -0.033895930320746004 1104 48-49 -0.07876139334504018 1104 50-51 0.08988048428752649 1104 52-53 0.04073924696521658 1104 54-55 0.1020516955061126 1104 56-57 0.042342822063965 1104 58-59 0.07216536608866164 1104 60-61 0.14091922184771022 1104 62-63 -0.0014590620007339794 1104 64-65 0.15798031866261653 1104 66-67 0.2729970634378702 1104 68-69 0.2479080306715602 1104 70-71 0.04786321425515894 1104 72-73 0.10795133563110682 1104 74-75 -0.009394960316864598 1104 76-77 0.030270700401221262 1104 78-79 0.052377001683534985 1104 80-81 0.2626341050386749 1104 82-83 0.2983235415086831 1104 84-85 0.3183856673192622 1104 86-87 0.24993839863687484 1104 88-89 0.16883291347026486 1104 90-91 0.22048511509199287 1104 92-93 0.0480069713901905 1104 94-95 -0.0040070772201481475 1104 96-97 -0.007462377681996202 1104 98-99 0.041132352452162735 1104 100-101 0.07521382083983141 1105 1 0.1775292273040847 1105 2 0.19335916201144698 1105 3 0.15347498164419449 1105 4 0.09015959023747655 1105 5 -0.0044106555757110755 1105 6 0.04683115225594037 1105 7 0.0621425314944517 1105 8 0.13193205804289931 1105 9 0.20119735640300718 1105 10-11 0.17514708765568798 1105 12-13 0.03957688793204994 1105 14-15 0.15432788059607816 1105 16-17 0.08878303493132478 1105 18-19 0.09334377175944297 1105 20-21 0.2067491462623252 1105 22-23 0.18033774039153627 1105 24-25 0.1873886307865078 1105 26-27 0.18396961499703224 1105 28-29 0.22854265991047384 1105 30-31 0.194014388004625 1105 32-33 0.35488655673943725 1105 34-35 0.21253331170844803 1105 36-37 0.19984020587083506 1105 38-39 0.3638298337216739 1105 40-41 0.4280049809333022 1105 42-43 0.35580157815824265 1105 44-45 0.4270017299112254 1105 46-47 0.6368014627207899 1105 48-49 0.522161523409558 1105 50-51 0.5100467781295848 1105 52-53 0.42549423789080265 1105 54-55 0.3535781660529693 1105 56-57 0.49605981301769475 1105 58-59 0.5078285014732344 1105 60-61 0.5988498342648825 1105 62-63 0.534609959791915 1105 64-65 0.6288472413824095 1105 66-67 0.5974881226818098 1105 68-69 0.6275841656762751 1105 70-71 0.6670158472409966 1105 72-73 0.7488122087441269 1105 74-75 0.5651347882727329 1105 76-77 0.742643553142539 1105 78-79 0.7966685645924123 1105 80-81 0.862354134864642 1105 82-83 0.8007680047115961 1105 84-85 0.9683068817426488 1105 86-87 1.0556827113030671 1105 88-89 0.9897434496342541 1105 90-91 0.9845293475657222 1105 92-93 0.968454642291583 1105 94-95 0.9528050613861936 1105 96-97 0.9588673399561287 1105 98-99 0.8571398933573562 1105 100-101 0.7450072900782843 1106 1 -0.10022801847493668 1106 2 -0.05616511891029319 1106 3 -0.12756665064971173 1106 4 -0.015536939488093537 1106 5 -0.023545011842550423 1106 6 -0.01571078251830471 1106 7 0.03774941704688928 1106 8 -0.0777935097951925 1106 9 -0.044024004208552014 1106 10-11 -0.3962883190428812 1106 12-13 -0.28013328090698053 1106 14-15 -0.16887126145968523 1106 16-17 -0.07550943605806992 1106 18-19 -0.06846341077368834 1106 20-21 -0.04220042201573193 1106 22-23 -0.17287020109226603 1106 24-25 -0.04952826961496015 1106 26-27 -0.10477371668640956 1106 28-29 -0.07454373947356174 1106 30-31 -0.03957962838430973 1106 32-33 -0.12105690693542215 1106 34-35 -0.26376927862317956 1106 36-37 -0.26106448532423343 1106 38-39 -0.33018435535603174 1106 40-41 -0.07781694064178879 1106 42-43 -0.12648682309568215 1106 44-45 -0.080608675412428 1106 46-47 -0.012233899980635954 1106 48-49 -0.11864307429159737 1106 50-51 -0.1659549817560233 1106 52-53 -0.22844735590559395 1106 54-55 -0.38141330950112007 1106 56-57 -0.39018008138916827 1106 58-59 -0.1457638650229498 1106 60-61 -0.4891015488952064 1106 62-63 -0.2786216301547313 1106 64-65 -0.16975529945244006 1106 66-67 -0.26580231343984195 1106 68-69 -0.6603798666616285 1106 70-71 -0.5098290078434289 1106 72-73 -0.3914270257056387 1106 74-75 -0.2181297995290592 1106 76-77 -0.2499577777709554 1106 78-79 -0.10100343672750256 1106 80-81 -0.30121293327592724 1106 82-83 -0.2986094357547735 1106 84-85 -0.29765528071678915 1106 86-87 -0.2300033691017056 1106 88-89 -0.3866234865148961 1106 90-91 -0.2897719228517026 1106 92-93 -0.11559749431953392 1106 94-95 -0.09729998178600141 1106 96-97 0.026907267360847698 1106 98-99 -0.0011717163880113901 1106 100-101 -0.19691703484059886 1107 1 0.24022521553860088 1107 2 0.1287707953389443 1107 3 0.16684206431295934 1107 4 0.12246232610493735 1107 5 0.1401815789353975 1107 6 0.12156386820124254 1107 7 0.08520294832038644 1107 8 0.14092897180091768 1107 9 0.1352653696220827 1107 10-11 0.07387255105452084 1107 12-13 -0.024523460836682887 1107 14-15 0.0970643205578341 1107 16-17 0.0728388477027977 1107 18-19 0.24174694436453592 1107 20-21 0.1666150844627836 1107 22-23 0.26745993232499643 1107 24-25 0.2102835823146023 1107 26-27 0.2517941547573983 1107 28-29 0.20544297125752564 1107 30-31 0.23717848943555708 1107 32-33 0.32161808301020045 1107 34-35 0.41361850574094916 1107 36-37 0.4527579280769416 1107 38-39 0.4470906450811114 1107 40-41 0.2991328224245322 1107 42-43 0.5187307886828094 1107 44-45 0.3694086356089201 1107 46-47 0.2585879363865047 1107 48-49 0.30715996475437635 1107 50-51 0.3177467790721167 1107 52-53 0.20473839659925375 1107 54-55 0.3626904517116998 1107 56-57 0.42300077506924083 1107 58-59 0.35129693338234347 1107 60-61 0.24581705433268297 1107 62-63 0.2792858345675029 1107 64-65 0.190308174292511 1107 66-67 0.1462162565648839 1107 68-69 0.15542649470157244 1107 70-71 0.30060324311392606 1107 72-73 0.28104512803021464 1107 74-75 0.3703714856831333 1107 76-77 0.4605461652950176 1107 78-79 0.33017581102993177 1107 80-81 0.37175074855099766 1107 82-83 0.4130630886817457 1107 84-85 0.29760202400964175 1107 86-87 0.20399250423058035 1107 88-89 0.3271998011802886 1107 90-91 0.33926443029313447 1107 92-93 0.4829539753686909 1107 94-95 0.5972300851849219 1107 96-97 0.6171149996170477 1107 98-99 0.7225240992408679 1107 100-101 0.7007701077917297 1108 1 0.22204998194691683 1108 2 0.0865860587596714 1108 3 0.006148458680250712 1108 4 -0.01031441259502941 1108 5 0.093537882061689 1108 6 0.0450499571287466 1108 7 -0.011397035881465456 1108 8 0.017343943625320435 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-0.3992135820261069 2316 62-63 -0.34337847962856927 2316 64-65 -0.31364617145651863 2316 66-67 -0.4850888555565689 2316 68-69 -0.39488375305230505 2316 70-71 -0.5729883421348418 2316 72-73 -0.434839168551747 2316 74-75 -0.3747660240898121 2316 76-77 -0.32679442573967776 2316 78-79 -0.21652010262153 2316 80-81 0.025074520558696634 2316 82-83 -0.15025779079935475 2316 84-85 -0.11464892959723016 2316 86-87 -0.2668285819646563 2316 88-89 -0.2862761065448751 2316 90-91 -0.36853785859877064 2316 92-93 -0.3858210969959721 2316 94-95 -0.4069802624096788 2316 96-97 -0.34784540984465906 2316 98-99 -0.46617133006687084 2316 100-101 -0.38495328721647226 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4527.0 3 734.0 4 643.0 5 643.0 6 745.0 7 854.0 8 966.0 9 1226.0 10 1640.0 11 2286.0 12 3850.0 13 5820.0 14 6890.0 15 9501.0 16 14256.0 17 24264.0 18 20255.0 19 10766.0 20 9203.0 21 8725.0 22 8975.0 23 9320.0 24 10255.0 25 11216.0 26 13182.0 27 15367.0 28 19013.0 29 23302.0 30 28787.0 31 36886.0 32 48591.0 33 67216.0 34 99323.0 35 165201.0 36 239587.0 37 353984.0 38 321422.0 39 44878.0 40 5.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.090637579217756 19.215973330530822 14.967298616423525 20.726090473827895 2 15.267380866763167 20.995280243526533 41.42695207898671 22.31038681072359 3 18.79642424895172 27.130472562116537 30.621217072136293 23.451886116795453 4 11.949669077286702 17.545050033956393 37.80070882452455 32.70457206423236 5 12.347373379702043 39.010822476571185 33.40160018307282 15.24020396065395 6 29.194695836077912 37.29905748937377 17.85802558573608 15.648221088812237 7 26.704589023005365 35.76158940397351 20.365729420112928 17.168092152908198 8 26.316868697071214 34.61449532151858 19.51247412505887 19.55616185635133 9 27.80040547315748 16.614664206893533 20.501706102541387 35.0832242174076 10-11 24.676600353167537 27.795952784001503 26.236946022818042 21.290500840012914 12-13 26.374741604800466 25.909713954877983 27.454895179291487 20.26064926103006 14-15 23.1457590032058 26.135697415494498 27.73257248489971 22.985971096399997 16-17 20.282576841818305 29.93383741760863 28.0143132186426 21.769272521930468 18-19 20.089364493465308 27.119592401523203 32.64782625098729 20.1432168540242 20-21 21.783333936852063 25.846587162456796 32.799670630922535 19.57040826976861 22-23 21.102865848333888 26.2342462623239 32.566167904166036 20.096719985176176 24-25 20.480664177219428 26.419304476440033 33.00045345677096 20.09957788956958 26-27 20.307058767328652 26.61193225574518 32.97869459555757 20.102314381368597 28-29 19.968684210446245 26.872915158613015 33.09876918480059 20.05963144614016 30-31 20.17916902233877 26.70562324541732 32.98490686144145 20.13030087080247 32-33 20.049871745328517 26.86252255486957 32.7503704627818 20.33723523702011 34-35 20.461086945607075 26.940586331706918 32.57580944295597 20.02251727973004 36-37 20.553051198921647 26.715939017098762 32.56323225807433 20.167777525905258 38-39 20.250730221655203 26.97299776902911 32.50211872269464 20.27415328662105 40-41 20.326719842298356 26.924604985367573 32.46542671314971 20.283248459184357 42-43 20.35121405732333 26.891083000763853 32.212316371909566 20.545386570003252 44-45 20.24307123793882 27.25414965365694 31.963046602340384 20.539732506063853 46-47 20.42903445196471 27.508677777953515 31.36361258359927 20.6986751864825 48-49 20.621785202968496 27.499880616608372 31.12849698286993 20.7498371975532 50-51 20.628574400607825 27.94564979686957 30.619209444401697 20.806566358120914 52-53 20.729079729522574 28.091444290069763 30.40860559106714 20.770870389340523 54-55 20.46992225649305 27.99234639642832 30.472528812788124 21.06520253429051 56-57 20.590670303519897 28.218937510037033 30.3091041369611 20.881288049481967 58-59 20.708362999768546 27.832960811107966 30.445807064408275 21.012869124715213 60-61 20.724906356503794 27.922019262501387 30.615545352651598 20.73752902834322 62-63 20.62242016737206 27.895373606601453 30.62955519518833 20.852651030838164 64-65 20.53975042633246 27.80253332875091 30.59921950821901 21.05849673669762 66-67 20.50557218784252 28.03489901207865 30.393909460615138 21.065619339463694 68-69 20.44869502169084 28.014941496777396 30.43666956202266 21.099693919509104 70-71 20.535562455567273 28.109621561701754 30.253860874956235 21.10095510777474 72-73 20.666903934076164 28.092461284346737 30.061233242989204 21.179401538587893 74-75 20.44988605628199 28.426463413298748 29.80285242554724 21.320798104872022 76-77 20.423170061844985 28.19269332564453 29.97933634194248 21.404800270568003 78-79 20.54486896807608 28.205454163916876 29.94467980280585 21.304997065201192 80-81 20.524634756231453 28.580648025989653 29.66228295070859 21.232434267070307 82-83 20.6334139210427 28.41217070076635 29.573844204056716 21.380571174134236 84-85 20.633613825673017 28.20879262585355 29.492175857383206 21.665417691090223 86-87 20.60248617768048 28.131115355737137 29.61633236942276 21.65006609715963 88-89 20.705002605476157 28.00118759823771 29.590817042643113 21.702992753643027 90-91 20.718012778368834 28.140765892871865 29.5535298410903 21.587691487669005 92-93 20.817751821265077 27.91784155374063 29.52399203053946 21.740414594454837 94-95 20.75106961218325 27.929876398679742 29.518375607213887 21.80067838192312 96-97 20.809815644847564 27.825119051168123 29.469962965914892 21.89510233806942 98-99 20.851383799811533 27.82136319475158 29.230379414164137 22.09687359127275 100-101 21.195362990853233 27.398844494016117 29.445178645342995 21.96061386978765 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 5695.0 1 3605.0 2 2182.0 3 2799.5 4 2138.0 5 1911.0 6 3848.5 7 8472.0 8 13365.5 9 14138.0 10 10172.5 11 6486.5 12 5732.5 13 5709.0 14 5631.0 15 5470.0 16 5287.5 17 5191.5 18 5303.5 19 5506.0 20 5720.5 21 6282.5 22 7107.5 23 8031.5 24 9148.0 25 10560.5 26 12577.0 27 14918.0 28 16964.0 29 19193.5 30 21758.0 31 24352.0 32 27240.5 33 30269.5 34 33416.0 35 35657.5 36 38316.5 37 41479.5 38 43216.5 39 44303.0 40 45942.5 41 48407.0 42 49990.0 43 52096.5 44 54299.0 45 55863.0 46 56365.5 47 57069.5 48 61457.5 49 60924.0 50 56315.5 51 52958.5 52 49979.0 53 48597.0 54 46416.0 55 42584.5 56 37918.5 57 33685.0 58 30052.0 59 26689.0 60 22991.0 61 19406.0 62 16262.5 63 12562.0 64 9328.0 65 7266.5 66 5560.5 67 4431.5 68 3340.5 69 2174.0 70 1590.5 71 1285.5 72 1086.5 73 920.0 74 689.5 75 551.0 76 378.5 77 214.0 78 127.5 79 72.5 80 46.0 81 26.5 82 18.5 83 13.5 84 7.5 85 7.0 86 5.5 87 2.0 88 3.0 89 4.0 90 5.5 91 4.5 92 2.0 93 1.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.46682365304712514 2 0.04786219579834386 3 0.05479522034854869 4 0.0620323249228853 5 0.07553347799433681 6 0.10156272806001809 7 0.06823555741517384 8 0.0499907559672664 9 0.048166275822475645 10-11 0.07142839766855764 12-13 0.10420822426996468 14-15 0.05324441222547655 16-17 0.056437252478860354 18-19 0.056072356449902216 20-21 0.07069860561064134 22-23 0.0610288608432504 24-25 0.08325711060728429 26-27 0.06297497299769386 28-29 0.06346150103630473 30-31 0.06732331734277847 32-33 0.06622862925590402 34-35 0.06820514941276067 36-37 0.06425210909904738 38-39 0.038010003016473844 40-41 0.04220630734949255 42-43 0.04029060319746227 44-45 0.020768665648201307 46-47 0.027367202171861164 48-49 0.02678945012601076 50-51 0.02490415397639366 52-53 0.024083137911237822 54-55 0.02265396179781841 56-57 0.02383987389193239 58-59 0.020099689595111367 60-61 0.02654618610670533 62-63 0.018366433457560158 64-65 0.020707849643374947 66-67 0.014960737187284103 68-69 0.017758273409296577 70-71 0.04059468322159406 72-73 0.03582062684272495 74-75 0.03101616246144265 76-77 0.02158968171335714 78-79 0.014535025153499597 80-81 0.016116241278984908 82-83 0.020647033638548588 84-85 0.01763664139964386 86-87 0.008118936644318812 88-89 0.03956081113954597 90-91 0.05008197997450593 92-93 0.032171666553143456 94-95 0.05075095602759587 96-97 0.041537331296402615 98-99 0.03241493057244889 100-101 0.1633213809611848 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1644304.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.744318122456036 #Duplication Level Percentage of deduplicated Percentage of total 1 73.50123668495237 38.03271373420112 2 14.308022450775848 14.807177307923771 3 5.170010858115044 8.025560596165702 4 2.3874500554659903 4.941479006860301 5 1.298522120464072 3.3595570845169567 6 0.7998499553526862 2.483261432400099 7 0.5199791231933937 1.8834175617288305 8 0.364256785472457 1.507857518860003 9 0.2561239451162274 1.1927663005385285 >10 1.2719811942737136 11.716315196603775 >50 0.0790199222845542 2.8069396584922814 >100 0.03989859883406365 3.5653321034425165 >500 0.002236058331972789 0.8323331006532254 >1k 9.414982450411742E-4 1.173109807651722 >5k 1.1768728063014678E-4 0.3152610815102245 >10k+ 3.5306184189044034E-4 3.3569185084510575 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 24151 1.4687673325613755 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 18084 1.0997966312798606 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12890 0.7839183022117564 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5177 0.31484445698605606 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3991 0.24271667526199533 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3785 0.2301885782677656 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2583 0.15708774046648308 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2419 0.14711391567496035 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2099 0.12765279413052574 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2049 0.12461199388920784 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 9.122400723953722E-5 0.0 18-19 0.0 0.0 0.0 2.128560168922535E-4 0.0 20-21 0.0 0.0 0.0 3.344880265449698E-4 0.0 22-23 0.0 0.0 0.0 6.081600482635815E-4 0.0 24-25 0.0 0.0 0.0 0.0013987681110062373 0.0 26-27 0.0 0.0 0.0 0.0037097762944078468 0.0 28-29 0.0 0.0 0.0 0.00881832069982193 0.0 30-31 0.0 0.0 0.0 0.02463048195467505 0.0 32-33 0.0 0.0 0.0 0.055525012406464985 0.0 34-35 0.0 0.0 0.0 0.10049844797555682 0.0 36-37 0.0 0.0 0.0 0.16833870135935936 0.0 38-39 0.0 0.0 0.0 0.2713914215376232 0.0 40-41 0.0 0.0 0.0 0.38700264671253004 0.0 42-43 0.0 0.0 0.0 0.49172780702351876 0.0 44-45 0.0 0.0 0.0 0.6098933044011325 0.0 46-47 0.0 0.0 0.0 0.7480976753690316 0.0 48-49 0.0 0.0 0.0 0.9029048156545262 0.0 50-51 0.0 0.0 0.0 1.069449444871508 0.0 52-53 0.0 0.0 0.0 1.2272973853983205 6.0816004826358146E-5 54-55 0.0 0.0 0.0 1.3985856629917581 6.0816004826358146E-5 56-57 0.0 0.0 0.0 1.5840136617073242 6.0816004826358146E-5 58-59 0.0 0.0 0.0 1.7840983175860425 6.0816004826358146E-5 60-61 0.0 0.0 0.0 2.0131617997645206 6.0816004826358146E-5 62-63 0.0 0.0 0.0 2.2393669297161596 6.0816004826358146E-5 64-65 0.0 0.0 0.0 2.4575747550331326 6.0816004826358146E-5 66-67 0.0 0.0 0.0 2.6981628701262057 6.0816004826358146E-5 68-69 0.0 0.0 0.0 2.9624084110967317 6.0816004826358146E-5 70-71 0.0 0.0 0.0 3.2507370899784958 6.0816004826358146E-5 72-73 0.0 0.0 0.0 3.5353559925658518 6.0816004826358146E-5 74-75 0.0 0.0 0.0 3.819032247078399 6.0816004826358146E-5 76-77 0.0 0.0 0.0 4.120862079031615 6.0816004826358146E-5 78-79 0.0 0.0 0.0 4.452370121340093 6.0816004826358146E-5 80-81 0.0 0.0 0.0 4.802791941149568 6.0816004826358146E-5 82-83 0.0 0.0 0.0 5.165164105907424 6.0816004826358146E-5 84-85 0.0 0.0 0.0 5.52726259864356 6.0816004826358146E-5 86-87 0.0 0.0 0.0 5.894469635785111 6.0816004826358146E-5 88-89 0.0 0.0 0.0 6.278096994229777 6.0816004826358146E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 9005 0.0 45.72988 1 GTATCAA 15030 0.0 38.033897 1 TATCAAC 18755 0.0 30.378061 2 ATCAACG 18940 0.0 30.056248 3 TCAACGC 19145 0.0 29.784054 4 CAACGCA 19385 0.0 29.390795 5 AACGCAG 19775 0.0 28.787123 6 ACGCAGA 21915 0.0 25.81753 7 CGCAGAG 22015 0.0 25.613869 8 GAGTACT 12505 0.0 25.204199 12-13 GTACATG 12805 0.0 25.190693 1 GGAACGC 170 1.1130942E-7 25.171135 8 TACATGG 12870 0.0 24.552801 2 GCAGAGT 22815 0.0 24.402391 9 GTACTTT 13250 0.0 23.60871 14-15 AGTACTT 12640 0.0 23.524658 12-13 ACATGGG 13190 0.0 22.660202 3 CATGGGA 8890 0.0 21.326965 4 TAGACCG 135 3.7872148E-4 21.119099 5 ACTTTTT 14915 0.0 21.099646 16-17 >>END_MODULE