FastQCFastQC Report
Thu 26 May 2016
SRR937933_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937933_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1644304
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT230551.402112991271687No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT187551.140604170518347No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT133730.8132924325428874No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA51520.31332405686539716No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40390.24563584349366055No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39880.24253422724751628No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24200.1471747316797867No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23190.14103231519232454No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19990.12157119364788994No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19830.1205981375706682No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA91200.046.4631041
GTATCAA145850.036.3167231
TAGCGGA550.00258095634.54279
TATCAAC184950.028.6647452
ATCAACG188400.028.0616283
TCAACGC191100.027.6626324
CAACGCA193900.027.263175
AACGCAG196300.026.9540426
GTACATG120000.026.4047951
TACATGG121200.025.4378432
TATAACG950.001226044625.0025712
ACGCAGA220150.024.0123947
ACATGGG122750.023.9921283
GAGTACT133650.023.84583512-13
CGCAGAG221500.023.758838
TAACGCA1401.7811846E-523.7481064
TAGCCCT8950.023.3500944
AGTACTT135900.022.82194912-13
GCAGAGT230300.022.5622489
GTACTTT140650.022.54086114-15
Produced by FastQC (version 0.11.5)