##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937933_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1644304 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.19940838190505 33.0 31.0 34.0 31.0 34.0 2 32.431157498856656 34.0 31.0 34.0 31.0 34.0 3 32.466368141170975 34.0 31.0 34.0 30.0 34.0 4 36.043614806021274 37.0 35.0 37.0 35.0 37.0 5 36.00900441767459 37.0 35.0 37.0 35.0 37.0 6 36.070665764968034 37.0 35.0 37.0 35.0 37.0 7 36.02387210637449 37.0 35.0 37.0 35.0 37.0 8 36.02834025824908 37.0 35.0 37.0 35.0 37.0 9 37.64986705621345 39.0 37.0 39.0 35.0 39.0 10-11 37.62667517685294 39.0 37.0 39.0 35.0 39.0 12-13 37.56295672819625 39.0 37.0 39.0 35.0 39.0 14-15 38.6842943883856 40.0 38.0 41.0 34.5 41.0 16-17 38.63683935573957 40.0 38.0 41.0 34.5 41.0 18-19 38.730903166324474 40.0 38.0 41.0 35.0 41.0 20-21 38.69448532631436 40.0 38.0 41.0 34.0 41.0 22-23 38.51307908999796 40.0 38.0 41.0 34.0 41.0 24-25 38.43977421450048 40.0 38.0 41.0 34.0 41.0 26-27 38.362839535754944 40.0 38.0 41.0 34.0 41.0 28-29 38.2694267605017 40.0 38.0 41.0 34.0 41.0 30-31 37.96953391830221 40.0 37.0 41.0 33.5 41.0 32-33 37.685955881637454 40.0 36.0 41.0 33.0 41.0 34-35 37.42146221136724 39.5 36.0 41.0 33.0 41.0 36-37 37.24084019743308 39.0 35.0 41.0 33.0 41.0 38-39 37.17689064795805 39.0 35.0 41.0 33.0 41.0 40-41 37.05977179402349 40.0 35.0 41.0 32.0 41.0 42-43 36.81980704297989 39.0 35.0 41.0 31.5 41.0 44-45 36.46723142435948 39.0 35.0 41.0 30.5 41.0 46-47 35.92724398894608 39.0 35.0 41.0 29.5 41.0 48-49 35.41051350601835 39.0 35.0 40.0 27.0 41.0 50-51 35.033549149062466 38.5 35.0 40.0 26.0 41.0 52-53 34.55722117078108 38.0 34.0 40.0 24.0 41.0 54-55 34.30563660977532 38.0 34.0 40.0 23.5 41.0 56-57 34.0476700172231 38.0 34.0 40.0 23.0 41.0 58-59 33.69928979069564 37.5 33.0 40.0 22.0 41.0 60-61 33.30670362658 37.0 33.0 40.0 20.5 41.0 62-63 32.918968451089334 36.0 32.5 39.0 19.5 41.0 64-65 32.50547891387481 36.0 32.0 39.0 17.5 40.0 66-67 32.04882035195438 35.0 31.0 39.0 13.5 40.0 68-69 31.57418153820705 35.0 31.0 38.0 9.5 40.0 70-71 31.025593199311075 35.0 30.5 37.5 7.5 39.5 72-73 30.582984654905662 34.5 30.0 37.0 7.0 39.0 74-75 30.106442604287285 34.0 29.0 36.0 3.5 39.0 76-77 28.887218239449638 32.5 28.0 35.0 2.0 37.5 78-79 29.668212508149345 34.0 29.0 36.0 2.0 38.0 80-81 29.497090562329106 34.0 29.0 35.0 2.0 37.0 82-83 29.089171467076646 34.0 29.0 35.0 2.0 37.0 84-85 28.644909335500003 34.0 29.0 35.0 2.0 36.0 86-87 28.136790094775662 33.5 27.5 35.0 2.0 36.0 88-89 27.559823183547568 33.0 26.5 35.0 2.0 35.0 90-91 27.10337565316389 33.0 26.0 35.0 2.0 35.0 92-93 26.573305787737546 32.5 25.0 35.0 2.0 35.0 94-95 26.026349446331093 32.0 24.5 34.0 2.0 35.0 96-97 25.266257638490206 32.0 21.5 34.0 2.0 35.0 98-99 24.362755609668284 31.5 10.0 34.0 2.0 35.0 100-101 21.873842975508175 28.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.14799263273207686 1101 2 0.14550125100299738 1101 3 0.2193290044971974 1101 4 0.04788594324420359 1101 5 0.07018066518192967 1101 6 0.09151389622505235 1101 7 0.13929997670159366 1101 8 0.15274771448039104 1101 9 0.1765700427368273 1101 10-11 0.11838818356905279 1101 12-13 0.23130836200824945 1101 14-15 0.2528129760194915 1101 16-17 0.3003965646019182 1101 18-19 0.25627431509187204 1101 20-21 0.19247016817718787 1101 22-23 0.2631576112001426 1101 24-25 0.1808135510177422 1101 26-27 0.18233494818971963 1101 28-29 0.2528071144347237 1101 30-31 0.2647791313662893 1101 32-33 0.36026512085154394 1101 34-35 0.05069030198454527 1101 36-37 0.20232580227295927 1101 38-39 0.32087022749084326 1101 40-41 0.38377159248221204 1101 42-43 0.4896254406885845 1101 44-45 0.33963540041742846 1101 46-47 0.31143258315334066 1101 48-49 0.5232873868619095 1101 50-51 0.517850961843429 1101 52-53 0.4172108783613382 1101 54-55 0.3653401011745814 1101 56-57 0.5205651003340535 1101 58-59 0.7133153405225627 1101 60-61 0.6922723358632794 1101 62-63 0.532417033013374 1101 64-65 0.6026374744688923 1101 66-67 0.5533605568896149 1101 68-69 0.6592498761437682 1101 70-71 0.6568240985018647 1101 72-73 0.3410843176094609 1101 74-75 0.5661547230348454 1101 76-77 0.3039120129020425 1101 78-79 0.48884061418116076 1101 80-81 0.599239822826366 1101 82-83 0.7435116063416842 1101 84-85 0.7636627855020741 1101 86-87 0.6867960077547615 1101 88-89 0.7697313553189993 1101 90-91 0.6930901672869254 1101 92-93 0.747635031756257 1101 94-95 0.5377386538856115 1101 96-97 0.4503592175650084 1101 98-99 0.5479812272415998 1101 100-101 0.7893511260441244 1102 1 0.27843753929614223 1102 2 0.2439748233130814 1102 3 0.21070680161951572 1102 4 0.1225979692805268 1102 5 0.14073003227507286 1102 6 0.16589097509167772 1102 7 0.17342607804192056 1102 8 0.2307093549692354 1102 9 0.3101732145253209 1102 10-11 0.22878932310590017 1102 12-13 0.24961268748375431 1102 14-15 0.3175710707253572 1102 16-17 0.35586009924387696 1102 18-19 0.3959965417919946 1102 20-21 0.36959139576752875 1102 22-23 0.4247622320381481 1102 24-25 0.3522426917797432 1102 26-27 0.3185666492377237 1102 28-29 0.407092154476139 1102 30-31 0.26652215524068623 1102 32-33 0.2350873480890172 1102 34-35 0.4356372925648344 1102 36-37 0.3990147957656731 1102 38-39 0.2490001028080968 1102 40-41 0.5073283642363151 1102 42-43 0.4199485930608944 1102 44-45 0.41258962549834166 1102 46-47 0.34437875696405484 1102 48-49 0.33358325089127305 1102 50-51 0.3506520963650175 1102 52-53 0.34001182481098624 1102 54-55 0.34077992084046116 1102 56-57 0.31537704630546415 1102 58-59 0.35046187722256406 1102 60-61 0.3594208249291313 1102 62-63 0.4364981045577281 1102 64-65 0.5505193814108935 1102 66-67 0.32493845360050955 1102 68-69 0.5552649004050494 1102 70-71 0.6243566125580031 1102 72-73 0.6507846204948429 1102 74-75 0.6727724820412533 1102 76-77 0.4715414287864199 1102 78-79 0.5392150116792642 1102 80-81 0.44361208346900227 1102 82-83 0.6709563624482016 1102 84-85 0.47975923595765835 1102 86-87 0.21098631327314976 1102 88-89 0.05101107740240707 1102 90-91 0.11828141853850127 1102 92-93 0.42043709768174153 1102 94-95 0.47357723979956035 1102 96-97 0.46026926544274716 1102 98-99 0.566520642642363 1102 100-101 0.26149185042559253 1103 1 0.22770480916418734 1103 2 0.2090155643070517 1103 3 0.2542244971302807 1103 4 0.15284498134032987 1103 5 0.17303709604508555 1103 6 0.13127653523075367 1103 7 0.13515706170922925 1103 8 0.06964800417294015 1103 9 0.21867669943558354 1103 10-11 0.13242503370766912 1103 12-13 0.20544629304272632 1103 14-15 0.36143210818782023 1103 16-17 0.297487957929647 1103 18-19 0.20279800446669327 1103 20-21 0.17361678998823038 1103 22-23 0.24704151613114078 1103 24-25 0.2809482736293347 1103 26-27 0.2993255721189456 1103 28-29 0.3563760955268336 1103 30-31 0.4057277966000683 1103 32-33 0.422957859847898 1103 34-35 0.3711354317232036 1103 36-37 0.5274550736806205 1103 38-39 0.4611647482521377 1103 40-41 0.5466107352414511 1103 42-43 0.5124776663372757 1103 44-45 0.6228527009812339 1103 46-47 0.6398520455088814 1103 48-49 0.678381926313051 1103 50-51 0.7940673590648686 1103 52-53 0.847637941252664 1103 54-55 0.835743957511994 1103 56-57 0.7224660130213394 1103 58-59 0.5252176549480012 1103 60-61 0.5221558358477125 1103 62-63 0.6682376728289654 1103 64-65 0.5576455734004497 1103 66-67 0.43865554489696734 1103 68-69 0.5795299122774651 1103 70-71 0.5533913975081894 1103 72-73 0.34676914457267216 1103 74-75 0.24920225753318093 1103 76-77 0.22247881351257703 1103 78-79 0.21397923050985312 1103 80-81 0.54700950167258 1103 82-83 0.6443892501755926 1103 84-85 0.8042353176641797 1103 86-87 0.7392794205199245 1103 88-89 0.6815114070275641 1103 90-91 0.7262490620942685 1103 92-93 0.7542270948033227 1103 94-95 0.38553714623835234 1103 96-97 0.6234193677067381 1103 98-99 0.787522703116128 1103 100-101 0.9435812602994886 1104 1 0.10623433265213578 1104 2 0.1057298618570428 1104 3 0.06252308821282071 1104 4 0.060178918071137844 1104 5 0.03290037805837187 1104 6 0.029324247400779768 1104 7 0.06574446758469321 1104 8 0.10067920419920284 1104 9 0.06716140581080055 1104 10-11 0.1652609549283497 1104 12-13 0.1203672495371535 1104 14-15 0.13030117562608723 1104 16-17 0.06480788833572149 1104 18-19 0.0742497225081209 1104 20-21 0.06081741652115369 1104 22-23 0.13498153904774313 1104 24-25 0.13152845749361575 1104 26-27 0.20793508485203915 1104 28-29 0.08224996669678575 1104 30-31 0.08149779273895064 1104 32-33 0.08381291783501865 1104 34-35 0.19155863845249144 1104 36-37 0.1895696848097046 1104 38-39 0.25314815951128367 1104 40-41 0.2544295784378292 1104 42-43 0.38770296338547894 1104 44-45 0.3728319885032576 1104 46-47 0.29958823888369324 1104 48-49 0.288694239216845 1104 50-51 0.37658665293350424 1104 52-53 0.22937399081254028 1104 54-55 0.2959744365877057 1104 56-57 0.40795758656001624 1104 58-59 0.31018305338573526 1104 60-61 0.18746759381340894 1104 62-63 0.3711347622959238 1104 64-65 0.3237903637823649 1104 66-67 0.459159334889641 1104 68-69 0.4525522614320039 1104 70-71 0.3803101154917812 1104 72-73 0.29118349730382675 1104 74-75 0.2780688082099694 1104 76-77 0.25882256873954645 1104 78-79 0.5235787996903092 1104 80-81 0.453073333621699 1104 82-83 0.4540607268267678 1104 84-85 0.5208284354939501 1104 86-87 0.562342491187767 1104 88-89 0.5388114221906584 1104 90-91 0.35997741755478074 1104 92-93 0.6601201465094846 1104 94-95 0.40299151235054964 1104 96-97 0.626225713072067 1104 98-99 0.7081489702899013 1104 100-101 0.78646695399555 1105 1 0.12849125804500972 1105 2 0.12536294617167698 1105 3 0.08406250210440191 1105 4 0.04247326661290174 1105 5 0.05046534098694622 1105 6 0.006856289213416744 1105 7 0.010165463940857933 1105 8 -0.04247137473409879 1105 9 -0.014367597129584908 1105 10-11 0.03776549213343117 1105 12-13 -0.003164313934483687 1105 14-15 0.19841201004975773 1105 16-17 -0.025208150156665 1105 18-19 0.04061812949190369 1105 20-21 0.09577851614270116 1105 22-23 -0.01759517137305977 1105 24-25 0.07559773252561541 1105 26-27 -0.037200609234822934 1105 28-29 -0.03696597612739794 1105 30-31 0.07373882032796786 1105 32-33 0.12224198971279066 1105 34-35 0.09977695891282679 1105 36-37 0.2281675829230707 1105 38-39 0.16779242856402732 1105 40-41 0.026781468588232826 1105 42-43 0.07951067030313652 1105 44-45 0.17328289524082408 1105 46-47 0.29415062721217566 1105 48-49 0.3990749423781139 1105 50-51 0.27247362376877504 1105 52-53 0.3416891612566886 1105 54-55 0.2742732308870046 1105 56-57 0.15015640759013849 1105 58-59 0.21077253832503118 1105 60-61 0.22271710492077545 1105 62-63 0.2074928990170406 1105 64-65 0.34679997280893815 1105 66-67 0.29213742968557455 1105 68-69 0.31877505578758303 1105 70-71 0.3378573516656118 1105 72-73 0.2423294053779408 1105 74-75 0.24979041374747268 1105 76-77 0.11592171194640244 1105 78-79 0.21646629107313586 1105 80-81 0.36049676388942586 1105 82-83 0.4496255209291071 1105 84-85 0.660187458552798 1105 86-87 0.5524978113245176 1105 88-89 0.27655745125690956 1105 90-91 0.3339992805522911 1105 92-93 0.3070939362364058 1105 94-95 0.0018744454250132492 1105 96-97 0.16388451524992576 1105 98-99 0.4047506388558624 1105 100-101 0.2795696988287659 1106 1 -0.17996499229872143 1106 2 -0.027531515688302477 1106 3 -0.04641871661637964 1106 4 0.04568835763198109 1106 5 0.033013875332216 1106 6 -0.011767700227274247 1106 7 0.053879922501053557 1106 8 -0.1436922953742581 1106 9 -0.07441049434126512 1106 10-11 0.01172028507188827 1106 12-13 -0.11121689051699946 1106 14-15 -0.18478988182727107 1106 16-17 -0.03435460563705561 1106 18-19 -0.02431449988718981 1106 20-21 -0.08244729917930727 1106 22-23 -0.13211092784888478 1106 24-25 -0.03189278059102918 1106 26-27 -0.19778577119929253 1106 28-29 -0.20317342264337412 1106 30-31 -0.07523934609121596 1106 32-33 -0.11716404889469345 1106 34-35 -0.20922100621944395 1106 36-37 -0.3912329560418968 1106 38-39 -0.29972228297972237 1106 40-41 -0.23939298007260845 1106 42-43 -0.18880643908475747 1106 44-45 -0.23490882229753396 1106 46-47 -0.1996243274157692 1106 48-49 -0.3878656064862085 1106 50-51 -0.36598372650345823 1106 52-53 -0.47174206571355626 1106 54-55 -0.7288247177463347 1106 56-57 -0.395146897695021 1106 58-59 -0.29412984302017975 1106 60-61 -0.3238153704683242 1106 62-63 -0.29642504631651434 1106 64-65 -0.5769892808895705 1106 66-67 -0.5989712982471147 1106 68-69 -0.6531300203824664 1106 70-71 -0.6247230806062873 1106 72-73 -0.5572724205033772 1106 74-75 -0.5788181766941527 1106 76-77 -0.43696505041404876 1106 78-79 -0.5703131182652541 1106 80-81 -0.8496687753505228 1106 82-83 -0.8180860778169752 1106 84-85 -0.6388600033621756 1106 86-87 -0.5234269535657639 1106 88-89 -0.5342856439418995 1106 90-91 -0.8141874873077981 1106 92-93 -0.5290772126389172 1106 94-95 -0.6079413165504555 1106 96-97 -0.2439497255244234 1106 98-99 -0.20096678951129476 1106 100-101 0.015783744915765396 1107 1 0.11872496556031109 1107 2 0.1426872326723938 1107 3 0.05204112705985153 1107 4 0.07932804358557632 1107 5 0.021343099483708272 1107 6 0.030392855124283358 1107 7 0.03703437528043452 1107 8 -0.021474736053406218 1107 9 0.07250726956437603 1107 10-11 0.069784598139087 1107 12-13 0.08501420287507955 1107 14-15 0.14044888786619936 1107 16-17 0.1996104925461637 1107 18-19 0.09286030645669285 1107 20-21 0.014572057690024565 1107 22-23 0.07859252910961345 1107 24-25 0.1467167422193043 1107 26-27 0.13124358759348098 1107 28-29 0.18189975576506612 1107 30-31 0.16954916218835336 1107 32-33 0.08552283144942407 1107 34-35 0.12222962239795265 1107 36-37 0.1334130423144373 1107 38-39 0.17393628772443037 1107 40-41 0.23807733764584071 1107 42-43 0.2580754787665782 1107 44-45 0.3360654909445273 1107 46-47 0.29641914248228574 1107 48-49 0.39564206107223754 1107 50-51 0.49960773339267206 1107 52-53 0.3580234486336096 1107 54-55 0.5187297070556411 1107 56-57 0.38934400090436583 1107 58-59 0.45853448053854606 1107 60-61 0.6206372340535182 1107 62-63 0.714545944608318 1107 64-65 0.6666079143752555 1107 66-67 0.4535823396902501 1107 68-69 0.5719910693375851 1107 70-71 0.46675080423170456 1107 72-73 0.4648608568751378 1107 74-75 0.3501623964930012 1107 76-77 0.4059876153083799 1107 78-79 0.5863224235347424 1107 80-81 0.4876079736746384 1107 82-83 0.5379946370058022 1107 84-85 0.7042853567595238 1107 86-87 0.5047134230800943 1107 88-89 0.5644567715575803 1107 90-91 0.5932132366076388 1107 92-93 0.7397909263388591 1107 94-95 0.7441673057370473 1107 96-97 1.1615994185417406 1107 98-99 1.2466818841846958 1107 100-101 1.207680088618229 1108 1 0.07177233456621934 1108 2 0.04717002407124227 1108 3 0.017719650816651722 1108 4 0.03886771501088049 1108 5 0.06273123453912888 1108 6 0.09189116217869042 1108 7 0.04435942752812139 1108 8 0.08069777704366032 1108 9 0.12736198685117017 1108 10-11 0.1187198632199582 1108 12-13 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-0.6142290531311261 2316 72-73 -0.5120224707251246 2316 74-75 -0.4072917446016824 2316 76-77 -0.47908888951901574 2316 78-79 -0.4058699615579471 2316 80-81 -0.3383597647962304 2316 82-83 -0.4034197820186023 2316 84-85 -0.23171800716357538 2316 86-87 -0.16644396714881537 2316 88-89 -0.2911867302769515 2316 90-91 -0.37082556621002993 2316 92-93 -0.6372069547309209 2316 94-95 -0.18920659555158537 2316 96-97 -0.3371589725072539 2316 98-99 -0.1740191901108652 2316 100-101 0.10525757552167292 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 5.0 4 20.0 5 47.0 6 109.0 7 302.0 8 650.0 9 1092.0 10 1758.0 11 2905.0 12 4481.0 13 6633.0 14 9110.0 15 11996.0 16 16723.0 17 24748.0 18 24213.0 19 14736.0 20 11363.0 21 11338.0 22 11768.0 23 12827.0 24 14482.0 25 16943.0 26 19849.0 27 24137.0 28 28824.0 29 35010.0 30 42652.0 31 52227.0 32 65491.0 33 85793.0 34 119145.0 35 184995.0 36 253436.0 37 329153.0 38 195019.0 39 10321.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.22418238963112 19.950751199291616 15.579053508353685 20.246012902723585 2 15.729219001091192 21.676943981840232 40.46666999174003 22.127167025328543 3 18.99886448853632 27.36661693608962 31.022027219432267 22.612491355941792 4 12.414857593243099 18.19152662767955 37.33233027469373 32.06128550438362 5 12.326985963049388 39.222758822431146 33.46718944136208 14.983065773157382 6 29.219353598847903 37.6711970535862 17.466903930173498 15.642545417392403 7 26.607427823565473 35.88156447956096 20.211469411982215 17.29953828489136 8 26.46009028771531 34.91676392189012 19.192920508982855 19.430225281411715 9 27.410005832265504 16.592095972695997 20.424970853876513 35.57292734116199 10-11 24.854436892448113 27.927408800319164 26.067594556724305 21.15055975050842 12-13 26.354828547519194 25.971292413081766 27.44948622639123 20.224392813007814 14-15 23.017884770699336 26.33059336959589 27.689952709474646 22.961569150230126 16-17 20.22354206868702 30.053597308032177 28.077071140954292 21.64578948232651 18-19 20.040059502379123 27.249948914555944 32.70779004369022 20.002201539374713 20-21 21.578582792476332 25.900411359456644 32.95956830367134 19.561437544395684 22-23 21.03671218947348 26.23830508227189 32.78235046560734 19.942632262647297 24-25 20.38959423703651 26.532250633809817 33.13989856001937 19.938256569134303 26-27 20.256918168123644 26.68647218210125 33.16982834104781 19.88678130872729 28-29 19.87102141696426 26.84287698625072 33.33136694917728 19.95473464760774 30-31 20.161387432007707 26.645498642586773 33.21964794831126 19.97346597709426 32-33 20.023462893142575 26.811007855325535 32.9745177046625 20.19101154686938 34-35 20.37458881178772 26.950650115327345 32.838944389835625 19.835816683049313 36-37 20.5847552385033 26.630879185473443 32.75107975187373 20.033285824149523 38-39 20.26548185418814 26.88987636779024 32.74643186147207 20.09820991654955 40-41 20.31899493330576 26.849221748465695 32.69106546558236 20.140717852646176 42-43 20.318469458722955 26.85502438454752 32.41403827387145 20.412467882858078 44-45 20.30871553816311 27.167222791710216 32.11636570636902 20.407695963757654 46-47 20.470697632554565 27.444377681985817 31.439745935058237 20.645178750401385 48-49 20.554502087205286 27.475211396432776 31.278583522268388 20.69170299409355 50-51 20.691064426042875 27.86008548297638 30.718133629791083 20.730716461189658 52-53 20.816862332026194 28.07510046803997 30.422446214325333 20.685590985608503 54-55 20.500467066917068 28.007412254668235 30.48995197968259 21.002168698732106 56-57 20.659166431511448 28.23750960892876 30.26295624166821 20.84036771789158 58-59 20.74297088616217 27.917434975527637 30.40739425313081 20.932199885179383 60-61 20.715664499995135 28.087658973036618 30.533070527104478 20.663605999863773 62-63 20.602698771030177 28.03511394486664 30.523005478305716 20.83918180579747 64-65 20.44743551070848 27.98296422072804 30.45641195302085 21.113188315542626 66-67 20.469450904455623 28.131659352528487 30.298047076453017 21.100842666562876 68-69 20.43441731393008 28.095879418194247 30.30105008561422 21.168653182261448 70-71 20.53041894929405 28.148170897838842 30.107784205353756 21.213625947513357 72-73 20.54759947065749 28.117154735377397 29.917886230283454 21.417359563681654 74-75 20.343227286438516 28.460521898627018 29.670334682637762 21.525916132296704 76-77 20.166435160408295 28.168392219443604 30.041859656121982 21.623312964026116 78-79 20.18851137016026 28.244959569519988 30.002511700999328 21.564017359320417 80-81 20.165887816364858 28.53909617686267 29.82216183868676 21.472854168085707 82-83 20.21761797624344 28.40454940344042 29.712671657026817 21.66516096328932 84-85 20.14969784013397 28.1676610944047 29.66111204196697 22.021529023494363 86-87 20.096588125717822 28.04090369071609 29.73268588516157 22.12982229840452 88-89 20.115976227002026 28.070504058711826 29.644636555621606 22.16888315866454 90-91 20.055908255240375 28.152509789874237 29.745131821971764 22.04645013291362 92-93 20.03004927022115 28.016489696676306 29.6837346237714 22.26972640933115 94-95 19.834349366053967 27.90986338292676 29.742249608344927 22.513537642674347 96-97 19.856595729800326 27.790810997368187 29.711959934379472 22.64063333845202 98-99 19.82136514902354 27.849290642119705 29.526626463233075 22.80271774562368 100-101 19.824284317255202 27.646986202064827 29.76320072200761 22.765528758672364 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 5816.0 1 3624.5 2 2094.5 3 2715.0 4 2099.5 5 1935.5 6 3901.0 7 8492.5 8 13019.5 9 13802.0 10 10045.0 11 6275.5 12 5515.5 13 5511.0 14 5649.5 15 5610.5 16 5439.5 17 5441.0 18 5521.5 19 5642.5 20 5963.0 21 6573.0 22 7267.5 23 8048.0 24 9151.5 25 10727.0 26 12775.5 27 15011.0 28 17209.0 29 19518.0 30 22027.5 31 24652.0 32 27617.5 33 30522.5 34 33441.5 35 35836.0 36 38554.5 37 41787.5 38 43510.5 39 44441.5 40 46201.5 41 48819.0 42 50761.5 43 52824.0 44 54843.5 45 56579.5 46 56982.0 47 57369.0 48 61503.5 49 61118.5 50 56558.5 51 52917.0 52 49617.5 53 48047.0 54 45918.0 55 42028.0 56 37335.0 57 33021.5 58 29388.5 59 26096.5 60 22256.0 61 18622.0 62 15759.5 63 12278.5 64 9178.5 65 7284.0 66 5415.0 67 4182.5 68 3148.5 69 2048.0 70 1496.5 71 1168.5 72 956.5 73 822.5 74 652.5 75 514.0 76 334.0 77 175.5 78 95.5 79 48.0 80 24.0 81 13.5 82 7.5 83 3.5 84 3.5 85 3.0 86 1.5 87 0.5 88 1.5 89 1.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.013987681110062372 3 0.006750576535725753 4 0.0 5 6.081600482635814E-5 6 0.0 7 0.0 8 3.040800241317907E-4 9 1.8244801447907442E-4 10-11 0.0 12-13 0.0 14-15 0.0 16-17 3.040800241317907E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.004895688388521831 26-27 0.01930908153236871 28-29 0.0 30-31 0.0 32-33 3.3448802654496975E-4 34-35 0.017515009389991145 36-37 0.0036793682919946677 38-39 0.01669399332483531 40-41 0.014230945129367805 42-43 0.007754040615360663 44-45 0.0024022321906411468 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 5.777520458504023E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 3.040800241317907E-5 84-85 0.003557736282341951 86-87 1.5204001206589536E-4 88-89 9.122400723953721E-5 90-91 1.8244801447907442E-4 92-93 2.7367202171861164E-4 94-95 0.0 96-97 9.122400723953721E-5 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1644304.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.40755281915745 #Duplication Level Percentage of deduplicated Percentage of total 1 73.36067806696374 37.712929325766446 2 14.366499538492386 14.770931677028972 3 5.175441215127781 7.981703028873775 4 2.407834077859111 4.951234301492382 5 1.3284037633143315 3.4144993313874514 6 0.8012382153824888 2.471381752680166 7 0.5107590879338029 1.837981235357516 8 0.38039266888710316 1.5644044974267226 9 0.26130485810912907 1.2089738965632748 >10 1.2799266446276734 11.696462935737914 >50 0.08365335787701368 2.98919522632578 >100 0.03961194626597255 3.5503514275887507 >500 0.00283770610625812 1.022129215975921 >1k 9.459020354193734E-4 1.1604716336472576 >5k 1.1823775442742167E-4 0.3131547169783507 >10k+ 3.54713263282265E-4 3.354195797169318 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 23055 1.402112991271687 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 18755 1.140604170518347 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13373 0.8132924325428874 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5152 0.31332405686539716 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4039 0.24563584349366055 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3988 0.24253422724751628 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2420 0.1471747316797867 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2319 0.14103231519232454 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1999 0.12157119364788994 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1983 0.1205981375706682 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 9.122400723953722E-5 0.0 18-19 0.0 0.0 0.0 2.128560168922535E-4 0.0 20-21 0.0 0.0 0.0 3.344880265449698E-4 0.0 22-23 0.0 0.0 0.0 6.081600482635815E-4 0.0 24-25 0.0 0.0 0.0 0.0013987681110062373 0.0 26-27 0.0 0.0 0.0 0.0037097762944078468 0.0 28-29 0.0 0.0 0.0 0.00884872870223511 0.0 30-31 0.0 0.0 0.0 0.024812929969154122 0.0 32-33 0.0 0.0 0.0 0.055464196401638625 0.0 34-35 0.0 0.0 0.0 0.10007273594177232 0.0 36-37 0.0 0.0 0.0 0.16800421333281437 0.0 38-39 0.0 0.0 0.0 0.270844077494186 0.0 40-41 0.0 0.0 0.0 0.3867289746908114 0.0 42-43 0.0 0.0 0.0 0.49130209498973426 0.0 44-45 0.0 0.0 0.0 0.6086769843046055 0.0 46-47 0.0 0.0 0.0 0.7465468672459594 0.0 48-49 0.0 0.0 0.0 0.9008978874952563 0.0 50-51 0.0 0.0 0.0 1.0668039486615615 0.0 52-53 0.0 0.0 0.0 1.223131489067715 0.0 54-55 0.0 3.0408002413179073E-5 0.0 1.3937507906080628 0.0 56-57 0.0 6.0816004826358146E-5 0.0 1.5777496132102093 0.0 58-59 0.0 6.0816004826358146E-5 0.0 1.7758577489320708 0.0 60-61 0.0 6.0816004826358146E-5 0.0 2.00340083098989 0.0 62-63 0.0 6.0816004826358146E-5 0.0 2.22808556082087 0.0 64-65 0.0 6.0816004826358146E-5 0.0 2.4434654419134176 0.0 66-67 0.0 6.0816004826358146E-5 0.0 2.6807086767410406 0.0 68-69 0.0 6.0816004826358146E-5 0.0 2.94230872150162 0.0 70-71 0.0 6.0816004826358146E-5 0.0 3.22686680808415 0.0 72-73 0.0 6.0816004826358146E-5 0.0 3.5085057264350143 0.0 74-75 0.0 6.0816004826358146E-5 0.0 3.789658116747268 0.0 76-77 0.0 6.0816004826358146E-5 0.0 4.088416740456752 0.0 78-79 0.0 6.0816004826358146E-5 0.0 4.4164582704901285 0.0 80-81 0.0 6.0816004826358146E-5 0.0 4.7621972579279745 0.0 82-83 0.0 6.0816004826358146E-5 0.0 5.119248022263523 0.0 84-85 0.0 6.0816004826358146E-5 0.0 5.476420418608725 0.0 86-87 0.0 6.0816004826358146E-5 0.0 5.839491967422083 0.0 88-89 0.0 6.0816004826358146E-5 0.0 6.217645885432377 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 9120 0.0 46.463104 1 GTATCAA 14585 0.0 36.316723 1 TAGCGGA 55 0.002580956 34.5427 9 TATCAAC 18495 0.0 28.664745 2 ATCAACG 18840 0.0 28.061628 3 TCAACGC 19110 0.0 27.662632 4 CAACGCA 19390 0.0 27.26317 5 AACGCAG 19630 0.0 26.954042 6 GTACATG 12000 0.0 26.404795 1 TACATGG 12120 0.0 25.437843 2 TATAACG 95 0.0012260446 25.002571 2 ACGCAGA 22015 0.0 24.012394 7 ACATGGG 12275 0.0 23.992128 3 GAGTACT 13365 0.0 23.845835 12-13 CGCAGAG 22150 0.0 23.75883 8 TAACGCA 140 1.7811846E-5 23.748106 4 TAGCCCT 895 0.0 23.350094 4 AGTACTT 13590 0.0 22.821949 12-13 GCAGAGT 23030 0.0 22.562248 9 GTACTTT 14065 0.0 22.540861 14-15 >>END_MODULE