FastQCFastQC Report
Thu 26 May 2016
SRR937932_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937932_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1667900
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT236371.4171712932430003No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT181431.0877750464656155No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT124430.7460279393248996No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA55590.3332933629114455No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38880.23310750044966722No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37400.22423406679057495No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25140.1507284609389052No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25060.15024881587625158No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20830.12488758318844055No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20020.12003117692907248No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA90350.045.5797421
GTATCAA152300.039.3246271
TATCAAC190550.031.2691972
ATCAACG195300.030.4618153
TCAACGC198200.030.0880874
CAACGCA200700.029.7132975
AACGCAG204350.029.1799436
ACGCAGA228350.026.0435627
GTACATG132650.025.876881
CGCAGAG229300.025.8104958
TACATGG132400.025.4284482
GCAGAGT235900.024.9271589
GAGTACT133000.024.26775612-13
ACATGGG136400.023.7080173
GTACTTT141700.022.62546314-15
AGTACTT134900.022.55171212-13
CATGGGG42700.021.9401234
CATGGGA87500.021.7940034
ATGGGGA19450.021.516115
AGAGTAC217000.020.9638110-11
Produced by FastQC (version 0.11.5)