FastQCFastQC Report
Thu 26 May 2016
SRR937932_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937932_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1667900
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT228521.3701061214701122No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT186261.1167336171233289No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT132190.7925535104023024No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA53480.32064272438395586No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40470.24264044606990826No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39980.23970262006115473No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24780.14857005815696386No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24100.14449307512440793No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20260.12147011211703339No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20260.12147011211703339No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA96150.046.2908361
GTATCAA153100.035.400971
TATCAAC193350.027.9561122
ATCAACG195250.027.6815783
TCAACGC200000.027.0233294
GTATAGG4250.026.8242151
CAACGCA202500.026.6662565
AACGCAG206100.026.2227256
GTACATG125800.024.4680331
GAGTACT139550.024.14549412-13
TACATGG127150.023.5343842
ACGCAGA231350.023.3394977
CGCAGAG230800.023.3128138
GTACTTT148200.022.64005914-15
AGTACTT140250.022.61971712-13
CGTAGAC1050.00219822222.6162363
ACATGGG129750.022.2188193
GCAGAGT239450.022.1731579
TATACCG1100.002880482221.5875785
CATGGGA82750.020.3744984
Produced by FastQC (version 0.11.5)