FastQCFastQC Report
Thu 26 May 2016
SRR937931_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937931_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2268567
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88550.3903345151366479No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70350.31010765827061754No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43640.19236813371613004No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA42910.18915024330337168No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA41490.1828907852401979No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA36880.1625695868801759No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA35850.15802927574984563No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG28830.12708463095866246No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA27340.12051660806138853No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA27260.12016396253670268No Hit
CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG25170.11095109820428492No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC23890.10530876980931134No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA37100.055.145331
GTATCAA69750.044.4420931
ATCAACG87550.034.958143
TATCAAC88350.034.9105572
TCAACGC88100.034.793854
CAACGCA90350.033.927375
AACGCAG93000.032.951176
ACGCAGA107250.028.4806797
CGCAGAG107850.028.366288
TGGTATC16050.026.9454232
GTACATG74700.025.8559071
TACATGG73550.025.652312
GTGGTAT17850.025.1139491
GCAGAGT121100.024.8697939
ACATGGG73350.024.4912153
GTAAGGT13800.022.7291644
TAAGGTG14150.022.5028215
CCGTAGT1302.86444E-422.010671
TTAGCGC1302.932438E-421.9344394
GTATAGG7200.021.8578191
Produced by FastQC (version 0.11.5)