FastQCFastQC Report
Thu 26 May 2016
SRR937931_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937931_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2268567
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83980.37018963953896883No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72530.3197172488183069No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46200.20365279050607718No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA40750.1796288141368538No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA39220.17288446847723696No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA37360.164685460028291No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA35610.15697133917578807No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG28570.12593853300343344No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC26690.11765136317331602No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA26450.11659342659925848No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA25810.1137722624017717No Hit
CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG24670.10874706367499835No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA39100.054.7327841
GTATCAA71350.044.1907461
ATCAACG90600.034.5868873
TATCAAC91350.034.4115872
TCAACGC91600.034.20934
CAACGCA93050.033.6754765
AACGCAG96050.032.685176
TGGTATC16800.029.7229422
ACGCAGA110600.028.2134977
CGCAGAG110850.028.1498648
GTGGTAT18250.028.1431451
GCAGAGT124350.024.9410119
GTACATG70150.023.9308871
TACATGG68150.023.8655822
GAGTACT86150.022.16243212-13
CAGAGTA118750.021.49767510-11
ACATGGG71700.021.4208973
GTACTTT89400.021.30362514-15
GGACCGA4700.021.221116
AGAGTAC113900.020.99531710-11
Produced by FastQC (version 0.11.5)