##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937931_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2268567 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.29473451742884 33.0 31.0 34.0 31.0 34.0 2 32.54982726981394 34.0 31.0 34.0 31.0 34.0 3 32.6192270274583 34.0 31.0 34.0 31.0 34.0 4 36.13210542161638 37.0 35.0 37.0 35.0 37.0 5 36.04648264741575 37.0 35.0 37.0 35.0 37.0 6 36.09666586880617 37.0 35.0 37.0 35.0 37.0 7 36.06051838010515 37.0 35.0 37.0 35.0 37.0 8 36.05256357868205 37.0 35.0 37.0 35.0 37.0 9 37.70499703116549 39.0 37.0 39.0 35.0 39.0 10-11 37.66534292352838 39.0 37.0 39.0 35.0 39.0 12-13 37.570121138145794 39.0 37.0 39.0 35.0 39.0 14-15 38.74122188147848 40.0 38.0 41.0 34.5 41.0 16-17 38.61677768388591 40.0 38.0 41.0 34.0 41.0 18-19 38.886281956847654 40.0 38.0 41.0 35.0 41.0 20-21 38.84043980186611 40.0 38.0 41.0 35.0 41.0 22-23 38.71709122983804 40.0 38.0 41.0 34.0 41.0 24-25 38.578719297248 40.0 38.0 41.0 34.0 41.0 26-27 38.38182782346742 40.0 38.0 41.0 34.0 41.0 28-29 38.23741242819807 40.0 38.0 41.0 33.5 41.0 30-31 37.96009749767144 40.0 37.5 41.0 33.0 41.0 32-33 37.70391286658053 40.0 37.0 41.0 33.0 41.0 34-35 37.58995039599888 40.0 37.0 41.0 33.0 41.0 36-37 37.509788117344556 40.0 37.0 41.0 32.5 41.0 38-39 37.278668207727605 40.0 36.0 41.0 32.0 41.0 40-41 37.01207370115143 39.0 36.0 41.0 31.0 41.0 42-43 36.707573547530224 39.0 35.5 41.0 30.5 41.0 44-45 36.39946516898112 39.0 35.0 40.0 30.0 41.0 46-47 35.9865064598048 38.5 35.0 40.0 29.5 41.0 48-49 35.509193468828556 38.0 35.0 40.0 27.5 41.0 50-51 35.044690106133075 38.0 34.0 40.0 26.5 41.0 52-53 34.40319527701849 38.0 33.0 40.0 24.0 41.0 54-55 33.91633837572353 37.0 33.0 40.0 23.5 41.0 56-57 33.44566503876676 37.0 32.5 39.5 22.5 41.0 58-59 32.83429936166752 36.0 31.5 39.0 20.0 40.0 60-61 32.23869826194245 35.5 31.0 39.0 17.5 40.0 62-63 31.571982886112686 35.0 30.5 38.5 13.0 40.0 64-65 30.89475162073679 35.0 29.0 38.0 8.0 40.0 66-67 30.220894291418325 34.0 28.5 37.5 4.5 39.5 68-69 29.47598924783795 34.0 28.0 37.0 2.0 39.0 70-71 28.755181134169717 33.5 26.0 36.0 2.0 39.0 72-73 27.9862241229816 33.0 26.0 35.5 2.0 38.5 74-75 27.261216001114363 32.0 25.0 35.0 2.0 37.5 76-77 26.08665249913271 30.5 23.0 34.0 2.0 36.0 78-79 26.70698220506602 32.0 25.0 35.0 2.0 36.0 80-81 26.372262534013764 32.0 23.5 35.0 2.0 36.0 82-83 25.725324400822196 32.0 21.0 35.0 2.0 36.0 84-85 24.977077600088514 31.0 19.0 34.0 2.0 35.0 86-87 24.140124801251186 31.0 11.5 34.0 2.0 35.0 88-89 23.196440528315893 30.0 4.5 34.0 2.0 35.0 90-91 22.408656874582057 29.5 2.0 34.0 2.0 35.0 92-93 21.336440140405816 29.0 2.0 34.0 2.0 35.0 94-95 20.298860690471123 28.0 2.0 34.0 2.0 35.0 96-97 19.1155317431665 25.5 2.0 33.0 2.0 35.0 98-99 17.596410421204222 22.5 2.0 32.5 2.0 34.0 100-101 14.175487433256325 2.0 2.0 28.5 2.0 32.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2187808508991651 1101 2 0.22747628622362726 1101 3 0.18988303670793272 1101 4 0.08415058506930961 1101 5 0.025235783604877327 1101 6 0.09069893253408168 1101 7 0.13804794052298774 1101 8 0.16392488271915795 1101 9 -0.00903453007931887 1101 10-11 0.165676018022765 1101 12-13 0.22632001845573058 1101 14-15 0.4765635668895669 1101 16-17 0.47288009154640775 1101 18-19 0.339958882878328 1101 20-21 0.397230458320756 1101 22-23 0.33783812855234885 1101 24-25 0.49772711457070073 1101 26-27 0.509818104964765 1101 28-29 0.4543805508233447 1101 30-31 0.6094087614704193 1101 32-33 0.574903989851606 1101 34-35 0.5374193639887537 1101 36-37 0.6554101550682461 1101 38-39 0.647703822134396 1101 40-41 0.7947479040645078 1101 42-43 1.104235278547094 1101 44-45 1.0206771831188988 1101 46-47 1.1200362152480068 1101 48-49 1.103182370209808 1101 50-51 1.2389496051299034 1101 52-53 1.4158793161850127 1101 54-55 1.6523818527984773 1101 56-57 1.5436567466474571 1101 58-59 1.8143545649328416 1101 60-61 1.8525642342996704 1101 62-63 1.8215255782790614 1101 64-65 1.948741539118842 1101 66-67 2.0282004699224636 1101 68-69 2.5054570476762557 1101 70-71 2.7024978796274404 1101 72-73 2.765853460002539 1101 74-75 2.6952161671488106 1101 76-77 2.7005252842670906 1101 78-79 2.918689039535817 1101 80-81 3.1933971024888663 1101 82-83 3.3778444381704986 1101 84-85 3.5438793818773355 1101 86-87 3.7475906946942246 1101 88-89 3.9256762879917346 1101 90-91 4.1714214304573005 1101 92-93 4.327261704043178 1101 94-95 4.57871611555683 1101 96-97 4.847373676819156 1101 98-99 4.968868306739299 1101 100-101 4.175256739151244 1102 1 0.21658740659556486 1102 2 0.19027316612857703 1102 3 0.1710049781710481 1102 4 0.17041811374898685 1102 5 0.01177179728476574 1102 6 0.10980481258075514 1102 7 0.1252694055477548 1102 8 0.1757543821608607 1102 9 0.20969001384717956 1102 10-11 0.246315229548415 1102 12-13 0.3047353211735597 1102 14-15 0.397134988270615 1102 16-17 0.42479816322276065 1102 18-19 0.36055421227246853 1102 20-21 0.3011871351466695 1102 22-23 0.3744220244855967 1102 24-25 0.4122264966710816 1102 26-27 0.4105054768979457 1102 28-29 0.4007309032181894 1102 30-31 0.4853400812324793 1102 32-33 0.40727106858380324 1102 34-35 0.48424986346092425 1102 36-37 0.46461758787745566 1102 38-39 0.6709267150172238 1102 40-41 0.7195207779401329 1102 42-43 0.8980789982895416 1102 44-45 0.7270576423911734 1102 46-47 0.7314796764395339 1102 48-49 0.7557009237551426 1102 50-51 0.9268314876243338 1102 52-53 1.0904283421818235 1102 54-55 1.281575523599038 1102 56-57 1.3713303049147996 1102 58-59 1.5020463754118438 1102 60-61 1.4720029721685037 1102 62-63 1.6789235519921668 1102 64-65 1.6782425702760833 1102 66-67 1.799171307444663 1102 68-69 2.1145073896072013 1102 70-71 2.217303628676749 1102 72-73 2.4426710981557527 1102 74-75 2.5059604017413903 1102 76-77 2.427097749388544 1102 78-79 2.5772273128871923 1102 80-81 2.8033160139259046 1102 82-83 2.8596872625214402 1102 84-85 3.128094861289295 1102 86-87 3.2967266286365806 1102 88-89 3.4211185332521836 1102 90-91 3.641331440960929 1102 92-93 3.8728316353126075 1102 94-95 4.144430037967716 1102 96-97 4.050024552276291 1102 98-99 4.181702273866776 1102 100-101 3.6538067479594574 1103 1 0.20171841703113103 1103 2 0.17915218767866747 1103 3 0.1954756915366147 1103 4 0.09014846003234567 1103 5 -0.044419111626211816 1103 6 0.06538931041783513 1103 7 0.1457632084860947 1103 8 0.17855753821770293 1103 9 0.19037211658855568 1103 10-11 0.1938686745260938 1103 12-13 0.24911128227100932 1103 14-15 0.3671857505655609 1103 16-17 0.2928680429129358 1103 18-19 0.2027533064851994 1103 20-21 0.29116590602620107 1103 22-23 0.3473430636884842 1103 24-25 0.4254219827077961 1103 26-27 0.5042477052929968 1103 28-29 0.4915030339523909 1103 30-31 0.5477420353097671 1103 32-33 0.5597031668074237 1103 34-35 0.5262253505819032 1103 36-37 0.5769815498319772 1103 38-39 0.6767677349787888 1103 40-41 0.7415336203963108 1103 42-43 0.7949680328937845 1103 44-45 0.8834122229159718 1103 46-47 0.8088885190093009 1103 48-49 0.9338205159013171 1103 50-51 0.9576694312135672 1103 52-53 1.1309415843512767 1103 54-55 1.3872813983085095 1103 56-57 1.474265938600169 1103 58-59 1.6348750976287647 1103 60-61 1.6143344579573906 1103 62-63 1.8258136622510683 1103 64-65 1.8398261232580921 1103 66-67 1.9791573755654461 1103 68-69 2.055736845678755 1103 70-71 2.1272151840475786 1103 72-73 2.251198328998992 1103 74-75 2.358092736522984 1103 76-77 2.344576965589109 1103 78-79 2.491877987580825 1103 80-81 2.7239681962571147 1103 82-83 2.9661516889006236 1103 84-85 3.0619199409092523 1103 86-87 3.2628247902997316 1103 88-89 3.5958278698486232 1103 90-91 3.775595947024609 1103 92-93 3.891674738602134 1103 94-95 3.8155378676149923 1103 96-97 3.8914917710549837 1103 98-99 4.262985409408731 1103 100-101 3.946066853304771 1104 1 0.21256834776248468 1104 2 0.20977072728585 1104 3 0.25398232875557625 1104 4 0.1592166348357651 1104 5 0.0923375033524394 1104 6 0.16082300902130697 1104 7 0.17683156622391039 1104 8 0.17780470889507427 1104 9 0.18223153610180276 1104 10-11 0.30679537004181867 1104 12-13 0.4176370858353877 1104 14-15 0.4907605433756572 1104 16-17 0.39918550258069985 1104 18-19 0.27479113715059356 1104 20-21 0.37591924322148174 1104 22-23 0.46856737926117376 1104 24-25 0.4460740435806727 1104 26-27 0.49429574018919453 1104 28-29 0.5445013829486101 1104 30-31 0.4450766853809185 1104 32-33 0.4949401107004405 1104 34-35 0.5073347641435149 1104 36-37 0.5775956121407759 1104 38-39 0.5928307111877089 1104 40-41 0.6709900671839151 1104 42-43 0.6696681019327215 1104 44-45 0.7835985403422256 1104 46-47 0.8392014370592875 1104 48-49 1.0519306657947496 1104 50-51 1.064409039140891 1104 52-53 1.2133770216668012 1104 54-55 1.2523239514377877 1104 56-57 1.2335747097195906 1104 58-59 1.4013123584409968 1104 60-61 1.5683118400826928 1104 62-63 1.7765210921022039 1104 64-65 1.8360078898780756 1104 66-67 2.1576825484655977 1104 68-69 2.3942230051048234 1104 70-71 2.362390815682865 1104 72-73 2.502339267419014 1104 74-75 2.5720490227209183 1104 76-77 2.5948320504760964 1104 78-79 2.528224399331055 1104 80-81 2.8592400355120766 1104 82-83 3.066510728971192 1104 84-85 3.3000353658286734 1104 86-87 3.3129601951631997 1104 88-89 3.432945780253373 1104 90-91 3.6398986182426434 1104 92-93 3.7801797761893 1104 94-95 4.019857625239453 1104 96-97 4.39839169717121 1104 98-99 4.4057993246182185 1104 100-101 3.704203883694852 1105 1 0.25865763689155585 1105 2 0.2494891563389814 1105 3 0.25520516597770637 1105 4 0.11221424674590708 1105 5 0.06670666222635191 1105 6 0.07382979515863042 1105 7 0.17022820591753174 1105 8 0.22937848782174797 1105 9 0.21310964996874304 1105 10-11 0.19425749540052806 1105 12-13 0.27425146189655436 1105 14-15 0.3740611504508564 1105 16-17 0.42365089453816296 1105 18-19 0.35774399620573405 1105 20-21 0.3542848314578748 1105 22-23 0.34731133558177874 1105 24-25 0.3571288497613594 1105 26-27 0.4659575390255952 1105 28-29 0.4199949546022168 1105 30-31 0.4806208214126144 1105 32-33 0.5942899354998303 1105 34-35 0.43562917743616225 1105 36-37 0.4939916345197517 1105 38-39 0.6036114107991821 1105 40-41 0.652089056269773 1105 42-43 0.6702801101470754 1105 44-45 0.7975365655682012 1105 46-47 0.6823858293954714 1105 48-49 0.9428584792882901 1105 50-51 0.9107729654821455 1105 52-53 1.0509531075146157 1105 54-55 1.1580196567870829 1105 56-57 1.2326777265820468 1105 58-59 1.4804119634657553 1105 60-61 1.414876624611189 1105 62-63 1.753656571342571 1105 64-65 1.9130796526563074 1105 66-67 2.017169668286936 1105 68-69 2.239078922766332 1105 70-71 2.404219238374182 1105 72-73 2.416280476502756 1105 74-75 2.450500241008367 1105 76-77 2.45532503350908 1105 78-79 2.6273195155094378 1105 80-81 2.8779093323335054 1105 82-83 3.1278165980592156 1105 84-85 3.326910662964167 1105 86-87 3.534759420699622 1105 88-89 3.507649499687112 1105 90-91 3.563949165325706 1105 92-93 3.9654739105995915 1105 94-95 4.190095846587141 1105 96-97 4.077792746704372 1105 98-99 4.117196375854942 1105 100-101 3.632320236794115 1106 1 0.19187993107761514 1106 2 0.13397486148567594 1106 3 0.16060477225823178 1106 4 0.0995023503226875 1106 5 0.043178039099501575 1106 6 0.0051491700044863364 1106 7 0.10804215031362219 1106 8 0.10598244132944501 1106 9 0.08244232820081265 1106 10-11 0.16935748003690065 1106 12-13 0.15135265043648616 1106 14-15 0.1264603845558554 1106 16-17 0.09717662124430859 1106 18-19 0.06020632190949726 1106 20-21 0.04011385584070126 1106 22-23 0.02187531770883311 1106 24-25 0.07767055599146033 1106 26-27 -0.036497443912651306 1106 28-29 0.22175123174648093 1106 30-31 0.20544797096847844 1106 32-33 0.38911262708643335 1106 34-35 0.25297052284391697 1106 36-37 0.3571318815350324 1106 38-39 0.3866283303521172 1106 40-41 0.253656746396544 1106 42-43 0.2257327141455363 1106 44-45 0.4672929736810545 1106 46-47 0.6254916022234767 1106 48-49 0.6803523837273389 1106 50-51 0.9353480780017875 1106 52-53 0.9996881078817523 1106 54-55 1.0030313131851827 1106 56-57 1.1715973480983237 1106 58-59 1.1718048279638253 1106 60-61 1.0483779826353299 1106 62-63 0.9185858376491787 1106 64-65 1.1996373738648671 1106 66-67 1.4143716290184472 1106 68-69 1.3275568255949999 1106 70-71 1.3375442150332937 1106 72-73 1.575197877752732 1106 74-75 1.6278454651012382 1106 76-77 1.818729736327601 1106 78-79 1.9203156215188208 1106 80-81 1.8716121843682565 1106 82-83 2.348333699243881 1106 84-85 2.5195174086601746 1106 86-87 2.5687128334678597 1106 88-89 2.7396110468446153 1106 90-91 2.7896046524542655 1106 92-93 2.6863424570788155 1106 94-95 2.92012056981428 1106 96-97 3.7386592744074214 1106 98-99 3.9544892163873193 1106 100-101 3.6739798722945807 1107 1 0.2720178724573046 1107 2 0.2757045758804182 1107 3 0.25204126970584895 1107 4 0.15647598377573502 1107 5 0.08425964625902083 1107 6 0.16409907593274653 1107 7 0.20037291120547707 1107 8 0.16062760774103424 1107 9 0.19722317994148852 1107 10-11 0.2518354273412129 1107 12-13 0.2698721997913722 1107 14-15 0.45156782051057576 1107 16-17 0.46037689467604537 1107 18-19 0.3655123380643346 1107 20-21 0.37850025842079305 1107 22-23 0.3907145239689527 1107 24-25 0.4863524315692729 1107 26-27 0.4280673422410075 1107 28-29 0.43045710024339456 1107 30-31 0.5405248030198351 1107 32-33 0.44493664956895174 1107 34-35 0.45637984502803874 1107 36-37 0.47251424729654445 1107 38-39 0.5222801369930608 1107 40-41 0.5727779888239013 1107 42-43 0.5196012102689593 1107 44-45 0.7106717934244031 1107 46-47 0.7803840986270245 1107 48-49 0.8096288579539461 1107 50-51 0.8332146379802055 1107 52-53 1.024404892957918 1107 54-55 1.1752106481197657 1107 56-57 1.1906510283753704 1107 58-59 1.284951236695349 1107 60-61 1.468527419129991 1107 62-63 1.51301667527461 1107 64-65 1.8128246660530607 1107 66-67 2.021840907239419 1107 68-69 2.237456351764692 1107 70-71 2.34236428505044 1107 72-73 2.4963924784307814 1107 74-75 2.4322058896764354 1107 76-77 2.385863057976156 1107 78-79 2.3623231842619994 1107 80-81 2.6132270470863865 1107 82-83 2.9338328444712687 1107 84-85 3.139051782893606 1107 86-87 3.3478738293371393 1107 88-89 3.547215389682343 1107 90-91 3.4473862464634344 1107 92-93 3.666372828215053 1107 94-95 3.7203150596792653 1107 96-97 3.7642558993238886 1107 98-99 3.6178656766280106 1107 100-101 3.2322983824735694 1108 1 0.21868984828689264 1108 2 0.29744513134416195 1108 3 0.189629400714324 1108 4 0.12547996204154543 1108 5 0.027306994049993705 1108 6 0.14349644068433065 1108 7 0.18842644606638004 1108 8 0.19291259353718004 1108 9 0.21852290799891705 1108 10-11 0.25084218676548886 1108 12-13 0.2981125198855352 1108 14-15 0.36150135061104294 1108 16-17 0.39102549434897327 1108 18-19 0.3231877096226512 1108 20-21 0.38774681480141027 1108 22-23 0.42662063748986156 1108 24-25 0.42585014289436174 1108 26-27 0.40597961928427395 1108 28-29 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0.5524364153307886 2311 60-61 0.6941461246127787 2311 62-63 0.7464948898753967 2311 64-65 0.8667624292155942 2311 66-67 1.2385366482241125 2311 68-69 1.8053050229357872 2311 70-71 2.0242592840861384 2311 72-73 2.1413886993142377 2311 74-75 2.4215415533136344 2311 76-77 2.520476333226231 2311 78-79 2.3167488729564987 2311 80-81 2.3440126638523715 2311 82-83 2.237634588494508 2311 84-85 2.7052127452916714 2311 86-87 2.8810765734006907 2311 88-89 2.922243583235577 2311 90-91 3.2262619838088433 2311 92-93 3.4384222752582083 2311 94-95 3.5116459007206515 2311 96-97 3.813661265051934 2311 98-99 3.770267767363851 2311 100-101 3.667549640151204 2312 1 -0.22643246341372247 2312 2 -0.22909470759473294 2312 3 -0.20944516110471767 2312 4 -0.14226006638099875 2312 5 -0.07682356018970182 2312 6 -0.049558604318036714 2312 7 -0.1819239092154774 2312 8 -0.21754292517432816 2312 9 -0.14591555718345717 2312 10-11 -0.16900120499197868 2312 12-13 -0.1392336667558638 2312 14-15 -0.14207875360496303 2312 16-17 -0.10950174392673517 2312 18-19 -0.16903445996522493 2312 20-21 -0.1960659753454408 2312 22-23 -0.28107732556082965 2312 24-25 -0.1416087865229798 2312 26-27 -0.1325623039748436 2312 28-29 -0.2991045656768918 2312 30-31 -0.3330056940692856 2312 32-33 -0.20241193977274463 2312 34-35 -0.1473748347749151 2312 36-37 -0.18282493765643437 2312 38-39 -0.08804301571630191 2312 40-41 -0.03295071695308138 2312 42-43 0.24871316727747228 2312 44-45 0.34695383552023173 2312 46-47 0.44035024282764823 2312 48-49 0.42422088661493973 2312 50-51 0.4433020540124559 2312 52-53 0.43312249259965085 2312 54-55 0.44289492935576646 2312 56-57 0.5693046781738502 2312 58-59 0.698142011437838 2312 60-61 0.6734395552697165 2312 62-63 0.7153380366718345 2312 64-65 0.9959694040736622 2312 66-67 1.1548550586052961 2312 68-69 1.5597989402107295 2312 70-71 1.6747877512394247 2312 72-73 1.4917121380814748 2312 74-75 1.7139550164117665 2312 76-77 2.0417348895928136 2312 78-79 1.9566663101016744 2312 80-81 1.9395683816139275 2312 82-83 2.1124452940906124 2312 84-85 2.252525316256797 2312 86-87 2.534884845906774 2312 88-89 2.5552056270311994 2312 90-91 2.320832462316549 2312 92-93 2.5914858600108417 2312 94-95 2.5165041326752338 2312 96-97 2.7883890833812117 2312 98-99 2.615553898750715 2312 100-101 2.379733581757044 2313 1 -0.25164696301970935 2313 2 -0.34357666188945046 2313 3 -0.3539955157146437 2313 4 -0.19494974329195713 2313 5 -0.209251928984024 2313 6 -0.2603570282739085 2313 7 -0.3122167382146941 2313 8 -0.2979555335273645 2313 9 -0.35652359500851816 2313 10-11 -0.289046437458488 2313 12-13 -0.28116731529802763 2313 14-15 -0.36311862752143753 2313 16-17 -0.32114319388733037 2313 18-19 -0.3777428918013115 2313 20-21 -0.36157015186238795 2313 22-23 -0.3522896187050293 2313 24-25 -0.38399791181848997 2313 26-27 -0.4047467011379666 2313 28-29 -0.3772120518865023 2313 30-31 -0.4139160171583285 2313 32-33 -0.3017309311045011 2313 34-35 -0.23660210033835227 2313 36-37 -0.3869516516044271 2313 38-39 -0.2232527871898995 2313 40-41 -0.12174929851336458 2313 42-43 -0.07760755770282657 2313 44-45 -0.029772248010104363 2313 46-47 -0.037576628724750094 2313 48-49 0.08536194256450358 2313 50-51 0.0732598160297897 2313 52-53 0.19978506155158016 2313 54-55 0.3843630144621528 2313 56-57 0.539560942949258 2313 58-59 0.5744895291683321 2313 60-61 0.5760334086976044 2313 62-63 0.879841740375543 2313 64-65 1.0509539588411947 2313 66-67 1.0266550586052965 2313 68-69 1.3243671041193181 2313 70-71 1.6259155684183035 2313 72-73 1.7598379064029892 2313 74-75 1.8688648666875736 2313 76-77 1.8901924152035328 2313 78-79 1.8937564598258696 2313 80-81 1.9645340238519076 2313 82-83 2.1047709048077152 2313 84-85 2.1403777985262984 2313 86-87 2.3026856339288386 2313 88-89 2.8039726877088995 2313 90-91 3.3258504292196207 2313 92-93 3.293118484124321 2313 94-95 3.530193179168535 2313 96-97 3.619599327668052 2313 98-99 3.3411840011935823 2313 100-101 3.159890870961142 2314 1 -0.18969772492816617 2314 2 -0.23474841563532323 2314 3 -0.2484347005270422 2314 4 -0.14598902812963388 2314 5 -0.10318452818345492 2314 6 -0.17534845599641358 2314 7 -0.17332000601485475 2314 8 -0.17123254266066823 2314 9 -0.2849150887993872 2314 10-11 -0.23078309414108134 2314 12-13 -0.1973103256161366 2314 14-15 -0.2918995186322846 2314 16-17 -0.15362898826709426 2314 18-19 -0.24268707510963594 2314 20-21 -0.27700680282396917 2314 22-23 -0.19525499925325107 2314 24-25 -0.1447455546728591 2314 26-27 -0.19364583246820644 2314 28-29 -0.10906678269484615 2314 30-31 -0.16779476510753 2314 32-33 -0.005787583800845653 2314 34-35 0.09665279988550424 2314 36-37 0.056579277800246075 2314 38-39 0.10718820988869737 2314 40-41 0.05495357656760547 2314 42-43 0.07533377617677672 2314 44-45 0.17481129063342138 2314 46-47 0.4008492279954936 2314 48-49 0.4198196375907344 2314 50-51 0.4664196184152658 2314 52-53 0.47122959642479145 2314 54-55 0.6740635476227439 2314 56-57 0.8573880505391926 2314 58-59 0.9789109419608693 2314 60-61 1.2219785092119437 2314 62-63 1.3437805034946315 2314 64-65 1.4115077335037967 2314 66-67 1.4614716081759447 2314 68-69 1.847787230608855 2314 70-71 2.0772146052597193 2314 72-73 2.249376775083814 2314 74-75 2.440371409854393 2314 76-77 2.5070568255803245 2314 78-79 2.4795489018268917 2314 80-81 2.586796016274345 2314 82-83 2.884481828048461 2314 84-85 3.0841218814402467 2314 86-87 3.2823483900129418 2314 88-89 3.310978148498805 2314 90-91 3.547368137570256 2314 92-93 3.8785570543902352 2314 94-95 4.21705729426774 2314 96-97 4.341944274638042 2314 98-99 4.412466779312776 2314 100-101 4.529538733981866 2315 1 -0.24208710995860372 2315 2 -0.30651615318647174 2315 3 -0.33713253203402616 2315 4 -0.2502308368337438 2315 5 -0.21128551412139274 2315 6 -0.21633795300746783 2315 7 -0.28992835719006393 2315 8 -0.2621017814094344 2315 9 -0.24051253891499158 2315 10-11 -0.2192723725853014 2315 12-13 -0.2613240559536436 2315 14-15 -0.34983538585277785 2315 16-17 -0.32169634281473947 2315 18-19 -0.3771845791074 2315 20-21 -0.34911029623503254 2315 22-23 -0.24381155907948937 2315 24-25 -0.24611220193202854 2315 26-27 -0.25811591795419275 2315 28-29 -0.28050440682065414 2315 30-31 -0.19560871233775146 2315 32-33 -0.153765236039618 2315 34-35 -0.17080883000922853 2315 36-37 -0.035854961484865555 2315 38-39 0.07563196442666964 2315 40-41 -0.04484062958215418 2315 42-43 0.0669728972218735 2315 44-45 0.018598474122299535 2315 46-47 0.20721187904687355 2315 48-49 0.44087537430358026 2315 50-51 0.27448585067647713 2315 52-53 0.35032090403730365 2315 54-55 0.7268925465122464 2315 56-57 0.792614606688538 2315 58-59 1.1180665547261697 2315 60-61 1.0586878475651886 2315 62-63 0.9076709993406133 2315 64-65 1.26996416181791 2315 66-67 1.5814604597172917 2315 68-69 1.6569053738882502 2315 70-71 1.858531357275961 2315 72-73 2.058223337446048 2315 74-75 2.301648900446718 2315 76-77 2.514759988877959 2315 78-79 2.357517302953145 2315 80-81 2.5661572616774677 2315 82-83 2.893154746036604 2315 84-85 3.1438619326189894 2315 86-87 3.205751565525521 2315 88-89 3.6699549518922012 2315 90-91 3.739264392419802 2315 92-93 4.080882047461202 2315 94-95 4.282163703763398 2315 96-97 4.4706558029999535 2315 98-99 4.812051595335305 2315 100-101 4.916497362535438 2316 1 -0.36234108961180667 2316 2 -0.35422314209952077 2316 3 -0.3199365349066383 2316 4 -0.2696447309177401 2316 5 -0.20418202664337315 2316 6 -0.27687937109119787 2316 7 -0.27279675266596115 2316 8 -0.2596266312446147 2316 9 -0.3744561322633331 2316 10-11 -0.3636970516373381 2316 12-13 -0.43905826739484866 2316 14-15 -0.47493562261454514 2316 16-17 -0.3431550985593219 2316 18-19 -0.33613350149877164 2316 20-21 -0.4207611830131768 2316 22-23 -0.3357156642189665 2316 24-25 -0.28210750856770517 2316 26-27 -0.36380511547644545 2316 28-29 -0.34695376695485436 2316 30-31 -0.26334243528334156 2316 32-33 -0.35621098130629036 2316 34-35 -0.3348310648068633 2316 36-37 -0.3451367587426901 2316 38-39 -0.3132264022977651 2316 40-41 -0.3033126977837526 2316 42-43 -0.21011910109313447 2316 44-45 -0.17843210697177625 2316 46-47 -0.36327883065477806 2316 48-49 -0.28955387376844044 2316 50-51 -0.11521136451789715 2316 52-53 0.0821068376475722 2316 54-55 0.35043007312573593 2316 56-57 0.5558634641163422 2316 58-59 0.6751228421087632 2316 60-61 0.8822437086243511 2316 62-63 0.9862418705376506 2316 64-65 1.3697419280353245 2316 66-67 1.3387556975829327 2316 68-69 1.7780066079423555 2316 70-71 2.000969540376804 2316 72-73 1.9520178888801958 2316 74-75 2.416259169766402 2316 76-77 2.574267477452242 2316 78-79 2.4729596008365 2316 80-81 2.503355282572393 2316 82-83 2.679433792493171 2316 84-85 2.9592732395794776 2316 86-87 3.224735964117638 2316 88-89 3.4968934864561234 2316 90-91 3.6413295869171876 2316 92-93 3.920075636368672 2316 94-95 4.455506688585778 2316 96-97 4.7110858884930344 2316 98-99 4.919137604821 2316 100-101 4.840513773450333 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 10.0 4 22.0 5 54.0 6 103.0 7 352.0 8 780.0 9 1475.0 10 2509.0 11 3918.0 12 5984.0 13 8823.0 14 12120.0 15 16605.0 16 21244.0 17 28445.0 18 29646.0 19 27641.0 20 28269.0 21 29837.0 22 33393.0 23 36769.0 24 40793.0 25 44974.0 26 50719.0 27 58757.0 28 68806.0 29 81041.0 30 95662.0 31 110498.0 32 129985.0 33 156628.0 34 192797.0 35 245060.0 36 300540.0 37 293237.0 38 108352.0 39 2718.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.761590466580884 18.3239463502731 14.135928099103973 23.77853508404204 2 16.343133952771513 19.45481445951403 39.06102036245775 25.14103122525671 3 19.909808640914083 22.552644687482093 29.455148974436966 28.08239769716686 4 13.455829908936465 15.914518943137526 35.218948339141804 35.41070280878421 5 13.99747654060991 36.818808289903906 34.06193552600977 15.121779643476408 6 34.29627601917863 35.06208985672453 15.99229822174086 14.64933590235598 7 29.930568504258414 32.142449396469225 19.3372291847673 18.58975291450506 8 27.34543264928646 34.746786284503195 18.61023610593161 19.29754496027873 9 26.346720198257312 16.361606247468117 19.46114000600379 37.83053354827078 10-11 25.842789743481237 25.860091414536136 26.291619335025153 22.005499506957477 12-13 26.485684575328833 23.000863540728574 27.092785886420813 23.420665997521784 14-15 22.83305841663853 24.626151498347415 25.228778884987257 27.312011200026802 16-17 22.70872877403611 27.895485518164335 26.298904241710403 23.09688146608915 18-19 22.841798368749963 26.125016364956384 28.59271954498148 22.440465721312176 20-21 22.9288136519662 25.322527392843146 29.191798170386857 22.5568607848038 22-23 22.747030938541684 25.35522502801334 29.040092358066477 22.8576516753785 24-25 22.739421023770188 25.561127871650513 29.02097193104336 22.67847917353594 26-27 22.150097038373413 25.99391870818285 28.84727669877768 23.008707554666053 28-29 22.392990817551343 26.433603239401787 28.660934413662897 22.51247152938397 30-31 22.503677431612115 25.892071955556084 28.614826011310225 22.989424601521577 32-33 22.082662755827798 25.908381811072807 28.676538978130246 23.332416454969152 34-35 22.97705801184131 26.092720195033355 28.45606679804085 22.47415499508449 36-37 23.121173630474615 25.558765387854493 28.42012994641292 22.899931035257975 38-39 22.527413790025214 25.891833975885074 28.461916052493585 23.118836181596123 40-41 23.010081982371794 25.77369444400972 28.533780118486824 22.68244345513166 42-43 22.643894497609924 25.79090093352639 28.3964342920018 23.16877027686189 44-45 22.624101078980736 26.112532888160743 28.613124142185736 22.650241890672778 46-47 23.235989944312863 26.349431160728336 27.79056117804764 22.624017716911162 48-49 22.846404800916172 26.508165727527555 27.61990278444498 23.02552668711129 50-51 23.039764750170484 26.742366436609544 27.606590415888093 22.611278397331883 52-53 23.458531310734926 26.2988706086265 27.61595756263756 22.62664051800101 54-55 22.792361874258066 26.63033536148591 27.80103034206175 22.776272422194275 56-57 22.89278777871131 26.769091135104734 27.47277354945812 22.865347536725842 58-59 22.843495475337512 26.288798170827665 27.495242591468532 23.372463762366287 60-61 22.46457785906257 26.640385758939455 27.87742217884682 23.01761420315115 62-63 22.68085976742146 26.616648307059037 27.899242120686758 22.803249804832742 64-65 22.360371106517903 26.669456974380743 27.768454711718892 23.20171720738246 66-67 22.314583656133056 26.74558151006132 27.650757847421946 23.289076986383677 68-69 22.98955406982203 26.21727023427854 27.80646486571313 22.9867108301863 70-71 22.721066365037128 26.492456800362696 27.376142599302245 23.41033423529793 72-73 22.657181377394657 26.662002926518984 27.404035722133656 23.276779973952703 74-75 22.65599825793111 27.032373299973067 27.179801169637045 23.131827272458782 76-77 22.69954998022981 26.581692319424555 27.612475188081287 23.10628251226435 78-79 22.763995949866146 26.780518274311493 27.525063178649784 22.930422597172576 80-81 23.06216278167648 26.751992453362483 27.174154529745742 23.01169023521529 82-83 22.78113766720498 26.933553977378548 27.308491629198983 22.97681672621749 84-85 22.676433992851937 27.041401663472485 27.279934845307196 23.002229498368376 86-87 22.978044818458134 27.030418980068195 26.924731704199058 23.066804497274614 88-89 22.710261588042144 27.312682411407728 26.541821334789763 23.43523466576037 90-91 22.720116411802785 27.088666798765793 26.899092064640996 23.292124724790426 92-93 22.855353181105077 26.709372039706125 27.07292753531194 23.36234724387686 94-95 22.564491717567016 26.480973777934214 27.288620368854076 23.665914135644687 96-97 22.91946487710502 26.13652342914876 27.127943989212554 23.816067704533666 98-99 22.55403962060631 26.52436538131781 26.797819945366392 24.123775052709487 100-101 23.05984350473228 25.748060339412504 27.108148007089937 24.083948148765277 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 640.0 1 454.0 2 404.0 3 569.0 4 465.0 5 423.5 6 849.0 7 1752.5 8 2719.0 9 3134.5 10 2652.5 11 2032.0 12 1950.5 13 2091.0 14 2287.5 15 2487.0 16 2632.0 17 2848.0 18 3130.5 19 3451.5 20 3922.0 21 4612.5 22 5579.0 23 6863.0 24 8398.0 25 10441.0 26 13400.0 27 16703.5 28 19492.0 29 22032.0 30 24722.5 31 29277.0 32 36733.5 33 38966.5 34 37552.5 35 39541.0 36 43266.0 37 46796.5 38 50033.0 39 53277.5 40 56339.5 41 60379.0 42 63378.0 43 65744.5 44 68832.0 45 73860.5 46 77337.0 47 79897.5 48 82415.5 49 82344.5 50 81981.0 51 81511.0 52 81911.0 53 84524.0 54 86823.0 55 83414.0 56 77073.5 57 71428.5 58 66655.5 59 59493.5 60 48828.5 61 40736.5 62 34978.0 63 27383.5 64 20044.0 65 15163.5 66 11515.0 67 8892.0 68 6878.0 69 5246.0 70 4026.0 71 2935.0 72 2152.0 73 1729.5 74 1334.5 75 1039.0 76 781.5 77 519.5 78 323.5 79 182.5 80 109.5 81 67.5 82 34.0 83 22.5 84 14.0 85 9.0 86 7.5 87 6.0 88 2.5 89 0.5 90 0.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.012122189911076024 3 0.0025125993633866666 4 0.0018073083140149708 5 0.11593221624047251 6 0.0 7 0.0 8 3.9672621527157896E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 4.408069058573099E-5 16-17 4.408069058573099E-5 18-19 0.0 20-21 0.0 22-23 2.20403452928655E-4 24-25 4.8488759644304093E-4 26-27 0.005928852883780819 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.010447123668818245 36-37 7.273313946645613E-4 38-39 0.006061094955538012 40-41 0.009411227440053568 42-43 0.005532126668509239 44-45 0.003680737663908538 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 4.408069058573099E-5 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 1.5428241705005847E-4 68-69 0.0012342593364004677 70-71 2.2040345292865497E-5 72-73 6.612103587859648E-5 74-75 0.0 76-77 0.0 78-79 0.0 80-81 3.0856483410011693E-4 82-83 0.002490559018093801 84-85 0.0052676425249948535 86-87 0.0036146166280299416 88-89 0.0 90-91 0.0030195273051225728 92-93 0.0 94-95 2.2040345292865497E-5 96-97 1.7632276234292397E-4 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2268567.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.46582319367009 #Duplication Level Percentage of deduplicated Percentage of total 1 76.39676087272184 40.84615709390039 2 13.015615551288368 13.917811996439337 3 4.365167657240445 7.001618465182342 4 2.007929344203483 4.294223812102615 5 1.0885830561028629 2.910099460461036 6 0.6682881156380417 2.14383445398807 7 0.46439470279645695 1.738047155025464 8 0.32656332090012424 1.3967981421427034 9 0.25110367276841666 1.2082918114365622 >10 1.288853560354083 12.407140532284052 >50 0.0824302821042111 3.010040858208478 >100 0.03761770579710543 3.8436340231711927 >500 0.0033047694245173145 1.223505261789684 >1k 0.003222150188904 3.3674444588780714 >5k 1.6523847122584614E-4 0.691352474989843 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8398 0.37018963953896883 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7253 0.3197172488183069 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4620 0.20365279050607718 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 4075 0.1796288141368538 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 3922 0.17288446847723696 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 3736 0.164685460028291 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 3561 0.15697133917578807 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 2857 0.12593853300343344 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 2669 0.11765136317331602 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 2645 0.11659342659925848 No Hit GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA 2581 0.1137722624017717 No Hit CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG 2467 0.10874706367499835 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 4.408069058573099E-5 0.0 4 0.0 0.0 0.0 4.408069058573099E-5 0.0 5 0.0 0.0 0.0 4.408069058573099E-5 4.408069058573099E-5 6 0.0 0.0 0.0 4.408069058573099E-5 8.816138117146199E-5 7 0.0 0.0 0.0 4.408069058573099E-5 8.816138117146199E-5 8 0.0 0.0 0.0 4.408069058573099E-5 8.816138117146199E-5 9 0.0 0.0 0.0 4.408069058573099E-5 1.7632276234292397E-4 10-11 0.0 0.0 0.0 4.408069058573099E-5 2.4244379822152047E-4 12-13 0.0 0.0 0.0 4.408069058573099E-5 4.408069058573099E-4 14-15 4.408069058573099E-5 0.0 0.0 4.408069058573099E-5 5.28968287028772E-4 16-17 4.408069058573099E-5 0.0 0.0 4.408069058573099E-5 6.171296682002339E-4 18-19 4.408069058573099E-5 0.0 0.0 6.61210358785965E-5 6.171296682002339E-4 20-21 4.408069058573099E-5 0.0 0.0 8.816138117146199E-5 7.273313946645613E-4 22-23 4.408069058573099E-5 0.0 0.0 1.9836310763578945E-4 7.493717399574269E-4 24-25 4.408069058573099E-5 0.0 0.0 2.4244379822152047E-4 7.493717399574269E-4 26-27 4.408069058573099E-5 0.0 0.0 5.069279417359064E-4 7.714120852502924E-4 28-29 4.408069058573099E-5 0.0 0.0 0.002799123852193918 8.154927758360234E-4 30-31 4.408069058573099E-5 0.0 0.0 0.011703423350511579 8.375331211288888E-4 32-33 4.408069058573099E-5 0.0 0.0 0.02975446614536842 9.697751928860819E-4 34-35 4.408069058573099E-5 0.0 0.0 0.05466005632630643 0.001057936574057544 36-37 4.408069058573099E-5 0.0 0.0 0.0979252541362014 0.0011020172646432748 38-39 4.408069058573099E-5 0.0 0.0 0.16413445139596936 0.0011460979552290057 40-41 4.408069058573099E-5 0.0 0.0 0.23600801739600372 0.0011901786458147368 42-43 4.408069058573099E-5 0.0 0.0 0.30600815404614456 0.0011901786458147368 44-45 4.408069058573099E-5 0.0 0.0 0.3918773393071485 0.0011901786458147368 46-47 8.816138117146199E-5 0.0 0.0 0.49191846659146504 0.0011901786458147368 48-49 8.816138117146199E-5 0.0 0.0 0.5940313863333109 0.0011901786458147368 50-51 8.816138117146199E-5 0.0 0.0 0.7059743000757748 0.0012342593364004677 52-53 8.816138117146199E-5 0.0 0.0 0.8102471736563214 0.0013224207175719298 54-55 8.816138117146199E-5 0.0 0.0 0.9231378222463784 0.0013444610628647953 56-57 8.816138117146199E-5 0.0 0.0 1.0561513060888217 0.0014326224440362571 58-59 8.816138117146199E-5 0.0 0.0 1.1940136658956955 0.0014987434799148538 60-61 8.816138117146199E-5 0.0 0.0 1.3437998525060093 0.0014987434799148538 62-63 8.816138117146199E-5 0.0 0.0 1.4907207942282508 0.0014987434799148538 64-65 8.816138117146199E-5 0.0 0.0 1.6399118915156574 0.0015428241705005847 66-67 8.816138117146199E-5 0.0 0.0 1.8143612245086875 0.0015869048610863156 68-69 8.816138117146199E-5 0.0 0.0 2.0016380384621657 0.0016309855516720467 70-71 1.32242071757193E-4 0.0 0.0 2.194314737012396 0.0016750662422577777 72-73 1.32242071757193E-4 0.0 0.0 2.395146363320986 0.0017191469328435086 74-75 1.32242071757193E-4 0.0 0.0 2.593487430611483 0.0017852679687221052 76-77 1.32242071757193E-4 0.0 0.0 2.806044520615878 0.0018073083140149706 78-79 1.32242071757193E-4 0.0 0.0 3.0509347971649063 0.0018293486593078361 80-81 1.32242071757193E-4 0.0 0.0 3.3008723127860007 0.0018513890046007017 82-83 1.32242071757193E-4 0.0 0.0 3.55092003013356 0.0018513890046007017 84-85 1.32242071757193E-4 0.0 0.0 3.806191309315528 0.0018513890046007017 86-87 1.32242071757193E-4 0.0 0.0 4.06018424847051 0.001961590731065029 88-89 1.32242071757193E-4 0.0 0.0 4.332691077671499 0.0020277117669436255 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3910 0.0 54.732784 1 GTATCAA 7135 0.0 44.190746 1 ATCAACG 9060 0.0 34.586887 3 TATCAAC 9135 0.0 34.411587 2 TCAACGC 9160 0.0 34.2093 4 CAACGCA 9305 0.0 33.675476 5 AACGCAG 9605 0.0 32.68517 6 TGGTATC 1680 0.0 29.722942 2 ACGCAGA 11060 0.0 28.213497 7 CGCAGAG 11085 0.0 28.149864 8 GTGGTAT 1825 0.0 28.143145 1 GCAGAGT 12435 0.0 24.941011 9 GTACATG 7015 0.0 23.930887 1 TACATGG 6815 0.0 23.865582 2 GAGTACT 8615 0.0 22.162432 12-13 CAGAGTA 11875 0.0 21.497675 10-11 ACATGGG 7170 0.0 21.420897 3 GTACTTT 8940 0.0 21.303625 14-15 GGACCGA 470 0.0 21.22111 6 AGAGTAC 11390 0.0 20.995317 10-11 >>END_MODULE