FastQCFastQC Report
Thu 26 May 2016
SRR937930_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937930_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2222093
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86460.3890926257361866No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69850.31434327906167747No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42880.19297122127651722No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA41970.1888759831384195No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA40760.18343066649325657No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA36250.1631344862703766No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA35390.15926426121678974No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA27440.12348718077956233No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA27110.12200209442179062No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG27020.12159707086967107No Hit
CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG24610.11075144019624741No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC24330.10949136692298657No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA36700.053.591491
GTATCAA66350.042.1620181
TATCAAC85050.032.803962
ATCAACG84750.032.6957473
TCAACGC85300.032.596374
CAACGCA86350.032.200015
AACGCAG89200.031.2721256
ACGCAGA103950.026.6887
CGCAGAG105100.026.395988
GCAGAGT115150.023.9679079
TAAGGTG13600.023.7647065
GTACATG72300.023.5719011
TGGTATC17750.023.5638772
TACATGG71000.023.0952782
GTGGTAT19600.022.4076561
GTACCGT1703.139201E-622.3627536
GAGTACT77350.021.9550112-13
ACATGGG70600.021.947013
GAGTACA52750.021.9006791
GTGTAAG9750.021.5433271
Produced by FastQC (version 0.11.5)