##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937930_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2222093 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.08249789725272 33.0 31.0 34.0 31.0 34.0 2 32.2754349165404 34.0 31.0 34.0 31.0 34.0 3 32.36809170453262 34.0 31.0 34.0 31.0 34.0 4 35.88697097736233 37.0 35.0 37.0 35.0 37.0 5 35.89151894182647 37.0 35.0 37.0 35.0 37.0 6 35.9214996852067 37.0 37.0 37.0 35.0 37.0 7 35.87104635134533 37.0 36.0 37.0 35.0 37.0 8 35.84127667023837 37.0 36.0 37.0 35.0 37.0 9 37.5201942492956 39.0 38.0 39.0 35.0 39.0 10-11 37.44356761845701 39.0 37.0 39.0 35.0 39.0 12-13 37.348497790146496 39.0 37.0 39.0 35.0 39.0 14-15 38.59736271164168 40.0 38.0 41.0 34.5 41.0 16-17 38.45260189380012 40.0 38.0 41.0 34.0 41.0 18-19 38.579326562839626 40.0 38.0 41.0 34.0 41.0 20-21 38.54878261170887 40.0 38.0 41.0 34.0 41.0 22-23 38.468561846871395 40.0 38.0 41.0 34.0 41.0 24-25 38.36412854907513 40.0 38.0 41.0 34.0 41.0 26-27 38.19607797693436 40.0 38.0 41.0 34.0 41.0 28-29 37.995367655629174 40.0 38.0 41.0 33.5 41.0 30-31 37.78508775285283 40.0 37.5 41.0 33.0 41.0 32-33 37.53821599726024 40.0 37.0 41.0 33.0 41.0 34-35 37.31128175103382 40.0 37.0 41.0 32.0 41.0 36-37 37.05503910052369 39.5 36.5 41.0 31.0 41.0 38-39 36.76446260350039 39.0 36.0 41.0 30.5 41.0 40-41 36.5552467876007 39.0 36.0 41.0 30.0 41.0 42-43 36.390596838206136 39.0 35.5 41.0 30.0 41.0 44-45 36.1391831935027 39.0 35.0 41.0 29.5 41.0 46-47 35.77583476479157 39.0 35.0 40.0 28.5 41.0 48-49 35.389571453580025 39.0 35.0 40.0 27.0 41.0 50-51 34.67918826979789 37.5 34.0 39.5 26.0 40.5 52-53 34.79391344106659 38.0 34.0 40.0 26.0 40.5 54-55 34.892631631529376 38.0 34.0 40.0 26.0 41.0 56-57 34.579329713022815 38.0 34.0 40.0 25.5 41.0 58-59 34.24681527730837 38.0 34.0 40.0 24.5 41.0 60-61 33.81863022834778 37.0 33.5 40.0 23.0 41.0 62-63 33.26721091331461 36.5 33.0 39.5 21.5 41.0 64-65 32.74479173463937 36.0 32.0 39.0 19.5 40.5 66-67 32.213157595114154 35.0 31.5 39.0 17.5 40.0 68-69 31.602455207770333 35.0 31.0 38.0 11.5 40.0 70-71 31.045254631556823 35.0 30.5 37.5 7.5 39.5 72-73 30.409667372157692 34.5 30.0 37.0 4.0 39.0 74-75 29.740311049087502 34.0 29.0 36.0 2.0 39.0 76-77 29.086294768040762 34.0 28.5 35.5 2.0 38.0 78-79 28.375903708800667 34.0 27.0 35.0 2.0 37.0 80-81 27.740718547783555 33.0 26.0 35.0 2.0 37.0 82-83 27.017637650629382 33.0 25.0 35.0 2.0 36.0 84-85 26.38182537814574 32.0 24.0 35.0 2.0 36.0 86-87 25.70727012775793 32.0 21.5 34.5 2.0 35.0 88-89 24.892034671816166 31.0 19.0 34.0 2.0 35.0 90-91 24.061767666789823 31.0 11.0 34.0 2.0 35.0 92-93 23.164499640654107 30.5 3.5 34.0 2.0 35.0 94-95 22.214371315692006 29.5 2.0 34.0 2.0 35.0 96-97 20.999875567764263 29.0 2.0 34.0 2.0 35.0 98-99 19.483068665442897 28.0 2.0 34.0 2.0 35.0 100-101 15.720015768916962 13.0 2.0 30.0 2.0 33.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3438233304975711 1101 2 0.3156188346390678 1101 3 0.25364421615042687 1101 4 0.22102409923019195 1101 5 0.32721866613989903 1101 6 0.29397677465441774 1101 7 0.25902837514006194 1101 8 0.2623260315053457 1101 9 0.39275582283082144 1101 10-11 0.34414391915851894 1101 12-13 0.34730827079838633 1101 14-15 0.46283713720065833 1101 16-17 0.6137360563125114 1101 18-19 0.48017905432676145 1101 20-21 0.5873832631700608 1101 22-23 0.5645532928795021 1101 24-25 0.5587295457039403 1101 26-27 0.6167545422573255 1101 28-29 0.6394884525770337 1101 30-31 0.7198012362147068 1101 32-33 0.632096347224234 1101 34-35 0.6429676265736575 1101 36-37 0.6942655531695721 1101 38-39 0.6567847128424305 1101 40-41 0.6213250993267323 1101 42-43 0.8510379710169218 1101 44-45 0.8163149014818103 1101 46-47 0.8306894687729525 1101 48-49 1.1123136554138853 1101 50-51 0.9796354534759715 1101 52-53 1.0117900206386778 1101 54-55 1.0105156472542731 1101 56-57 1.0794100396456017 1101 58-59 1.1765955363370821 1101 60-61 1.224290523482928 1101 62-63 1.0952206573942789 1101 64-65 1.227241548533648 1101 66-67 1.3768054392631726 1101 68-69 1.585319085853314 1101 70-71 1.8941731859377313 1101 72-73 1.9332236729810042 1101 74-75 2.0620534146216585 1101 76-77 2.1057925753158138 1101 78-79 2.2446140057920942 1101 80-81 2.4492766440384592 1101 82-83 2.420258602186532 1101 84-85 2.6274763949982862 1101 86-87 2.8811423583971028 1101 88-89 3.258357845412789 1101 90-91 3.510661892816561 1101 92-93 3.5754197279593356 1101 94-95 3.904479199913208 1101 96-97 4.133828600557344 1101 98-99 4.534765121116735 1101 100-101 4.335837268664941 1102 1 0.31938058979764605 1102 2 0.1644592395823281 1102 3 0.12823085476951945 1102 4 0.09038328153555852 1102 5 0.23166107294015603 1102 6 0.20718533224633262 1102 7 0.10931225407831135 1102 8 0.23923173181497503 1102 9 0.27076493315512806 1102 10-11 0.253598994883113 1102 12-13 0.3337557965375524 1102 14-15 0.43945067529154613 1102 16-17 0.38394661858672663 1102 18-19 0.2638226575152416 1102 20-21 0.3487666737498003 1102 22-23 0.4032630531523793 1102 24-25 0.37914963002644697 1102 26-27 0.3995213377322173 1102 28-29 0.39944636837523717 1102 30-31 0.4417915479871084 1102 32-33 0.531355603629649 1102 34-35 0.556708497306964 1102 36-37 0.5989818219177181 1102 38-39 0.5625925766935467 1102 40-41 0.8531452250014411 1102 42-43 0.8877534512481304 1102 44-45 0.7487297538532118 1102 46-47 0.7800404191670225 1102 48-49 0.8918984511636054 1102 50-51 1.0208537635069561 1102 52-53 0.9773611110908078 1102 54-55 1.0744702779933348 1102 56-57 1.0544263172549293 1102 58-59 1.085417807959331 1102 60-61 1.0746681511770788 1102 62-63 1.0522946431418916 1102 64-65 0.9931781949223293 1102 66-67 1.029597932171022 1102 68-69 1.307437178464241 1102 70-71 1.4908252637569035 1102 72-73 1.6898014966485881 1102 74-75 1.724182256233778 1102 76-77 1.8193397200240788 1102 78-79 1.9621849626843613 1102 80-81 2.0309918481603226 1102 82-83 2.3355164690555945 1102 84-85 2.4087406419196675 1102 86-87 2.6493954338057755 1102 88-89 2.8862231220294134 1102 90-91 3.000386130208959 1102 92-93 3.2602714058694318 1102 94-95 3.643527932571292 1102 96-97 3.676361961630036 1102 98-99 3.9891682304932807 1102 100-101 3.8502691190984333 1103 1 0.35419259824845284 1103 2 0.2769671054506233 1103 3 0.21303518656535658 1103 4 0.22002468443884737 1103 5 0.1472816602150715 1103 6 0.13431171161148114 1103 7 0.2784154477830185 1103 8 0.11185710456523168 1103 9 0.17946463754984876 1103 10-11 0.22571543784555814 1103 12-13 0.3593263558088964 1103 14-15 0.34028505200122794 1103 16-17 0.3971761440829553 1103 18-19 0.36268959405418144 1103 20-21 0.27855852827826766 1103 22-23 0.37789846610235855 1103 24-25 0.41034766393779876 1103 26-27 0.3816958205310996 1103 28-29 0.43041275111378496 1103 30-31 0.41062703186505445 1103 32-33 0.5053593143732868 1103 34-35 0.48276960564294313 1103 36-37 0.5235765269719863 1103 38-39 0.6957552396062212 1103 40-41 0.7604771873490677 1103 42-43 0.8589059874635581 1103 44-45 0.8815864317861823 1103 46-47 0.8937211373559322 1103 48-49 0.9430630862197447 1103 50-51 0.7591236184722376 1103 52-53 0.9021936843271803 1103 54-55 0.9468575122567273 1103 56-57 1.063050390379935 1103 58-59 1.0741977279923702 1103 60-61 1.296034228146901 1103 62-63 1.1924108958098003 1103 64-65 1.3379343367869083 1103 66-67 1.4104448942699648 1103 68-69 1.7199211493924267 1103 70-71 1.7525564900106332 1103 72-73 1.7767601573209753 1103 74-75 1.8434539579211275 1103 76-77 1.895386718451988 1103 78-79 2.203295179987755 1103 80-81 2.2016306431633588 1103 82-83 2.2449792437663127 1103 84-85 2.1005740325576063 1103 86-87 2.2409695481252214 1103 88-89 2.5748209647812423 1103 90-91 2.6258732193727674 1103 92-93 2.7945817286998036 1103 94-95 3.352890586401731 1103 96-97 3.580833559087875 1103 98-99 3.8600660855710096 1103 100-101 3.670994357561286 1104 1 0.27588059234836493 1104 2 0.1788341225925194 1104 3 0.24941855684058822 1104 4 0.2252395130845244 1104 5 0.20351661265507914 1104 6 0.18503349684554138 1104 7 0.17361102749540436 1104 8 0.17381672360753697 1104 9 0.28936420270709107 1104 10-11 0.2438190397051656 1104 12-13 0.24981980629465284 1104 14-15 0.42744992498713685 1104 16-17 0.4281153954845749 1104 18-19 0.3139336335035452 1104 20-21 0.3698910590750657 1104 22-23 0.26739065356347425 1104 24-25 0.32245249597743 1104 26-27 0.4397787800496218 1104 28-29 0.44243475003688815 1104 30-31 0.4377442403517051 1104 32-33 0.291629221442939 1104 34-35 0.346131167095713 1104 36-37 0.4159270665691537 1104 38-39 0.4786927870691713 1104 40-41 0.44160128922310093 1104 42-43 0.40697333086194476 1104 44-45 0.4223106405544925 1104 46-47 0.5668728316900413 1104 48-49 0.7316795168862669 1104 50-51 0.6798128377757138 1104 52-53 0.5774833887356294 1104 54-55 0.6938769881164433 1104 56-57 0.7852470529692184 1104 58-59 0.7456927599959613 1104 60-61 0.8374754198688592 1104 62-63 1.0147966886240027 1104 64-65 1.168129309371615 1104 66-67 1.2083403011002503 1104 68-69 1.0624936887089191 1104 70-71 1.25651376812786 1104 72-73 1.2664872584057427 1104 74-75 1.4330233302207773 1104 76-77 1.6010681396483086 1104 78-79 1.7926238162097903 1104 80-81 1.9173337766388876 1104 82-83 1.973283760099541 1104 84-85 2.0456091961984235 1104 86-87 2.177151595857403 1104 88-89 2.286143323701193 1104 90-91 2.500677893459663 1104 92-93 2.719500189461936 1104 94-95 2.762711847017755 1104 96-97 3.042500413201065 1104 98-99 3.3466118625003567 1104 100-101 3.373181559906987 1105 1 0.2605944409153338 1105 2 0.2263612930951382 1105 3 0.16673056278145992 1105 4 0.05516565973440635 1105 5 -0.01293656283429101 1105 6 0.10874673692539716 1105 7 0.19228308921096726 1105 8 0.12677361366923634 1105 9 0.2634418258404452 1105 10-11 0.1476333687237812 1105 12-13 0.1925658193933728 1105 14-15 0.28591059802881347 1105 16-17 0.2912216853811884 1105 18-19 0.23043157023129623 1105 20-21 0.23700341696531524 1105 22-23 0.33334273839042083 1105 24-25 0.3368725093680922 1105 26-27 0.28421004569383257 1105 28-29 0.3220429087556056 1105 30-31 0.38754818325482177 1105 32-33 0.43320724092782115 1105 34-35 0.583946477521998 1105 36-37 0.5319758288481893 1105 38-39 0.633208923109585 1105 40-41 0.7200530890180943 1105 42-43 0.854530593556909 1105 44-45 0.9181653333295472 1105 46-47 0.9468127047605961 1105 48-49 0.9453155891657019 1105 50-51 0.9580216197762752 1105 52-53 0.8777791396857495 1105 54-55 0.9628887091690004 1105 56-57 1.0609574289043806 1105 58-59 0.9141864141522831 1105 60-61 1.1594061284663795 1105 62-63 1.1502914529236676 1105 64-65 1.2168647460342825 1105 66-67 1.4885358680319527 1105 68-69 1.6817907379683703 1105 70-71 1.8131124957452158 1105 72-73 1.8779257747242077 1105 74-75 1.9029877851651733 1105 76-77 2.0636883760012914 1105 78-79 2.2414607152704136 1105 80-81 2.350510167338964 1105 82-83 2.6065559933549665 1105 84-85 2.7932678494722687 1105 86-87 2.9576148683905004 1105 88-89 3.061052288535162 1105 90-91 3.1698632550803723 1105 92-93 3.421738754114635 1105 94-95 3.6368505343194784 1105 96-97 3.8093543301505655 1105 98-99 3.924231315127688 1105 100-101 3.7723472197599985 1106 1 0.12054860608280649 1106 2 0.07365577379037092 1106 3 0.07651200993904439 1106 4 0.05080746084235699 1106 5 0.11514164811615046 1106 6 0.0050613657140345936 1106 7 0.07957932031706605 1106 8 0.06306460110625522 1106 9 0.04924448106245194 1106 10-11 0.04063734388524409 1106 12-13 0.19664849065000567 1106 14-15 0.2743219597080042 1106 16-17 0.21616471599069342 1106 18-19 0.21198337428901226 1106 20-21 0.1895937549134672 1106 22-23 0.101659923643318 1106 24-25 0.12016516185643411 1106 26-27 0.05449131078707836 1106 28-29 0.06593591975401836 1106 30-31 0.10575661612926268 1106 32-33 0.20123809353998467 1106 34-35 0.28167915520760545 1106 36-37 0.38477032737169026 1106 38-39 0.2544302776898846 1106 40-41 0.4858051660102376 1106 42-43 0.36981049181697756 1106 44-45 0.5155143793016137 1106 46-47 0.7514811693801491 1106 48-49 0.8340502366037796 1106 50-51 0.8516968558926479 1106 52-53 0.8591756217921187 1106 54-55 0.4781372729136706 1106 56-57 0.8290223172832683 1106 58-59 0.9605639280718279 1106 60-61 1.0686113987157668 1106 62-63 1.0819927442298578 1106 64-65 1.1871282909188139 1106 66-67 1.400215214372345 1106 68-69 1.1414842864077457 1106 70-71 1.392603028939817 1106 72-73 1.527298220015762 1106 74-75 1.584258226884593 1106 76-77 1.7577130459894157 1106 78-79 2.054687554471407 1106 80-81 2.184953444842833 1106 82-83 2.309397277551458 1106 84-85 2.4727813973281734 1106 86-87 2.4034183123778945 1106 88-89 2.689977611901959 1106 90-91 2.8195266251399183 1106 92-93 3.0786863185043636 1106 94-95 3.3385672875882655 1106 96-97 3.5149762824637527 1106 98-99 3.7954129613134633 1106 100-101 4.032958815475601 1107 1 0.16309530387246696 1107 2 0.0860463071527704 1107 3 -0.0492915429744869 1107 4 -0.014599220676856817 1107 5 0.07171017808621372 1107 6 0.12813775707687114 1107 7 0.15033309593017918 1107 8 0.21277561450013138 1107 9 0.23523204249758578 1107 10-11 0.15632521353730766 1107 12-13 0.0751227548020168 1107 14-15 0.14181801173091202 1107 16-17 0.2174998500624028 1107 18-19 0.15801536929798488 1107 20-21 0.2605550762584201 1107 22-23 0.2218203063356725 1107 24-25 0.16022715971893575 1107 26-27 0.18851587243312196 1107 28-29 0.2837977024495686 1107 30-31 0.32160727891087504 1107 32-33 0.3273283289652582 1107 34-35 0.3044994376186807 1107 36-37 0.37733917991496924 1107 38-39 0.439413431215236 1107 40-41 0.41083090983393333 1107 42-43 0.5615660876646444 1107 44-45 0.5046919593999064 1107 46-47 0.5441083332349379 1107 48-49 0.6443074444313126 1107 50-51 0.5335119682704246 1107 52-53 0.4393664362540335 1107 54-55 0.5620694049276409 1107 56-57 0.7201605044572617 1107 58-59 0.6229684632385144 1107 60-61 0.9350851417879724 1107 62-63 0.9903012199296342 1107 64-65 0.975897425979511 1107 66-67 1.1707873210058395 1107 68-69 1.1486700478134537 1107 70-71 1.3719695979483362 1107 72-73 1.3562496089415745 1107 74-75 1.5444015592785654 1107 76-77 1.6939742629411718 1107 78-79 1.7815350393751572 1107 80-81 2.0984470190780975 1107 82-83 2.176333346022055 1107 84-85 2.2554344911065876 1107 86-87 2.327895327264116 1107 88-89 2.4972183170966282 1107 90-91 2.674126395084958 1107 92-93 2.778464593730206 1107 94-95 3.0638845879695005 1107 96-97 3.199181361042232 1107 98-99 3.442979926271736 1107 100-101 3.5653973626200273 1108 1 0.22838807322428067 1108 2 0.1394949871697193 1108 3 0.06918856074547364 1108 4 0.08763704908708547 1108 5 0.12714921064701912 1108 6 0.12695684298083165 1108 7 0.09368723633228626 1108 8 0.0737116001593634 1108 9 0.2242669473996486 1108 10-11 0.12782455846838303 1108 12-13 0.17157532431732392 1108 14-15 0.24572941674932736 1108 16-17 0.32891322199237294 1108 18-19 0.2945800634272544 1108 20-21 0.2704101768884257 1108 22-23 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-2.721167300816827 2316 96-97 -3.1504407954263947 2316 98-99 -3.589670326917936 2316 100-101 -3.3580712064916955 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4860.0 3 1381.0 4 1461.0 5 1634.0 6 1879.0 7 2275.0 8 2852.0 9 3725.0 10 4745.0 11 5851.0 12 7972.0 13 10007.0 14 11919.0 15 14683.0 16 17996.0 17 22422.0 18 22075.0 19 19008.0 20 19318.0 21 20408.0 22 22644.0 23 25713.0 24 29176.0 25 32614.0 26 37365.0 27 43626.0 28 51001.0 29 60178.0 30 72156.0 31 88719.0 32 109211.0 33 139820.0 34 184879.0 35 252786.0 36 330598.0 37 356320.0 38 178323.0 39 10493.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.76635349248061 17.590449897630332 13.618985923209864 24.024210686679197 2 15.936807245079695 18.924466190408445 39.79828057215816 25.340445992353693 3 19.784946042898227 22.506012118341364 29.03309467966362 28.675947159096793 4 13.080242683167345 15.354429273994771 35.47606804278385 36.08926000005403 5 14.128750686856495 36.66102168214533 33.96737319052723 15.242854440470936 6 34.387344835926136 34.65050616782964 16.241148199508988 14.72100079673523 7 30.002201644524906 32.07769058745188 19.39671338145135 18.52339438657186 8 27.41970628753568 34.27323722999073 18.843878589243747 19.463177893229847 9 26.544683418352417 16.37152363500475 19.507894843476343 37.575898103166494 10-11 25.8573398085005 25.69662377003491 26.357452820758958 22.08858360070563 12-13 26.52872725897485 22.916051354826624 27.060626825675254 23.494594560523275 14-15 22.926373580611795 24.570494733252374 25.175335031328117 27.327796654807717 16-17 22.811938938500905 27.770011831250706 26.260581946267752 23.157467283980633 18-19 22.956730688058308 26.030158197378768 28.496239061220695 22.51687205334223 20-21 23.02004500473349 25.272668123298107 29.074633787770825 22.632653084197575 22-23 22.781581529121546 25.28962613679187 28.863516539227113 23.065275794859467 24-25 22.72216877187002 25.5187842650469 28.937447777079534 22.82159918600355 26-27 22.18075642116416 25.939695326924795 28.74381512036327 23.135733131547777 28-29 22.426317147260352 26.453768323633213 28.48518779149316 22.63472673761327 30-31 22.527988445107393 25.828588769143252 28.48186027287538 23.161562512873974 32-33 22.05159340110162 25.911125938612344 28.599210840764343 23.438069819521694 34-35 23.03113954092022 26.062022439127837 28.392293244465915 22.51454477548603 36-37 23.180315454459095 25.550523543826902 28.27035364702291 22.998807354691095 38-39 22.552233263186324 25.956561371130192 28.3003792439878 23.190826121695682 40-41 23.027198880787484 25.799887646619812 28.43080679269811 22.742106679894597 42-43 22.643528660043568 25.77217402929507 28.30954796127151 23.274749349389857 44-45 22.676036464073228 26.14859422657425 28.472102040352453 22.70326726900007 46-47 23.193651652504634 26.370796377118705 27.709694140175646 22.725857830201015 48-49 22.827176604625976 26.519953418840164 27.543248950370895 23.109621026162966 50-51 22.99489947941563 26.783314294150422 27.561962863241053 22.659823363192892 52-53 23.396515580640916 26.268106524256247 27.62100553730791 22.714372357794936 54-55 22.669706037835805 26.65547119442651 27.789294070863196 22.885528696874488 56-57 22.84580111029394 26.798601465328748 27.523569216033817 22.832028208343498 58-59 22.772037032327784 26.275475815992387 27.52885794484939 23.423629206830444 60-61 22.35439712348926 26.622847753733087 27.94941035535764 23.07334476742001 62-63 22.645088170505886 26.59138108436804 28.01374881960717 22.7497819255189 64-65 22.298177127288717 26.67959362888006 27.860574250589522 23.1616549932417 66-67 22.254824478891468 26.682570487034056 27.75640465943981 23.30620037463466 68-69 22.942654727770805 26.174586660851595 27.939158709765692 22.943599901611904 70-71 22.68821153635815 26.423027705497937 27.50409710810109 23.384663650042828 72-73 22.637934957340608 26.55376348078848 27.54166635722264 23.266635204648278 74-75 22.602043170098018 26.90854343958763 27.463770806679744 23.02564258363461 76-77 22.805879342773082 26.540912857169218 27.49685964279815 23.156348157259547 78-79 22.864213722880574 26.73754935765843 27.43297867983201 22.965258239628987 80-81 23.113208502582367 26.73997834542579 27.09952259563222 23.04729055635962 82-83 22.899077398929318 26.884967876847295 27.167716434073498 23.04823829014989 84-85 22.83004010919928 27.061996089223673 27.117108825036528 22.99085497654052 86-87 23.083275578077604 27.052726798138632 26.806869425117686 23.05712819866608 88-89 22.922027747185904 27.14467416672125 26.584319569578525 23.348978516514325 90-91 22.9521546878007 27.02633838687931 26.683398026674887 23.338108898645096 92-93 23.237496255690168 26.536449829399007 26.989997994782833 23.236055920127992 94-95 22.937090482653684 26.359873321133627 27.178514111818636 23.524522084394057 96-97 23.353416732444693 25.937339704742747 27.011530342408115 23.697713220404445 98-99 23.00028425977337 26.32972468214333 26.645358812131747 24.02463224595155 100-101 23.864793064152266 25.2661640356924 26.789313011159454 24.079729888995878 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 601.0 1 454.0 2 440.0 3 594.0 4 498.0 5 453.5 6 846.5 7 1695.5 8 2711.5 9 3145.0 10 2552.0 11 1953.0 12 1918.5 13 1992.5 14 2128.5 15 2301.5 16 2444.0 17 2663.5 18 2944.5 19 3223.5 20 3630.5 21 4353.5 22 5288.5 23 6489.0 24 7995.5 25 9895.5 26 12630.0 27 15739.0 28 18390.0 29 20890.5 30 23547.0 31 28174.5 32 35809.0 33 38171.0 34 36794.5 35 38617.5 36 42010.0 37 45551.5 38 48964.0 39 51932.5 40 54859.5 41 59429.0 42 61882.0 43 63801.5 44 67306.0 45 72469.0 46 76232.0 47 78555.0 48 80742.0 49 80542.0 50 80465.0 51 80369.5 52 80345.5 53 82943.5 54 85397.5 55 81857.0 56 76011.0 57 70601.5 58 66010.5 59 59505.5 60 48910.5 61 40425.5 62 34764.0 63 27095.0 64 19585.0 65 14954.5 66 11327.0 67 8644.5 68 6654.5 69 5129.5 70 3947.0 71 2874.5 72 2143.5 73 1722.5 74 1345.0 75 997.0 76 731.0 77 492.0 78 320.5 79 190.5 80 113.0 81 77.5 82 48.0 83 31.5 84 28.5 85 20.5 86 16.0 87 13.5 88 7.5 89 5.5 90 5.0 91 3.5 92 2.5 93 2.5 94 1.5 95 2.0 96 2.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4505662004245547 2 0.04000732642603167 3 0.052293040840324864 4 0.05647828421222694 5 0.08248979678168285 6 0.08019466331967204 7 0.046172684941629356 8 0.03928728455559691 9 0.04603767709092284 10-11 0.04927786550787928 12-13 0.04486760905146634 14-15 0.038049712590787155 16-17 0.038567242685162144 18-19 0.046735217652906516 20-21 0.05470068084459112 22-23 0.04680272157825978 24-25 0.0650062801151887 26-27 0.05641078028687368 28-29 0.04437258026554244 30-31 0.046915228120515205 32-33 0.04477760381766199 34-35 0.04979539560225427 36-37 0.04410256456412941 38-39 0.025561486400434184 40-41 0.02389638957505379 42-43 0.023603872565189667 44-45 0.015503401522798551 46-47 0.027069074066656977 48-49 0.026731554439890677 50-51 0.01615593946788006 52-53 0.015548404139700725 54-55 0.01626844601013549 56-57 0.015255887129836599 58-59 0.013703296846711635 60-61 0.016808477412961563 62-63 0.016875981338314822 64-65 0.01710099442282569 66-67 0.00877551029592371 68-69 0.012578231424157316 70-71 0.018181057228477838 72-73 0.016493459094646355 74-75 0.013523286379102944 76-77 0.009540554783260647 78-79 0.013500785070651857 80-81 0.017618524517200675 82-83 0.014895866194619217 84-85 0.012758241891766005 86-87 0.0032851910338586193 88-89 0.028914181359646064 90-91 0.03219937239350468 92-93 0.017483516666494156 94-95 0.032761905104781844 96-97 0.02729408715116784 98-99 0.024008896117309223 100-101 0.1485761397025237 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2222093.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.57500549845762 #Duplication Level Percentage of deduplicated Percentage of total 1 77.06445510816665 42.05793061263836 2 12.809770851049645 13.981866292600332 3 4.193442213760816 6.865713956203826 4 1.9129582833919478 4.1759883533774245 5 1.0473641480806402 2.8579952070194157 6 0.6486293293662004 2.1239369529972763 7 0.43189615715887897 1.6499514606195975 8 0.3202834126096945 1.3983575203391079 9 0.2360682465649648 1.1595083268864783 >10 1.2159955027320057 11.958324562712285 >50 0.07736853829509312 2.869238706133717 >100 0.03532067152274824 3.6829811633957688 >500 0.002975786446441346 1.1192763246562056 >1k 0.0033064293849346597 3.3937833180397057 >5k 1.6532146924673298E-4 0.7051472423804152 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8646 0.3890926257361866 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6985 0.31434327906167747 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4288 0.19297122127651722 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 4197 0.1888759831384195 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 4076 0.18343066649325657 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 3625 0.1631344862703766 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 3539 0.15926426121678974 No Hit GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA 2744 0.12348718077956233 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 2711 0.12200209442179062 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 2702 0.12159707086967107 No Hit CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG 2461 0.11075144019624741 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 2433 0.10949136692298657 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 9.000523380434572E-5 2 0.0 0.0 0.0 0.0 1.3500785070651858E-4 3 0.0 0.0 0.0 0.0 1.3500785070651858E-4 4 0.0 0.0 0.0 0.0 1.3500785070651858E-4 5 0.0 0.0 0.0 0.0 1.8001046760869144E-4 6 0.0 0.0 0.0 0.0 1.8001046760869144E-4 7 0.0 0.0 0.0 0.0 1.8001046760869144E-4 8 0.0 0.0 0.0 0.0 1.8001046760869144E-4 9 0.0 0.0 0.0 0.0 3.600209352173829E-4 10-11 0.0 0.0 0.0 0.0 4.0502355211955576E-4 12-13 0.0 0.0 0.0 0.0 5.400314028260743E-4 14-15 0.0 0.0 0.0 0.0 6.300366366304201E-4 16-17 0.0 0.0 0.0 0.0 7.425431788858522E-4 18-19 0.0 0.0 0.0 0.0 8.100471042391115E-4 20-21 0.0 0.0 0.0 6.750392535325929E-5 9.90057571847803E-4 22-23 0.0 0.0 0.0 9.000523380434572E-5 0.0010800628056521486 24-25 4.500261690217286E-5 0.0 0.0 2.7001570141303716E-4 0.0010800628056521486 26-27 4.500261690217286E-5 0.0 0.0 7.200418704347658E-4 0.001102564114103235 28-29 4.500261690217286E-5 0.0 0.0 0.0029026687901901496 0.0011250654225543216 30-31 4.500261690217286E-5 0.0 0.0 0.011003139832581265 0.001147566731005408 32-33 4.500261690217286E-5 0.0 0.0 0.02778911593709174 0.0012600732732608402 34-35 4.500261690217286E-5 0.0 0.0 0.054385662526275906 0.0013275771986140995 36-37 4.500261690217286E-5 0.0 0.0 0.09963579382141072 0.0013950811239673588 38-39 4.500261690217286E-5 0.0 0.0 0.16902982908456127 0.0014850863577717045 40-41 4.500261690217286E-5 0.0 0.0 0.24049398472521177 0.0014850863577717045 42-43 6.750392535325929E-5 0.0 0.0 0.3138932528926557 0.0014850863577717045 44-45 9.000523380434572E-5 0.0 0.0 0.3984981726687407 0.0015075876662227908 46-47 1.8001046760869144E-4 0.0 0.0 0.4985839926591732 0.0015750915915760501 48-49 2.250130845108643E-4 0.0 0.0 0.6048576724736543 0.0018001046760869146 50-51 2.250130845108643E-4 0.0 0.0 0.7211894371657712 0.0018901099098912603 52-53 2.250130845108643E-4 0.0 0.0 0.8280481509999806 0.001935112526793433 54-55 2.7001570141303716E-4 0.0 0.0 0.9446499313935106 0.0020251177605977787 56-57 2.7001570141303716E-4 0.0 0.0 1.07988279518454 0.0020251177605977787 58-59 2.7001570141303716E-4 0.0 0.0 1.2197959311333952 0.0020251177605977787 60-61 2.7001570141303716E-4 0.0 0.0 1.3718147710289355 0.0020476190690488652 62-63 2.7001570141303716E-4 0.0 0.0 1.5184107955877635 0.0021151229944021245 64-65 2.7001570141303716E-4 0.0 0.0 1.6681795046381946 0.0021601256113042973 66-67 2.7001570141303716E-4 0.0 0.0 1.8418671045721309 0.0021601256113042973 68-69 2.7001570141303716E-4 0.0 0.0 2.030585578551393 0.0021601256113042973 70-71 2.7001570141303716E-4 0.0 0.0 2.2283495785279914 0.002182626919755384 72-73 2.7001570141303716E-4 0.0 0.0 2.4309513598215737 0.0022726321535597293 74-75 2.7001570141303716E-4 0.0 0.0 2.632270566533444 0.0023401360789129886 76-77 2.7001570141303716E-4 0.0 0.0 2.8469555504652595 0.0023401360789129886 78-79 2.7001570141303716E-4 0.0 0.0 3.0876745482749826 0.0023851386958151618 80-81 2.7001570141303716E-4 0.0 0.0 3.3397117042356013 0.0023851386958151618 82-83 2.7001570141303716E-4 0.0 0.0 3.6010193992780684 0.002475143929619507 84-85 2.7001570141303716E-4 0.0 0.0 3.864577225165643 0.0024976452380705938 86-87 2.7001570141303716E-4 0.0 0.0 4.136258023404061 0.002565149163423853 88-89 2.7001570141303716E-4 0.0 0.0 4.416421814928539 0.002610151780326026 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3670 0.0 53.59149 1 GTATCAA 6635 0.0 42.162018 1 TATCAAC 8505 0.0 32.80396 2 ATCAACG 8475 0.0 32.695747 3 TCAACGC 8530 0.0 32.59637 4 CAACGCA 8635 0.0 32.20001 5 AACGCAG 8920 0.0 31.272125 6 ACGCAGA 10395 0.0 26.688 7 CGCAGAG 10510 0.0 26.39598 8 GCAGAGT 11515 0.0 23.967907 9 TAAGGTG 1360 0.0 23.764706 5 GTACATG 7230 0.0 23.571901 1 TGGTATC 1775 0.0 23.563877 2 TACATGG 7100 0.0 23.095278 2 GTGGTAT 1960 0.0 22.407656 1 GTACCGT 170 3.139201E-6 22.362753 6 GAGTACT 7735 0.0 21.95501 12-13 ACATGGG 7060 0.0 21.94701 3 GAGTACA 5275 0.0 21.900679 1 GTGTAAG 975 0.0 21.543327 1 >>END_MODULE