FastQCFastQC Report
Thu 26 May 2016
SRR937930_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937930_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2222093
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86890.39102773826298No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70680.31807849626455775No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46330.20849712410776688No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA39900.17956044143966973No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA39650.1784353760171154No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA34980.15741915392380065No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA34950.15728414607309416No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG28220.12699738489793183No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC26460.1190769243231494No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA25480.11466666786673645No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA25100.11295656842445388No Hit
CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG23440.10548613401869318No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA42800.053.611131
GTATCAA73400.041.746041
ATCAACG92100.033.0075533
TCAACGC93500.032.815184
TATCAAC93150.032.7418942
CAACGCA94350.032.4692085
AACGCAG97450.031.4850646
ACGCAGA111850.027.2192367
TAAGGTG14850.026.866235
GTAAGGT13800.026.8453714
CGCAGAG112800.026.7794698
AGGTAAG13500.024.2810232
GCAGAGT125400.023.7099559
GTACATG70550.023.2986491
TACATGG69950.022.887262
GTACCGT2303.6343408E-922.7153156
GAGTACT83850.022.43086612-13
GGTAAGG16150.022.3529113
CAGAGTA119600.021.58351910-11
ACATGGG69550.021.513273
Produced by FastQC (version 0.11.5)