##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937930_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2222093 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.306472771391654 33.0 31.0 34.0 31.0 34.0 2 32.56210113618107 34.0 31.0 34.0 31.0 34.0 3 32.53723088997625 34.0 31.0 34.0 31.0 34.0 4 36.116707986569416 37.0 35.0 37.0 35.0 37.0 5 36.06180569400111 37.0 35.0 37.0 35.0 37.0 6 36.12458434457964 37.0 36.0 37.0 35.0 37.0 7 36.075649399012555 37.0 35.0 37.0 35.0 37.0 8 36.066364909119464 37.0 35.0 37.0 35.0 37.0 9 37.73797631332262 39.0 38.0 39.0 35.0 39.0 10-11 37.692471242202735 39.0 37.0 39.0 35.0 39.0 12-13 37.62620016353951 39.0 37.0 39.0 35.0 39.0 14-15 38.83628633005009 40.0 38.0 41.0 35.0 41.0 16-17 38.74720522498383 40.0 38.0 41.0 35.0 41.0 18-19 38.724087830707354 40.0 38.0 41.0 35.0 41.0 20-21 38.710618097442364 40.0 38.0 41.0 34.5 41.0 22-23 38.74229206428354 40.0 38.0 41.0 34.5 41.0 24-25 38.67392948899979 40.0 38.0 41.0 34.0 41.0 26-27 38.47201804784949 40.0 38.0 41.0 34.0 41.0 28-29 38.38015893124185 40.0 38.0 41.0 34.0 41.0 30-31 38.14098869849282 40.0 38.0 41.0 33.5 41.0 32-33 37.92077244291755 40.0 37.0 41.0 33.0 41.0 34-35 37.68779164508416 40.0 37.0 41.0 33.0 41.0 36-37 37.59308633797055 40.0 37.0 41.0 33.0 41.0 38-39 37.526592271340576 40.0 37.0 41.0 33.0 41.0 40-41 37.37864639328777 40.0 37.0 41.0 32.0 41.0 42-43 37.15709581012136 40.0 36.0 41.0 31.5 41.0 44-45 36.879093719299775 39.0 36.0 41.0 31.0 41.0 46-47 36.49606204600798 39.0 35.5 41.0 30.0 41.0 48-49 36.081040037478175 39.0 35.0 40.0 29.5 41.0 50-51 35.73263360264399 39.0 35.0 40.0 28.5 41.0 52-53 35.18550753726329 38.0 34.0 40.0 27.0 41.0 54-55 34.797549877525384 38.0 34.0 40.0 26.0 41.0 56-57 34.46598049676588 38.0 34.0 40.0 26.0 41.0 58-59 34.01660686568924 37.0 33.0 40.0 24.5 41.0 60-61 33.52424088460744 36.5 33.0 39.0 23.0 41.0 62-63 33.01685753026538 36.0 32.0 39.0 22.0 40.0 64-65 32.51338692844989 35.0 31.0 39.0 21.0 40.0 66-67 31.926927225818183 35.0 31.0 38.0 18.5 40.0 68-69 31.330539495871683 35.0 30.0 37.5 17.0 39.5 70-71 30.66039450194029 34.0 29.5 37.0 12.0 39.0 72-73 30.133572492240425 34.0 29.0 36.0 8.0 39.0 74-75 29.52869524362842 34.0 28.5 36.0 7.0 38.5 76-77 28.455279099479633 32.5 27.0 34.5 7.0 36.5 78-79 29.166727045177677 33.5 29.0 35.0 4.0 37.0 80-81 28.947069047065085 34.0 29.0 35.0 2.0 37.0 82-83 28.411759093791304 33.0 27.5 35.0 2.0 36.0 84-85 27.82661639274324 33.0 26.5 35.0 2.0 36.0 86-87 27.181685915035956 32.5 25.5 35.0 2.0 35.0 88-89 26.414495927938212 32.0 24.5 34.0 2.0 35.0 90-91 25.75576134752236 32.0 23.5 34.0 2.0 35.0 92-93 24.97720662456522 31.0 19.5 34.0 2.0 35.0 94-95 24.04804074356924 31.0 12.0 34.0 2.0 35.0 96-97 23.017272679406307 30.0 2.0 34.0 2.0 35.0 98-99 21.763822216261875 30.0 2.0 34.0 2.0 35.0 100-101 18.36125378190742 24.0 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.06173654108216198 1101 2 0.05586757617496829 1101 3 0.1826375028510938 1101 4 0.10810616586481814 1101 5 0.054351039243321964 1101 6 0.04805052183218095 1101 7 0.13842432634279334 1101 8 0.16738021271282122 1101 9 0.18949871573290267 1101 10-11 0.15705910709804982 1101 12-13 0.10770135953294613 1101 14-15 0.2765080091883334 1101 16-17 0.27303194059214064 1101 18-19 0.293636923605348 1101 20-21 0.20536031784510556 1101 22-23 0.22315142150887368 1101 24-25 0.18300947009876012 1101 26-27 0.30602981526651263 1101 28-29 0.26271620187306155 1101 30-31 0.32091123016228806 1101 32-33 0.3079512546406491 1101 34-35 0.24683350865308284 1101 36-37 0.26443780651805326 1101 38-39 0.453394230660237 1101 40-41 0.4338996388180334 1101 42-43 0.47373881584897504 1101 44-45 0.5800450694891381 1101 46-47 0.6462433572803334 1101 48-49 0.5799929298596211 1101 50-51 0.6040738970226585 1101 52-53 0.5561184894233406 1101 54-55 0.6083896065212926 1101 56-57 0.5953856591589854 1101 58-59 0.6990498018897355 1101 60-61 0.811770057339821 1101 62-63 0.868480102820449 1101 64-65 0.7961698040768965 1101 66-67 0.8374279893812151 1101 68-69 1.043480510691488 1101 70-71 1.0387180955583446 1101 72-73 0.9902379829516654 1101 74-75 1.1531269940798374 1101 76-77 1.1820942507075465 1101 78-79 1.3262268617455497 1101 80-81 1.5820016215063468 1101 82-83 1.6589415459237493 1101 84-85 1.8470258088032345 1101 86-87 1.913532045332996 1101 88-89 2.1927189501367437 1101 90-91 2.3969730197680583 1101 92-93 2.4982457771213724 1101 94-95 2.400406636952596 1101 96-97 2.5830308664683237 1101 98-99 2.949772591116922 1101 100-101 2.9646842305224936 1102 1 0.13357955172714497 1102 2 0.17144373933376755 1102 3 0.22573303540181655 1102 4 0.11729611240234306 1102 5 0.12079001201379924 1102 6 0.0759234396145203 1102 7 0.11902090233001417 1102 8 0.13866227189782165 1102 9 0.07109917394600274 1102 10-11 0.20266145569701877 1102 12-13 0.2507574662370118 1102 14-15 0.3902673086154351 1102 16-17 0.3280611760774619 1102 18-19 0.3029578615552282 1102 20-21 0.2944842421423459 1102 22-23 0.40858453434000097 1102 24-25 0.2350233346408075 1102 26-27 0.28009319456241144 1102 28-29 0.21403491296893407 1102 30-31 0.3362218146536762 1102 32-33 0.3863364515836736 1102 34-35 0.3015005914101181 1102 36-37 0.3500676633136166 1102 38-39 0.5173793618507077 1102 40-41 0.38802695990238334 1102 42-43 0.5190191438925638 1102 44-45 0.5547296948215958 1102 46-47 0.621830153859662 1102 48-49 0.5843365151973146 1102 50-51 0.6880198078431903 1102 52-53 0.6198625065190058 1102 54-55 0.6658450726516634 1102 56-57 0.7826821890423616 1102 58-59 0.7252593213695704 1102 60-61 0.9988707422194238 1102 62-63 0.8494796058236176 1102 64-65 0.7530460184764536 1102 66-67 0.7741636320160943 1102 68-69 0.985757603760888 1102 70-71 1.196406713560414 1102 72-73 1.0865083235421835 1102 74-75 1.1780105465669415 1102 76-77 1.298403387872252 1102 78-79 1.514634892289667 1102 80-81 1.7533810895014206 1102 82-83 1.7440644531111964 1102 84-85 1.8568989654522703 1102 86-87 1.968020299127577 1102 88-89 2.058383725133311 1102 90-91 2.2659308803443956 1102 92-93 2.493084779685976 1102 94-95 2.4747702691133 1102 96-97 2.712435613237524 1102 98-99 3.0759930907592654 1102 100-101 3.303801412614682 1103 1 0.1659669265490109 1103 2 0.21129639080714213 1103 3 0.3377981420856955 1103 4 0.20558629428500552 1103 5 0.007827493551985754 1103 6 0.1418340098278037 1103 7 0.1953127968635826 1103 8 0.17259578990336166 1103 9 0.2238846103584322 1103 10-11 0.2527308158964843 1103 12-13 0.1637820466699793 1103 14-15 0.2898253062843281 1103 16-17 0.25359383862252116 1103 18-19 0.39317315388440477 1103 20-21 0.4392240575998869 1103 22-23 0.4099841723478761 1103 24-25 0.40814271635652233 1103 26-27 0.4126682149433947 1103 28-29 0.34294054633621585 1103 30-31 0.4645773778498423 1103 32-33 0.5217315614463374 1103 34-35 0.473473070061452 1103 36-37 0.47661272527827947 1103 38-39 0.4691556425005601 1103 40-41 0.6175608355751692 1103 42-43 0.595215620848542 1103 44-45 0.7414464868015429 1103 46-47 0.6642742169942011 1103 48-49 0.6642510340224206 1103 50-51 0.7962901061082661 1103 52-53 0.8191471179613288 1103 54-55 0.8555341356888988 1103 56-57 0.8665772156511622 1103 58-59 1.0882542764016705 1103 60-61 1.007692678423183 1103 62-63 0.9857796959972873 1103 64-65 0.9934666883564987 1103 66-67 1.0187797019579854 1103 68-69 1.2159037196978346 1103 70-71 1.1620239490564046 1103 72-73 0.9918851716157739 1103 74-75 1.0512143714873083 1103 76-77 0.9462049452601171 1103 78-79 1.4907002173797856 1103 80-81 1.5697482044414883 1103 82-83 1.7371733037537638 1103 84-85 1.7999284546195327 1103 86-87 1.740413426034575 1103 88-89 2.1698249684486726 1103 90-91 2.115483039430636 1103 92-93 2.433826409429013 1103 94-95 2.505489863612919 1103 96-97 2.783198964670465 1103 98-99 2.9507335981969725 1103 100-101 3.045383582015752 1104 1 0.11282708115756179 1104 2 0.0816616925645306 1104 3 0.1456919962615899 1104 4 0.13674398723964032 1104 5 0.02638370411978741 1104 6 0.06188897976502972 1104 7 0.0690824924338429 1104 8 0.039892857476672816 1104 9 0.08618531572135879 1104 10-11 0.19605606912346474 1104 12-13 0.05219148163316589 1104 14-15 0.20536563498922789 1104 16-17 0.19773286687983926 1104 18-19 0.12883896891350588 1104 20-21 0.18700617540056896 1104 22-23 0.2056103150666786 1104 24-25 0.17846482247249185 1104 26-27 0.16457780566966562 1104 28-29 0.286541122012693 1104 30-31 0.34940413745017196 1104 32-33 0.2525769319982558 1104 34-35 0.2988543163851034 1104 36-37 0.3950019508520839 1104 38-39 0.22623701552478082 1104 40-41 0.3591910579929163 1104 42-43 0.2952493861051124 1104 44-45 0.33403345784155647 1104 46-47 0.36339953013771975 1104 48-49 0.28922975967137887 1104 50-51 0.47249535408010956 1104 52-53 0.3353662601994003 1104 54-55 0.37284697862894234 1104 56-57 0.5969732121915285 1104 58-59 0.6393097330363346 1104 60-61 0.6734112886780679 1104 62-63 0.5781700970100943 1104 64-65 0.6948995843256682 1104 66-67 0.5724000242133975 1104 68-69 0.8363712876339378 1104 70-71 0.8849189328878992 1104 72-73 0.8566602955046569 1104 74-75 1.0424627752889215 1104 76-77 0.8863448931665694 1104 78-79 1.0416960809492402 1104 80-81 1.0850357540443198 1104 82-83 1.2604780490039822 1104 84-85 1.2987191757180199 1104 86-87 1.4325255436063422 1104 88-89 1.5991674989369322 1104 90-91 1.4587567614517525 1104 92-93 1.7258262083801732 1104 94-95 1.7805378615347536 1104 96-97 2.2980978530296348 1104 98-99 2.627212551269981 1104 100-101 2.803256833662214 1105 1 0.09889148742657738 1105 2 0.11665589586745284 1105 3 0.16604049473121307 1105 4 0.13857788731524323 1105 5 0.07737273357655994 1105 6 0.03834650749033841 1105 7 0.13153133256476224 1105 8 0.04680364795152059 1105 9 0.12898068647925243 1105 10-11 0.06463946121377973 1105 12-13 0.08174260702909208 1105 14-15 0.23168122129052193 1105 16-17 0.18086642647916307 1105 18-19 0.3488743003073722 1105 20-21 0.21049612997680356 1105 22-23 0.22820262098146316 1105 24-25 0.23671206717856563 1105 26-27 0.12373007844713868 1105 28-29 0.14751312855324983 1105 30-31 0.2435123878279839 1105 32-33 0.20391852129333898 1105 34-35 0.30916600937435135 1105 36-37 0.334033481400958 1105 38-39 0.270944658923689 1105 40-41 0.3178292857423344 1105 42-43 0.23432230239585294 1105 44-45 0.27793028184047586 1105 46-47 0.42669260349318705 1105 48-49 0.4208708919637303 1105 50-51 0.3471916267239905 1105 52-53 0.4130214428164507 1105 54-55 0.5612691692148672 1105 56-57 0.5757264366435564 1105 58-59 0.7689536666316954 1105 60-61 0.6306977916654688 1105 62-63 0.6615577884056876 1105 64-65 0.8942646677946939 1105 66-67 0.8374080113922595 1105 68-69 1.0243357213917008 1105 70-71 1.1346550426748436 1105 72-73 0.9926332850688055 1105 74-75 0.7883676965294342 1105 76-77 0.7870500846622583 1105 78-79 1.0525225371572873 1105 80-81 1.097711371881374 1105 82-83 1.147511492094818 1105 84-85 1.361526303258465 1105 86-87 1.6303337690808952 1105 88-89 1.8870004858330027 1105 90-91 1.9435707976281016 1105 92-93 2.133225296744751 1105 94-95 2.0249685167168323 1105 96-97 2.194120530620701 1105 98-99 2.4747060027169674 1105 100-101 2.1954893493533376 1106 1 0.07488708762841156 1106 2 0.05846662026742422 1106 3 0.10985122561418592 1106 4 0.03138575163031021 1106 5 0.01984256330048595 1106 6 -0.03897951301540559 1106 7 -0.012719810334672843 1106 8 -0.018154423225368532 1106 9 -0.071166909893293 1106 10-11 0.05018290435837969 1106 12-13 -0.0514844590770096 1106 14-15 0.011783155710624271 1106 16-17 0.04905913807088069 1106 18-19 0.0014255781613172758 1106 20-21 0.07786279891251979 1106 22-23 0.07166402604273969 1106 24-25 0.016088175366924418 1106 26-27 -0.01888626714880104 1106 28-29 -0.03089722826043584 1106 30-31 0.05531755845522923 1106 32-33 0.03895256246256196 1106 34-35 0.07956450877522059 1106 36-37 0.10777099756794684 1106 38-39 0.2609081596157452 1106 40-41 0.36055578836740665 1106 42-43 0.28112202189791446 1106 44-45 0.3654265135948762 1106 46-47 0.08104345498163212 1106 48-49 0.3351388474514252 1106 50-51 0.25789474083457264 1106 52-53 0.3883692829499168 1106 54-55 0.3737004898998606 1106 56-57 0.4408118988330614 1106 58-59 0.5880805789825772 1106 60-61 0.6794008128530109 1106 62-63 0.6064355135630848 1106 64-65 0.576848187784492 1106 66-67 0.7041617664017288 1106 68-69 0.25614711570199944 1106 70-71 0.5602971571279411 1106 72-73 0.7461854305646831 1106 74-75 0.8593730590535706 1106 76-77 0.8532099425548303 1106 78-79 1.001387849524587 1106 80-81 1.1563266348157768 1106 82-83 1.1912947242392775 1106 84-85 1.2266466115900911 1106 86-87 1.465302169842861 1106 88-89 1.5599942934844577 1106 90-91 1.5165029627242568 1106 92-93 1.3907209990538867 1106 94-95 1.7147732918808778 1106 96-97 2.0400233266319283 1106 98-99 2.45606655818068 1106 100-101 2.4090934993397752 1107 1 0.07322955758213823 1107 2 0.05128528981243363 1107 3 0.10222821799118265 1107 4 0.07495972103548354 1107 5 0.07800038667569709 1107 6 0.009031787681315961 1107 7 -0.02826074966078096 1107 8 0.008250675558244325 1107 9 0.028193792536463036 1107 10-11 0.07104027559938686 1107 12-13 0.06799993777311641 1107 14-15 0.10925715932274471 1107 16-17 0.09800049532426414 1107 18-19 0.04704000037420997 1107 20-21 0.0510761952716976 1107 22-23 0.07328929876776158 1107 24-25 0.06908863795818121 1107 26-27 0.08739628926163334 1107 28-29 0.11228743781425266 1107 30-31 0.05853451729878856 1107 32-33 -0.04635600008384699 1107 34-35 0.17293226173376297 1107 36-37 0.12404136000198918 1107 38-39 0.10351042152746714 1107 40-41 0.1435399627838052 1107 42-43 0.09964056010954891 1107 44-45 0.04536689072195088 1107 46-47 0.12940382597627575 1107 48-49 0.3041664132820614 1107 50-51 0.3623116897528007 1107 52-53 0.23991364651474356 1107 54-55 0.3725724584649228 1107 56-57 0.45630976931814615 1107 58-59 0.43877792200728294 1107 60-61 0.457808435506152 1107 62-63 0.5796162095059287 1107 64-65 0.5645358163928407 1107 66-67 0.6764167481196246 1107 68-69 0.7130393709318525 1107 70-71 0.5428197150980694 1107 72-73 0.6133309057664285 1107 74-75 0.8077422919496549 1107 76-77 0.9267487479350649 1107 78-79 1.0663638426335744 1107 80-81 1.152459744120243 1107 82-83 1.2120855426004304 1107 84-85 1.3233272091658321 1107 86-87 1.4345459660687574 1107 88-89 1.6851382550734328 1107 90-91 1.6656427379509893 1107 92-93 1.795897767402007 1107 94-95 1.7256671387082285 1107 96-97 1.722512918328679 1107 98-99 2.169412847613927 1107 100-101 2.288749763906523 1108 1 0.03010121777482766 1108 2 0.0397091948415067 1108 3 0.12912141831611734 1108 4 0.029127058966658126 1108 5 0.015296865669597537 1108 6 0.010745280907727306 1108 7 0.06686292922673687 1108 8 0.018946753412997452 1108 9 0.010378816049900763 1108 10-11 0.03464836282370953 1108 12-13 -0.006307722143397143 1108 14-15 0.10209887983973687 1108 16-17 0.12948574156965265 1108 18-19 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2316 62-63 -1.2007100471688759 2316 64-65 -1.0184336196886292 2316 66-67 -1.1014259205944121 2316 68-69 -1.1995364350049513 2316 70-71 -1.4563926795500066 2316 72-73 -1.4039550044655407 2316 74-75 -1.523445758532997 2316 76-77 -1.3748363833181152 2316 78-79 -1.5745696379264622 2316 80-81 -1.879703970823556 2316 82-83 -2.2028988001847445 2316 84-85 -2.121809133688352 2316 86-87 -2.133565506399563 2316 88-89 -2.219426491607493 2316 90-91 -2.652027917064064 2316 92-93 -3.0619071169588885 2316 94-95 -3.2165771483493337 2316 96-97 -3.137068607020481 2316 98-99 -3.715230573336548 2316 100-101 -4.0668493078751204 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 7.0 4 11.0 5 38.0 6 95.0 7 266.0 8 640.0 9 1141.0 10 1979.0 11 3134.0 12 4872.0 13 7168.0 14 9708.0 15 13075.0 16 16593.0 17 22667.0 18 22667.0 19 19035.0 20 18102.0 21 18615.0 22 19861.0 23 21903.0 24 24572.0 25 28426.0 26 33520.0 27 40277.0 28 49302.0 29 59477.0 30 71289.0 31 87460.0 32 109097.0 33 141322.0 34 189536.0 35 265098.0 36 359333.0 37 387746.0 38 167867.0 39 6190.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.78210092916903 18.31948527806892 14.1458075787107 23.752606214051347 2 16.345838794233288 19.452655797675458 39.16423926663114 25.037266141460112 3 20.242171396682238 22.634722184718132 29.272855741275798 27.850250677323828 4 13.468698204800608 15.887273844974084 35.29672250441363 35.34730544581167 5 14.009583787486173 36.83907823549583 33.99638897864439 15.1549489983736 6 34.34300158904956 35.003507969774304 16.03882483237575 14.614665608800392 7 30.03002124573544 32.16355031045055 19.304817575141993 18.501610868672014 8 27.37109894639826 34.720254606919966 18.602650115296758 19.30599633138502 9 26.378193891974817 16.355571076458094 19.445585760811994 37.8206492707551 10-11 25.8528558435673 25.854318428616622 26.283238370311235 22.009587357504838 12-13 26.511131622303836 23.009050476285196 27.089932779591138 23.389885121819834 14-15 22.836667952241424 24.63517503542831 25.25027080324721 27.277886209083057 16-17 22.6994549733067 27.87684853874253 26.33267374497827 23.091022742972502 18-19 22.871657486882864 26.088962073144558 28.607196008447893 22.432184431524693 20-21 22.941929073175604 25.263186554298134 29.25338858454619 22.54149578798007 22-23 22.737752200290448 25.294350866502885 29.066897740103588 22.90099919310308 24-25 22.693904791821275 25.583344621672623 29.047432176582326 22.67531840992378 26-27 22.187630394535887 25.94974484869845 28.902856155162237 22.95976860160342 28-29 22.400938217723844 26.39455142477641 28.704588549810527 22.49992180768922 30-31 22.464184064510423 25.877343879558463 28.63087709523982 23.027594960691296 32-33 22.055953843592064 25.865095116583635 28.749173046025895 23.32977799379841 34-35 22.982182646607598 26.0341394813116 28.524891655791173 22.45878621628963 36-37 23.13614607219448 25.506128722295855 28.455746506954586 22.901978698555077 38-39 22.51351143323594 25.877584861973403 28.4794275572472 23.129476147543457 40-41 22.998555993078853 25.768163968606355 28.573399483974583 22.659880554340216 42-43 22.664086642365287 25.797362316746597 28.334806314758016 23.203744726130104 44-45 22.613630175180557 26.108397441984916 28.68342515501519 22.594547227819337 46-47 23.22544177753296 26.320899747349962 27.82831678552109 22.625341689595984 48-49 22.815910714587073 26.47616261735868 27.668127380222423 23.039799287831826 50-51 22.98409652521294 26.739969929251384 27.681064653909626 22.59486889162605 52-53 23.419586848975268 26.23634564349917 27.737610442047206 22.60645706547836 54-55 22.775644403722076 26.568824977172422 27.8773660688369 22.7781645502686 56-57 22.891616147478977 26.80178102356652 27.508682129865853 22.797920699088653 58-59 22.81814937538618 26.285128480221125 27.564822894451314 23.331899249941383 60-61 22.447710334355943 26.61457013725348 27.916045818064323 23.021673710326255 62-63 22.623333046816672 26.634146275605925 28.039780513236845 22.702740164340558 64-65 22.273190186009316 26.69240216318579 27.861592651612693 23.172814999192205 66-67 22.214949279663667 26.704399962737735 27.765024501740932 23.31562625585767 68-69 22.93050500250215 26.236449681560764 27.898422564722907 22.934622751214174 70-71 22.588433517409037 26.545693632084706 27.46174440043688 23.404128450069372 72-73 22.59846910097822 26.65806516648943 27.48924099936411 23.254224733168233 74-75 22.592101230686566 27.044525139136844 27.307092907452567 23.056280722724026 76-77 22.67666114784575 26.587861084122043 27.575150995030363 23.16032677300185 78-79 22.724116407369088 26.80677631404266 27.589484328513702 22.879622950074545 80-81 22.93863500997597 26.816471613767135 27.24840741113263 22.996485965124265 82-83 22.716146722848592 26.92101543292799 27.405744291238744 22.957093552984674 84-85 22.620896832227906 27.109063394821074 27.308411951012324 22.961627821938695 86-87 22.920825218742372 27.0943970226061 26.98999022311328 22.99478753553825 88-89 22.635859054408876 27.30175602579822 26.73431764623554 23.32806727355737 90-91 22.622429944396565 27.14444516952185 26.979165025866514 23.25395986021508 92-93 22.812922818120676 26.75269607903803 27.23283407652191 23.201547026319382 94-95 22.510101980613737 26.504451441863942 27.417128656862978 23.568317920659343 96-97 22.880074308471514 26.16029919600412 27.293062360027516 23.666564135496852 98-99 22.47949508784412 26.61535460205361 26.968175674292148 23.936974635810124 100-101 22.883231035481383 26.040824482358317 27.227647579943426 23.848296902216877 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 619.0 1 452.5 2 435.5 3 618.5 4 509.0 5 397.5 6 811.0 7 1798.0 8 2855.0 9 3216.5 10 2637.5 11 2020.0 12 1917.5 13 2062.0 14 2277.5 15 2450.0 16 2599.5 17 2762.5 18 3021.0 19 3329.0 20 3773.5 21 4430.0 22 5306.5 23 6482.5 24 8011.0 25 10110.5 26 12871.0 27 16046.5 28 18780.0 29 21340.5 30 24093.0 31 28613.5 32 36121.5 33 38332.5 34 36833.0 35 38799.5 36 42368.5 37 46124.5 38 49354.0 39 52081.0 40 55024.5 41 59588.5 42 62278.0 43 64492.0 44 68142.5 45 73342.0 46 76816.5 47 79409.0 48 81624.0 49 81115.5 50 81336.0 51 80695.0 52 80525.0 53 83130.0 54 85615.0 55 81904.5 56 75375.0 57 69955.0 58 65063.0 59 58197.0 60 47303.5 61 39257.5 62 33995.5 63 25875.0 64 18455.0 65 13988.5 66 10428.0 67 8045.0 68 6282.0 69 4806.5 70 3677.5 71 2582.0 72 1881.0 73 1575.5 74 1238.5 75 924.0 76 662.5 77 423.5 78 286.5 79 181.0 80 89.0 81 53.0 82 36.5 83 22.0 84 14.0 85 9.5 86 8.0 87 6.5 88 4.0 89 0.5 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.010665620205814968 3 0.005085295709945533 4 0.0 5 3.1501831831521004E-4 6 4.5002616902172865E-4 7 0.0 8 4.500261690217286E-5 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0016200942084782228 26-27 0.021151229944021244 28-29 3.3751962676629646E-4 30-31 1.1250654225543216E-4 32-33 0.0037352172028803474 34-35 0.01595342769182028 36-37 0.008010465808586768 38-39 0.020521193307390825 40-41 0.022366300600379915 42-43 0.018383569004537616 44-45 0.009630560017064992 46-47 0.0021151229944021245 48-49 2.4751439296195074E-4 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 2.7001570141303716E-4 68-69 2.2501308451086433E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 1.5750915915760502E-4 82-83 0.0011700680394564943 84-85 0.004747776083179237 86-87 6.975405619836794E-4 88-89 6.750392535325929E-5 90-91 0.004860282625434669 92-93 0.0010575614972010623 94-95 1.8001046760869144E-4 96-97 2.0251177605977786E-4 98-99 1.5750915915760502E-4 100-101 0.003082679257798841 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2222093.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.60920477213696 #Duplication Level Percentage of deduplicated Percentage of total 1 76.28679486009266 40.89674407064713 2 13.240904753641392 14.19668748612646 3 4.291982021109796 6.902692291440157 4 1.9543068761545754 4.190753500454638 5 1.1206975767248843 3.0039852939141 6 0.6886702816052024 2.2151439688235115 7 0.4501937158852281 1.6894168971414307 8 0.3183109217772874 1.3651516309413019 9 0.24954705504251165 1.2040217256651966 >10 1.2721603154954666 12.271521425694363 >50 0.08205597978514004 2.9864802900919445 >100 0.03815991160592642 3.935945732196169 >500 0.002771880076190671 1.075176148286865 >1k 0.003275858271861641 3.3567452349140705 >5k 1.6799273189034057E-4 0.7095343036627676 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8689 0.39102773826298 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7068 0.31807849626455775 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4633 0.20849712410776688 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 3990 0.17956044143966973 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 3965 0.1784353760171154 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 3498 0.15741915392380065 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 3495 0.15728414607309416 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 2822 0.12699738489793183 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 2646 0.1190769243231494 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 2548 0.11466666786673645 No Hit GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA 2510 0.11295656842445388 No Hit CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG 2344 0.10548613401869318 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 4.500261690217286E-5 2 0.0 0.0 0.0 0.0 4.500261690217286E-5 3 0.0 0.0 0.0 4.500261690217286E-5 4.500261690217286E-5 4 0.0 0.0 0.0 4.500261690217286E-5 4.500261690217286E-5 5 0.0 0.0 0.0 4.500261690217286E-5 4.500261690217286E-5 6 0.0 0.0 0.0 4.500261690217286E-5 1.3500785070651858E-4 7 4.500261690217286E-5 0.0 0.0 4.500261690217286E-5 1.3500785070651858E-4 8 4.500261690217286E-5 0.0 0.0 4.500261690217286E-5 1.3500785070651858E-4 9 4.500261690217286E-5 0.0 0.0 4.500261690217286E-5 1.8001046760869144E-4 10-11 4.500261690217286E-5 0.0 0.0 4.500261690217286E-5 2.0251177605977786E-4 12-13 4.500261690217286E-5 0.0 0.0 4.500261690217286E-5 3.3751962676629646E-4 14-15 4.500261690217286E-5 0.0 0.0 4.500261690217286E-5 4.050235521195557E-4 16-17 4.500261690217286E-5 0.0 0.0 4.500261690217286E-5 4.500261690217286E-4 18-19 4.500261690217286E-5 0.0 0.0 4.500261690217286E-5 4.500261690217286E-4 20-21 4.500261690217286E-5 0.0 0.0 1.3500785070651858E-4 4.500261690217286E-4 22-23 4.500261690217286E-5 0.0 0.0 1.8001046760869144E-4 4.950287859239015E-4 24-25 4.500261690217286E-5 0.0 0.0 3.600209352173829E-4 4.950287859239015E-4 26-27 4.500261690217286E-5 0.0 0.0 7.875457957880251E-4 7.200418704347658E-4 28-29 4.500261690217286E-5 0.0 0.0 0.0029926740239944955 7.200418704347658E-4 30-31 4.500261690217286E-5 0.0 0.0 0.011048142449483437 7.200418704347658E-4 32-33 4.500261690217286E-5 0.0 0.0 0.027676609394836307 7.650444873369387E-4 34-35 9.000523380434572E-5 0.0 0.0 0.05411564682486287 8.100471042391115E-4 36-37 9.000523380434572E-5 0.0 0.0 0.09893825325942704 8.100471042391115E-4 38-39 9.000523380434572E-5 0.0 0.0 0.1681522780549689 9.675562633967166E-4 40-41 9.000523380434572E-5 0.0 0.0 0.23952642846181504 9.90057571847803E-4 42-43 9.000523380434572E-5 0.0 0.0 0.31283569139545464 0.001035060188749976 44-45 9.000523380434572E-5 0.0 0.0 0.39766562425605045 0.0010800628056521488 46-47 1.3500785070651858E-4 0.0 0.0 0.49786395078873835 0.0011250654225543216 48-49 1.3500785070651858E-4 0.0 0.0 0.6041601319116706 0.0011250654225543216 50-51 1.3500785070651858E-4 0.0 0.0 0.7195243403403908 0.0011250654225543216 52-53 1.3500785070651858E-4 0.0 0.0 0.8254605005281057 0.0013500785070651858 54-55 1.3500785070651858E-4 0.0 0.0 0.9415672521357117 0.0013500785070651858 56-57 1.3500785070651858E-4 0.0 0.0 1.0759000635886977 0.0013500785070651858 58-59 1.3500785070651858E-4 0.0 0.0 1.2151381602840206 0.0013500785070651858 60-61 1.3500785070651858E-4 0.0 0.0 1.3661219399908107 0.0013950811239673588 62-63 1.3500785070651858E-4 0.0 0.0 1.5114128886594758 0.0015300889746738774 64-65 1.3500785070651858E-4 0.0 0.0 1.659944025745097 0.0015525902831249638 66-67 1.3500785070651858E-4 0.0 0.0 1.8316290092268865 0.0015750915915760501 68-69 1.3500785070651858E-4 0.0 0.0 2.017129796097643 0.0015750915915760501 70-71 1.3500785070651858E-4 0.0 0.0 2.2133412057911164 0.0016425955169293094 72-73 1.3500785070651858E-4 0.0 0.0 2.4138053627818454 0.0017326007507336553 74-75 1.3500785070651858E-4 0.0 0.0 2.6139545014542596 0.0017551020591847417 76-77 1.3500785070651858E-4 0.0 0.0 2.8289095010874883 0.0017551020591847417 78-79 1.3500785070651858E-4 0.0 0.0 3.069425987121151 0.0017551020591847417 80-81 1.3500785070651858E-4 0.0 0.0 3.3215306470071235 0.0017551020591847417 82-83 1.3500785070651858E-4 0.0 0.0 3.5820957988707045 0.0018451072929890873 84-85 1.3500785070651858E-4 0.0 0.0 3.8463061627033612 0.0018451072929890873 86-87 1.3500785070651858E-4 0.0 0.0 4.117536934772756 0.0018451072929890873 88-89 1.3500785070651858E-4 0.0 0.0 4.397475713212723 0.001867608601440174 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4280 0.0 53.61113 1 GTATCAA 7340 0.0 41.74604 1 ATCAACG 9210 0.0 33.007553 3 TCAACGC 9350 0.0 32.81518 4 TATCAAC 9315 0.0 32.741894 2 CAACGCA 9435 0.0 32.469208 5 AACGCAG 9745 0.0 31.485064 6 ACGCAGA 11185 0.0 27.219236 7 TAAGGTG 1485 0.0 26.86623 5 GTAAGGT 1380 0.0 26.845371 4 CGCAGAG 11280 0.0 26.779469 8 AGGTAAG 1350 0.0 24.281023 2 GCAGAGT 12540 0.0 23.709955 9 GTACATG 7055 0.0 23.298649 1 TACATGG 6995 0.0 22.88726 2 GTACCGT 230 3.6343408E-9 22.715315 6 GAGTACT 8385 0.0 22.430866 12-13 GGTAAGG 1615 0.0 22.352911 3 CAGAGTA 11960 0.0 21.583519 10-11 ACATGGG 6955 0.0 21.51327 3 >>END_MODULE