FastQCFastQC Report
Thu 26 May 2016
SRR937929_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937929_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2282055
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89420.3918398110474989No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73450.3218590261847326No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46450.20354461220259812No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA43080.18877722053149465No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA42210.1849648671920703No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA36110.15823457366277324No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA35000.15337053664350772No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG28710.12580766020100304No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA27670.12125036425502451No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA27660.12120654410169782No Hit
CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG25320.11095262822324616No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC25140.11016386546336525No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA39800.053.969441
GTATCAA72250.042.546761
ATCAACG89000.034.387063
TCAACGC89500.034.1949544
TATCAAC89800.034.1336362
CAACGCA91900.033.250235
AACGCAG94700.032.2671176
TGGTATC18350.030.559422
GTGGTAT20600.029.4276081
ACGCAGA108900.028.160527
CGCAGAG109150.027.921788
GTACATG72500.025.08451
TACATGG71700.024.8548582
GCAGAGT123000.024.4663569
AGGCGAC9600.023.7612445
TAAGGTG13600.023.4118145
ACATGGG72250.023.2186013
GAGTACT82100.021.91425512-13
GTATAGG5900.021.8439031
CAGAGTA115750.021.48013910-11
Produced by FastQC (version 0.11.5)